B7-1/CD80 Antibody (37711) [Unconjugated]

Raji human Burkitt's lymphoma cell line was stained with Mouse Anti-Human B7-1/CD80 Monoclonal Antibody (Catalog # MAB140, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). View our protocol for Staining Membrane-associated Proteins.

Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 140-B1) co-stimulates IL‑2 secretion in the Jurkat human acute T cell leukemia cell line in the presence of PHA in a dose-dependent manner (orange line), as measured by the Human IL‑2 Quantikine ELISA Kit (Catalog # D2050). IL‑2 secretion elicited by Recombinant Human B7‑1/CD80 Fc Chimera (1 µg/mL) and PHA (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human B7‑1/CD80 Monoclonal Antibody (Catalog # MAB140). The ND50 is typically 0.2-1 µg/mL.

B7-1/CD80 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human B7-1/CD80 Monoclonal Antibody (Catalog # MAB140) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

B7-1/CD80 mRNA was detected in formalin-fixed paraffin-embedded tissue sections of human tonsil probed with ACD RNAScope® Probe (Catalog # 42471) and stained using ACD RNAscope® 2.5 HD Duplex Detection Reagents (red, Catalog # 322500). Adjacent tissue section was processed for immunohistochemistry using R&D Systems Mouse Anti-Human B7-1/CD80 Monoclonal Antibody (Catalog# MAB140) at 5ug/mL with overnight incubation at 4 °C followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC001) and DAB chromogen (lower image, brown). Tissues were counterstained with hematoxylin (blue).

MNK inhibitors induce polarization of TAMs toward an M2 phenotype in ex vivo human PDAC slice cultures.(A) Serial sections of human PDAC tumors (n = 15) were stained for Arginase-1 and phosphorylated eIF4ES209 (p-eIF4E) by IHC. Scale bar: 200 μm. The staining positivity was analyzed by the TissueGnostics Imaging system using the HistoQuest, and the correlation analysis for Arginase-1 and p-eIF4E expression was performed using GraphPad. (B) Slice cultures (n = 1–4 replicates for each treatment group) from 6 different PDAC tumors were treated with DMSO (vehicle control) or CGP57380 for 5 days and stained for phosphorylated eIF4E (p-eIF4E), CD68, Arginase-1, CD163, MRC1, CD86, CD80, and MHCII. The percentage of positive cells, relative to the total number of nucleated cells, was analyzed by ImageJ. Scale bar: 50 μm. Data points represent individual patients. Paired t test was performed by GraphPad. *P < 0.05; **P < 0.01. (C) The relative expression of MKNK2, CD163, and MRC1 in TCGA studies and their transcript abundance from RNA-Seq data, quantified as RSEM, were downloaded from cBioPortal. Correlation analysis was performed in GraphPad to evaluate the relationship between MKNK2, CD163, and MRC1. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35380995), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: Flow, IHC, B/N, CyTOF-ready, Dual ISH-IHC, ELISA(Cap)
• Clone: 37711
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

B7-1/CD80 Antibody (37711) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human B7‑1/CD80
  Val35-Asn242 (predicted)
  Accession # P33681
• Specificity: Detects human B7-1/CD80 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) B7-2, recombinant mouse (rm) B7-1, rmB7-2, rhB7-H1, rhB7-H2, rhB7-H3 or rmPD-L2 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD80
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Dual RNAscope ISH-IHC 5-25 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-25 ug/mL
  • Neutralization 0.2-1 ug/mL
• Application Notes: ELISA Capture: Human B7-1/CD80 Antibody (Catalog # MAB140) ELISA Detection: Human B7-1/CD80 Biotinylated Antibody (Catalog # BAM1402) Standard: Recombinant Human B7-1/CD80 Fc Chimera (Catalog # 140-B1)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for B7-1/CD80 Antibody (37711) [Unconjugated]

• Activation B7-1 antigen
• B7
• B71
• B7-1
• BB1
• CD28LG1
• CD28LGB7-1 antigen)
• CD80 antigen
• CD80 molecule
• CD80
• costimulatory factor CD80
• costimulatory molecule variant IgV-CD80
• CTLA-4 counter-receptor B7.1
• T-lymphocyte activation antigen CD80

Background

B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up-regulated through Interferon gamma . B7-1 and B7-2 are both members of the Immunoglobulin superfamily. Human B7-1 is a 288 amino acid (aa) protein containing a 34 aa signal peptide, a 208 aa extracellular domain, a 21 aa transmembrane domain, and a 25 aa cytoplasmic domain. Human B7-1 and B7-2 share 26% aa sequenceidentity. Human and mouse B7-1 share 44% aa sequenceidentity. However, it has been observed that both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form the B7-1/B7-2/CD28/CTLA-4 critical binding sites.

1. Azuma, M. et al. (1993) Nature 366:76.
2. Freeman, G.J. et al. (1993) Science 262:909.
3. Freeman, G. et al. (1991) J. Exp. Med. 174:625.
4. Selvakumar, A. et al. (1993) Immunogenetics 38:292.
5. Chen, C. et al. (1994) J. Immunol. 152:4929.
6. Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for B7-1/CD80 Antibody (MAB140)(26)

Publications using MAB140

• Yang, L;Han, Y;Zhang, T;Dong, X;Ge, J;Roy, A;Zhu, J;Lu, T;Vandana, JJ;de Silva, N;Robertson, CC;Xiang, JZ;Pan, C;Sun, Y;Que, J;Evans, T;Liu, C;Wang, W;Naji, A;Parker, SCJ;Schwartz, RE;Chen, S; Human Vascularized Macrophage-Islet Organoids to Model Immune-Mediated Pancreatic ? cell Pyroptosis upon Viral Infection bioRxiv : the preprint server for biology 2024-08-06 [PMID: 39149298] (Immunohistochemistry, Human)
• Ko, KJ;Kim, G;Sung, HH;Park, WY;Lee, KS; Potential Role of Macrophage Polarization in the Progression of Hunner-Type Interstitial Cystitis International journal of molecular sciences 2024-01-08 [PMID: 38255860] (IHC, Human)
• Morten Hjuler Nielsen, Rikke Bæk, Malene Moller Jorgensen, Maiken Mellergaard, Aase Handberg Increased extracellular vesicles (EVs) related to T cell-mediated inflammation and vascular function in familial hypercholesterolemia Atherosclerosis Plus 9/1/2023 [PMID: 37637934]
• S Shankar, J Stolp, SC Juvet, J Beckett, PS Macklin, F Issa, J Hester, KJ Wood Ex vivo-expanded human CD19+TIM-1+ regulatory B cells suppress immune responses in vivo and are dependent upon the TIM-1/STAT3 axis Nature Communications, 2022-06-03;13(1):3121. 2022-06-03 [PMID: 35660734] (Neutralization, Human)
• TN Pham, C Spaulding, MA Shields, AE Metropulos, DN Shah, MG Khalafalla, DR Principe, DJ Bentrem, HG Munshi Inhibiting MNK kinases promotes macrophage immunosuppressive phenotype to limit CD8+ T cell anti-tumor immunity JCI Insight, 2022-05-09;0(0):. 2022-05-09 [PMID: 35380995] (IHC, Human)
• T Yamada, Y Miki, M Suzuki, O Kondoh, R Saito-Koya, K Ono, Y Okada, H Sasano B7-1 and programmed cell death-ligand 1 in primary and lymph node metastasis lesions of non-small cell lung carcinoma Cancer Medicine, 2021-12-14;0(0):. 2021-12-14 [PMID: 34907653] (IHC, Human)
• Gota Sudo, Hironori Aoki, Eiichiro Yamamoto, Akira Takasawa, Takeshi Niinuma, Ayano Yoshido et al. Activated macrophages promote invasion by early colorectal cancer via an interleukin 1 beta ‐serum amyloid A1 axis Cancer Science 10/1/2021 [PMID: 34293235]
• Y Liu, S He, XL Wang, W Peng, QY Chen, DM Chi, JR Chen, BW Han, GW Lin, YQ Li, QY Wang, RJ Peng, PP Wei, X Guo, B Li, X Xia, HQ Mai, XD Hu, Z Zhang, YX Zeng, JX Bei Tumour heterogeneity and intercellular networks of nasopharyngeal carcinoma at single cell resolution Nature Communications, 2021-02-02;12(1):741. 2021-02-02 [PMID: 33531485] (ICC, Human)
• B Diskin, S Adam, MF Cassini, G Sanchez, M Liria, B Aykut, C Buttar, E Li, B Sundberg, RD Salas, R Chen, J Wang, M Kim, MS Farooq, S Nguy, C Fedele, KH Tang, T Chen, W Wang, M Hundeyin, JAK Rossi, E Kurz, MIU Haq, J Karlen, E Kruger, Z Sekendiz, D Wu, SAA Shadaloey, G Baptiste, G Werba, S Selvaraj, C Loomis, KK Wong, J Leinwand, G Miller PD-L1 engagement on T cells promotes self-tolerance and suppression of neighboring macrophages and effector T cells in cancer Nat. Immunol., 2020-03-09;21(4):442-454. 2020-03-09 [PMID: 32152508] (T Cell Activation, Human, Mouse)
• Melania Scarpa, Matteo Fassan, Andromachi Kotsafti, Stefano Realdon, Luigi Dall’Olmo, Tiziana Morbin et al. CD80 expression promotes immune surveillance in Barrett’s metaplasia OncoImmunology 10/3/2019 [PMID: 31646078]
• Matteo Fassan, Francesco Cavallin, Vincenza Guzzardo, Andromachi Kotsafti, Melania Scarpa, Matteo Cagol et al. PD‐L1 expression, CD8+ and CD4+ lymphocyte rate are predictive of pathological complete response after neoadjuvant chemoradiotherapy for squamous cell cancer of the thoracic esophagus Cancer Medicine 10/1/2019 [PMID: 31429521]
• Patricia Kleinpeter, Christelle Remy-Ziller, Eline Winter, Murielle Gantzer, Virginie Nourtier, Juliette Kempf et al. By Binding CD80 and CD86, the Vaccinia Virus M2 Protein Blocks Their Interactions with both CD28 and CTLA4 and Potentiates CD80 Binding to PD-L1 Journal of Virology 6/1/2019 [PMID: 30918073]
• Navleen Gill, Yaozhu Leng, Roberto Romero, Yi Xu, Bogdan Panaitescu, Derek Miller et al. The immunophenotype of decidual macrophages in acute atherosis American Journal of Reproductive Immunology 4/1/2019 [PMID: 30734977]
• S Minamikawa, K Nozu, S Maeta, T Yamamura, K Nakanishi, J Fujimura, T Horinouchi, C Nagano, N Sakakibara, H Nagase, H Shima, K Noda, T Ninchoji, H Kaito, K Iijima The utility of urinary CD80 as a diagnostic marker in patients with renal diseases Sci Rep, 2018-11-23;8(1):17322. 2018-11-23 [PMID: 30470792] (ELISA Development (Detection), Human)
• Min Zhang, Kojiro Nakamura, Shoichi Kageyama, Akeem O. Lawal, Ke Wei Gong, May Bhetraratana et al. Myeloid HO-1 modulates macrophage polarization and protects against ischemia-reperfusion injury JCI Insight 10/4/2018 [PMID: 30282830]
• J Li, SQ Xu, YM Zhao, S Yu, LH Ge, BH Xu Comparison of the biological characteristics of human mesenchymal stem cells derived from exfoliated deciduous teeth, bone marrow, gingival tissue, and umbilical cord Mol Med Rep, 2018-09-20;0(0):. 2018-09-20 [PMID: 30272340] (Flow Cytometry, Human)
• Mikio Shiba, Yasuo Sugano, Yoshihiko Ikeda, Hideshi Okada, Toshiyuki Nagai, Hatsue Ishibashi-Ueda et al. Presence of increased inflammatory infiltrates accompanied by activated dendritic cells in the left atrium in rheumatic heart disease PLOS ONE 9/27/2018 [PMID: 30261069]
• S He, C Wu, J Xiao, D Li, Z Sun, M Li Endothelial extracellular vesicles modulate the macrophage phenotype: Potential implications in atherosclerosis Scand. J. Immunol., 2018-04-01;0(0):. 2018-04-01 [PMID: 29465752] (Flow Cytometry, Human)
• SW Lee, SH Baek, JH Paik, S Kim, KY Na, DW Chae, HJ Chin Tubular B7-1 expression parallels proteinuria levels, but not clinical outcomes in adult minimal change disease patients Sci Rep, 2017-02-02;7(0):41859. 2017-02-02 [PMID: 28150736] (IHC-P, Human)
• Xiongbiao Wang Autoantibodies against CD80 in patients with COPD Clin Transl Immunology, 2016-10-07;5(10):e103. 2016-10-07 [PMID: 27867516] (Western Blot, Human)
• Elena Gagliardini, Rubina Novelli, Daniela Corna, Carlamaria Zoja, Barbara Ruggiero, Ariela Benigni et al. B7–1 Is Not Induced in Podocytes of Human and Experimental Diabetic Nephropathy Journal of the American Society of Nephrology 4/1/2016 [PMID: 26319246]
• Mao C, Mou X, Zhou Y, Yuan G, Xu C, Liu H, Zheng T, Tong J, Wang S, Chen D Tumor-activated TCRgammadelta(+) T cells from gastric cancer patients induce the antitumor immune response of TCRalphabeta(+) T cells via their antigen-presenting cell-like effects. J Immunol Res, 2014-03-31;2014(0):593562. 2014-03-31 [PMID: 24741609] (Functional Assay, Human)
• Samuel J. Bullers, Simon C. Baker, Eileen Ingham, Jennifer Southgate The human tissue-biomaterial interface: a role for PPAR gamma -dependent glucocorticoid receptor activation in regulating the CD163+ M2 macrophage phenotype Tissue Engineering Part A 9/1/2014 [PMID: 24548290]
• B7-CD28 costimulatory signals control the survival and proliferation of murine and human gammadelta T cells via IL-2 production. J. Immunol., 2012-06-25;189(3):1202-8. 2012-06-25 [PMID: 22732586] (Neutralization, Human)
• ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation. Blood, 2012-05-10;119(26):6268-77. 2012-05-10 [PMID: 22577174] (Neutralization, Human)
• Kosaka K, Yashiro M, Sakate Y, Hirakawa K A synergistic antitumor effect of interleukin-2 addition with CD80 immunogene therapy for peritoneal metastasis of gastric carcinoma. Dig. Dis. Sci., 2007-04-03;52(8):1946-53. 2007-04-03 [PMID: 17404853] (Neutralization, Human)

Bioinformatics

• Gene Symbol: CD80
• Uniprot:
  • P33681()