RUNX2/CBFA1 Antibody - BSA Free

Images

 
Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody - BSA Free [NBP1-77461] - RUNX2/CBFA1 Antibody [NBP1-77461] - Vascularization of mineralized cell-laden collagen and interaction with prostate cancer cells. HUVECs ...read more
Immunocytochemistry/ Immunofluorescence: RUNX2/CBFA1 Antibody [NBP1-77461] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody - BSA Free [NBP1-77461] - RUNX2/CBFA1 Antibody [NBP1-77461] - Characterization of TLC cultured in chondrogenic pellets. Gene expression of TLC cultured four weeks in ...read more
Immunohistochemistry: RUNX2/CBFA1 Antibody [NBP1-77461] - Staining of RUNX2 in mouse prostate using DAB with hematoxylin counterstain.
Vascularization of mineralized cell-laden collagen and interaction with prostate cancer cells. b) HUVECs formed endothelial networks that were supported by alphaSMA-expressing hMSCs (scale bar: 50 um) and were also ...read more
H2A.X re-expression inhibits EMT and promotes metastatic colonization in the lung.(a) Immunoblot analysis of EMT markers in HCT116 parental cells (WT), H2A.X knockout cells (KO) and H2A.X knockout cells in which H2A.X ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

RUNX2/CBFA1 Antibody - BSA Free Summary

Immunogen
This RUNX2/CBFA1 Antibody was prepared from a synthetic peptide made to an internal region of the human RUNX2 protein (within residues 300-375). [Swiss-Prot Q13950]
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RUNX2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot reported in scientific literature (PMID 29208768)
Application Notes
Prior to immunostaining paraffin tissues antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Theoretical MW
56.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-77461 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RUNX2/CBFA1 Antibody - BSA Free

  • Acute myeloid leukemia 3 protein
  • CBFA1
  • CBF-alpha-1
  • CCD1
  • CCDAML3
  • CLCD
  • Core-binding factor subunit alpha-1
  • core-binding factor, runt domain, alpha subunit 1
  • MGC120023
  • ML3
  • oncogene AML-3
  • OSF2
  • OSF-2
  • osteoblast-specific transcription factor 2
  • PEA2aA
  • PEA2-alpha A
  • PEBP2A
  • PEBP2aA
  • PEBP2-alpha A
  • polyomavirus enhancer-binding protein 2 alpha A subunit
  • runt domain, alpha subunit 1
  • runt related transcription factor 2
  • runt-related transcription factor 2
  • RUNX2
  • SL3/AKV core-binding factor alpha A subunit
  • SL3-3 enhancer factor 1 alpha A subunit

Background

Runt-related transcription factor 2 (RUNX2), also known as CBFA1, AML-3, PEBP-2alphaA, and OSF-2, is a transcription factor that places a critical role in osteoblast differentiation and bone development (1-3). RUNX2 is a DNA-binding protein that belongs to the RUNX family which share a common runt domain (3). RUNX2 has two main isoforms which vary based on the two promoter regions (3). The main canonical isoform (P1) has MASN/DS at its N-terminus while the other (P2) isoform includes a MRIPV pentapeptide at its N-terminus (3). The RUNX2 P1 isoform has a theoretical molecular weight of 56 kDa and is synthesized as a 521 amino acid (aa) protein containing multiple domains. Specifically, RUNX2 contains transactivation domains (AD1, 2 and 3), a glutamine/alanine (Q/A)-rich domain, a runt homology domain (RHD), a nuclear localization signal (NLS), a proline/serine/threonine (PST)-rich domain, a nuclear matrix targeting signal (NMTS), a repression domain (RD), and a VWRPY region (3). RUNX2 is a heterodimer of an alpha and beta subunit where the alpha subunit binds DNA through the runt domain and the binding affinity is increased through heterodimerization (4).

Functionally, RUNX2 promotes the expression of osteoblast-specific genes vital for the osteoblast differentiation and proliferation process including type I collagen, osteocalcin (OCN), and alkaline phosphatase (APC) (1, 3). Further evidence for the role of RUNX2 is highlighted by a study of Runx2-/-mice which completely lack osteoblasts (4). Additionally, RUNX2 is also required for chondrocyte maturation, which are the cells responsible for cartilage formation (1, 3, 5). Given the role of RUNX2 in bone and cartilage maturation and formation, it is clear that defects or mutations in RUNX2 cause various bone and bone-related diseases (3, 6, 7). For instance, cleidocranial dysplasia (CCD), which presents with delayed cranial suture closure phenotypes, hypoplastic clavicles, extra teeth, and short stature, is caused by haploinsufficiency in RUNX2 (2, 3, 6). Furthermore, metaphyseal dysplasia with maxillary hypoplasia and brachydactyly (MDMHB) is a bone dysplasia disorder with a phenotype of abnormalities in the long bones, an underdeveloped jawbone, and short fingers that is caused by a duplication in RUNX2 (6). Finally, RUNX2 has been shown to be upregulated in mouse models of the joint disorder osteoarthritis (OA) and may be a potential molecular target for disease treatment (7).

Alternative names for RUNX2 include Acute myeloid leukemia 3 protein CBFA1, CBF-alpha-1, CCD1, CCDAML3, CLCD, Core-binding factor subunit alpha-1, MGC120023, ML3, oncogene AML-3, OSF2, osteoblast-specific transcription factor 2, PEA2aA, PEA2-alpha A, PEBP2A, polyomavirus enhancer-binding protein 2 alpha A subunit, runt related transcription factor 2, SL3/AKV core-binding factor alpha A subunit, and SL3-3 enhancer factor 1 alpha A subunit.

References

1. Ferreira, L. B., Gimba, E., Vinagre, J., Sobrinho-Simoes, M., & Soares, P. (2020). Molecular Aspects of Thyroid Calcification. International journal of molecular sciences. https://doi.org/10.3390/ijms21207718

2. Kim, W. J., Shin, H. L., Kim, B. S., Kim, H. J., & Ryoo, H. M. (2020). RUNX2-modifying enzymes: therapeutic targets for bone diseases. Experimental & molecular medicine. https://doi.org/10.1038/s12276-020-0471-4

3. Vimalraj, S., Arumugam, B., Miranda, P. J., & Selvamurugan, N. (2015). Runx2: Structure, function, and phosphorylation in osteoblast differentiation. International journal of biological macromolecules. https://doi.org/10.1016/j.ijbiomac.2015.04.008

4. Uniprot (Q13950)

5. Komori T. (2017). Roles of Runx2 in Skeletal Development. Advances in experimental medicine and biology. https://doi.org/10.1007/978-981-10-3233-2_6

6. Moffatt, P., Ben Amor, M., Glorieux, F. H., Roschger, P., Klaushofer, K., Schwartzentruber, J. A., Paterson, A. D., Hu, P., Marshall, C., FORGE Canada Consortium, Fahiminiya, S., Majewski, J., Beaulieu, C. L., Boycott, K. M., & Rauch, F. (2013). Metaphyseal dysplasia with maxillary hypoplasia and brachydactyly is caused by a duplication in RUNX2. American journal of human genetics. https://doi.org/10.1016/j.ajhg.2012.12.001

7. Chen, D., Kim, D. J., Shen, J., Zou, Z., & O'Keefe, R. J. (2019). Runx2 plays a central role in Osteoarthritis development. Journal of orthopaedic translation. https://doi.org/10.1016/j.jot.2019.11.008

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RUNX2/CBFA1 Antibody (NBP1-77461)(17)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ICC/IF, IF/IHC, IHC-P, In Vivo, WB.


Filter By Application
ICC/IF
(5)
IF/IHC
(2)
IHC-P
(1)
In Vivo
(1)
WB
(2)
All Applications
Filter By Species
Human
(5)
Mouse
(5)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using NBP1-77461 Applications Species
Sousa MGDC, Balbinot GS, Subbiah R et al. In vitro development and optimization of cell-laden injectable bioprinted gelatin methacryloyl (GelMA) microgels mineralized on the nanoscale bioRxiv : the preprint server for biology 2023-10-12 [PMID: 37873385] (ICC/IF, Mouse) ICC/IF Mouse
Chan, B;Glogauer, M;Wang, Y;Wrana, J;Chan, K;Beier, F;Bali, S;Hinz, B;Parreno, J;Ashraf, S;Kandel, R; Adseverin, an actin-binding protein, modulates hypertrophic chondrocyte differentiation and osteoarthritis progression Science advances 2023-08-04 [PMID: 37540756] (In Vivo) In Vivo
Poudel SB, Ruff RR, Yildirim G et al. Excess growth hormone triggers inflammation-associated arthropathy, subchondral bone loss, and arthralgia The American journal of pathology 2023-03-02 [PMID: 36870529] (IHC-P, Mouse) IHC-P Mouse
Subbiah R, Lin EY, Athirasala A et al. Engineering of an osteoinductive and growth factor-free injectable bone-like microgel for bone regeneration Advanced healthcare materials 2023-02-20 [PMID: 36808718]
Zanut A, Li R, Deng R et al. A polymer canvas with the stiffness of the bone matrix to study and control mesenchymal stem cell response Advanced healthcare materials 2022-12-24 [PMID: 36565136]
Ciavarella C, Motta I, Vasuri F et al. Involvement of miR-30a-5p and miR-30d in Endothelial to Mesenchymal Transition and Early Osteogenic Commitment under Inflammatory Stress in HUVEC Biomolecules 2021-02-05 [PMID: 33562690] (ICC/IF, Human) ICC/IF Human
Serjeant M, Moon PM, Quinonez D, et al. The Role of Panx3 in Age-Associated and Injury-Induced Intervertebral Disc Degeneration International journal of molecular sciences 2021-01-22 [PMID: 33499145] (IF/IHC, Mouse) IF/IHC Mouse
Cahill SV, Kwon HK, Back J et al. Locally-delivered Adjuvant Biofilm-penetrating Antibiotics Rescue Impaired Endochondral Fracture Healing Caused by MRSA Infection Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2020-12-18 [PMID: 33336805]
Chen PY, Qin L, Li G et al. Smooth Muscle Cell Reprogramming in Aortic Aneurysms Cell Stem Cell 2020-04-02 [PMID: 32243809] (Mouse) Mouse
Darrieutort-Laffite C, Arnolfo P, Garraud T et al. Rotator Cuff Tenocytes Differentiate into Hypertrophic Chondrocyte-Like Cells to Produce Calcium Deposits in an Alkaline Phosphatase-Dependent Manner J Clin Med [PMID: 31561454] (IF/IHC) IF/IHC
Show All 17 Publications.

Reviews for RUNX2/CBFA1 Antibody (NBP1-77461) (0)

There are no reviews for RUNX2/CBFA1 Antibody (NBP1-77461). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RUNX2/CBFA1 Antibody (NBP1-77461). (Showing 1 - 2 of 2 FAQs).

  1. We would like an anti-RUNX2 for IHC-P which share cross reactivity with Rat, but not with Human.
    • We don't have any data for our RUNX2 antibodies that confirms they will NOT detect the human protein. When we can confirm that an antibody will not react with a certain species, we display a (-) sign on the datasheet. Otherwise, if the species is not listed it means that it has not been tested.
  2. I want to chase Runx2/CBFA1 antibody (NBP1-77461). Does it work with frozen section?
    • Our lab has never tested this antibody in IHC-Fr (frozen sections). NBP1-77461 has only be validated in ICC/IF and IHC-P. Therefore we don't know whether this antibody can produce the positive results in your experiments. However our experiences tell us that, as a general rule, the antibodies that can be used in IHC-P, they are likely to produce the positive results in IHC-Fr after some optimizing experiments. If you indeed use NBP1-77461 in IHC-Fr, you will be automatically qualified for our Innovator's Reward that earn a free antibody from us.

Secondary Antibodies

 

Isotype Controls

Additional RUNX2/CBFA1 Products

Research Areas for RUNX2/CBFA1 Antibody (NBP1-77461)

Find related products by research area.

Blogs on RUNX2/CBFA1

There are no specific blogs for RUNX2/CBFA1, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our RUNX2/CBFA1 Antibody - BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol RUNX2
Entrez
Uniprot