Complement C3 Antibody

Images

 
Western Blot: Complement C3 Antibody [NBP1-32080] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with C3 antibody diluted at a dilution of 1:1000.
Immunocytochemistry/ Immunofluorescence: Complement C3 Antibody [NBP1-32080] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C3 protein stained by C3 antibody [C3], C-term diluted at 1:200. ...read more
Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-32080] - Paraffin-embedded mouse brain diluted at 1:500.
Western Blot: Complement C3 Antibody [NBP1-32080] - A. 293T whole cell lysate/extract. B. 30 ug whole cell lysate/extract of human C3-transfected 293T cells
Western Blot: Complement C3 Antibody [NBP1-32080] - Lane A: Non-transfected 293T lysates, Lane B: C3 transfected 293T lysates with diluted at 1:5000.
Western Blot: Complement C3 Antibody [NBP1-32080] - A:Hep G2.
Western Blot: Complement C3 Antibody [NBP1-32080] - A: Non-transfected 293T lysates B: C3 transfected 293T lysate.
Immunocytochemistry/ Immunofluorescence: Complement C3 Antibody [NBP1-32080] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-32080] - Paraffin-embedded mouse brain, using antibody at 1:500 dilution.
Simple Western: Complement C3 Antibody [NBP1-32080] - Simple Western lane view shows a specific band for Complement C3 in 0.05 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the ...read more
Immunoprecipitation: Complement C3 Antibody [NBP1-32080] - Immunoprecipitation of C3 protein from HepG2 whole cell extracts using 5 ug of C3 antibody [C3], C-term.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Concentration
0.16 mg/ml

Order Details

Complement C3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human C3. The exact sequence is proprietary.
Localization
Secreted
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
C3
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
0.16 mg/ml
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Simple Western 1:50
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Application Notes
Immunosubtraction.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
187 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Alternate Names for Complement C3 Antibody

  • AHUS5
  • ARMD9
  • ASP
  • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
  • complement C3
  • complement component 3
  • complement component C3
  • CPAMD1acylation-stimulating protein cleavage product

Background

Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. People with C3 deficiency are susceptable to bacteria infection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Complement C3 Antibody (NBP1-32080). (Showing 1 - 2 of 2 FAQ).

  1. I am trying to establish a method to measure mice C3 levels by nephelometry. I would be most grateful if you could provide me with some help, regarding the choice of the Ab. I totally understand that since such a method has never been tried, I do not expect any guaranties. 
    • We have never performed nephelometry in our lab, and do not have a protocol or advice to provide about this application. However, it seems to me that you should choose an antibody that is capable of recognizing its target in its folded conformation. Therefore, I would suggest trying an antibody that has been validated for ICC or IHC.
  2. At what molecular weight can we find the band when run on western blotting with and with out reducing agent?
    • The theoretical molecular weight of Complement C3 is 187kDa. With NBP1-32080, we typically see bands around 180kDa when testing in Western blot under reducing conditions. As we have not tested this antibody in non-reducing conditions we cannot say with certainty at what size you will see the band, but it will most likely be at a higher weight than the band in reducing conditions.

Secondary Antibodies

 

Isotype Controls

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Blogs on Complement C3.

Complement C3 - the most important protein in the complement system
The complement system is made up of a collection of proteins found in the bloodstream and is comprised of nine major complement proteins; complement C3 is one of them. The complement system is a crucial component of the cellular immune system becau...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol C3
Entrez
Uniprot

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