CTLA-4 Antibody [Unconjugated]

Images

 
Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with human CTLA-4. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human CTLA‑4 Antigen Affinity-purified ...read more
NS0 mouse myeloma cell line co-transfected with human CTLA-4 and eGFP was stained with either (A) Goat Anti-Human CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-386-PB) or (B) Normal Goat IgG ...read more
CTLA‑4 was detected in immersion fixed human peripheral blood mononuclear cells treated with treated with PMA and calcium ionomycin using Goat Anti-Human CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

CTLA-4 Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CTLA-4
Ala37-Phe162
Accession # Q6GR94
Specificity
Detects human CTLA-4 in direct ELISAs and Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse CTLA-4 is observed.
Details of Functionality
ActivityAssaywCitation not found None None None
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CTLA4
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 2 Reviews rated 5
using
AF-386-PB in the following applications:

Publications
Read Publications using
AF-386-PB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CTLA-4 Antibody [Unconjugated]

  • CD
  • CD152 antigen
  • CD152
  • CD152IDDM12
  • CD28
  • celiac disease 3
  • CELIAC3
  • CTLA4
  • CTLA-4
  • cytotoxic T-lymphocyte antigen 4
  • cytotoxic T-lymphocyte protein 4
  • Cytotoxic T-lymphocyte-associated antigen 4
  • cytotoxic T-lymphocyte-associated protein 4
  • cytotoxic T-lymphocyte-associated serine esterase-4
  • GRD4
  • GSE
  • ICOS
  • ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4

Background

CTLA-4 and CD28, together with their ligands B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T- and B-cell responses. CTLA-4 and CD28 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Both CTLA-4 and CD28 are composed of a single
Ig V‑like extracellular domain, a transmembrane domain and an intracellular domain. CTLA-4 and CD28 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. The genes encoding these two molecules are closely linked on human chromosome 2. CTLA-4 was originally identified as a gene that was specifically expressed by cytotoxic T lymphocytes. However, CTLA-4 transcripts have since been found in both Th1 and Th2, and CD4+ and CD8+ T cell clones. Whereas CD28 expression is constitutive on the surfaces of 95% of CD4+ T cells and 50% of CD8+ T cells and is down regulated upon T cell activation, CTLA-4 expression is upregulated rapidly following T cell activation and peaks approximately 24 hours following activation. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with 20‑100‑fold higher affinity than CD28. The physiological role of CTLA-4 in T cell costimulation is currently being studied.

  1. Lenschow, D.J. et al. (1996) Annu. Rev. Immunol. 14:233.
  2. Hathcock, K.S. and R.J. Hodes (1996) Advances in Immunol. 62:131.
  3. Ward, S.G. (1996) Biochem. J. 318:361.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CTLA-4 Antibody (AF-386-PB)(2)

Reviews for CTLA-4 Antibody (AF-386-PB) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using AF-386-PB:
Filter by Applications
ICC
(1)
IF
(1)
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Filter by Species
Human
(2)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
ICC Human 06/21/2017
View

Summary

ApplicationImmunocytochemistry
Sample Tested breast cancer tissue,Adult lung
SpeciesHuman
Immunofluorescence CTLA-4 AF-386-PB
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reviewed by:
Christopher England
IF Human 08/05/2016
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Summary

ApplicationImmunofluorescence
Sample TestedHuman lung cancer cell line MiaPaCa-2
SpeciesHuman
LotANN0515031

Blocking

Blocking Details5% Donkey serum in PBS for 1 hr at room temperature

Primary Anitbody

Dilution Ratio1:500 dilution in blocking buffer, incubation overnight at 4 degree Celcius

Secondary Antibody

Secondary DescriptionDonkey anti-Goat DyLight 488
Secondary Manufacturer Cat#NBP1-72843
Secondary Concentration1:5000

Details

Detection NotesConfocal microscopy with Nikon A1R
Fixation DetailsCold acetone for 15 min at room temperature, PBS 5 for min at room temperature, No antigen retrieval,
Wash DescriptionWash two times with PBST for 10 min, Wash two times with PBS for 10 min

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Video Protocols

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FAQs for CTLA-4 Antibody (AF-386-PB) (0)

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Blogs on CTLA-4.

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Here at Bio-Techne we always look forward to the annual announcements of winners of the highly coveted Nobel Prize – the greatest award in science. How can you go about predicting which scientists might be in line for a life-changing phone-call fro...  Read full blog post.

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CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated...  Read full blog post.

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Recent Reviews

5
5
2
4
0
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2
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1
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Anonymous
06/21/2017
Application: ICC
Species: Human

Christopher England
08/05/2016
Application: IF
Species: Human

Bioinformatics

Gene Symbol CTLA4
Entrez
Uniprot