Collagen I alpha 1 Antibody (COL-1)


Western Blot: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Analysis of collagen 1. Lane 1: 3 ug Recombinant human collagen 1. Lane 2: 20 ug pig skin whole cell lysate extracted with laemmli buffer.
Immunocytochemistry/ Immunofluorescence: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - analysis of Collagen I alpha 1 in 4% PFA fixed equine tenocytes using anti-Collagen I alpha 1 antibody. Image from verified more
Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Analysis of formalin fixed frozen porcine skin tissue sections. Blocking: 5% serum for 1 hr at 20C. Sample was incubated for 16 hours at 4C and more
Immunohistochemistry-Frozen: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Collagen I Antibody (COL-1) [NB600-450] - Staining of acetone-fixed, frozen sections of pig tongue at a dilution of 1:4000
Immunohistochemistry-Frozen: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - taining of acetone-fixed, frozen sections of human tongue at a dilution of 1:2000
Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Analysis of a formalin/pfa fixed paraffin embedded ligament section. Enzymatic antigen retrieval and blocking using 1% BSA for 30 min at 20C was more

Product Details

Reactivity Hu, Mu, Rt, Po, Bv, Eq, Ma, RbSpecies Glossary
Applications WB, DB, ELISA, IHC, IHC-Fr, IHC-P
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

Collagen I alpha 1 Antibody (COL-1) Summary

Full length bovine native protein (purified).
The epitope recognized by the antibody may be sensitive to routine formalin fixation and paraffin embedding.
Extracellular matrix
Recognizes the native (helical) form of collagen type I using ELISA and dot-blot. Does not react with the thermally-denatured molecule. The shows no cross-reactivity with collagen types II, III, IV, V, VI, VII, IX, X and XI.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
15mM Sodium Azide


  • Western Blot 1:100-1:2000
  • Dot Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:2000
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
The predicted molecular weight in western blot is 95 kDa. In immunohistochemical testing of acetone-fixed or unfixed frozen sections, strong staining of connective tissue fibers is seen. This antibody has been used in immunohistochemistry as reported in the literature (PMID: 22019120) Immunohistochemistry-Paraffin was reported in scientific literature. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24242003)
Theoretical MW
139 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
NB600-450 in the following applications:

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NB600-450 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25287675). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Alternate Names for Collagen I alpha 1 Antibody (COL-1)

  • Alpha-1 type I collagen
  • COL1A1
  • Collagen 1 alpha 1
  • Collagen 1
  • collagen alpha 1 chain type I
  • collagen alpha-1(I) chain
  • collagen of skin, tendon and bone, alpha-1 chain
  • collagen, type I, alpha 1
  • Collagen1
  • Collagen-1
  • OI4
  • pro-alpha-1 collagen type 1


The extracellular matrix is the material found in the extracellular environment of all tissues and organs. It consists of basement membranes and interstitial stroma. The composition of the extracellular framework of all vertebrates is dominated by a class of molecules known as collagens,1 each with unique features suited for its function and location. The collagens are proteins composed of three subunit polypeptides that can vary in length, which interact to form a triple helix. The molecular basis of the triple helix is provided by a repeated unique amino acid sequence (Gly-x-y) and the polypeptides generated are capable of assembly into fibrillar or other types of supramolecular assemblies which are deposited in the extracellular matrix. The number of distinct collagen types presently recognized is more than fourteen.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Collagen I alpha 1 Antibody (NB600-450)(8)

We have publications tested in 4 confirmed species: Human, Mouse, Equine, Rabbit.

We have publications tested in 3 applications: ICC/IF, IHC, IHC-P.

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Showing Publications 1 - 8 of 8.
Publications using NB600-450 Applications Species
Cheng MQ, Wahafu T, Jiang GF et al. A novel open-porous magnesium scaffold with controllable microstructures and properties for bone regeneration. Sci Rep. 2016 Apr 13 [PMID:27071777] (IHC-P, Human) IHC-P Human
Geburek F, Lietzau M, Beineke A et al. Effect of a single injection of autologous conditioned serum (ACS) on tendon healing in equine naturally occurring tendinopathies Stem Cell Res Ther 2015 Jul 24 [PMID:26113022] (IHC-P, Equine) IHC-P Equine
Otani Y, Komura M, Komura H et al. Optimal amount of basic fibroblast growth factor (b-FGF) in gelatin sponges incorporating B-tricalcium phosphate (TCP) with chondrocytes. Tissue Eng Part A. 2014 Oct 06 [PMID:25287675] (IHC, Mouse) IHC Mouse
Triulzi T, Ratti M, Tortoreto M et al. Maspin influences response to doxorubicin by changing the tumor microenvironment organization. Int J Cancer. 2013 Nov 14 [PMID:24242003] (IHC-P, Human) IHC-P Human
You W, Fan L, Duan D et al. Foxc2 over-expression in bone marrow mesenchymal stem cells stimulates osteogenic differentiation and inhibits adipogenic differentiation. Mol Cell Biochem. 2013 Oct 12 [PMID:24122419] (ICC/IF, Rabbit) ICC/IF Rabbit
Murali S, Rai B, Dombrowski C et al. Affinity-selected heparan sulfate for bone repair. Biomaterials 2013 Apr 27 [PMID:23632323] (IHC-P, Rabbit) IHC-P Rabbit
Re’em T, Kaminer-Israeli Y, Ruvinov E, Cohen S. Chondrogenesis of hMSC in affinity-bound TGF-beta scaffolds. Biomaterials. 2011 Oct 21. [PMID:22019120]
Krasinski Z, Biskupski P, Dzieciuchowicz L et al. The influence of elastic components of the venous wall on the biomechanical properties of different veins used for arterial reconstruction. Eur J Vasc Endovasc Surg 2010 Aug [PMID:20570188] (IHC, Human) IHC Human

Reviews for Collagen I alpha 1 Antibody (NB600-450) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Other.

Reviews using NB600-450:
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Images Ratings Applications Species Date Details
 Collagen I alpha 1 NB600-450
reviewed by:
Other 04/21/2015


Sample TestedEquine Tenocytes


Blocking Details5% BSA in 1X PBS, 20 minutes, room temperature

Primary Anitbody

Dilution Ratio1:2000, 2 hours, room temperature

Secondary Antibody

Secondary DescriptionGoat Anti-Mouse IgG, Alexa Fluor 594
Secondary Manufacturer Cat#A11005
Secondary Concentration1:500 dilution


Detection NotesHT-1080 (positive control), Human PBMCs (negative control)
Fixation Details4% PFA fix for 20 minutes at room temperature; 30 minute permeabilization with 0.01% Tween 20
Wash Description1X PBS, 5 minutes, 3 washes

Product General Protocols

View specific protocols for Collagen I alpha 1 Antibody (NB600-450): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Collagen I alpha 1 Antibody (NB600-450). (Showing 1 - 9 of 9 FAQs).

  1. We are looking for an anti-Collagen antibody for Flow Cytometry (FACS) analysis in Swine sample. Would you please provide some references if there is any available as well?
    • We currently have one antibody for porcine Collagen validated in flow cytometry (catalog # NBP1-94202).
  2. I am looking for anti collagen 1 antibody, I see your product calls it collagen I alpha 1 is there a difference?
    • Collagen I alpha 1 is the most common protein for the collagen 1 protein. There are other subunits as well (such as collagen 1 alpha 2…etc.).
  3. Can this collagen 1 alpha 1 antibody be conjugated?
    • Unfortunately we do not offer this particular collagen 1 alpha 1 antibody in a directly conjugated form; however you may want to consider one of our other collagen I alpha 1 antibodies such as NBP1-77458 that is offered in several conjugated forms.
  4. What is the epitope for this collagen I alpha 1 antibody? I need to make sure that there won’t be cross reactivity with other collagens.
    • This collagen I alpha 1 antibody has not been epitope mapped; however, it has been shown there is less than 1% cross reactivity between other collagen forms and this collagen I alpha 1 antibody.
  5. What is the immunogen for this collagen antibody?
    • Pepsin digested type 1 collagen from human and bovine placenta was used as the immunogen for this collagen I alpha 1 antibody.
  6. The predicted molecular weight says 150kDA but the band on the gel appears at 130kDA why is that?
    • Differences between the predicted and observed MW can be affected by a number of factors including PTMs, relative charges and experimental factors. These cumulative effect of these differences can be quite substantial with larger molecules such as collagen I alpha 1.
  7. How long does delivery take for your collagen 1 alpha 1 antibody?
    • Our most popular collagen I alpha 1 antibody (NB600-408) is usually in stock and ships priority overnight for next business day delivery in the U.S.
  8. What secondary antibody do you recommend for collagen I alpha 1 antibodies?
    • Because this collagen I alpha 1 antibody is raised in rabbit you would want to use any anti-rabbit IgG secondary antibody. NB7160 is a popular choice for use in WB.
  9. What is the dilution factor for IHC for starting? Because the range of dilution factor on the datasheet is so broad, I am confused which dilution factor is suitable for starting.
    • I can recommend the following dilution to start. Keep in mind that these will all vary greatly by sample type, so these are just suggestions. You may need to use more or less antibody. For catalog number NB600-450, the dilution range is 1:1000-1:2000.

Secondary Antibodies


Isotype Controls

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Contact Information

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Gene Symbol COL1A1

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