Recombinant Mouse Complement Component C5a Protein

Images

 
1 μg/lane of Recombinant Mouse Complement Component C5a was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 9 kDa.
Recombinant Mouse Complement Component C5a (Catalog # 2150-C5) induces N-acetyl-beta -D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. The ED50 for this effect is 5-20 ng/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Mouse Complement Component C5a Protein Summary

Details of Functionality
Measured by its ability to induce N-acetyl-beta -D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. Gerard, NP and Gerard, C. (1990) Biochemistry 29:9274. The ED50 for this effect is 5-20 ng/mL.
Source
E. coli-derived mouse Complement Component C5a protein
Asn679-Arg755, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
Hc
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
9.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
2150-C5 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Complement Component C5a Protein

  • C5
  • C5A
  • Complement Component C5a
  • HE

Background

Mouse Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as Anaphylatoxins. C5a is a 77 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain (1, 2). Mouse C5a has four alpha -helices, plus three intrachain disulfide bonds that create a triple loop structure (3). In serum, proteolytic processing removes the C-terminal arginine, creating a low activity C5a desArg77 molecule (1). Mouse C5a shares 60% and 82% aa sequence identity to human and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (GPCR) (C5aR/CD88), inducing neutrophil chemotaxis and endothelial cell activation (1, 4). It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells (1, 4). Alternatively, it may also bind to a nonsignaling GPCR termed C5L2 whose function is yet to be determined (5).

  1. Gerard, C. and N.P. Gerard (1994) Annu. Rev. Immunol. 12:775.
  2. DiScipio, R.G. et al. (1983) J. Biol. Chem. 258:10629.
  3. Huber-Lang, M.S. et al. (2003) J. Immunol. 170:6115.
  4. Gerard, N.P. and C. Gerard (2002) Curr. Opin. Immunol. 14:705.
  5. Okinaga, S. et al. (2003) Biochemistry 42:9406.

Customers Who Viewed This Item Also Viewed...

NB200-540
Species: Mu
Applications: Flow, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB120-11899
Species: Mu
Applications: WB, IHC, IHC-Fr
H00005688-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, S-ELISA
NBP2-67656
Species: Hu, Mu, Rt, Ze
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
MAB3648
Species: Hu
Applications: Flow, CyTOF-ready, Neut
NBP2-80406
Species: Hu
Applications: WB, Flow, ICC/IF, IHC-P
NBP1-88527
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-93935
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC
NBP2-15952
Species: Hu, Mu
Applications: WB, ICC/IF
MAB6529
Species: Hu
Applications: WB, Simple Western
NBP2-98988
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-89985
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
AF4779
Species: Hu
Applications: WB, IHC
NBP1-87492
Species: Hu
Applications: WB, IHC, IHC-P
2150-C5
Species: Mu
Applications: Bioactivity

Publications for Complement Component C5a (2150-C5)(31)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 5 applications: Bioassay, ELISA (Standard), In Vivo, Surface Plasmon Resonance, Western Blot.


Filter By Application
Bioassay
(21)
ELISA (Standard)
(2)
In Vivo
(4)
Surface Plasmon Resonance
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(26)
N/A
(2)
All Species
Showing Publications 1 - 10 of 31. Show All 31 Publications.
Publications using 2150-C5 Applications Species
C Muth, A Hartmann, D Sepulveda-, M Glatzel, S Krasemann Phagocytosis of Apoptotic Cells Is Specifically Upregulated in ApoE4 Expressing Microglia in vitro Front Cell Neurosci, 2019;13(0):181. 2019 [PMID: 31130847] (Bioassay, Mouse) Bioassay Mouse
K Kobayakawa, Y Ohkawa, S Yoshizaki, T Tamaru, T Saito, K Kijima, K Yokota, M Hara, K Kubota, Y Matsumoto, K Harimaya, K Ozato, T Masuda, M Tsuda, T Tamura, K Inoue, VR Edgerton, Y Iwamoto, Y Nakashima, S Okada Macrophage centripetal migration drives spontaneous healing process after spinal cord injury Sci Adv, 2019;5(5):eaav5086. 2019 [PMID: 31106270] (In Vivo, Mouse) In Vivo Mouse
N Funk, M Munz, T Ott, K Brockmann, A Wenninger-, R Kühn, D Vogt-Weise, F Giesert, W Wurst, T Gasser, S Biskup The Parkinson&#039;s disease-linked Leucine-rich repeat kinase 2 (LRRK2) is required for insulin-stimulated translocation of GLUT4 Sci Rep, 2019;9(1):4515. 2019 [PMID: 30872638] (Bioassay, Mouse) Bioassay Mouse
S Arandjelov, JSA Perry, CD Lucas, KK Penberthy, TH Kim, M Zhou, DA Rosen, TY Chuang, AM Bettina, LS Shankman, AH Cohen, A Gaultier, TP Conrads, M Kim, MR Elliott, KS Ravichandr A noncanonical role for the engulfment gene ELMO1 in neutrophils that promotes inflammatory arthritis Nat. Immunol., 2019;20(2):141-151. 2019 [PMID: 30643265] (Bioassay, Mouse) Bioassay Mouse
MA Crowley, O Delgado, A Will-Orreg, NM Buchanan, K Anderson, BD Jaffee, TP Dryja, SM Liao Induction of Ocular Complement Activation by Inflammatory Stimuli and Intraocular Inhibition of Complement Factor D in Animal Models Invest. Ophthalmol. Vis. Sci., 2018;59(2):940-951. 2018 [PMID: 29450541] (Bioassay, Mouse) Bioassay Mouse
CB Toomey, M Landowski, M Klingeborn, U Kelly, J Deans, H Dong, O Harrabi, T Van Blarco, YA Yeung, R Grishanin, JC Lin, DR Saban, C Bowes Rick Effect of Anti-C5a Therapy in a Murine Model of Early/Intermediate Dry Age-Related Macular Degeneration Invest. Ophthalmol. Vis. Sci., 2018;59(2):662-673. 2018 [PMID: 29392311] (Mouse) Mouse
JB Herrmann, M Muensterma, L Strobel, A Schubert-U, TM Woodruff, SD Gray-Owen, A Klos, KO Johswich Complement C5a Receptor 1 Exacerbates the Pathophysiology of N.�meningitidis Sepsis and Is a Potential Target for Disease Treatment MBio, 2018;9(1):. 2018 [PMID: 29362231] (ELISA (Standard), Mouse) ELISA (Standard) Mouse
SN Syed, E Rau, M Ziegelmann, G Sogkas, B Brüne, RE Schmidt C5aR activation in the absence of C5a - a new disease mechanism of Autoimmune Hemolytic Anemia in mice Eur. J. Immunol., 2017;0(0):. 2017 [PMID: 29277896] (Bioassay) Bioassay
Y Wang, P Lai, X Chen, C He, X Huang, S Geng, C Luo, S Wu, W Ling, L Zhong, Z Lu, P Li, J Weng, X Du Attenuation of cGVHD by C5a/C5aR blockade is associated with increased frequency of Treg Sci Rep, 2017;7(1):3603. 2017 [PMID: 28620195] (Bioassay, Mouse) Bioassay Mouse
S Bergdolt, A Kovtun, Y Hägele, A Liedert, T Schinke, M Amling, M Huber-Lang, A Ignatius Osteoblast-specific overexpression of complement receptor C5aR1 impairs fracture healing PLoS ONE, 2017;12(6):e0179512. 2017 [PMID: 28614388] (Bioassay, Mouse) Bioassay Mouse
Show All 31 Publications.

Reviews for Complement Component C5a (2150-C5) (0)

There are no reviews for Complement Component C5a (2150-C5). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Complement Component C5a (2150-C5) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional Complement Component C5a Products

Bioinformatics Tool for Complement Component C5a (2150-C5)

Discover related pathways, diseases and genes to Complement Component C5a (2150-C5). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Complement Component C5a (2150-C5)

Discover more about diseases related to Complement Component C5a (2150-C5).
 

Pathways for Complement Component C5a (2150-C5)

View related products by pathway.

PTMs for Complement Component C5a (2150-C5)

Learn more about PTMs related to Complement Component C5a (2150-C5).

Blogs on Complement Component C5a

There are no specific blogs for Complement Component C5a, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse Complement Component C5a Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol Hc
Uniprot