CTLA-4 Antibodies

HAF017). A specific band was detected for CTLA-4 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for CTLA-4 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with human CTLA-4. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human CTLA-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-386-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CTLA-4 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

AB-108-C) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108)." class="big_lightbox"> AB-108-C) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108)." class="big_thumb" />

434-CT) inhibits Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 10133-B1) induced IL‑2 secretion in the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IL‑2 Quantikine ELISA Kit (Catalog # D2050). Inhibition of Recombinant Human B7‑1/CD80 Fc Chimera (3 µg/mL) activity elicited by Recombinant Mouse CTLA‑4 Fc Chimera (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CTLA‑4 Monoclonal Antibody (Catalog # MAB434). The ND₅₀ is typically 2.5-10 µg/mL in the presence of PHA (10 µg/mL)." class="big_lightbox"> 434-CT) inhibits Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 10133-B1) induced IL‑2 secretion in the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IL‑2 Quantikine ELISA Kit (Catalog # D2050). Inhibition of Recombinant Human B7‑1/CD80 Fc Chimera (3 µg/mL) activity elicited by Recombinant Mouse CTLA‑4 Fc Chimera (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CTLA‑4 Monoclonal Antibody (Catalog # MAB434). The ND₅₀ is typically 2.5-10 µg/mL in the presence of PHA (10 µg/mL)." alt="Recombinant Mouse CTLA‑4 Fc Chimera (Catalog # 434-CT) inhibits Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 10133-B1) induced IL‑2 secretion in the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IL‑2 Quantikine ELISA Kit (Catalog # D2050). Inhibition of Recombinant Human B7‑1/CD80 Fc Chimera (3 µg/mL) activity elicited by Recombinant Mouse CTLA‑4 Fc Chimera (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CTLA‑4 Monoclonal Antibody (Catalog # MAB434). The ND₅₀ is typically 2.5-10 µg/mL in the presence of PHA (10 µg/mL)." class="big_thumb" />

434-CT) was serially diluted and captured by Goat Anti-Mouse CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF476) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF476) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 434-CT) was serially diluted and captured by Goat Anti-Mouse CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF476) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF476) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Mouse CTLA‑4 Fc Chimera (Catalog # 434-CT) was serially diluted and captured by Goat Anti-Mouse CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF476) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF476) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

434-CT) was serially diluted and captured by Goat Anti-Mouse CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF476) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF476) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 434-CT) was serially diluted and captured by Goat Anti-Mouse CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF476) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF476) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_lightbox"> DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." alt="CTLA4 was detected in immersion fixed paraffin-embedded sections of human Appendix using Rabbit Anti-Human CTLA4, Monoclonal Antibody (Catalog # NBP3-20446 ) at 1:100 at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module)and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />

DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_lightbox"> DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />

DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_lightbox"> DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />

HAF018). A specific band was detected for CTLA-4 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for CTLA-4 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of NS0 mouse myeloma cell line transfected with with Human CTLA-4 and mouse lymph node tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse CTLA-4 Monoclonal Antibody (Catalog # MAB386) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CTLA-4 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB100) coated at 1 µg/mL (100 µL/well) in a dose-dependent manner (green line) as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50C). The ED₅₀ is typically 0.05-0.4 µg/mL." class="big_lightbox"> MAB100) coated at 1 µg/mL (100 µL/well) in a dose-dependent manner (green line) as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50C). The ED₅₀ is typically 0.05-0.4 µg/mL." class="big_thumb" />

F0111). Quadrants were set based on Normal Rabbit IgG Control Antibody (Catalog # MAB1050, data not shown). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0111). Quadrants were set based on Normal Rabbit IgG Control Antibody (Catalog # MAB1050, data not shown). View our protocol for Staining Membrane-associated Proteins." alt="NS0 mouse myeloma cell line transfected with (A) human CTLA-4 or (B) irrelevant transfectants and eGFP was stained with Rabbit Anti-Human CTLA-4 Monoclonal Antibody (Catalog # MAB3252) followed by APC-conjugated Goat anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrants were set based on Normal Rabbit IgG Control Antibody (Catalog # MAB1050, data not shown). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

NL004) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL004) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

F0135). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0135). View our protocol for Staining Membrane-associated Proteins." alt="HEK293 Human cell line transfected with (A) Human CTLA-4 or (B) irrelevant transfectants and eGFP was stained with Human Anti-Human CTLA-4 (Research Grade Ipilimumab) Monoclonal Antibody (Catalog # MAB9928) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />