SP1 Antibody

Images

 
Biological Strategies: Western Blot: SP1 Antibody [NB600-233] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse Renca cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit ...read more
Immunohistochemistry-Paraffin: SP1 Antibody [NB600-233] - Mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-SP1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Chromatin Immunoprecipitation: SP1 Antibody [NB600-233] - Chromatin from acute myeloid leukemia cell line was immunoprecipitated with anti-Sp1 antibody and analyzed by DNA sequencing. The figure illustrates the peak ...read more
Immunohistochemistry-Paraffin: SP1 Antibody [NB600-233] - Human breast carcinoma. Antibody: Affinity purified rabbit anti-SP1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunoprecipitation: SP1 Antibody [NB600-233] - Detection of Human SP1 on HeLa whole cell lysate using NB600-233. SP1 was immunoprecipitated using affinity purified rabbit anti-SP1 antibody NB600-233. NB600-233 was used ...read more
Independent Antibodies: Immunoprecipitation: SP1 Antibody [NB600-233] - Detection of human SP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, IHC, IHC-P, IP, CHIP-SEQ
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

SP1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 750 and the C-terminus (residue 785) using the numbering given in entry XP_028606.4 (GeneID 6667).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2500-1:10000
  • Chromatin Immunoprecipitation 4 ug/30 ug lysate
  • Immunohistochemistry 1:2000-1:10000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1-4 ug/mg lysate
  • Chromatin Immunoprecipitation Sequencing
Control
SP1 Knockout HeLa Cell Lysate
Publications
Read Publications using
NB600-233 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Notes

This anitbody can be used as the primary antibody in a PLA assay with the following as complementing antibodies: NB100-55324, NB100-224, NB200-171, NB500-160, NB200-347

Alternate Names for SP1 Antibody

  • Sp1 transcription factor
  • specificity protein 1
  • transcription factor Sp1
  • TSFP1

Background

Sp1 is one of the first cellular transcription factors to be identified, cloned and characterized for its ability to bind to GC-boxes in regulatory promoter elements. Sp1 has always been described as a ubiquitous transcription factor that is required for the constitutive and inducible expression of a variety of genes, such as in cell cycle or mammalian development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SP1 Antibody (NB600-233)(4)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol

FAQs for SP1 Antibody (NB600-233) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB600-233AF350
Alexa Fluor 405 NB600-233AF405
Alexa Fluor 488 NB600-233AF488
Alexa Fluor 532 NB600-233AF532
Alexa Fluor 594 NB600-233AF594
Alexa Fluor 647 NB600-233AF647
Alexa Fluor 700 NB600-233AF700
Alexa Fluor 750 NB600-233AF750
Biotin NB600-233B
DyLight 350 NB600-233UV
DyLight 405 NB600-233V
DyLight 488 NB600-233G
DyLight 550 NB600-233R
DyLight 594 NB600-233DL594
DyLight 650 NB600-233C
DyLight 680 NB600-233FR
DyLight 755 NB600-233IR
FITC NB600-233F
HRP NB600-233H
Janelia Fluor 549 NB600-233JF549
Janelia Fluor 646 NB600-233JF646

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Bioinformatics Tool for SP1 Antibody (NB600-233)

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Research Areas for SP1 Antibody (NB600-233)

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Blogs on SP1.

Nur77 Activation and Tumor Suppression
Nur77 is a member of the steroid/thyroid hormone phosphoprotein receptor superfamily. It is heavily post-translationally modified and rapidly induced in response to androgens and growth factors. It governs fundamental processes such as cell proliferat...  Read full blog post.

Estrogen Related Receptors Play Roles in Cancer and Neurodegeneration
By Eric NeeleyEstrogen receptors come in the form of two distinct forms, ER alpha and ER beta. These nuclear receptors are predominantly activated by the hormone 17-beta-estradiol to control transcription of genes throughout the immune, nervous, car...  Read full blog post.

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Bioinformatics

Gene Symbol SP1
Entrez
Uniprot