Collagen I alpha 1 Antibody Summary
Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of 'type' specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. Type-I collagen is the predominant form of collagen in the human body and plays a role in scar tissue formation. Collagen type 1 is also an important component of tendons and found in most connective tissues including cartilage. Collagen 1 is associated with several diseases including: Ehlers Danlos syndrome (arthrochalasia), Ehlers-Danlos syndrome (classical type), Osteogenesis imperfecta type 1-4 and osteoporosis.
Collagen Type I from human and bovine placenta by limited pepsin digestion and selective salt precipitation.
Typically less than 1% cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Immunogen affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0.
0.01% Sodium Azide
Immunogen affinity purified
Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, and western blotting for highly sensitive qualitative analysis. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for in immunoblotting. ICC/IF reported in the literature (PMID: 21903125) IHC-F reported in the literature (PMID: 22402320). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
|Read Publications using
NB600-408 in the following applications:
Expected to react with most mammalian Type I collagens. Porcine reactivity reported in scientific literature (PMID: 21688145)
Collagen type I consists of alpha-1 (139 kDa) and alpha-2 chains (129kDa). Since collagen type I is a triple helix consisting of one alpha-2 chain and two alpha-1 chains, one can expect bands of the dimeric (~270 kDa) and the trimeric form (~400 kDa). Remember that those chains are cross-linked and can't be broken by typical sample denaturation for SDS-PAGE.
Alternate Names for Collagen I alpha 1 Antibody
- Alpha-1 type I collagen
- Collagen 1 alpha 1
- Collagen 1
- collagen alpha 1 chain type I
- collagen alpha-1(I) chain
- collagen of skin, tendon and bone, alpha-1 chain
- collagen, type I, alpha 1
- pro-alpha-1 collagen type 1
The pro-alpha 1 subunit of type 1 collagen is encoded by the COL1A1 gene. The collagen type 1 protein is a triple helix composed by one alpha 2 chain and two alpha 1 chains. Type 1 collagen is abundant in bone, dermis, tendon and cornea tissues and is the fibril forming collagen found in the majority of connective tissues. A type of skin tumor called dermatofibrosarcoma protuberans has been linked to reciprocal translocations between the 17th and 22nd chromosomes where the COL1A1 gene is located. Alternate polyadenylation signals result in two transcripts for the collagen type 1 gene. COL3A1 is an important paralog of this gene. Studies using the collagen I alpha 1 antibody show that COL-I degradation is required for tumor growth in certain cancer types where a MT1-MMP complex acts to destroy collagen(1). In a study conducted at the University of Minnesota the collagen I alpha 1 antibody was used to look at the efficacy of using HBOECs (human blood outgrowth endothelial cells) to form tissue-engineered vascular grafts by examining collagen deposition on bioartificial tissue that was either seeded with HBOECs or unseeded(2). Analysis of collagen structure and deposition in tissues using the collagen I alpha 1 antibody has shown that this collagen I alpha 1 antibody is a useful tool in both cancer and wound healing research areas. PMIDs: 1. 22406620 - IHC Staining of Xenografts with Collagen I alpha 1 Antibody 2. 21599543 - Collagen I Alpha 1 Antibody Used in IF Staining of ECM Protein Depositions
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
Customers Who Viewed This Item Also Viewed...
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Bv, Ma
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
Species: Hu, Rt
Applications: IHC, ICC, ICFlow
Species: Hu, Rt, Bv, Rb
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP
Applications: WB, IHC
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Fi
Applications: ELISA, ICC/IF, MiAr
Species: Hu, Rt(-)
Applications: WB, ICC/IF, IHC-Fr, IHC-P, IP
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC
Applications: WB, IHC
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for Collagen I alpha 1 Antibody (NB600-408). (Showing 1 - 10 of 10 FAQs).
We are looking for an anti-Collagen antibody for Flow Cytometry (FACS) analysis in Swine sample. Would you please provide some references if there is any available as well?
- We currently have one antibody for porcine Collagen validated in flow cytometry (catalog # NBP1-94202).
I am looking for anti collagen 1 antibody, I see your product calls it collagen I alpha 1 is there a difference?
- Collagen I alpha 1 is the most common protein for the collagen 1 protein. There are other subunits as well (such as collagen 1 alpha 2…etc.).
Can this collagen 1 alpha 1 antibody be conjugated?
- Unfortunately we do not offer this particular collagen 1 alpha 1 antibody in a directly conjugated form; however you may want to consider one of our other collagen I alpha 1 antibodies such as NBP1-77458 that is offered in several conjugated forms.
What is the epitope for this collagen I alpha 1 antibody? I need to make sure that there won’t be cross reactivity with other collagens.
- This collagen I alpha 1 antibody has not been epitope mapped; however, it has been shown there is less than 1% cross reactivity between other collagen forms and this collagen I alpha 1 antibody.
What is the immunogen for this collagen antibody?
- Pepsin digested type 1 collagen from human and bovine placenta was used as the immunogen for this collagen I alpha 1 antibody.
The predicted molecular weight says 150kDA but the band on the gel appears at 130kDA why is that?
- Differences between the predicted and observed MW can be affected by a number of factors including PTMs, relative charges and experimental factors. These cumulative effect of these differences can be quite substantial with larger molecules such as collagen I alpha 1.
How long does delivery take for your collagen 1 alpha 1 antibody?
- Our most popular collagen I alpha 1 antibody (NB600-408) is usually in stock and ships priority overnight for next business day delivery in the U.S.
What secondary antibody do you recommend for collagen I alpha 1 antibodies?
- Because this collagen I alpha 1 antibody is raised in rabbit you would want to use any anti-rabbit IgG secondary antibody. NB7160 is a popular choice for use in WB.
Could you please advise if this antibody works for IHC-P application using bone tissues?
- NB600-408 has been tested and validated for use in the following applications and species: Western blot, ELISA, IP and IHC-Paraffin in human, mouse, rat and bovine. We have not specifically tested bone tissue.
Do you follow standard Western procedures with this collagen Ab or can the protein not be denatured?
- Since we have not posted any special instruction for using this antibody in WB on our web, you could use the standard WB protocol. Although this collagen I a 1 is a huge protein (Uniprot site), the structure is relatively simple; with few modified amino acids and glycosylations. The detection of this protein with SDS-PAGE (denaturing gel) will ensure the molecular size of the protein could be accurately detected and confirmed, even though the glycosylations may alter the true MW. The manufacture of NB600-408 was carried out by the injections of rabbit with the immunogens derived from native collagen Type I fragments derived from human and bovine placenta after the limited pepsin digestions and selective salt precipitations. Therefore this antibody should recognize the native, undenatured collagen 1 alpha 1. If using the undenatured lysates in WB, you should expect to detect a very large protein complexes on your gel/WB, as the collagen I a 1 interacts with itself and/or other components in the extracellular matrices; but our WB protocol should be able to serve the propose.
Additional Collagen I alpha 1 Antibody Products
Related Products by Gene
Bioinformatics Tool for Collagen I alpha 1 Antibody (NB600-408)
Discover related pathways, diseases and genes to Collagen I alpha 1 Antibody (NB600-408). Need help?
Read the Bioinformatics Tool Guide
for instructions on using this tool.
Blogs on Collagen I alpha 1