SP1 Antibody


Biological Strategies: Western Blot: SP1 Antibody [NB600-233] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse Renca cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit ...read more
Immunohistochemistry-Paraffin: SP1 Antibody [NB600-233] - Mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-SP1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Chromatin Immunoprecipitation: SP1 Antibody [NB600-233] - Chromatin from acute myeloid leukemia cell line was immunoprecipitated with anti-Sp1 antibody and analyzed by DNA sequencing. The figure illustrates the peak ...read more
Immunohistochemistry-Paraffin: SP1 Antibody [NB600-233] - Human breast carcinoma. Antibody: Affinity purified rabbit anti-SP1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunoprecipitation: SP1 Antibody [NB600-233] - Detection of Human SP1 on HeLa whole cell lysate using NB600-233. SP1 was immunoprecipitated using affinity purified rabbit anti-SP1 antibody NB600-233. NB600-233 was used ...read more
Independent Antibodies: Immunoprecipitation: SP1 Antibody [NB600-233] - Detection of human SP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) ...read more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, IHC, IHC-P, IP, CHIP-SEQ
1.0 mg/ml

SP1 Antibody Summary

The immunogen recognized by this antibody maps to a region between residue 750 and the C-terminus (residue 785) using the numbering given in entry XP_028606.4 (GeneID 6667).
Immunogen affinity purified
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  • Western Blot 1:2000 - 1:10000
  • Chromatin Immunoprecipitation 4 ug/30 ug lysate
  • Immunohistochemistry 1:2000 - 1:10000
  • Immunohistochemistry-Paraffin 1:2000 - 1:10000
  • Immunoprecipitation 2-10 ug/mg lysate
  • Chromatin Immunoprecipitation Sequencing
Read Publications using
NB600-233 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
0.09% Sodium Azide
1.0 mg/ml
Immunogen affinity purified


This anitbody can be used as the primary antibody in a PLA assay with the following as complementing antibodies: NB100-55324, NB100-224, NB200-171, NB500-160, NB200-347

Alternate Names for SP1 Antibody

  • Sp1 transcription factor
  • specificity protein 1
  • transcription factor Sp1
  • TSFP1


Sp1 is one of the first cellular transcription factors to be identified, cloned and characterized for its ability to bind to GC-boxes in regulatory promoter elements. Sp1 has always been described as a ubiquitous transcription factor that is required for the constitutive and inducible expression of a variety of genes, such as in cell cycle or mammalian development.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SP1 Antibody (NB600-233)(4)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

FAQs for SP1 Antibody (NB600-233) (0)

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NB600-233AF350
Alexa Fluor 405 NB600-233AF405
Alexa Fluor 488 NB600-233AF488
Alexa Fluor 532 NB600-233AF532
Alexa Fluor 594 NB600-233AF594
Alexa Fluor 647 NB600-233AF647
Alexa Fluor 700 NB600-233AF700
Alexa Fluor 750 NB600-233AF750
Biotin NB600-233B
DyLight 350 NB600-233UV
DyLight 405 NB600-233V
DyLight 488 NB600-233G
DyLight 550 NB600-233R
DyLight 594 NB600-233DL594
DyLight 650 NB600-233C
DyLight 680 NB600-233FR
DyLight 755 NB600-233IR
FITC NB600-233F
HRP NB600-233H
Janelia Fluor 549 NB600-233JF549
Janelia Fluor 646 NB600-233JF646

Additional SP1 Products

Array NB600-233

Bioinformatics Tool for SP1 Antibody (NB600-233)

Discover related pathways, diseases and genes to SP1 Antibody (NB600-233). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for SP1 Antibody (NB600-233)

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PTMs for SP1 Antibody (NB600-233)

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Research Areas for SP1 Antibody (NB600-233)

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Blogs on SP1.

Nur77 Activation and Tumor Suppression
Nur77 is a member of the steroid/thyroid hormone phosphoprotein receptor superfamily. It is heavily post-translationally modified and rapidly induced in response to androgens and growth factors. It governs fundamental processes such as cell proliferat...  Read full blog post.

Estrogen Related Receptors Play Roles in Cancer and Neurodegeneration
By Eric NeeleyEstrogen receptors come in the form of two distinct forms, ER alpha and ER beta. These nuclear receptors are predominantly activated by the hormone 17-beta-estradiol to control transcription of genes throughout the immune, nervous, car...  Read full blog post.

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Gene Symbol SP1