RUNX2/CBFA1 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: RUNX2/CBFA1 Antibody [NBP1-77461] - RUNX2 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunohistochemistry: RUNX2/CBFA1 Antibody [NBP1-77461] - Staining of RUNX2 in mouse prostate using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

RUNX2/CBFA1 Antibody Summary

Immunogen
A synthetic peptide made to an internal region of the human RUNX2 protein (within residues 300-375). [Swiss-Prot Q13950]
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RUNX2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
Application Notes
This RUNX2 antibody is useful for ICC/IF and Immunohistochemistry-paraffin embedded sections. Use in Western Blot reported in scientific literature (PMID: 29208768). Prior to immunostaining paraffin tissues antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Control
RUNX2/CBFA1 Knockout HeLa Cell Lysate
Publications
Read Publications using
NBP1-77461 in the following applications:

  • 4 publications
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RUNX2/CBFA1 Antibody

  • Acute myeloid leukemia 3 protein
  • AML3CCD
  • CBFA1
  • CBFA1MGC120022
  • Core-binding factor subunit alpha-1
  • MGC120023
  • PEA2-alpha A
  • PEBP2A
  • PEBP2A1CCD1
  • PEBP2aA1
  • PEBP2-alpha A
  • runt domain, alpha subunit 1
  • runt-related transcription factor 2
  • RUNX2
  • SL3/AKV core-binding factor alpha A subunit
  • SL3-3 enhancer factor 1 alpha A subunit

Background

RUNX2 is a transcription factor that mediates the expression several downstream proteins, which affect osteoblast differentiation and mitotic progression. It also is important in monocyte activation, as it is upregulated in response to LPS infection. Through interaction with Twist 1 and Twist 2, RUNX2 regulates osteocalcin transcription, following parathyroid hormone signaling for bone loss repair. Ossification in skeletal formation is also partially regulated through RUNX2. Essentially, this transcription factor is responsible for many developmental and repair mechanisms in bone and collagen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RUNX2/CBFA1 Antibody (NBP1-77461)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(4)
WB
(2)
All Applications
Filter By Species
Human
(5)
Mouse
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using NBP1-77461 Applications Species
Priante G, Quaggio F, Gianesello L et al. Caspase-independent programmed cell death triggers Ca2PO4 deposition in an in vitro model of nephrocalcinosis. Biosci. Rep. 2017 Dec 05 [PMID: 29208768] (WB, Human) WB Human
Pillai IC, Li S, Romay M et al. Cardiac Fibroblasts Adopt Osteogenic Fates and Can Be Targeted to Attenuate Pathological Heart Calcification. Cell Stem Cell. Nov 15 2016 [PMID: 27867037]
Mao AS, Shin JW, Utech S et al. Deterministic encapsulation of single cells in thin tunable microgels for niche modelling and therapeutic delivery. Nat Mater. 2016 Oct 31 [PMID: 27798621]

Details:
This citation used the Alexa Fluor 647 version of this antibody.
Al-Rekabi Z, Wheeler MM, Leonard A et al. Activation of the IGF1 Pathway Mediates Changes in Cellular Contractility and Motility in Single-Suture Craniosynostosis J Cell Sci (ICC/IF, Human)

Details:
This publication used the DyLight 488 conjugated form of this antibody (Cat# NBP1-77461G).
ICC/IF Human
Thrivikraman G, Athirasala A, Gordon R et al. Rapid fabrication of vascularized and innervated cell-laden bone models with biomimetic intrafibrillar collagen mineralization Nat Commun Aug 6 2019 [PMID: 31388010] (ICC/IF, Human) ICC/IF Human
Priante G, Ceol M, Gianesello L et al. Human proximal tubular cells can form calcium phosphate deposits in osteogenic culture: role of cell death and osteoblast-like transdifferentiation. Cell Death Discov Jan 28 2019 [PMID: 30701089] (WB, Human) WB Human
Mao AS, Shin JW, Mooney DJ. Effects of substrate stiffness and cell-cell contact on mesenchymal stem cell differentiation. Biomaterials. 2016 May 5 [PMID: 27203745] (ICC/IF, Mouse)

Details:
This publication used the AF647 conjugated form of this antibody (NBP1-77461AF647)
ICC/IF Mouse
Al-Rekabi Z, Wheeler MM, Leonard A et al. Activation of the IGF1 Pathway Mediates Changes in Cellular Contractility and Motility in Single-Suture Craniosynostosis J Cell Sci 2015 Dec 11 [PMID: 26659664] (ICC/IF, Human)

Details:
This citation used the DyLight 488 version of this antibody.
ICC/IF Human

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RUNX2/CBFA1 Antibody (NBP1-77461). (Showing 1 - 2 of 2 FAQs).

  1. We would like an anti-RUNX2 for IHC-P which share cross reactivity with Rat, but not with Human.
    • We don't have any data for our RUNX2 antibodies that confirms they will NOT detect the human protein. When we can confirm that an antibody will not react with a certain species, we display a (-) sign on the datasheet. Otherwise, if the species is not listed it means that it has not been tested.
  2. I want to chase Runx2/CBFA1 antibody (NBP1-77461). Does it work with frozen section?
    • Our lab has never tested this antibody in IHC-Fr (frozen sections). NBP1-77461 has only be validated in ICC/IF and IHC-P. Therefore we don't know whether this antibody can produce the positive results in your experiments. However our experiences tell us that, as a general rule, the antibodies that can be used in IHC-P, they are likely to produce the positive results in IHC-Fr after some optimizing experiments. If you indeed use NBP1-77461 in IHC-Fr, you will be automatically qualified for our Innovator's Reward that earn a free antibody from us.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-77461AF350
Alexa Fluor 405 NBP1-77461AF405
Alexa Fluor 488 NBP1-77461AF488
Alexa Fluor 532 NBP1-77461AF532
Alexa Fluor 594 NBP1-77461AF594
Alexa Fluor 647 NBP1-77461AF647
Alexa Fluor 700 NBP1-77461AF700
Alexa Fluor 750 NBP1-77461AF750
Biotin NBP1-77461B
DyLight 350 NBP1-77461UV
DyLight 405 NBP1-77461V
DyLight 488 NBP1-77461G
DyLight 550 NBP1-77461R
DyLight 594 NBP1-77461DL594
DyLight 650 NBP1-77461C
DyLight 680 NBP1-77461FR
DyLight 755 NBP1-77461IR
FITC NBP1-77461F
Janelia Fluor 549 NBP1-77461JF549
Janelia Fluor 646 NBP1-77461JF646

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Bioinformatics Tool for RUNX2/CBFA1 Antibody (NBP1-77461)

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Bioinformatics

Gene Symbol RUNX2
Entrez
Uniprot