Collagen I alpha 1 Antibody (COL-1)

Images

 
Immunohistochemistry-Frozen: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Staining of acetone-fixed, frozen sections of pig tongue at a dilution of 1:4000
Immunocytochemistry/ Immunofluorescence: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Collagen I alpha1 labeled pig trabecular meshwork cells indirectly visualized with Alexa Fluor 488 conjugated secondary ...read more
Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Analysis of an FFPE ligament section. Enzymatic antigen retrieval and blocking using 1% BSA for 30 min at 20C was performed. Sample was incubated ...read more
Immunohistochemistry-Frozen: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Staining of acetone-fixed, frozen sections of human tongue at a dilution of 1:2000.
Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Analysis of formalin fixed frozen porcine skin tissue sections. Blocking: 5% serum for 1 hr at 20C. Sample was incubated for 16 hours at 4C and ...read more
Immunocytochemistry/ Immunofluorescence: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Analysis of Collagen I alpha 1 in 4% PFA fixed equine tenocytes using anti-Collagen I alpha 1 antibody. Image from verified ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Eq, Fe, Ma, RbSpecies Glossary
Applications WB, DB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
COL-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Collagen I alpha 1 Antibody (COL-1) Summary

Immunogen
This Collagen I alpha 1 antibody (COL-1) was raised against full length bovine native protein (purified).
Epitope
The epitope recognized by the antibody may be sensitive to routine formalin fixation and paraffin embedding.
Localization
Extracellular matrix
Specificity
This Collagen I alpha 1 antibody (COL-1) recognizes the native (helical) form of collagen type I using ELISA and dot-blot. It does not react with the thermally-denatured molecule, and shows no cross-reactivity with collagen types II, III, IV, V, VI, VII, IX, X and XI.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
COL1A1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Dot Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:2000
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Use in WB reported in scientifc literature (PMID: 30486863). If using for WB, we recommend performing immunoblotting in non-reducing conditions. In IHC testing of acetone-fixed or unfixed frozen sections, strong staining of connective tissue fibers is seen. This antibody has been used in IHC as reported in the literature (PMID: 22019120) including IHC-Paraffin (PMID 24242003) and ICC (PMID 27769912). Use in Flow Cytometry reported by customer review.
Theoretical MW
139 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 4 Reviews rated 4.8
using
NB600-450 in the following applications:

Publications
Read Publications using
NB600-450 in the following applications:

Reactivity Notes

Reported reactivity in deer. Mouse reactivity reported in scientific literature (PMID: 25287675). Goat reactivity reported by customer review. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
15mM Sodium Azide
Purity
Unpurified

Alternate Names for Collagen I alpha 1 Antibody (COL-1)

  • Alpha-1 type I collagen
  • COL1A1
  • Collagen 1 alpha 1
  • Collagen 1
  • collagen alpha 1 chain type I
  • collagen alpha-1(I) chain
  • Collagen I alpha 1
  • collagen of skin, tendon and bone, alpha-1 chain
  • Collagen Type I Alpha 1 Chain
  • collagen, type I, alpha 1
  • Collagen1
  • Collagen-1
  • EDSARTH1
  • OI4
  • pro-alpha-1 collagen type 1
  • Type I Procollagen Alpha 1 Chain

Background

Collagen is an extracellular matrix protein that serves as a scaffold defining the shape and mechanical properties of many tissues and organs including skin, tendon, artery walls, fibrocartilage, bone and teeth. Collagens are highly conserved and are characterized by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. The extensive family of collagens is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagen type I (also known as collagen alpha, COL1A1, and alpha-1 type I collagen) is the largest component of fibrillar collagen found in cartilage and connective tissues. It is synthesized by fibroblasts, osteoblasts, and odontoblasts and has a theoretical molecular weight of 138 kDa.

Type I collagen is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon tissue. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue. Several collagens play a role in cell adhesion, responsible for maintaining normal tissue architecture and function. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Post-Golgi LH3 trafficking is essential for collagen homeostasis and for the development and function of multiple organs and tissues (1).

The COL1A1 gene encodes the pro-alpha1 chains of type I collagen protein, whose triple helix is comprised of two alpha1 chains and one alpha2 chain. Mutations in the encoding COL1A1 gene are associated with brittle bone disease (Osteogenesis Imperfecta), cortical hyperostosis (Caffey disease) and disorders that affect the connective tissues (Ehlers-Danlos syndrome) (2). Studies have found that HIF-1 transcription regulation of collagen prolyl hydroxylases regulates collagen deposition, promoting cancer cell alignment along collagen fibers, which enhances invasion and metastasis to lymph nodes and lung tissue by breast cancer cells (3).

References

1. Banushi, B., Forneris, F., Straatman-Iwanowska, A., Strange, A., Lyne, A. M., Rogerson, C.,... Gissen, P. (2016). Regulation of post-Golgi LH3 trafficking is essential for collagen homeostasis. Nat Commun, 7, 12111. doi:10.1038/ncomms12111

2. Lu, Y., Zhang, S., Wang, Y., Ren, X., & Han, J. (2019). Molecular mechanisms and clinical manifestations of rare genetic disorders associated with type I collagen. Intractable Rare Dis Res, 8(2), 98-107. doi:10.5582/irdr.2019.01064

3. Gilkes, D. M., Chaturvedi, P., Bajpai, S., Wong, C. C., Wei, H., Pitcairn, S.,... Semenza, G. L. (2013). Collagen prolyl hydroxylases are essential for breast cancer metastasis. Cancer Res, 73(11), 3285-3296. doi:10.1158/0008-5472.Can-12-3963

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Collagen I alpha 1 Antibody (NB600-450)(35)

We have publications tested in 4 confirmed species: Human, Mouse, Equine, Rabbit.

We have publications tested in 6 applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ELISA
(1)
ICC/IF
(3)
IHC
(13)
IHC-Fr
(1)
IHC-P
(8)
WB
(1)
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Filter By Species
Human
(12)
Mouse
(6)
Equine
(1)
Rabbit
(6)
All Species
Showing Publications 1 - 10 of 35. Show All 35 Publications.
Publications using NB600-450 Applications Species
Liu J, Yu C, Chen Y et al. Fast fabrication of stable cartilage-like tissue using collagen hydrogel microsphere culture Journal of Materials Chemistry B 2017 Nov 01 (IHC) IHC
Costa D, Vene R, Benelli R et al. Targeting the Epidermal Growth Factor Receptor Can Counteract the Inhibition of Natural Killer Cell Function Exerted by Colorectal Tumor-Associated Fibroblasts Front Immunol May 29 2018 [PMID: 29910806] (Human) Human
Kuzan A, Chwilkowska A, Maksymowicz K et al. Advanced glycation end products as a source of artifacts in immunoenzymatic methods Glycoconj. J. 2018 Jan 05 [PMID: 29305778] (ELISA, Human) ELISA Human
Ramani K, Tomasi ML, Berlind J et al. Role of A-Kinase Anchoring Protein Phosphorylation in Alcohol-Induced Liver Injury and Hepatic Stellate Cell Activation Am. J. Pathol. 2018 Jan 02 [PMID: 29305319] (Human) Human
Wegner K, Keikhosravi A, Eliceiri K et al. Fluorescence of Picrosirius Red Multiplexed With Immunohistochemistry for the Quantitative Assessment of Collagen in Tissue Sections. J Histochem Cytochem 2017 Jul 10 [PMID: 28692327] (IHC-P, Mouse) IHC-P Mouse
da Silva IR, Tiveron LCRDC, da Silva MV et al. In Situ Cytokine Expression and Morphometric Evaluation of Total Collagen and Collagens Type I and Type III in Keloid Scars. Mediators Inflamm. 2017 Jun 22 [PMID: 28638180] (IHC, Human) IHC Human
Bono N, Meghezi S, Soncini M et al. A Dual-Mode Bioreactor System for Tissue Engineered Vascular Models. Ann Biomed Eng. 2017 Feb 21 [PMID: 28224370]
Sa G, Xiong X, Ren J et al. Expression of fibrosis-related molecules in the oral mucosa of six animal species: A reference for selecting animal models. Eur J Pharm Sci Oct 18 2016 [PMID: 27769912] (IHC, ICC/IF, Human) IHC, ICC/IF Human
Karti O, Zengin MO, Cinar E et al. Effect of 1- and 6-Hour-Delayed Corneal Collagen Cross-Linking on Corneal Healing in a Rabbit Alkali-Burn Model: Clinical and Histological Observations. Cornea. Oct 12 2016 [PMID: 27741014]
Loy C, Laine A, Mantovani D. Rotation-based technique for the rapid densification of tubular collagen gel scaffolds. Biotechnol J 2016 Dec [PMID: 27731544]
Show All 35 Publications.

Reviews for Collagen I alpha 1 Antibody (NB600-450) (4) 4.84

Average Rating: 4.8
(Based on 4 reviews)
We have 4 reviews tested in 4 species: Feline, Goat, Other, Pig.

Reviews using NB600-450:
Filter by Applications
Flow
(1)
IHC-Fr
(1)
ICC
(1)
IF
(1)
All Applications
Filter by Species
Feline
(1)
Goat
(1)
Other
(1)
Pig
(1)
All Species
Images Ratings Applications Species Date Details
Flow Cytometry Collagen I alpha 1 NB600-450
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reviewed by:
Anonymous
Flow Goat 01/22/2019
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Summary

ApplicationFlow Cytometry
Sample TestedChondrocyte
SpeciesGoat
Lot067M4805V
Immunohistochemistry-Frozen Collagen I alpha 1 NB600-450
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reviewed by:
Anonymous
IHC-Fr Feline 09/15/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSclera
SpeciesFeline
Lot067M4805V
Immunocytochemistry Collagen I alpha 1 NB600-450
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reviewed by:
Anonymous
ICC Pig 06/07/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedPig trabecular meshwork cells
SpeciesPig
LotN/A
Immunofluorescence Collagen I alpha 1 NB600-450
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reviewed by:
Jessica Ochoa
IF Other 04/21/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedEquine Tenocytes
SpeciesOther

Blocking

Blocking Details5% BSA in 1X PBS, 20 minutes, room temperature

Primary Anitbody

Dilution Ratio1:2000, 2 hours, room temperature

Secondary Antibody

Secondary DescriptionGoat Anti-Mouse IgG, Alexa Fluor 594
Secondary Manufacturer Cat#A11005
Secondary Concentration1:500 dilution

Details

Detection NotesHT-1080 (positive control), Human PBMCs (negative control)
Fixation Details4% PFA fix for 20 minutes at room temperature; 30 minute permeabilization with 0.01% Tween 20
Wash Description1X PBS, 5 minutes, 3 washes

Product General Protocols

View specific protocols for Collagen I alpha 1 Antibody (NB600-450): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Collagen I alpha 1 Antibody (NB600-450). (Showing 1 - 5 of 5 FAQs).

  1. We are looking for an anti-Collagen antibody for Flow Cytometry (FACS) analysis in Swine sample. Would you please provide some references if there is any available as well?
    • We currently have one antibody for porcine Collagen validated in flow cytometry (catalog # NBP1-94202).
  2. I am looking for anti collagen 1 antibody, I see your product calls it collagen I alpha 1 is there a difference?
    • Collagen I alpha 1 is the most common protein for the collagen 1 protein. There are other subunits as well (such as collagen 1 alpha 2…etc.).
  3. The predicted molecular weight says 150kDA but the band on the gel appears at 130kDA why is that?
    • Differences between the predicted and observed MW can be affected by a number of factors including PTMs, relative charges and experimental factors. These cumulative effect of these differences can be quite substantial with larger molecules such as collagen I alpha 1.
  4. How long does delivery take for your collagen 1 alpha 1 antibody?
    • Our most popular collagen I alpha 1 antibody (NB600-408) is usually in stock and ships priority overnight for next business day delivery in the U.S.
  5. What is the dilution factor for IHC for starting? Because the range of dilution factor on the datasheet is so broad, I am confused which dilution factor is suitable for starting.
    • I can recommend the following dilution to start. Keep in mind that these will all vary greatly by sample type, so these are just suggestions. You may need to use more or less antibody. For catalog number NB600-450, the dilution range is 1:1000-1:2000.

Secondary Antibodies

 

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Recent Reviews

4.8
5
3
4
1
3
0
2
0
1
0

Anonymous
01/22/2019
Application: Flow
Species: Goat

Anonymous
09/15/2018
Application: IHC-Fr
Species: Feline

Anonymous
06/07/2017
Application: ICC
Species: Pig

Bioinformatics

Gene Symbol COL1A1
Entrez