Collagen I alpha 1 Antibody (COL-1)

Images

 
Immunohistochemistry-Frozen: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Staining of acetone-fixed, frozen sections of pig tongue at a dilution of 1:4000
Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Analysis of an FFPE ligament section. Enzymatic antigen retrieval and blocking using 1% BSA for 30 min at 20C was performed. Sample was incubated ...read more
Immunocytochemistry/ Immunofluorescence: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Collagen I and collagen III accumulate in C57BL/6 mice in response to radiation. Bladder strips from irradiated and control mice ...read more
Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Staining of acetone-fixed, frozen sections of human tongue at a dilution of 1:2000.
Immunocytochemistry/ Immunofluorescence: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Analysis of Collagen I alpha 1 in 4% PFA fixed equine tenocytes using anti-Collagen I alpha 1 antibody. ICC/IF image submitted ...read more
Immunocytochemistry/ Immunofluorescence: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Collagen I alpha1 labeled pig trabecular meshwork cells indirectly visualized with Alexa Fluor 488 conjugated secondary ...read more
Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Analysis of formalin fixed frozen porcine skin tissue sections. Blocking: 5% serum for 1 hr at 20C. Sample was incubated for 16 hours at 4C and ...read more
Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Immunohistochemistry for type I collagen in keloid (20x). Image collected and cropped by CiteAb from the following publication ...read more
Immunohistochemistry-Paraffin: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Mouse healthy liver. Anibody at 1:200. Enzymatic/proteolytic antigen retrieval. No permeabilization. IHC-P image submitted by a verified ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Eq, Fe, Ma, RbSpecies Glossary
Applications WB, DB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
COL-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Collagen I alpha 1 Antibody (COL-1) Summary

Immunogen
This Collagen I alpha 1 antibody (COL-1) was raised against full length bovine native protein (purified).
Epitope
The epitope recognized by the antibody may be sensitive to routine formalin fixation and paraffin embedding.
Localization
Extracellular matrix
Specificity
This Collagen I alpha 1 antibody (COL-1) recognizes the native (helical) form of collagen type I using ELISA and dot-blot. It does not react with the thermally-denatured molecule, and shows no cross-reactivity with collagen types II, III, IV, V, VI, VII, IX, X and XI.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
COL1A1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Dot Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:2000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
Theoretical MW
139 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 5 Reviews rated 4.6
using
NB600-450 in the following applications:

Publications
Read Publications using
NB600-450 in the following applications:

Reactivity Notes

Reported reactivity in deer. Mouse reactivity reported in scientific literature (PMID: 25287675). Goat reactivity reported by customer review. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions. Feline reactivity reported in scientific literature (PMID:33091431).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
15mM Sodium Azide
Purity
Unpurified

Alternate Names for Collagen I alpha 1 Antibody (COL-1)

  • Alpha-1 type I collagen
  • COL1A1
  • COL-IA1
  • Collagen 1 alpha 1
  • Collagen 1
  • collagen alpha 1 chain type I
  • collagen alpha-1(I) chain
  • Collagen I alpha 1
  • collagen of skin, tendon and bone, alpha-1 chain
  • Collagen Type I Alpha 1 Chain
  • collagen, type I, alpha 1
  • Collagen1
  • Collagen-1
  • EDSARTH1
  • OI4
  • pro-alpha-1 collagen type 1
  • Type I Procollagen Alpha 1 Chain

Background

Collagen is an extracellular matrix protein that serves as a scaffold defining the shape and mechanical properties of many tissues and organs including skin, tendon, artery walls, fibrocartilage, bone and teeth. Collagens are highly conserved and are characterized by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. The extensive family of collagens is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagen type I (also known as collagen alpha, COL1A1, and alpha-1 type I collagen) is the largest component of fibrillar collagen found in cartilage and connective tissues. It is synthesized by fibroblasts, osteoblasts, and odontoblasts and has a theoretical molecular weight of 138 kDa.

Type I collagen is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon tissue. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue. Several collagens play a role in cell adhesion, responsible for maintaining normal tissue architecture and function. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Post-Golgi LH3 trafficking is essential for collagen homeostasis and for the development and function of multiple organs and tissues (1).

The COL1A1 gene encodes the pro-alpha1 chains of type I collagen protein, whose triple helix is comprised of two alpha1 chains and one alpha2 chain. Mutations in the encoding COL1A1 gene are associated with brittle bone disease (Osteogenesis Imperfecta), cortical hyperostosis (Caffey disease) and disorders that affect the connective tissues (Ehlers-Danlos syndrome) (2). Studies have found that HIF-1 transcription regulation of collagen prolyl hydroxylases regulates collagen deposition, promoting cancer cell alignment along collagen fibers, which enhances invasion and metastasis to lymph nodes and lung tissue by breast cancer cells (3).

References

1. Banushi, B., Forneris, F., Straatman-Iwanowska, A., Strange, A., Lyne, A. M., Rogerson, C., . . . Gissen, P. (2016). Regulation of post-Golgi LH3 trafficking is essential for collagen homeostasis. Nat Commun, 7, 12111. doi:10.1038/ncomms12111

2. Lu, Y., Zhang, S., Wang, Y., Ren, X., & Han, J. (2019). Molecular mechanisms and clinical manifestations of rare genetic disorders associated with type I collagen. Intractable Rare Dis Res, 8(2), 98-107. doi:10.5582/irdr.2019.01064

3. Gilkes, D. M., Chaturvedi, P., Bajpai, S., Wong, C. C., Wei, H., Pitcairn, S., . . . Semenza, G. L. (2013). Collagen prolyl hydroxylases are essential for breast cancer metastasis. Cancer Res, 73(11), 3285-3296. doi:10.1158/0008-5472.Can-12-3963

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Collagen I alpha 1 Antibody (NB600-450)(48)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Equine, Feline, Rabbit.

We have publications tested in 6 applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ELISA
(1)
ICC/IF
(5)
IHC
(15)
IHC-Fr
(3)
IHC-P
(8)
WB
(1)
All Applications
Filter By Species
Human
(10)
Mouse
(6)
Rat
(5)
Equine
(1)
Feline
(1)
Rabbit
(11)
All Species
Showing Publications 1 - 10 of 48. Show All 48 Publications.
Publications using NB600-450 Applications Species
huynh t, Reed C, Blackwell Z et al. Local IL-10 delivery modulates the immune response and enhances repair of volumetric muscle loss muscle injury Research Square 2022-08-05 (IHC-Fr, Rat) IHC-Fr Rat
Gundogdu G, Okhunov Z, Starek S et al. Evaluation of Bi-Layer Silk Fibroin Grafts for Penile Tunica Albuginea Repair in a Rabbit Corporoplasty Model Frontiers in bioengineering and biotechnology 2021-12-07 [PMID: 34950646] (IHC, Rabbit) IHC Rabbit
Struijk C, Van Genechten W, Verdonk P et al. Human meniscus allograft augmentation by allogeneic mesenchymal stromal/stem cell injections Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2021-05-10 [PMID: 33969529]
Wei Q, Kong N, Liu X et al. Pirfenidone attenuates synovial fibrosis and postpones the progression of osteoarthritis by anti-fibrotic and anti-inflammatory properties in vivo and in vitro Journal of translational medicine 2021-04-19 [PMID: 33874948] (IHC, Rabbit) IHC Rabbit
Marvin K, Schwartz I, Utz E et al. Effects of Fractional CO2 Laser Treatment on Subglottic Scar in a Rabbit Model Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery 2020-12-08 [PMID: 33287672] (IHC, Rabbit) IHC Rabbit
Oikawa K, Teixeira LBC, Keikhosravi A et al. Microstructure and resident cell-types of the feline optic nerve head resemble that of humans Exp Eye Res 2020-10-19 [PMID: 33091431] (ICC/IF, Feline) ICC/IF Feline
Huynh T, Kim J, Dunlap G et al. In-vivo testing of an injectable matrix gel for the treatment of shoulder cuff muscle fatty degeneration Journal of Shoulder and Elbow Surgery 2020-06-01 [PMID: 32713662] (IHC, IHC-Fr, Rabbit, Rat) IHC, IHC-Fr Rabbit, Rat
Limraksasin P, Okawa H, Zhang M et al. Size-Optimized Microspace Culture Facilitates Differentiation of Mouse Induced Pluripotent Stem Cells into Osteoid-Rich Bone Constructs Stem Cells International 2020-05-14 [PMID: 32508932] (IHC-P, Mouse) IHC-P Mouse
Zwaans Bmm, Wegner Ka, Bartolone Sn et Al. Radiation cystitis modeling: A comparative study of bladder fibrosis radio-sensitivity in C57BL/6, C3H, and BALB/c mice Physiol Rep 2020-02-01 [PMID: 32109348] (ICC/IF, Mouse) ICC/IF Mouse
Rybinska I, Sandri M, Bianchi F et al. Extracellular Matrix Features Discriminate Aggressive HER2-Positive Breast Cancer Patients Who Benefit from Trastuzumab Treatment Cells 2020-02-13 [PMID: 32069815] (IHC-Fr, Human) IHC-Fr Human
Show All 48 Publications.

Reviews for Collagen I alpha 1 Antibody (NB600-450) (5) 4.65

Average Rating: 4.6
(Based on 5 reviews)
We have 5 reviews tested in 5 species: Mouse, Feline, Goat, Other, Pig.

Reviews using NB600-450:
Filter by Applications
IHC-P
(1)
Flow
(1)
IHC-Fr
(1)
ICC
(1)
IF
(1)
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Filter by Species
Mouse
(1)
Feline
(1)
Goat
(1)
Other
(1)
Pig
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Collagen I alpha 1 NB600-450
Enlarge
4
reviewed by:
Mozhdeh Sojoodi
IHC-P Mouse 06/24/2021
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedLiver
SpeciesMouse
LotNB600-450

Comments

CommentsPrimary antibody dilution 1:200
Enzymatic/proteolytic antigen retrieval
No Permeabilization
Flow Cytometry Collagen I alpha 1 NB600-450
Enlarge
5
reviewed by:
Clément CHARROYER
Flow Goat 01/22/2019
View

Summary

ApplicationFlow Cytometry
Sample TestedChondrocyte
SpeciesGoat
Lot067M4805V
  4
reviewed by:
Kazuya Oikawa
IHC-Fr Feline 09/15/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSclera
SpeciesFeline
Lot067M4805V
Immunocytochemistry Collagen I alpha 1 NB600-450
Enlarge
5
reviewed by:
L P
ICC Pig 06/07/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedPig trabecular meshwork cells
SpeciesPig
LotN/A
Immunofluorescence Collagen I alpha 1 NB600-450
Enlarge
5
reviewed by:
Verified Customer
IF Other 04/21/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedEquine Tenocytes
SpeciesOther

Product General Protocols

View specific protocols for Collagen I alpha 1 Antibody (NB600-450): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Collagen I alpha 1 Antibody (NB600-450). (Showing 1 - 5 of 5 FAQs).

  1. We are looking for an anti-Collagen antibody for Flow Cytometry (FACS) analysis in Swine sample. Would you please provide some references if there is any available as well?
    • We currently have one antibody for porcine Collagen validated in flow cytometry (catalog # NBP1-94202).
  2. I am looking for anti collagen 1 antibody, I see your product calls it collagen I alpha 1 is there a difference?
    • Collagen I alpha 1 is the most common protein for the collagen 1 protein. There are other subunits as well (such as collagen 1 alpha 2…etc.).
  3. The predicted molecular weight says 150kDA but the band on the gel appears at 130kDA why is that?
    • Differences between the predicted and observed MW can be affected by a number of factors including PTMs, relative charges and experimental factors. These cumulative effect of these differences can be quite substantial with larger molecules such as collagen I alpha 1.
  4. How long does delivery take for your collagen 1 alpha 1 antibody?
    • Our most popular collagen I alpha 1 antibody (NB600-408) is usually in stock and ships priority overnight for next business day delivery in the U.S.
  5. What is the dilution factor for IHC for starting? Because the range of dilution factor on the datasheet is so broad, I am confused which dilution factor is suitable for starting.
    • I can recommend the following dilution to start. Keep in mind that these will all vary greatly by sample type, so these are just suggestions. You may need to use more or less antibody. For catalog number NB600-450, the dilution range is 1:1000-1:2000.

Secondary Antibodies

 

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Recent Reviews

4.6
5
3
4
2
3
0
2
0
1
0

Mozhdeh Sojoodi
06/24/2021
Application: IHC-P
Species: Mouse

Clément CHARROYER
01/22/2019
Application: Flow
Species: Goat

Kazuya Oikawa
09/15/2018
Application: IHC-Fr
Species: Feline

Bioinformatics

Gene Symbol COL1A1
Entrez