Collagen I alpha 1 Antibody (COL-1)


Western Blot: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Analysis of collagen 1. Lane 1: 3 ug Recombinant human collagen 1. Lane 2: 20 ug pig skin whole cell lysate extracted with laemmli buffer.
Immunohistochemistry-Frozen: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Staining of acetone-fixed, frozen sections of pig tongue at a dilution of 1:4000
Immunocytochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Collagen I alpha1 labeled pig trabecular meshwork cells indirectly visualized with Alexa488 conjugated secondary antibody. This image was submitted more
Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Analysis of a formalin/pfa fixed paraffin embedded ligament section. Enzymatic antigen retrieval and blocking using 1% BSA for 30 min at 20C was more
Immunohistochemistry-Frozen: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Staining of acetone-fixed, frozen sections of human tongue at a dilution of 1:2000
Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Analysis of formalin fixed frozen porcine skin tissue sections. Blocking: 5% serum for 1 hr at 20C. Sample was incubated for 16 hours at 4C and more
Immunocytochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Analysis of Collagen I alpha 1 in 4% PFA fixed equine tenocytes using anti-Collagen I alpha 1 antibody. Image from verified customer review.

Product Details

Reactivity Hu, Mu, Rt, Po, Bv, Eq, Ma, RbSpecies Glossary
Applications WB, DB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, ICC/IF

Order Details

Collagen I alpha 1 Antibody (COL-1) Summary

Full length bovine native protein (purified).
The epitope recognized by the antibody may be sensitive to routine formalin fixation and paraffin embedding.
Extracellular matrix
Recognizes the native (helical) form of collagen type I using ELISA and dot-blot. Does not react with the thermally-denatured molecule. The shows no cross-reactivity with collagen types II, III, IV, V, VI, VII, IX, X and XI.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:100-1:2000
  • Dot Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:2000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunocytochemistry
Application Notes
The predicted molecular weight in western blot is 95 kDa. In immunohistochemical testing of acetone-fixed or unfixed frozen sections, strong staining of connective tissue fibers is seen. This antibody has been used in IHC as reported in the literature (PMID: 22019120) including IHC-Paraffin (PMID 24242003) and ICC (PMID 27769912).
Theoretical MW
139 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
NB600-450 in the following applications:

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NB600-450 in the following applications:

Reactivity Notes

Also reported reactivity in deer. Mouse reactivity reported in scientific literature (PMID: 25287675). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
15mM Sodium Azide

Alternate Names for Collagen I alpha 1 Antibody (COL-1)

  • Alpha-1 type I collagen
  • COL1A1
  • Collagen 1 alpha 1
  • Collagen 1
  • collagen alpha 1 chain type I
  • collagen alpha-1(I) chain
  • Collagen I alpha 1
  • collagen of skin, tendon and bone, alpha-1 chain
  • collagen, type I, alpha 1
  • Collagen1
  • Collagen-1
  • OI4
  • pro-alpha-1 collagen type 1


The extracellular matrix is the material found in the extracellular environment of all tissues and organs. It consists of basement membranes and interstitial stroma. The composition of the extracellular framework of all vertebrates is dominated by a class of molecules known as collagens,1 each with unique features suited for its function and location. The collagens are proteins composed of three subunit polypeptides that can vary in length, which interact to form a triple helix. The molecular basis of the triple helix is provided by a repeated unique amino acid sequence (Gly-x-y) and the polypeptides generated are capable of assembly into fibrillar or other types of supramolecular assemblies which are deposited in the extracellular matrix. The number of distinct collagen types presently recognized is more than fourteen.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Collagen I alpha 1 Antibody (NB600-450)(16)

We have publications tested in 4 confirmed species: Human, Mouse, Equine, Rabbit.

We have publications tested in 4 applications: ELISA, ICC/IF, IHC, IHC-P.

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Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB600-450 Applications Species
Kuzan A, Chwilkowska A, Maksymowicz K et al. Advanced glycation end products as a source of artifacts in immunoenzymatic methods Glycoconj. J. 2018 Jan 05 [PMID: 29305778] (ELISA, Human) ELISA Human
Ramani K, Tomasi ML, Berlind J et al. Role of A-Kinase Anchoring Protein Phosphorylation in Alcohol-Induced Liver Injury and Hepatic Stellate Cell Activation Am. J. Pathol. 2018 Jan 02 [PMID: 29305319] (Human) Human
Wegner K, Keikhosravi A, Eliceiri K et al. Fluorescence of Picrosirius Red Multiplexed With Immunohistochemistry for the Quantitative Assessment of Collagen in Tissue Sections. J Histochem Cytochem 2017 Jul 10 [PMID: 28692327] (IHC-P, Mouse) IHC-P Mouse
da Silva IR, Tiveron LCRDC, da Silva MV et al. In Situ Cytokine Expression and Morphometric Evaluation of Total Collagen and Collagens Type I and Type III in Keloid Scars. Mediators Inflamm. 2017 Jun 22 [PMID: 28638180] (IHC, Human) IHC Human
Bono N, Meghezi S, Soncini M et al. A Dual-Mode Bioreactor System for Tissue Engineered Vascular Models. Ann Biomed Eng. 2017 Feb 21 [PMID: 28224370]
Sa G, Xiong X, Ren J et al. Expression of fibrosis-related molecules in the oral mucosa of six animal species: A reference for selecting animal models. Eur J Pharm Sci Oct 18 2016 [PMID: 27769912] (IHC, ICC/IF, Human) IHC, ICC/IF Human
Karti O, Zengin MO, Cinar E et al. Effect of 1- and 6-Hour-Delayed Corneal Collagen Cross-Linking on Corneal Healing in a Rabbit Alkali-Burn Model: Clinical and Histological Observations. Cornea. Oct 12 2016 [PMID: 27741014]
Loy C, Laine A, Mantovani D. Rotation-based technique for the rapid densification of tubular collagen gel scaffolds. Biotechnol J 2016 Dec [PMID: 27731544]
Cheng MQ, Wahafu T, Jiang GF et al. A novel open-porous magnesium scaffold with controllable microstructures and properties for bone regeneration. Sci Rep. 2016 Apr 13 [PMID: 27071777] (IHC-P, Human) IHC-P Human
Geburek F, Lietzau M, Beineke A et al. Effect of a single injection of autologous conditioned serum (ACS) on tendon healing in equine naturally occurring tendinopathies Stem Cell Res Ther 2015 Jul 24 [PMID: 26113022] (IHC-P, Equine)

Collagen I alpha 1 /COL 1A1 antibody was used for IHC-P analysis of longitudinal sections of tendon biopsies from superficial digital flexor tendons with tendinopathy from horses that were subjected or not to single injection of autologous conditioned serum (ACS).
IHC-P Equine
Show All 16 Publications.

Review for Collagen I alpha 1 Antibody (NB600-450) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NB600-450:
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Images Ratings Applications Species Date Details
Immunofluorescence Collagen I alpha 1 NB600-450
reviewed by:
Jessica Prieto
IF Other 04/21/2015


Sample TestedEquine Tenocytes


Blocking Details5% BSA in 1X PBS, 20 minutes, room temperature

Primary Anitbody

Dilution Ratio1:2000, 2 hours, room temperature

Secondary Antibody

Secondary DescriptionGoat Anti-Mouse IgG, Alexa Fluor 594
Secondary Manufacturer Cat#A11005
Secondary Concentration1:500 dilution


Detection NotesHT-1080 (positive control), Human PBMCs (negative control)
Fixation Details4% PFA fix for 20 minutes at room temperature; 30 minute permeabilization with 0.01% Tween 20
Wash Description1X PBS, 5 minutes, 3 washes

Product General Protocols

View specific protocols for Collagen I alpha 1 Antibody (NB600-450): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Collagen I alpha 1 Antibody (NB600-450). (Showing 1 - 9 of 9 FAQs).

  1. We are looking for an anti-Collagen antibody for Flow Cytometry (FACS) analysis in Swine sample. Would you please provide some references if there is any available as well?
    • We currently have one antibody for porcine Collagen validated in flow cytometry (catalog # NBP1-94202).
  2. I am looking for anti collagen 1 antibody, I see your product calls it collagen I alpha 1 is there a difference?
    • Collagen I alpha 1 is the most common protein for the collagen 1 protein. There are other subunits as well (such as collagen 1 alpha 2…etc.).
  3. Can this collagen 1 alpha 1 antibody be conjugated?
    • Unfortunately we do not offer this particular collagen 1 alpha 1 antibody in a directly conjugated form; however you may want to consider one of our other collagen I alpha 1 antibodies such as NBP1-77458 that is offered in several conjugated forms.
  4. What is the epitope for this collagen I alpha 1 antibody? I need to make sure that there won’t be cross reactivity with other collagens.
    • This collagen I alpha 1 antibody has not been epitope mapped; however, it has been shown there is less than 1% cross reactivity between other collagen forms and this collagen I alpha 1 antibody.
  5. What is the immunogen for this collagen antibody?
    • Pepsin digested type 1 collagen from human and bovine placenta was used as the immunogen for this collagen I alpha 1 antibody.
  6. The predicted molecular weight says 150kDA but the band on the gel appears at 130kDA why is that?
    • Differences between the predicted and observed MW can be affected by a number of factors including PTMs, relative charges and experimental factors. These cumulative effect of these differences can be quite substantial with larger molecules such as collagen I alpha 1.
  7. How long does delivery take for your collagen 1 alpha 1 antibody?
    • Our most popular collagen I alpha 1 antibody (NB600-408) is usually in stock and ships priority overnight for next business day delivery in the U.S.
  8. What secondary antibody do you recommend for collagen I alpha 1 antibodies?
    • Because this collagen I alpha 1 antibody is raised in rabbit you would want to use any anti-rabbit IgG secondary antibody. NB7160 is a popular choice for use in WB.
  9. What is the dilution factor for IHC for starting? Because the range of dilution factor on the datasheet is so broad, I am confused which dilution factor is suitable for starting.
    • I can recommend the following dilution to start. Keep in mind that these will all vary greatly by sample type, so these are just suggestions. You may need to use more or less antibody. For catalog number NB600-450, the dilution range is 1:1000-1:2000.

Secondary Antibodies


Isotype Controls

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Recent Reviews


Jessica Prieto
Application: IF
Species: Other


Gene Symbol COL1A1