CTLA-4 Antibody (BNI3)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, IHC-Fr, IP
Clone
BNI3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

CTLA-4 Antibody (BNI3) Summary

Immunogen
Human CTLA-4/human IgG heavy chain fusion protein
Specificity
NB100-64849 recognizes the human CD152 cell surface antigen, also known as CTLA-4 (cytotoxic T-lymphocyte-associated antigen 4). CD152 is a 45kD glycoprotein, expressed normally as a disulphide-linked homodimer. It is expressed transiently on the surface of T cells following activation (peaking at 3 days), but is often present with the cytoplasm. CD152 is a ligand for CD80 and CD86, and is important in cellular interactions during the immune response.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CTLA4
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry Neat - 1:10
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 5 ug/mL - 50 ug/mL
  • Immunoprecipitation 1:10 - 1:500
Application Notes
Membrane permeabilization is required for Flow Cytometry. The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended.
Control
CTLA-4 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB100-64849 in the following applications:

Publications
Read Publication using
NB100-64849 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for CTLA-4 Antibody (BNI3)

  • CD
  • CD152 antigen
  • CD152
  • CD152IDDM12
  • CD28
  • celiac disease 3
  • CELIAC3
  • CTLA4
  • CTLA-4
  • cytotoxic T-lymphocyte antigen 4
  • cytotoxic T-lymphocyte protein 4
  • Cytotoxic T-lymphocyte-associated antigen 4
  • cytotoxic T-lymphocyte-associated protein 4
  • cytotoxic T-lymphocyte-associated serine esterase-4
  • GRD4
  • GSE
  • ICOS
  • ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4

Background

CTLA4 has a critical role in T cell differentiation and contributes to the maintenance of peripheral T cell tolerance to self antigens. It exerts its major effects on T cell immune responses via regulation of the cell cycle and may be involved in T cell activation. It binds to B7-1 (CD80) and B7-2 (CD86), and it contains one immunoglobulin-like V type domain. It is widely expressed, with highest levels in lymphoid tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CTLA-4 Antibody (NB100-64849)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC.


Filter By Application
IHC
(1)
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Filter By Species
Human
(1)
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Review for CTLA-4 Antibody (NB100-64849) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-64849:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC-P Human 11/06/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedhuman tonsil
SpeciesHuman
Lot030914

Product General Protocols

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FAQs for CTLA-4 Antibody (NB100-64849) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Blogs on CTLA-4.

Thomson Reuters Predicts 2016 Nobel Prize Winners
Here at Bio-Techne we always look forward to the annual announcements of winners of the highly coveted Nobel Prize – the greatest award in science. How can you go about predicting which scientists might be in line for a life-changing phone-call fro...  Read full blog post.

CD86 - I work in tandem with CD80
CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated...  Read full blog post.

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Recent Reviews

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1
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Anonymous
11/06/2015
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol CTLA4
Entrez
Uniprot