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CTLA-4 Antibody (37B3D8.2F2)

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Western Blot: CTLA-4 Antibody (37B3D8.2F2) [NBP2-31143] - Analysis of CTLA-4 protein on (A) mouse Kidney lysate (B) human Kidney lysate and on (C) partial recombinant protein using CTLA-4 monoclonal antibody at a concentration of 2 ug/ml.
Western Blot: CTLA-4 Antibody (37B3D8.2F2) [NBP2-31143] - Analysis of CTLA-4 protein on (A) mouse Kidney lysate (B) human Kidney lysate and on (C) partial recombinant protein using CTLA-4 monoclonal antibody at a ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clone
37B3D8.2F2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-31143AF350
Alexa Fluor 405
NBP2-31143AF405
Alexa Fluor 488
NBP2-31143AF488
Alexa Fluor 532
NBP2-31143AF532
Alexa Fluor 594
NBP2-31143AF594
Alexa Fluor 647
NBP2-31143AF647
Alexa Fluor 700
NBP2-31143AF700
Alexa Fluor 750
NBP2-31143AF750
Biotin
NBP2-31143B
DyLight 350
NBP2-31143UV
DyLight 405
NBP2-31143V
DyLight 488
NBP2-31143G
DyLight 550
NBP2-31143R
DyLight 594
NBP2-31143DL594
DyLight 650
NBP2-31143C
DyLight 680
NBP2-31143FR
DyLight 755
NBP2-31143IR
HRP
NBP2-31143H
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-80683
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CTLA-4 Antibody (37B3D8.2F2) Summary

Immunogen
Mouse CTLA4
Clonality
Monoclonal
Host
Mouse
Gene
CTLA4
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
Application Notes
CTLA4 protein is 223 amino acids long (~24.7 kDa) which is processed via cleavage at AA35 to produce 188 amino acids long chain which further undergoes post-translational modifications such as N-glycosylation, disulfide bond formation/dimerization and phosphorylation. In Western blot, the mature CTLA4 protein runs between ~35-45kDa position.
Control
CTLA-4 Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for CTLA-4 Antibody (37B3D8.2F2)

  • CD
  • CD152 antigen
  • CD152
  • CD152IDDM12
  • CD28
  • celiac disease 3
  • CELIAC3
  • CTLA4
  • CTLA-4
  • cytotoxic T-lymphocyte antigen 4
  • cytotoxic T-lymphocyte protein 4
  • Cytotoxic T-lymphocyte-associated antigen 4
  • cytotoxic T-lymphocyte-associated protein 4
  • cytotoxic T-lymphocyte-associated serine esterase-4
  • GRD4
  • GSE
  • ICOS
  • ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4

Background

The lymphocyte surface antigen CD152, also known as CTLA-4, is related to the co-stimulatory molecule CD28, and both molecules share common B7 family counter-receptors. (1-4) However CD152 is thought to be a negative regulator of T cell activation (1-3) and may play a role in apoptotic control of T cells. CD152 exists as a 69 kDa homodimer1, and is relatively conserved among humans, mice and chickens. (2)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: B7-1/CD80 Antibody (62N3G8) [NBP2-25255] - Human thymus stained with CD80 antibody at 5 ug/ml using peroxidase-conjugate and DAB chromogen. Note membrane and cytoplasmic staining.
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Knockout Validated: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Western blot shows lysates of Ramos human Burkitt's lymphoma parental cell line and B7-2 knockout (KO) Ramos cell line. PVDF membrane was probed with 1.0 ug/mL of Mouse Anti-Human B7-2 Monoclonal Antibody (Catalog # NBP2-25208) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for B7-2 at approximately 90 kDa (as indicated) in the parental Ramos cell line, but is not detectable in the knockout Ramos cell line. This experiment was conducted under reducing conditions.
Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Immunofluorescence staining of PFA-fixed Ramos cells using B7-2/CD86 Antibody (BU63) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.
Immunohistochemistry-Paraffin: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Formalin-fixed, paraffin-embedded esophagus tumor tissue stained with CD86 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Note strong staining of differentiated squamous cells. TMA was used for this test.
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Mouse CD3+ T Cell,
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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for CTLA-4 Antibody (NBP2-31143). (Showing 1 - 1 of 1 FAQ).

  1. Could you let us know the buffer formulation?
    • This antibody (NBP2-31143) is provided in Tris-glycine buffer with 0.05% sodium azide.

Control Lysate(s)

CTLA-4 Knockout HeLa Cell Lysate

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-31143AF350
Alexa Fluor 405 NBP2-31143AF405
Alexa Fluor 488 NBP2-31143AF488
Alexa Fluor 532 NBP2-31143AF532
Alexa Fluor 594 NBP2-31143AF594
Alexa Fluor 647 NBP2-31143AF647
Alexa Fluor 700 NBP2-31143AF700
Alexa Fluor 750 NBP2-31143AF750
Biotin NBP2-31143B
DyLight 350 NBP2-31143UV
DyLight 405 NBP2-31143V
DyLight 488 NBP2-31143G
DyLight 550 NBP2-31143R
DyLight 594 NBP2-31143DL594
DyLight 650 NBP2-31143C
DyLight 680 NBP2-31143FR
DyLight 755 NBP2-31143IR
HRP NBP2-31143H
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol CTLA4