CTLA-4 Antibody [Unconjugated] Summary
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CTLA-4
Accession # Q6GR94
Detects human CTLA-4 in direct ELISAs and Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse CTLA-4 is observed.
|Details of Functionality
ActivityAssaywCitation not found None None None
Test in a species/application not listed above to receive a full credit towards a future purchase.
- Western Blot 0.5 ug/mL
- Flow Cytometry 0.25 ug/10^6 cells
- Immunocytochemistry 5-15 ug/mL
Packaging, Storage & Formulations
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for CTLA-4 Antibody [Unconjugated]
- CD152 antigen
- celiac disease 3
- cytotoxic T-lymphocyte antigen 4
- cytotoxic T-lymphocyte protein 4
- Cytotoxic T-lymphocyte-associated antigen 4
- cytotoxic T-lymphocyte-associated protein 4
- cytotoxic T-lymphocyte-associated serine esterase-4
- ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4
CTLA-4 and CD28, together with their ligands B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T- and B-cell responses. CTLA-4 and CD28 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Both CTLA-4 and CD28 are composed of a single
Ig V‑like extracellular domain, a transmembrane domain and an intracellular domain. CTLA-4 and CD28 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. The genes encoding these two molecules are closely linked on human chromosome 2. CTLA-4 was originally identified as a gene that was specifically expressed by cytotoxic T lymphocytes. However, CTLA-4 transcripts have since been found in both Th1 and Th2, and CD4+ and CD8+ T cell clones. Whereas CD28 expression is constitutive on the surfaces of 95% of CD4+ T cells and 50% of CD8+ T cells and is down regulated upon T cell activation, CTLA-4 expression is upregulated rapidly following T cell activation and peaks approximately 24 hours following activation. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with 20‑100‑fold higher affinity than CD28. The physiological role of CTLA-4 in T cell costimulation is currently being studied.
- Lenschow, D.J. et al. (1996) Annu. Rev. Immunol. 14:233.
- Hathcock, K.S. and R.J. Hodes (1996) Advances in Immunol. 62:131.
- Ward, S.G. (1996) Biochem. J. 318:361.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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