Immunocytochemistry/ Immunofluorescence: RUNX2/CBFA1 Antibody [NBP1-77461] - RUNX2 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunohistochemistry: RUNX2/CBFA1 Antibody [NBP1-77461] - Staining of RUNX2 in mouse prostate using DAB with hematoxylin counterstain.
This RUNX2 antibody is useful for ICC/IF and Immunohistochemistry-paraffin embedded sections. Use in Western Blot reported in scientific literature (PMID: 29208768). Prior to immunostaining paraffin tissues antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.05% Sodium Azide
Immunogen affinity purified
Alternate Names for RUNX2/CBFA1 Antibody
Acute myeloid leukemia 3 protein
Core-binding factor subunit alpha-1
runt domain, alpha subunit 1
runt-related transcription factor 2
SL3/AKV core-binding factor alpha A subunit
SL3-3 enhancer factor 1 alpha A subunit
RUNX2 is a transcription factor that mediates the expression several downstream proteins, which affect osteoblast differentiation and mitotic progression. It also is important in monocyte activation, as it is upregulated in response to LPS infection. Through interaction with Twist 1 and Twist 2, RUNX2 regulates osteocalcin transcription, following parathyroid hormone signaling for bone loss repair. Ossification in skeletal formation is also partially regulated through RUNX2. Essentially, this transcription factor is responsible for many developmental and repair mechanisms in bone and collagen.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for RUNX2/CBFA1 Antibody (NBP1-77461). (Showing 1 - 2 of 2 FAQs).
We would like an anti-RUNX2 for IHC-P which share cross reactivity with Rat, but not with Human.
We don't have any data for our RUNX2 antibodies that confirms they will NOT detect the human protein. When we can confirm that an antibody will not react with a certain species, we display a (-) sign on the datasheet. Otherwise, if the species is not listed it means that it has not been tested.
I want to chase Runx2/CBFA1 antibody (NBP1-77461). Does it work with frozen section?
Our lab has never tested this antibody in IHC-Fr (frozen sections). NBP1-77461 has only be validated in ICC/IF and IHC-P. Therefore we don't know whether this antibody can produce the positive results in your experiments. However our experiences tell us that, as a general rule, the antibodies that can be used in IHC-P, they are likely to produce the positive results in IHC-Fr after some optimizing experiments. If you indeed use NBP1-77461 in IHC-Fr, you will be automatically qualified for our Innovator's Reward that earn a free antibody from us.
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