Collagen I alpha 1 Antibody (COL-1)

Immunohistochemistry-Frozen: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Staining of acetone-fixed, frozen sections of pig tongue at a dilution of 1:4000

Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Analysis of an FFPE ligament section. Enzymatic antigen retrieval and blocking using 1% BSA for 30 min at 20C was performed. Sample was incubated for 1 hour with primary antibody at a dilution of 1:400 at 20C.

Immunocytochemistry/Immunofluorescence: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Collagen I and collagen III accumulate in C57BL/6 mice in response to radiation. Bladder strips from irradiated and control mice were stained for collagen I or for collagen III (NB600-594, RRID:AB_10001330) and imaged using fluorescent microscopy. Representative images (100 x 100 um) of each mouse strain and treatment group are provided for collagen I and collagen III immunofluorescence. Image collected and cropped by CiteAb from the following publication (www.onlinelibrary.wiley.com/doi/abs/10.14814/phy2.14377) licensed under a CC-BY licence.

Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Staining of acetone-fixed, frozen sections of human tongue at a dilution of 1:2000.

Immunocytochemistry/Immunofluorescence: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Analysis of Collagen I alpha 1 in 4% PFA fixed equine tenocytes using anti-Collagen I alpha 1 antibody. ICC/IF image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Collagen I alpha1 labeled pig trabecular meshwork cells indirectly visualized with Alexa Fluor 488 conjugated secondary antibody. ICC/IF image submitted by a verified customer review.

Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Analysis of formalin fixed frozen porcine skin tissue sections. Blocking: 5% serum for 1 hr at 20C. Sample was incubated for 16 hours at 4C and used at a dilution of 1:500.

Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Immunohistochemistry for type I collagen in keloid (20x). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28638180/) licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Mouse healthy liver. Anibody at 1:200. Enzymatic/proteolytic antigen retrieval. No permeabilization. IHC-P image submitted by a verified customer review.

• Reactivity: Hu, Mu, Rt, Po, Bv, Eq, Fe, Ma, Rb
• Applications: WB, DB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
• Clone: COL-1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Collagen I alpha 1 Antibody (COL-1) Summary

• Immunogen: This Collagen I alpha 1 antibody (COL-1) was raised against full length bovine native protein (purified).
• Epitope: The epitope recognized by the antibody may be sensitive to routine formalin fixation and paraffin embedding.
• Localization: Extracellular matrix
• Specificity: This Collagen I alpha 1 antibody (COL-1) recognizes the native (helical) form of collagen type I using ELISA and dot-blot. It does not react with the thermally-denatured molecule, and shows no cross-reactivity with collagen types II, III, IV, V, VI, VII, IX, X and XI.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: COL1A1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Dot Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:2000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
• Theoretical MW: 139 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Reported reactivity in deer. Mouse reactivity reported in scientific literature (PMID: 25287675). Goat reactivity reported by customer review. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions. Feline reactivity reported in scientific literature (PMID:33091431).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 15mM Sodium Azide
• Purity: Unpurified

Alternate Names for Collagen I alpha 1 Antibody (COL-1)

• Alpha-1 type I collagen
• COL1A1
• COL-IA1
• Collagen 1 alpha 1
• Collagen 1
• collagen alpha 1 chain type I
• collagen alpha-1(I) chain
• Collagen I alpha 1
• collagen of skin, tendon and bone, alpha-1 chain
• Collagen Type I Alpha 1 Chain
• collagen, type I, alpha 1
• Collagen1
• Collagen-1
• EDSARTH1
• OI4
• pro-alpha-1 collagen type 1
• Type I Procollagen Alpha 1 Chain

Background

Collagen is an extracellular matrix protein that serves as a scaffold defining the shape and mechanical properties of many tissues and organs including skin, tendon, artery walls, fibrocartilage, bone and teeth. Collagens are highly conserved and are characterized by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. The extensive family of collagens is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagen type I (also known as collagen alpha, COL1A1, and alpha-1 type I collagen) is the largest component of fibrillar collagen found in cartilage and connective tissues. It is synthesized by fibroblasts, osteoblasts, and odontoblasts and has a theoretical molecular weight of 138 kDa.

Type I collagen is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon tissue. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue. Several collagens play a role in cell adhesion, responsible for maintaining normal tissue architecture and function. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Post-Golgi LH3 trafficking is essential for collagen homeostasis and for the development and function of multiple organs and tissues (1).

The COL1A1 gene encodes the pro-alpha1 chains of type I collagen protein, whose triple helix is comprised of two alpha1 chains and one alpha2 chain. Mutations in the encoding COL1A1 gene are associated with brittle bone disease (Osteogenesis Imperfecta), cortical hyperostosis (Caffey disease) and disorders that affect the connective tissues (Ehlers-Danlos syndrome) (2). Studies have found that HIF-1 transcription regulation of collagen prolyl hydroxylases regulates collagen deposition, promoting cancer cell alignment along collagen fibers, which enhances invasion and metastasis to lymph nodes and lung tissue by breast cancer cells (3).

References

1. Banushi, B., Forneris, F., Straatman-Iwanowska, A., Strange, A., Lyne, A. M., Rogerson, C., . . . Gissen, P. (2016). Regulation of post-Golgi LH3 trafficking is essential for collagen homeostasis. Nat Commun, 7, 12111. doi:10.1038/ncomms12111

2. Lu, Y., Zhang, S., Wang, Y., Ren, X., & Han, J. (2019). Molecular mechanisms and clinical manifestations of rare genetic disorders associated with type I collagen. Intractable Rare Dis Res, 8(2), 98-107. doi:10.5582/irdr.2019.01064

3. Gilkes, D. M., Chaturvedi, P., Bajpai, S., Wong, C. C., Wei, H., Pitcairn, S., . . . Semenza, G. L. (2013). Collagen prolyl hydroxylases are essential for breast cancer metastasis. Cancer Res, 73(11), 3285-3296. doi:10.1158/0008-5472.Can-12-3963

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Collagen I alpha 1 Antibody (NB600-450)(48)

Publications using NB600-450

• huynh t, Reed C, Blackwell Z et al. Local IL-10 delivery modulates the immune response and enhances repair of volumetric muscle loss muscle injury Research Square 2022-08-05 (IHC-Fr, Rat)
• Gundogdu G, Okhunov Z, Starek S et al. Evaluation of Bi-Layer Silk Fibroin Grafts for Penile Tunica Albuginea Repair in a Rabbit Corporoplasty Model Frontiers in bioengineering and biotechnology 2021-12-07 [PMID: 34950646] (IHC, Rabbit)
• Struijk C, Van Genechten W, Verdonk P et al. Human meniscus allograft augmentation by allogeneic mesenchymal stromal/stem cell injections Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2021-05-10 [PMID: 33969529]
• Wei Q, Kong N, Liu X et al. Pirfenidone attenuates synovial fibrosis and postpones the progression of osteoarthritis by anti-fibrotic and anti-inflammatory properties in vivo and in vitro Journal of translational medicine 2021-04-19 [PMID: 33874948] (IHC, Rabbit)
• Marvin K, Schwartz I, Utz E et al. Effects of Fractional CO2 Laser Treatment on Subglottic Scar in a Rabbit Model Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery 2020-12-08 [PMID: 33287672] (IHC, Rabbit)
• Oikawa K, Teixeira LBC, Keikhosravi A et al. Microstructure and resident cell-types of the feline optic nerve head resemble that of humans Exp Eye Res 2020-10-19 [PMID: 33091431] (ICC/IF, Feline)
• Huynh T, Kim J, Dunlap G et al. In-vivo testing of an injectable matrix gel for the treatment of shoulder cuff muscle fatty degeneration Journal of Shoulder and Elbow Surgery 2020-06-01 [PMID: 32713662] (IHC, IHC-Fr, Rabbit, Rat)
• Limraksasin P, Okawa H, Zhang M et al. Size-Optimized Microspace Culture Facilitates Differentiation of Mouse Induced Pluripotent Stem Cells into Osteoid-Rich Bone Constructs Stem Cells International 2020-05-14 [PMID: 32508932] (IHC-P, Mouse)
• Zwaans Bmm, Wegner Ka, Bartolone Sn et Al. Radiation cystitis modeling: A comparative study of bladder fibrosis radio-sensitivity in C57BL/6, C3H, and BALB/c mice Physiol Rep 2020-02-01 [PMID: 32109348] (ICC/IF, Mouse)
• Rybinska I, Sandri M, Bianchi F et al. Extracellular Matrix Features Discriminate Aggressive HER2-Positive Breast Cancer Patients Who Benefit from Trastuzumab Treatment Cells 2020-02-13 [PMID: 32069815] (IHC-Fr, Human)
• Rauf S, Qazi H, Luo J et al. Real-time, highly sensitive detection of alpha-fetoprotein in biological fluids using a QCM sensor based on a Cu2O@MoS2-Au nanocomposite and gold staining IEEE Sensors Journal 2021-12-10
• RINCHIUSO A Interaction of an easy-to-use tissue-engineered collagen-based model for in vitro stent evaluation Thesis
• Li H, Zhao T, Cao F et al. Integrated bioactive scaffold with aptamer targeted stem cell recruitment and growth factor induced pro differentiation effects for anisotropic meniscal regeneration Bioengineering & Translational Medicine 2022-03-03 (IHC-P, Rabbit)
• McClellan P, Ina JG, Knapik DM et al. Mesenchymal Stem Cell Delivery via Topographically Tenoinductive Collagen Biotextile Enhances Regeneration of Segmental Tendon Defects The American journal of sports medicine 2022-07-01 [PMID: 35647785]
• Gan Z, Zhao Y, Wu Y et al. Three-dimensional, biomimetic electrospun scaffolds reinforced with carbon nanotubes for temporomandibular joint disc regeneration Acta biomaterialia 2022-05-10 [PMID: 35562008] (ICC/IF, Rat)
• Verma SK, Yaghoobi H, Slaine P Et al. Multi-pin contact drawing enables production of anisotropic collagen fiber substrates for alignment of fibroblasts and monocytes Colloids Surf B Biointerfaces 2022-05-02 [PMID: 35500531] (Rat)
  
  Details:
  Citation using the Janelia Fluor 549 version of this antibody.
• Kim JT, Roberts K, Dunlap G et al. Nandrolone supplementation does not improve functional recovery in an aged animal model of volumetric muscle loss injury Journal of tissue engineering and regenerative medicine 2022-02-03 [PMID: 35113494] (IHC, Rat)
• Meng X, Grad S, Wen C et al. An impaired healing model of osteochondral defect in papain-induced arthritis J Orthop Translat 2021-01-13 [PMID: 33437629]
• Wang Y, Xiao Y, Long S et al. Gasdermin D in macrophages restrains colitis by controlling cGAS-mediated inflammation Sci Adv 2020-07-17 [PMID: 32671214]
• Liu J, Yu C, Chen Y et al. Fast fabrication of stable cartilage-like tissue using collagen hydrogel microsphere culture J Mater Chem B 2020-04-09 [PMID: 32264594]
• Yu C, Liu J, Lu G et al. Repair of osteochondral defects in a rabbit model with artificial cartilage particulates derived from cultured collagen-chondrocyte microspheres. J Mater Chem B 2020-04-08 [PMID: 32254543]
• Limraksasin P, Kondo T, Zhang M et al. In Vitro Fabrication of Hybrid Bone/Cartilage Complex Using Mouse Induced Pluripotent Stem Cells Int J Mol Sci 2020-01-16 [PMID: 31963264] (IHC, Mouse)
• Willemse J, Verstegen M, Vermeulen A et al. Fast, robust and effective decellularization of whole human livers using mild detergents and pressure controlled perfusion Materials Science and Engineering: C 2019-09-12 [PMID: 31923991] (IHC, IHC-P, Human)
• Robbach BP, Gulecyuz MF, Kempfert L, et al. Rotator Cuff Repair With Autologous Tenocytes and Biodegradable Collagen Scaffold: A Histological and Biomechanical Study in Sheep Am J Sports Med 2019-12-16 [PMID: 31841352]
• Chen M, Feng Z, Guo W et al. PCL-MECM-Based Hydrogel Hybrid Scaffolds and Meniscal Fibrochondrocytes Promote Whole Meniscus Regeneration in a Rabbit Meniscectomy Model ACS Appl Mater Interfaces 2019-10-22 [PMID: 31596568]
• Jallow F, O'Leary KA, Rugowski DE et al. Dynamic interactions between the extracellular matrix and estrogen activity in progression of ER+ breast cancer Oncogene 2019-08-12 [PMID: 31406251]
• Ligresti G, Caporarello N, Meridew JA et al. Status of growth plates can be monitored by MRI J Magn Reson Imaging 2019-05-01 [PMID: 31044458] (IHC, Rabbit)
• Wu D, Wang Z, Wang J et al. Development of a micro-tissue-mediated injectable bone tissue engineering strategy for large segmental bone defect treatment Stem Cell Res Ther 2018-11-28 [PMID: 30486863] (WB, Rabbit)
• Learn G D, McClellan P E et al. Woven collagen biotextiles enable mechanically functional rotator cuff tendon regeneration during repair of segmental tendon defects in vivo. J Biomed Mater Res B Appl Biomater 2019-01-08 [PMID: 30485649] (IHC, Rabbit)
• Costa D, Vene R, Benelli R et al. Targeting the Epidermal Growth Factor Receptor Can Counteract the Inhibition of Natural Killer Cell Function Exerted by Colorectal Tumor-Associated Fibroblasts Front Immunol 2018-05-29 [PMID: 29910806] (Human)
• Kuzan A, Chwilkowska A, Maksymowicz K et al. Advanced glycation end products as a source of artifacts in immunoenzymatic methods Glycoconj. J. 2018-01-05 [PMID: 29305778] (ELISA, Human)
• Ramani K, Tomasi ML, Berlind J et al. Role of A-Kinase Anchoring Protein Phosphorylation in Alcohol-Induced Liver Injury and Hepatic Stellate Cell Activation Am. J. Pathol. 2018-01-02 [PMID: 29305319] (Human)
• Liu G Y, Agarwal R, Ko K R et al. Templated Assembly of Collagen Fibers Directs Cell Growth in 2D and 3D. Scientific Reports 2017-01-01 [PMID: 28852121] (IHC, Mouse)
• Wegner K, Keikhosravi A, Eliceiri K et al. Fluorescence of Picrosirius Red Multiplexed With Immunohistochemistry for the Quantitative Assessment of Collagen in Tissue Sections. J Histochem Cytochem 2017-07-10 [PMID: 28692327] (IHC-P, Mouse)
• da Silva IR, Tiveron LCRDC, da Silva MV et al. In Situ Cytokine Expression and Morphometric Evaluation of Total Collagen and Collagens Type I and Type III in Keloid Scars. Mediators Inflamm. 2017-06-22 [PMID: 28638180] (IHC, Human)
• Du Y, Liu H, Yang Q et al. Selective laser sintering scaffold with hierarchical architecture and gradient composition for osteochondral repair in rabbits. Biomaterials. 2017-08-01 [PMID: 28528301] (IHC, Rabbit)
• Bono N, Meghezi S, Soncini M et al. A Dual-Mode Bioreactor System for Tissue Engineered Vascular Models. Ann Biomed Eng. 2017-02-21 [PMID: 28224370]
• Sa G, Xiong X, Ren J et al. Expression of fibrosis-related molecules in the oral mucosa of six animal species: A reference for selecting animal models. Eur J Pharm Sci 2016-10-18 [PMID: 27769912] (ICC/IF, IHC, Human)
• Karti O, Zengin MO, Cinar E et al. Effect of 1- and 6-Hour-Delayed Corneal Collagen Cross-Linking on Corneal Healing in a Rabbit Alkali-Burn Model: Clinical and Histological Observations. Cornea. 2016-10-12 [PMID: 27741014]
• Loy C, Laine A, Mantovani D. Rotation-based technique for the rapid densification of tubular collagen gel scaffolds. Biotechnol J 2016-12-01 [PMID: 27731544]
• Cheng MQ, Wahafu T, Jiang GF et al. A novel open-porous magnesium scaffold with controllable microstructures and properties for bone regeneration. Sci Rep. 2016-04-13 [PMID: 27071777] (IHC-P, Human)
• Geburek F, Lietzau M, Beineke A et al. Effect of a single injection of autologous conditioned serum (ACS) on tendon healing in equine naturally occurring tendinopathies Stem Cell Res Ther 2015-07-24 [PMID: 26113022] (IHC-P, Equine)
  
  Details:
  Collagen I alpha 1 /COL 1A1 antibody was used for IHC-P analysis of longitudinal sections of tendon biopsies from superficial digital flexor tendons with tendinopathy from horses that were subjected or not to single injection of autologous conditioned serum (ACS).
• Otani Y, Komura M, Komura H et al. Optimal amount of basic fibroblast growth factor (b-FGF) in gelatin sponges incorporating B-tricalcium phosphate (TCP) with chondrocytes. Tissue Eng Part A. 2014-10-06 [PMID: 25287675] (IHC, Mouse)
• Triulzi T, Ratti M, Tortoreto M et al. Maspin influences response to doxorubicin by changing the tumor microenvironment organization. Int J Cancer. 2013-11-14 [PMID: 24242003] (IHC-P, Human)
• You W, Fan L, Duan D et al. Foxc2 over-expression in bone marrow mesenchymal stem cells stimulates osteogenic differentiation and inhibits adipogenic differentiation. Mol Cell Biochem. 2013-10-12 [PMID: 24122419] (ICC/IF, Rabbit)
• Murali S, Rai B, Dombrowski C et al. Affinity-selected heparan sulfate for bone repair. Biomaterials 2013-04-27 [PMID: 23632323] (IHC-P, Rabbit)
• Re’em T, Kaminer-Israeli Y, Ruvinov E, Cohen S. Chondrogenesis of hMSC in affinity-bound TGF-beta scaffolds. Biomaterials. 2011-10-21 [PMID: 22019120]
• Krasinski Z, Biskupski P, Dzieciuchowicz L et al. The influence of elastic components of the venous wall on the biomechanical properties of different veins used for arterial reconstruction. Eur J Vasc Endovasc Surg 2010-08-01 [PMID: 20570188] (IHC, Human)

Reviews for Collagen I alpha 1 Antibody (NB600-450) (5)

Average Rating: 4.6

(Based on 5 reviews)

We have 5 reviews tested in 5 species: Mouse, Feline, Goat, Other, Pig.

reviewed by: Mozhdeh Sojoodi IHC-P Mouse 06/24/2021

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Liver
• Species: Mouse
• Lot: NB600-450

Comments

• Comments: Primary antibody dilution 1:200
  Enzymatic/proteolytic antigen retrieval
  No Permeabilization

reviewed by: Clément CHARROYER Flow Goat 01/22/2019

Summary

• Application: Flow Cytometry
• Sample Tested: Chondrocyte
• Species: Goat
• Lot: 067M4805V

reviewed by: Kazuya Oikawa IHC-Fr Feline 09/15/2018

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Sclera
• Species: Feline
• Lot: 067M4805V

reviewed by: L P ICC Pig 06/07/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Pig trabecular meshwork cells
• Species: Pig
• Lot: N/A

reviewed by: Verified Customer IF Other 04/21/2015

Summary

• Application: Immunofluorescence
• Sample Tested: Equine Tenocytes
• Species: Other

FAQs for Collagen I alpha 1 Antibody (NB600-450). (Showing 1 - 5 of 5 FAQs).

1. We are looking for an anti-Collagen antibody for Flow Cytometry (FACS) analysis in Swine sample. Would you please provide some references if there is any available as well?
   • We currently have one antibody for porcine Collagen validated in flow cytometry (catalog # NBP1-94202).
2. I am looking for anti collagen 1 antibody, I see your product calls it collagen I alpha 1 is there a difference?
   • Collagen I alpha 1 is the most common protein for the collagen 1 protein. There are other subunits as well (such as collagen 1 alpha 2…etc.).
3. The predicted molecular weight says 150kDA but the band on the gel appears at 130kDA why is that?
   • Differences between the predicted and observed MW can be affected by a number of factors including PTMs, relative charges and experimental factors. These cumulative effect of these differences can be quite substantial with larger molecules such as collagen I alpha 1.
4. How long does delivery take for your collagen 1 alpha 1 antibody?
   • Our most popular collagen I alpha 1 antibody (NB600-408) is usually in stock and ships priority overnight for next business day delivery in the U.S.
5. What is the dilution factor for IHC for starting? Because the range of dilution factor on the datasheet is so broad, I am confused which dilution factor is suitable for starting.
   • I can recommend the following dilution to start. Keep in mind that these will all vary greatly by sample type, so these are just suggestions. You may need to use more or less antibody. For catalog number NB600-450, the dilution range is 1:1000-1:2000.

Bioinformatics

• Gene Symbol: COL1A1
• Entrez:
  • 1277(Human)
  • 29393(Rat)