PSMA7 Antibody (1A10-3G12)

Western Blot: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Analysis of PSMA7 expression in transfected 293T cell line by PSMA7 monoclonal antibody (M01), clone 1A10-3G12.Lane 1: PSMA7 transfected lysate (Predicted MW: 27.9 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Analysis of monoclonal antibody to PSMA7 on HeLa cell. Antibody concentration 1 ~ 10 ug/ml.

Immunohistochemistry-Paraffin: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Analysis of monoclonal antibody to PSMA7 on formalin-fixed paraffin-embedded human colon tissue. Antibody concentration 5 ug/ml.

Western Blot: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - PSMA7 monoclonal antibody (M01), clone 1A10-3G12 Analysis of PSMA7 expression in HeLa.

Western Blot: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - PSMA7 monoclonal antibody (M01), clone 1A10-3G12. Analysis of PSMA7 expression in human omentum, serous carcinoma.

Immunohistochemistry-Paraffin: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Analysis of monoclonal antibody to PSMA7 on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. Antibody concentration 5 ug/ml.

Immunoprecipitation: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Analysis of PSMA7 transfected lysate using anti-PSMA7 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PSMA7 MaxPab rabbit polyclonal antibody.

Sandwich ELISA: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Detection limit for recombinant GST tagged PSMA7 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, S-ELISA
• Clone: 1A10-3G12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

PSMA7 Antibody (1A10-3G12) Summary

• Immunogen: PSMA7 (AAH04427, 1 a.a. ~ 248 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSYDRAITVFSPDGHLFQVEYAQEAVKKGSTAVGVRGRDIVVLGVEKKSVAKLQDERTVRKICALDDNVCMAFAGLTADARIVINRARVECQSHRLTVEDPVTVEYITRYIASLKQRYTQSNGRRPFGISALIVGFDFDGTPRLYQTDPSGTYHAWKANAIGRGAKSVREFLEKNYTDEAIETDDLTIKLVIKALLEVVQSGGKNIELAVMRRDQSLKILNPEEIEKYVAEIEKEKEENEKKKQKKAS
• Specificity: PSMA7 - proteasome (prosome, macropain) subunit, alpha type, 7
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PSMA7
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recominant protein, tissue lysate and cell lysate for WB. It has also been used for IF, IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PSMA7 Antibody (1A10-3G12)

• C6
• EC 3.4.25.1
• HSPC
• HSPCMGC3755
• proteasome (prosome, macropain) subunit, alpha type, 7
• proteasome subunit alpha 4
• proteasome subunit alpha type-7
• Proteasome subunit RC6-1
• Proteasome subunit XAPC7
• PSMA7
• RC6-1
• XAPC7

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. This particular subunit has been shown to interact specifically with the hepatitis B virus X protein, a protein critical to viral replication. In addition, this subunit is involved in regulating hepatitis virus C internal ribosome entry site (IRES) activity, an activity essential for viral replication. This core alpha subunit is also involved in regulating the hypoxia-inducible factor-1alpha, a transcription factor important for cellular responses to oxygen tension. Multiple isoforms of this subunit arising from alternative splicing may exist but alternative transcripts for only two isoforms have been defined. A pseudogene has been identified on chromosome 9.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PSMA7 Antibody (H00005688-M01)(2)

Publications using H00005688-M01

• Tan JY, Huang X, Luo YL. PSMA7 inhibits the tumorigenicity of A549 human lung adenocarcinoma cells. Mol Cell Biochem. 2012-05-15 [PMID: 22584585]
• Smith L, Qutob O, Watson MB et al. Proteomic identification of putative biomarkers of radiotherapy resistance: a possible role for the 26S proteasome? Neoplasia. 2009-11-01 [PMID: 19881955]

Review for PSMA7 Antibody (H00005688-M01) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Weibo Luo WB Human 01/24/2012

Summary

• Application: Western Blot
• Sample Tested: HeLa cell lysates
• Species: Human

Bioinformatics

• Gene Symbol: PSMA7
• Entrez:
  • 5688(Human)
• OMIM:
  • 606607(Human)
• Uniprot:
  • AAH04427(Human)