Collagen I alpha 1 Antibody

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ELISA, ICC/IF, IHC, IHC-P, RIA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Collagen I alpha 1 Antibody Summary

Immunogen
Mouse collagen type I extracted and purified from mouse skin.
Localization
Extracellular matrix
Specificity
This is specific to Mouse Collagen Type I (100%) by RIA, and typically showed less than 1% reactivity against other types of mouse collagen types (II, III and IV).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
COL1A1
Purity
Chromatography
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Applications/Dilutions

Dilutions
  • ELISA 1:200
  • Immunocytochemistry/Immunofluorescence 1:80
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Radioimmunoassay 1:100-1:2000
  • Immunofluorescence
Theoretical MW
138 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB600-1054 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 29364500).. Cross-Reactivity (% @ RIA 1:200 Dilution): Mouse Collagen Type I 100% Mouse Collagen Type III <1.0% Mouse Collagen Type II, IV <0.1% Human, Chicken, Rat Collagen Type I <0.1%

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Deionized water
Preservative
No Preservative
Concentration
LYOPH
Purity
Chromatography
Reconstitution Instructions
Reconstitute with 0.5 mL deionized water.

Alternate Names for Collagen I alpha 1 Antibody

  • Alpha-1 type I collagen
  • COL1A1
  • Collagen 1 alpha 1
  • Collagen 1
  • collagen alpha 1 chain type I
  • collagen alpha-1(I) chain
  • Collagen I alpha 1
  • collagen of skin, tendon and bone, alpha-1 chain
  • collagen, type I, alpha 1
  • Collagen1
  • Collagen-1
  • OI4
  • pro-alpha-1 collagen type 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Collagen I alpha 1 Antibody (NB600-1054)(3)

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for Collagen I alpha 1 Antibody (NB600-1054). (Showing 1 - 8 of 8 FAQs).

  1. We are looking for an anti-Collagen antibody for Flow Cytometry (FACS) analysis in Swine sample. Would you please provide some references if there is any available as well?
    • We currently have one antibody for porcine Collagen validated in flow cytometry (catalog # NBP1-94202).
  2. I am looking for anti collagen 1 antibody, I see your product calls it collagen I alpha 1 is there a difference?
    • Collagen I alpha 1 is the most common protein for the collagen 1 protein. There are other subunits as well (such as collagen 1 alpha 2…etc.).
  3. Can this collagen 1 alpha 1 antibody be conjugated?
    • Unfortunately we do not offer this particular collagen 1 alpha 1 antibody in a directly conjugated form; however you may want to consider one of our other collagen I alpha 1 antibodies such as NBP1-77458 that is offered in several conjugated forms.
  4. What is the epitope for this collagen I alpha 1 antibody? I need to make sure that there won’t be cross reactivity with other collagens.
    • This collagen I alpha 1 antibody has not been epitope mapped; however, it has been shown there is less than 1% cross reactivity between other collagen forms and this collagen I alpha 1 antibody.
  5. What is the immunogen for this collagen antibody?
    • Pepsin digested type 1 collagen from human and bovine placenta was used as the immunogen for this collagen I alpha 1 antibody.
  6. The predicted molecular weight says 150kDA but the band on the gel appears at 130kDA why is that?
    • Differences between the predicted and observed MW can be affected by a number of factors including PTMs, relative charges and experimental factors. These cumulative effect of these differences can be quite substantial with larger molecules such as collagen I alpha 1.
  7. How long does delivery take for your collagen 1 alpha 1 antibody?
    • Our most popular collagen I alpha 1 antibody (NB600-408) is usually in stock and ships priority overnight for next business day delivery in the U.S.
  8. What secondary antibody do you recommend for collagen I alpha 1 antibodies?
    • Because this collagen I alpha 1 antibody is raised in rabbit you would want to use any anti-rabbit IgG secondary antibody. NB7160 is a popular choice for use in WB.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol COL1A1
Entrez
Uniprot