Complement Factor H Antibody [Unconjugated]

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Complement Factor H was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Complement Factor H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4779) at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

Complement Factor H Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Complement Factor H
Ser860-Arg1231
Accession # P08603
Specificity
Detects human Complement Factor H in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant mouse Complement Factor H is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CFH
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF4779 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Complement Factor H Antibody [Unconjugated]

  • adrenomedullin binding protein
  • age-related maculopathy susceptibility 1
  • AHUS1
  • AMBP1
  • ARMD4
  • ARMS1
  • beta-1H
  • beta-1-H-globulin
  • beta-1-H-globulin
  • CFH
  • CFHL3
  • Complement Factor H
  • factor H
  • factor H-like 1
  • FH
  • FHL1
  • H factor 1 (complement)
  • H factor 1
  • H factor 2 (complement)
  • HF
  • HF1
  • HF1ARMS1
  • HF2
  • HUS
  • HUSMGC88246

Background

Complement Factor H is a 155 kDa glycoprotein that provides critical negative regulation to the alternative pathway of complement cascade. It is secreted by Kupffer cells, hepatocytes, vascular endothelial cells, and platelets, and circulates in the serum at high concentration (1). Complement Factor H is composed of 20 SCRs (short consensus repeats), each of which consists of approximately 60 amino acids with four invariant Cys residues (2). Alternate splicing generates an isoform that is truncated following SCR7. Complement Factor H interacts with cell surface polyanions including heparin and sialoglycoproteins (3-6), and immobilized Complement Factor H supports the CD11b/CD18 integrin-dependent adhesion of neutrophils (7). It prevents local complement activation by sequestering complement component C3b, accelerating the decay of C3 and C5 convertases, and functions as a cofactor for the C3b inactivator, Factor I (1, 3, 6, 8). The recombinant protein expressed here corresponds to SCR15-20, which encompass the primary binding sites for heparin and C3b, as well as for the peptide hormone adrenomedullin (4, 9‑11). Within SCR15-20, human Complement Factor H shares 60% and 63% amino acid sequence identity with mouse and rat Complement Factor H, respectively. Dozens of mutations clustered in SCR15-20 are associated with atypical hemolytic uremic syndrome, a disorder characterized by anemia, thrombocytopenia, and renal failure (12). Binding of Complement Factor H to tumor cell-associated dentin matrix protein 1, bone sialoprotein, or osteopontin results in the protection of that cell from complement-mediated lysis (13, 14). A variety of pathogenic microbes also express Complement Factor H binding molecules that interfere with immune clearance of the infection (15).

  1. Schmidt, C.Q. et al. (2008) Clin. Exp. Immunol. 151:14. 
  2. Ripoche, J. et al. (1988) Biochem. J. 249:593. 
  3. Meri, S. and M.K. Pangburn (1990) Proc. Natl. Acad. Sci. USA 87:3982. 
  4. Jokiranta, T.S. et al. (2005) Am. J. Pathol. 167:1173. 
  5. Blackmore, T.K. et al. (1998) J. Immunol. 160:3342. 
  6. Hellwage, J. et al. (2002) J. Immunol. 169:6935. 
  7. DiScipio, R.G. et al. (1998) J. Immunol. 160:4057. 
  8. Sharma, A.K. and M.K. Pangburn (1996) Proc. Natl. Acad. Sci. USA 93:10996.
  9. Oppermann, M. et al. (2006) Clin. Exp. Immunol. 144:342.
  10. Pangburn, M.K. et al. (2000) J. Immunol. 164:4742.
  11. Martinez, A. et al. (2003) Hypertens. Res. 26:S55.
  12. de Cordoba, S.R. and E.G. de Jorge (2008) Clin. Exp. Immunol. 151:1.
  13. Jain, A. et al. (2002) J. Biol. Chem. 277:13700.
  14. Fedarko, N.S. et al. (2000) J. Biol. Chem. 275:16666.
  15. Kraiczy, P. and R. Wurzner (2006) Mol. Immunol. 43:31.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Complement Factor H Antibody (AF4779) (0)

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Review for Complement Factor H Antibody (AF4779) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF4779:
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Simple Western
(1)
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Images Ratings Applications Species Date Details
Simple Western Complement Factor H AF4779
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reviewed by:
Anonymous
Simple Western Human 09/25/2019
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Summary

ApplicationSimple Western
Sample TestedHuman serum,Purified protein
SpeciesHuman
LotCBDG0218021

Comments

CommentsInput samples were dilution series of human serum pool or purified complement factor H. The AF4779 antibody was tested at three different concentrations; 10, 5 and 1 ug/ml (left to right).
Samples were analysed by Simple Western under reducing conditions and the 12-230 kDa separation module.

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Anonymous
09/25/2019
Application: Simple Western
Species: Human

Bioinformatics

Gene Symbol CFH
Uniprot
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