Recombinant Mouse Complement Component C5a Protein

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1 μg/lane of Recombinant Mouse Complement Component C5a was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 9 kDa.
Recombinant Mouse Complement Component C5a (Catalog # 2150-C5) induces N-acetyl-beta -D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. The ED50 for this effect is 5-20 ng/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Catalog# & Formulation Size Price

Recombinant Mouse Complement Component C5a Protein Summary

Details of Functionality
Measured by its ability to induce N-acetyl-beta -D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. Gerard, NP and Gerard, C. (1990) Biochemistry 29:9274. The ED50 for this effect is 5-20 ng/mL.
Source
E. coli-derived mouse Complement Component C5a protein
Asn679-Arg755, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
Hc
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
9.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
2150-C5 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Complement Component C5a Protein

  • C5
  • C5A
  • Complement Component C5a
  • HE

Background

Mouse Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as Anaphylatoxins. C5a is a 77 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain (1, 2). Mouse C5a has four alpha -helices, plus three intrachain disulfide bonds that create a triple loop structure (3). In serum, proteolytic processing removes the C-terminal arginine, creating a low activity C5a desArg77 molecule (1). Mouse C5a shares 60% and 82% aa sequence identity to human and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (GPCR) (C5aR/CD88), inducing neutrophil chemotaxis and endothelial cell activation (1, 4). It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells (1, 4). Alternatively, it may also bind to a nonsignaling GPCR termed C5L2 whose function is yet to be determined (5).

  1. Gerard, C. and N.P. Gerard (1994) Annu. Rev. Immunol. 12:775.
  2. DiScipio, R.G. et al. (1983) J. Biol. Chem. 258:10629.
  3. Huber-Lang, M.S. et al. (2003) J. Immunol. 170:6115.
  4. Gerard, N.P. and C. Gerard (2002) Curr. Opin. Immunol. 14:705.
  5. Okinaga, S. et al. (2003) Biochemistry 42:9406.

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Publications for Complement Component C5a (2150-C5)(38)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 6 applications: Bioassay, Cell Culture, ELISA (Standard), In Vivo, Surface Plasmon Resonance, Western Blot.


Filter By Application
Bioassay
(27)
Cell Culture
(1)
ELISA (Standard)
(2)
In Vivo
(4)
Surface Plasmon Resonance
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(33)
N/A
(2)
All Species
Showing Publications 1 - 10 of 38. Show All 38 Publications.
Publications using 2150-C5 Applications Species
W Xiong, L Jia, J Liang, Y Cai, Y Chen, Y Nie, J Jin, J Zhu Investigation into the anti-airway inflammatory role of the PI3Kgamma inhibitor JN-PK1: An in vitro and in vivo study International immunopharmacology, 2022;111(0):109102. 2022 [PMID: 35964410] (Cell Culture, Mouse) Cell Culture Mouse
M Llorián-Sa, EM Byrne, M Szczepan, K Little, M Chen, H Xu Complement activation contributes to subretinal fibrosis through the induction of epithelial-to-mesenchymal transition (EMT) in retinal pigment epithelial cells Journal of Neuroinflammation, 2022;19(1):182. 2022 [PMID: 35831910] (Bioassay, Mouse) Bioassay Mouse
L Brandolini, M d'Angelo, R Novelli, V Castelli, C Giorgio, A Sirico, P Cocchiaro, F D'Egidio, E Benedetti, C Cristiano, A Bugatti, A Ruocco, PG Amendola, C Talarico, C Manelfi, D Iaconis, A Beccari, AU Quadros, TM Cunha, A Caruso, R Russo, A Cimini, A Aramini, M Allegretti Paclitaxel binds and activates C5aR1: A new potential therapeutic target for the prevention of chemotherapy-induced peripheral neuropathy and hypersensitivity reactions Cell Death & Disease, 2022;13(5):500. 2022 [PMID: 35614037] (Bioassay, Mouse) Bioassay Mouse
FM Consonni, A Bleve, MG Totaro, M Storto, P Kunderfran, A Termanini, F Pasqualini, C Alì, C Pandolfo, F Sgambellur, G Grazia, M Santinami, A Maurichi, M Milione, M Erreni, A Doni, M Fabbri, L Gribaldo, E Rulli, MP Soares, V Torri, R Mortarini, A Anichini, A Sica Heme catabolism by tumor-associated macrophages controls metastasis formation Nature Immunology, 2021;22(5):595-606. 2021 [PMID: 33903766] (Bioassay, Mouse) Bioassay Mouse
E van den Bo, B Ambrosy, M Horsthemke, S Walbaum, AC Bachg, N Wettschure, G Innamorati, TM Wilkie, PJ Hanley Knockout mouse models reveal the contributions of G protein subunits to complement C5a receptor-mediated chemotaxis J. Biol. Chem., 2020;0(0):. 2020 [PMID: 32332099] (Bioassay, Mouse) Bioassay Mouse
AB Schmider, NC Bauer, H Sunwoo, MD Godin, GE Ellis, JT Lee, PA Nigrovic, RJ Soberman Two and three-color STORM analysis reveals higher-order assembly of leukotriene synthetic complexes on the nuclear envelope of murine neutrophils J. Biol. Chem., 2020;0(0):. 2020 [PMID: 32152223] (Bioassay, Mouse) Bioassay Mouse
BE Hsu, J Roy, J Mouhanna, RF Rayes, L Ramsay, S Tabariès, MG Annis, IR Watson, JD Spicer, S Costantino, PM Siegel C3a elicits unique migratory responses in immature low-density neutrophils Oncogene, 2020;0(0):. 2020 [PMID: 32020055] (Bioassay, Mouse) Bioassay Mouse
C Muth, A Hartmann, D Sepulveda-, M Glatzel, S Krasemann Phagocytosis of Apoptotic Cells Is Specifically Upregulated in ApoE4 Expressing Microglia in vitro Front Cell Neurosci, 2019;13(0):181. 2019 [PMID: 31130847] (Bioassay, Mouse) Bioassay Mouse
K Kobayakawa, Y Ohkawa, S Yoshizaki, T Tamaru, T Saito, K Kijima, K Yokota, M Hara, K Kubota, Y Matsumoto, K Harimaya, K Ozato, T Masuda, M Tsuda, T Tamura, K Inoue, VR Edgerton, Y Iwamoto, Y Nakashima, S Okada Macrophage centripetal migration drives spontaneous healing process after spinal cord injury Sci Adv, 2019;5(5):eaav5086. 2019 [PMID: 31106270] (In Vivo, Mouse) In Vivo Mouse
N Funk, M Munz, T Ott, K Brockmann, A Wenninger-, R Kühn, D Vogt-Weise, F Giesert, W Wurst, T Gasser, S Biskup The Parkinson&#039;s disease-linked Leucine-rich repeat kinase 2 (LRRK2) is required for insulin-stimulated translocation of GLUT4 Sci Rep, 2019;9(1):4515. 2019 [PMID: 30872638] (Bioassay, Mouse) Bioassay Mouse
Show All 38 Publications.

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Bioinformatics

Gene Symbol Hc
Uniprot