Recombinant Mouse Complement Component C5a Protein

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1 μg/lane of Recombinant Mouse Complement Component C5a was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 9 kDa.
Recombinant Mouse Complement Component C5a (Catalog # 2150-C5) induces N-acetyl-beta -D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. The ED50 for this effect is 5-20 ng/mL.

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Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse Complement Component C5a Protein Summary

Details of Functionality
Measured by its ability to induce N-acetyl-beta -D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. Gerard, NP and Gerard, C. (1990) Biochemistry 29:9274. The ED50 for this effect is 5-20 ng/mL.
Source
E. coli-derived mouse Complement Component C5a protein
Asn679-Arg755, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
Hc
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
9.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
2150-C5 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Complement Component C5a Protein

  • C5
  • C5A
  • Complement Component C5a
  • HE

Background

Mouse Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as Anaphylatoxins. C5a is a 77 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain (1, 2). Mouse C5a has four alpha -helices, plus three intrachain disulfide bonds that create a triple loop structure (3). In serum, proteolytic processing removes the C-terminal arginine, creating a low activity C5a desArg77 molecule (1). Mouse C5a shares 60% and 82% aa sequence identity to human and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (GPCR) (C5aR/CD88), inducing neutrophil chemotaxis and endothelial cell activation (1, 4). It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells (1, 4). Alternatively, it may also bind to a nonsignaling GPCR termed C5L2 whose function is yet to be determined (5).

  1. Gerard, C. and N.P. Gerard (1994) Annu. Rev. Immunol. 12:775.
  2. DiScipio, R.G. et al. (1983) J. Biol. Chem. 258:10629.
  3. Huber-Lang, M.S. et al. (2003) J. Immunol. 170:6115.
  4. Gerard, N.P. and C. Gerard (2002) Curr. Opin. Immunol. 14:705.
  5. Okinaga, S. et al. (2003) Biochemistry 42:9406.

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Publications for Complement Component C5a (2150-C5)(29)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 5 applications: Bioassay, ELISA (Standard), In Vivo, Surface Plasmon Resonance, Western Blot.


Filter By Application
Bioassay
(20)
ELISA (Standard)
(2)
In Vivo
(3)
Surface Plasmon Resonance
(1)
Western Blot
(1)
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Human
(1)
Mouse
(24)
N/A
(2)
All Species
Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using 2150-C5 Applications Species
N Funk, M Munz, T Ott, K Brockmann, A Wenninger-, R Kühn, D Vogt-Weise, F Giesert, W Wurst, T Gasser, S Biskup The Parkinson&#039;s disease-linked Leucine-rich repeat kinase 2 (LRRK2) is required for insulin-stimulated translocation of GLUT4 Sci Rep, 2019;9(1):4515. 2019 [PMID: 30872638] (Bioassay, Mouse) Bioassay Mouse
S Arandjelov, JSA Perry, CD Lucas, KK Penberthy, TH Kim, M Zhou, DA Rosen, TY Chuang, AM Bettina, LS Shankman, AH Cohen, A Gaultier, TP Conrads, M Kim, MR Elliott, KS Ravichandr A noncanonical role for the engulfment gene ELMO1 in neutrophils that promotes inflammatory arthritis Nat. Immunol., 2019;20(2):141-151. 2019 [PMID: 30643265] (Bioassay, Mouse) Bioassay Mouse
MA Crowley, O Delgado, A Will-Orreg, NM Buchanan, K Anderson, BD Jaffee, TP Dryja, SM Liao Induction of Ocular Complement Activation by Inflammatory Stimuli and Intraocular Inhibition of Complement Factor D in Animal Models Invest. Ophthalmol. Vis. Sci., 2018;59(2):940-951. 2018 [PMID: 29450541] (Bioassay, Mouse) Bioassay Mouse
CB Toomey, M Landowski, M Klingeborn, U Kelly, J Deans, H Dong, O Harrabi, T Van Blarco, YA Yeung, R Grishanin, JC Lin, DR Saban, C Bowes Rick Effect of Anti-C5a Therapy in a Murine Model of Early/Intermediate Dry Age-Related Macular Degeneration Invest. Ophthalmol. Vis. Sci., 2018;59(2):662-673. 2018 [PMID: 29392311] (Mouse) Mouse
JB Herrmann, M Muensterma, L Strobel, A Schubert-U, TM Woodruff, SD Gray-Owen, A Klos, KO Johswich Complement C5a Receptor 1 Exacerbates the Pathophysiology of N.�meningitidis Sepsis and Is a Potential Target for Disease Treatment MBio, 2018;9(1):. 2018 [PMID: 29362231] (ELISA (Standard), Mouse) ELISA (Standard) Mouse
SN Syed, E Rau, M Ziegelmann, G Sogkas, B Brüne, RE Schmidt C5aR activation in the absence of C5a - a new disease mechanism of Autoimmune Hemolytic Anemia in mice Eur. J. Immunol., 2017;0(0):. 2017 [PMID: 29277896] (Bioassay) Bioassay
Y Wang, P Lai, X Chen, C He, X Huang, S Geng, C Luo, S Wu, W Ling, L Zhong, Z Lu, P Li, J Weng, X Du Attenuation of cGVHD by C5a/C5aR blockade is associated with increased frequency of Treg Sci Rep, 2017;7(1):3603. 2017 [PMID: 28620195] (Bioassay, Mouse) Bioassay Mouse
S Bergdolt, A Kovtun, Y Hägele, A Liedert, T Schinke, M Amling, M Huber-Lang, A Ignatius Osteoblast-specific overexpression of complement receptor C5aR1 impairs fracture healing PLoS ONE, 2017;12(6):e0179512. 2017 [PMID: 28614388] (Bioassay, Mouse) Bioassay Mouse
MM Markiewski, SK Vadrevu, SK Sharma, NK Chintala, S Ghouse, JH Cho, DP Fairlie, Y Paterson, A Astrinidis, M Karbownicz The Ribosomal Protein S19 Suppresses Antitumor Immune Responses via the Complement C5a Receptor 1 J. Immunol., 2017;198(7):2989-2999. 2017 [PMID: 28228558] (Bioassay, Mouse) Bioassay Mouse
The Complement System Component C5a Produces Thermal Hyperalgesia via Macrophage-to-Nociceptor Signaling That Requires NGF and TRPV1 J Neurosci, 2016;36(18):5055-70. 2016 [PMID: 27147658] (In Vivo, Mouse) In Vivo Mouse
Show All 29 Publications.

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Bioinformatics

Gene Symbol Hc
Uniprot