B7-1/CD80 Antibody (37711) [Unconjugated]


Raji human Burkitt's lymphoma cell line was stained with Mouse Anti-Human B7‑1/CD80 Monoclonal Antibody (Catalog # MAB140, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by ...read more
Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 140-B1) co-stimulates IL‑2 secretion in the Jurkat human acute T cell leukemia cell line in the presence of PHA in a dose-dependent manner (orange line), as measured ...read more
B7‑1/CD80 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human B7‑1/CD80 Monoclonal Antibody (Catalog # MAB140) at 5 µg/mL overnight at 4 °C. Tissue was stained using ...read more
Orthogonal Strategies: B7-1/CD80 mRNA was detected in formalin-fixed paraffin-embedded tissue sections of human tonsil probed with ACD RNAScope® Probe (Catalog # 42471) and stained using ACD ...read more
MNK inhibitors induce polarization of TAMs toward an M2 phenotype in ex vivo human PDAC slice cultures.(A) Serial sections of human PDAC tumors (n = 15) were stained for Arginase-1 and phosphorylated eIF4ES209 (p-eIF4E) ...read more

Product Details

Reactivity HuSpecies Glossary
Applications Flow, IHC, B/N, CyTOF-ready, Dual ISH-IHC, ELISA(Cap)
Validated by:

Orthogonal Strategies


Order Details

B7-1/CD80 Antibody (37711) [Unconjugated] Summary

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human B7‑1/CD80
Val35-Asn242 (predicted)
Accession # P33681
Detects human B7‑1/CD80 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) B7-2, recombinant mouse (rm) B7-1, rmB7-2, rhB7-H1, rhB7-H2, rhB7-H3 or rmPD-L2 is observed.
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • CyTOF-ready
  • Dual RNAscope ISH-IHC 5-25 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-25 ug/mL
  • Neutralization 0.2-1 ug/mL
Application Notes
ELISA Capture: Human B7-1/CD80 Antibody (Catalog # MAB140)
ELISA Detection: Human B7-1/CD80 Biotinylated Antibody (Catalog # BAM1402)
Standard: Recombinant Human B7-1/CD80 Fc Chimera (Catalog # 140-B1)
Read Publications using
MAB140 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for B7-1/CD80 Antibody (37711) [Unconjugated]

  • Activation B7-1 antigen
  • B7
  • B71
  • B7-1
  • BB1
  • CD28LG1
  • CD28LGB7-1 antigen)
  • CD80 antigen
  • CD80 molecule
  • CD80
  • costimulatory factor CD80
  • costimulatory molecule variant IgV-CD80
  • CTLA-4 counter-receptor B7.1
  • T-lymphocyte activation antigen CD80


B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20‑100 fold higher affinity than CD28 and is involved in the down‑regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up‑regulated through Interferon gamma . B7-1 and B7-2 are both members of the Immunoglobulin superfamily. Human B7-1 is a 288 amino acid (aa) protein containing a 34 aa signal peptide, a 208 aa extracellular domain, a 21 aa transmembrane domain, and a 25 aa cytoplasmic domain. Human B7-1 and B7-2 share 26% aa sequenceidentity. Human and mouse B7-1 share 44% aa sequenceidentity. However, it has been observed that both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form the B7-1/B7-2/CD28/CTLA-4 critical binding sites.

  1. Azuma, M. et al. (1993) Nature 366:76.
  2. Freeman, G.J. et al. (1993) Science 262:909.
  3. Freeman, G. et al. (1991) J. Exp. Med. 174:625.
  4. Selvakumar, A. et al. (1993) Immunogenetics 38:292.
  5. Chen, C. et al. (1994) J. Immunol. 152:4929.
  6. Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for B7-1/CD80 Antibody (MAB140)(14)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 9 applications: ELISA Development (Detection), Flow Cytometry, Functional Assay, ICC, IHC, IHC-P, Neutralization, T Cell Activation, Western Blot.

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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using MAB140 Applications Species
S Shankar, J Stolp, SC Juvet, J Beckett, PS Macklin, F Issa, J Hester, KJ Wood Ex vivo-expanded human CD19+TIM-1+ regulatory B cells suppress immune responses in vivo and are dependent upon the TIM-1/STAT3 axis Nature Communications, 2022-06-03;13(1):3121. 2022-06-03 [PMID: 35660734] (Neutralization, Human) Neutralization Human
TN Pham, C Spaulding, MA Shields, AE Metropulos, DN Shah, MG Khalafalla, DR Principe, DJ Bentrem, HG Munshi Inhibiting MNK kinases promotes macrophage immunosuppressive phenotype to limit CD8+ T cell anti-tumor immunity JCI Insight, 2022-05-09;0(0):. 2022-05-09 [PMID: 35380995] (IHC, Human) IHC Human
T Yamada, Y Miki, M Suzuki, O Kondoh, R Saito-Koya, K Ono, Y Okada, H Sasano B7-1 and programmed cell death-ligand 1 in primary and lymph node metastasis lesions of non-small cell lung carcinoma Cancer Medicine, 2021-12-14;0(0):. 2021-12-14 [PMID: 34907653] (IHC, Human) IHC Human
Y Liu, S He, XL Wang, W Peng, QY Chen, DM Chi, JR Chen, BW Han, GW Lin, YQ Li, QY Wang, RJ Peng, PP Wei, X Guo, B Li, X Xia, HQ Mai, XD Hu, Z Zhang, YX Zeng, JX Bei Tumour heterogeneity and intercellular networks of nasopharyngeal carcinoma at single cell resolution Nature Communications, 2021-02-02;12(1):741. 2021-02-02 [PMID: 33531485] (ICC, Human) ICC Human
B Diskin, S Adam, MF Cassini, G Sanchez, M Liria, B Aykut, C Buttar, E Li, B Sundberg, RD Salas, R Chen, J Wang, M Kim, MS Farooq, S Nguy, C Fedele, KH Tang, T Chen, W Wang, M Hundeyin, JAK Rossi, E Kurz, MIU Haq, J Karlen, E Kruger, Z Sekendiz, D Wu, SAA Shadaloey, G Baptiste, G Werba, S Selvaraj, C Loomis, KK Wong, J Leinwand, G Miller PD-L1 engagement on T cells promotes self-tolerance and suppression of neighboring macrophages and effector T cells in cancer Nat. Immunol., 2020-03-09;21(4):442-454. 2020-03-09 [PMID: 32152508] (T Cell Activation, Human, Mouse) T Cell Activation Human, Mouse
S Minamikawa, K Nozu, S Maeta, T Yamamura, K Nakanishi, J Fujimura, T Horinouchi, C Nagano, N Sakakibara, H Nagase, H Shima, K Noda, T Ninchoji, H Kaito, K Iijima The utility of urinary CD80 as a diagnostic marker in patients with renal diseases Sci Rep, 2018-11-23;8(1):17322. 2018-11-23 [PMID: 30470792] (ELISA Development (Detection), Human) ELISA Development (Detection) Human
J Li, SQ Xu, YM Zhao, S Yu, LH Ge, BH Xu Comparison of the biological characteristics of human mesenchymal stem cells derived from exfoliated deciduous teeth, bone marrow, gingival tissue, and umbilical cord Mol Med Rep, 2018-09-20;0(0):. 2018-09-20 [PMID: 30272340] (Flow Cytometry, Human) Flow Cytometry Human
S He, C Wu, J Xiao, D Li, Z Sun, M Li Endothelial extracellular vesicles modulate the macrophage phenotype: Potential implications in atherosclerosis Scand. J. Immunol., 2018-04-01;0(0):. 2018-04-01 [PMID: 29465752] (Flow Cytometry, Human) Flow Cytometry Human
SW Lee, SH Baek, JH Paik, S Kim, KY Na, DW Chae, HJ Chin Tubular B7-1 expression parallels proteinuria levels, but not clinical outcomes in adult minimal change disease patients Sci Rep, 2017-02-02;7(0):41859. 2017-02-02 [PMID: 28150736] (IHC-P, Human) IHC-P Human
Xiongbiao Wang Autoantibodies against CD80 in patients with COPD Clin Transl Immunology, 2016-10-07;5(10):e103. 2016-10-07 [PMID: 27867516] (Western Blot, Human) Western Blot Human
Show All 14 Publications.

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CD80: A co-stimulator of T cell activation
CD80 is a 60kD single chain type I transmembrane glycoprotein that is a member of the immunoglobulin family. CD80 is expressed on activated B- and T-lymphocytes, as well as a subpopulation of previously activated B-cells, but not on the majority of re...  Read full blog post.

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Gene Symbol CD80