Collagen I alpha 1 Antibody (COL-1)

Images

 
Western Blot: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Analysis of collagen 1. Lane 1: Recombinant human collagen 1 (3 ug). Lane 2: Pig skin whole cell lysate (20 ug) extracted with Laemmli buffer.
Immunohistochemistry-Frozen: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Staining of acetone-fixed, frozen sections of pig tongue at a dilution of 1:4000
Immunocytochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Collagen I alpha1 labeled pig trabecular meshwork cells indirectly visualized with Alexa Fluor 488 conjugated secondary antibody. Image from ...read more
Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Analysis of an FFPE ligament section. Enzymatic antigen retrieval and blocking using 1% BSA for 30 min at 20C was performed. Sample was incubated ...read more
Immunohistochemistry-Frozen: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Staining of acetone-fixed, frozen sections of human tongue at a dilution of 1:2000.
Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Analysis of formalin fixed frozen porcine skin tissue sections. Blocking: 5% serum for 1 hr at 20C. Sample was incubated for 16 hours at 4C and ...read more
Immunocytochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Analysis of Collagen I alpha 1 in 4% PFA fixed equine tenocytes using anti-Collagen I alpha 1 antibody. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Eq, Fe, Ma, RbSpecies Glossary
Applications WB, DB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, ICC/IF
Clone
COL-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Collagen I alpha 1 Antibody (COL-1) Summary

Immunogen
Full length bovine native protein (purified).
Epitope
The epitope recognized by the antibody may be sensitive to routine formalin fixation and paraffin embedding.
Localization
Extracellular matrix
Specificity
Recognizes the native (helical) form of collagen type I using ELISA and dot-blot. Does not react with the thermally-denatured molecule. The shows no cross-reactivity with collagen types II, III, IV, V, VI, VII, IX, X and XI.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
COL1A1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Dot Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:2000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunocytochemistry
Application Notes
The predicted molecular weight in Western blot is 95 kDa. In IHC testing of acetone-fixed or unfixed frozen sections, strong staining of connective tissue fibers is seen. This antibody has been used in IHC as reported in the literature (PMID: 22019120) including IHC-Paraffin (PMID 24242003) and ICC (PMID 27769912). Use in Flow Cytometry reported by customer review.
Theoretical MW
139 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 4 Reviews rated 4.8
using
NB600-450 in the following applications:

Publications
Read Publications using
NB600-450 in the following applications:

Reactivity Notes

Also reported reactivity in deer. Mouse reactivity reported in scientific literature (PMID: 25287675). Goat reactivity reported by customer review. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
15mM Sodium Azide
Purity
Unpurified

Alternate Names for Collagen I alpha 1 Antibody (COL-1)

  • Alpha-1 type I collagen
  • COL1A1
  • Collagen 1 alpha 1
  • Collagen 1
  • collagen alpha 1 chain type I
  • collagen alpha-1(I) chain
  • Collagen I alpha 1
  • collagen of skin, tendon and bone, alpha-1 chain
  • collagen, type I, alpha 1
  • Collagen1
  • Collagen-1
  • OI4
  • pro-alpha-1 collagen type 1

Background

The extracellular matrix is the material found in the extracellular environment of all tissues and organs. It consists of basement membranes and interstitial stroma. The composition of the extracellular framework of all vertebrates is dominated by a class of molecules known as collagens,1 each with unique features suited for its function and location. The collagens are proteins composed of three subunit polypeptides that can vary in length, which interact to form a triple helix. The molecular basis of the triple helix is provided by a repeated unique amino acid sequence (Gly-x-y) and the polypeptides generated are capable of assembly into fibrillar or other types of supramolecular assemblies which are deposited in the extracellular matrix. The number of distinct collagen types presently recognized is more than fourteen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-58529
Species: Hu
Applications: ICC/IF
NB600-594
Species: Hu, Mu, Rt, Bv, Ma
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
MAB1419
Species: Hu, Rt
Applications: IHC, CyTOF-ready, ICC, ICFlow
NB600-844
Species: Hu, Mu, Rt, Ch, Rb
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-20460
Species: Hu, Mu, Rt
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP
NBP2-01223
Species: Hu
Applications: WB, Flow, IHC, IHC-P
NBP1-77461
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-89953
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
NB300-164
Species: Hu, Po, Ca, Pm, Rt(-)
Applications: WB, ICC/IF, IHC-Fr, IHC-P, IP
NBP1-91258
Species: Hu, Mu, Po, Bv, Ca, Eq, Fe
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
AF808
Species: Mu
Applications: WB, IHC, ELISA(Cap), ELISA(Det), ICC, Neut, ELISA(Sta)
7270-IL/CF
Species: Hu
NB100-724
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC
NBP2-35212
Species: Hu
NB100-74350
Species: Hu, Mu, Rt, Po, Bv, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB600-450
Species: Hu, Mu, Rt, Po, Bv, Eq, Fe, Ma, Rb
Applications: WB, DB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, ICC/IF

Publications for Collagen I alpha 1 Antibody (NB600-450)(20)

We have publications tested in 4 confirmed species: Human, Mouse, Equine, Rabbit.

We have publications tested in 5 applications: ELISA, ICC/IF, IHC, IHC-P, WB.


Filter By Application
ELISA
(1)
ICC/IF
(2)
IHC
(6)
IHC-P
(5)
WB
(1)
All Applications
Filter By Species
Human
(8)
Mouse
(3)
Equine
(1)
Rabbit
(4)
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using NB600-450 Applications Species
Wu D, Wang Z, Wang J et al. Development of a micro-tissue-mediated injectable bone tissue engineering strategy for large segmental bone defect treatment Stem Cell Res Ther Nov 28 2018 [PMID: 30486863] (WB, Rabbit) WB Rabbit
Costa D, Vene R, Benelli R et al. Targeting the Epidermal Growth Factor Receptor Can Counteract the Inhibition of Natural Killer Cell Function Exerted by Colorectal Tumor-Associated Fibroblasts Front Immunol May 29 2018 [PMID: 29910806] (Human) Human
Kuzan A, Chwilkowska A, Maksymowicz K et al. Advanced glycation end products as a source of artifacts in immunoenzymatic methods Glycoconj. J. 2018 Jan 05 [PMID: 29305778] (ELISA, Human) ELISA Human
Ramani K, Tomasi ML, Berlind J et al. Role of A-Kinase Anchoring Protein Phosphorylation in Alcohol-Induced Liver Injury and Hepatic Stellate Cell Activation Am. J. Pathol. 2018 Jan 02 [PMID: 29305319] (Human) Human
Liu G Y, Agarwal R, Ko K R et al. Templated Assembly of Collagen Fibers Directs Cell Growth in 2D and 3D. Scientific Reports 2017 [PMID: 28852121] (IHC, Mouse) IHC Mouse
Wegner K, Keikhosravi A, Eliceiri K et al. Fluorescence of Picrosirius Red Multiplexed With Immunohistochemistry for the Quantitative Assessment of Collagen in Tissue Sections. J Histochem Cytochem 2017 Jul 10 [PMID: 28692327] (IHC-P, Mouse) IHC-P Mouse
da Silva IR, Tiveron LCRDC, da Silva MV et al. In Situ Cytokine Expression and Morphometric Evaluation of Total Collagen and Collagens Type I and Type III in Keloid Scars. Mediators Inflamm. 2017 Jun 22 [PMID: 28638180] (IHC, Human) IHC Human
Du Y, Liu H, Yang Q et al. Selective laser sintering scaffold with hierarchical architecture and gradient composition for osteochondral repair in rabbits. Biomaterials. Aug 1 2017 [PMID: 28528301] (IHC, Rabbit) IHC Rabbit
Bono N, Meghezi S, Soncini M et al. A Dual-Mode Bioreactor System for Tissue Engineered Vascular Models. Ann Biomed Eng. 2017 Feb 21 [PMID: 28224370]
Sa G, Xiong X, Ren J et al. Expression of fibrosis-related molecules in the oral mucosa of six animal species: A reference for selecting animal models. Eur J Pharm Sci Oct 18 2016 [PMID: 27769912] (IHC, ICC/IF, Human) IHC, ICC/IF Human
Show All 20 Publications.

Reviews for Collagen I alpha 1 Antibody (NB600-450) (4) 4.84

Average Rating: 4.8
(Based on 4 reviews)
We have 4 reviews tested in 4 species: Feline, Goat, Other, Pig.

Reviews using NB600-450:
Filter by Applications
Flow
(1)
IHC-Fr
(1)
ICC
(1)
IF
(1)
All Applications
Filter by Species
Feline
(1)
Goat
(1)
Other
(1)
Pig
(1)
All Species
Images Ratings Applications Species Date Details
Flow Cytometry Collagen I alpha 1 NB600-450
Enlarge
reviewed by:
Anonymous
Flow Goat 01/22/2019
View

Summary

ApplicationFlow Cytometry
Sample TestedChondrocyte
SpeciesGoat
Lot067M4805V
Immunohistochemistry-Frozen Collagen I alpha 1 NB600-450
Enlarge
reviewed by:
Anonymous
IHC-Fr Feline 09/15/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSclera
SpeciesFeline
Lot067M4805V
Immunocytochemistry Collagen I alpha 1 NB600-450
Enlarge
reviewed by:
Anonymous
ICC Pig 06/07/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedPig trabecular meshwork cells
SpeciesPig
LotN/A
Immunofluorescence Collagen I alpha 1 NB600-450
Enlarge
reviewed by:
Jessica Prieto
IF Other 04/21/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedEquine Tenocytes
SpeciesOther

Blocking

Blocking Details5% BSA in 1X PBS, 20 minutes, room temperature

Primary Anitbody

Dilution Ratio1:2000, 2 hours, room temperature

Secondary Antibody

Secondary DescriptionGoat Anti-Mouse IgG, Alexa Fluor 594
Secondary Manufacturer Cat#A11005
Secondary Concentration1:500 dilution

Details

Detection NotesHT-1080 (positive control), Human PBMCs (negative control)
Fixation Details4% PFA fix for 20 minutes at room temperature; 30 minute permeabilization with 0.01% Tween 20
Wash Description1X PBS, 5 minutes, 3 washes

Product General Protocols

View specific protocols for Collagen I alpha 1 Antibody (NB600-450): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Collagen I alpha 1 Antibody (NB600-450). (Showing 1 - 9 of 9 FAQs).

  1. We are looking for an anti-Collagen antibody for Flow Cytometry (FACS) analysis in Swine sample. Would you please provide some references if there is any available as well?
    • We currently have one antibody for porcine Collagen validated in flow cytometry (catalog # NBP1-94202).
  2. I am looking for anti collagen 1 antibody, I see your product calls it collagen I alpha 1 is there a difference?
    • Collagen I alpha 1 is the most common protein for the collagen 1 protein. There are other subunits as well (such as collagen 1 alpha 2…etc.).
  3. Can this collagen 1 alpha 1 antibody be conjugated?
    • Unfortunately we do not offer this particular collagen 1 alpha 1 antibody in a directly conjugated form; however you may want to consider one of our other collagen I alpha 1 antibodies such as NBP1-77458 that is offered in several conjugated forms.
  4. What is the epitope for this collagen I alpha 1 antibody? I need to make sure that there won’t be cross reactivity with other collagens.
    • This collagen I alpha 1 antibody has not been epitope mapped; however, it has been shown there is less than 1% cross reactivity between other collagen forms and this collagen I alpha 1 antibody.
  5. What is the immunogen for this collagen antibody?
    • Pepsin digested type 1 collagen from human and bovine placenta was used as the immunogen for this collagen I alpha 1 antibody.
  6. The predicted molecular weight says 150kDA but the band on the gel appears at 130kDA why is that?
    • Differences between the predicted and observed MW can be affected by a number of factors including PTMs, relative charges and experimental factors. These cumulative effect of these differences can be quite substantial with larger molecules such as collagen I alpha 1.
  7. How long does delivery take for your collagen 1 alpha 1 antibody?
    • Our most popular collagen I alpha 1 antibody (NB600-408) is usually in stock and ships priority overnight for next business day delivery in the U.S.
  8. What secondary antibody do you recommend for collagen I alpha 1 antibodies?
    • Because this collagen I alpha 1 antibody is raised in rabbit you would want to use any anti-rabbit IgG secondary antibody. NB7160 is a popular choice for use in WB.
  9. What is the dilution factor for IHC for starting? Because the range of dilution factor on the datasheet is so broad, I am confused which dilution factor is suitable for starting.
    • I can recommend the following dilution to start. Keep in mind that these will all vary greatly by sample type, so these are just suggestions. You may need to use more or less antibody. For catalog number NB600-450, the dilution range is 1:1000-1:2000.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for Collagen I alpha 1 Antibody (NB600-450)

Discover related pathways, diseases and genes to Collagen I alpha 1 Antibody (NB600-450). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Collagen I alpha 1 Antibody (NB600-450)

Discover more about diseases related to Collagen I alpha 1 Antibody (NB600-450).
 

Pathways for Collagen I alpha 1 Antibody (NB600-450)

View related products by pathway.

PTMs for Collagen I alpha 1 Antibody (NB600-450)

Learn more about PTMs related to Collagen I alpha 1 Antibody (NB600-450).
 

Research Areas for Collagen I alpha 1 Antibody (NB600-450)

Find related products by research area.

Blogs on Collagen I alpha 1

There are no specific blogs for Collagen I alpha 1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.8
5
3
4
1
3
0
2
0
1
0

Anonymous
01/22/2019
Application: Flow
Species: Goat

Anonymous
09/15/2018
Application: IHC-Fr
Species: Feline

Anonymous
06/07/2017
Application: ICC
Species: Pig

Bioinformatics

Gene Symbol COL1A1
Entrez