CD44 Antibody (IM7.8.1R) [Unconjugated]

Human peripheral blood mononuclear cells (PBMCs) were stained with Rat Anti-Human/Mouse CD44 Monoclonal Antibody (Catalog # MAB6127, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Mouse splenocytes were stained with Rat Anti-Human/Mouse CD44 Monoclonal Antibody (Catalog # MAB6127, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

• Reactivity: Hu, Mu
• Applications: Flow, CyTOF-ready
• Clone: IM7.8.1R
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

CD44 Antibody (IM7.8.1R) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Myeloid leukemia M1 cells induced with Dexamethasone
• Specificity: Detects human and mouse CD44 in flow cytometry.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rat
• Gene: CD44
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-reported
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD44 Antibody (IM7.8.1R) [Unconjugated]

• CD44 antigen
• CD44 molecule (Indian blood group)
• CD44
• CD44R
• CDw44
• cell surface glycoprotein CD44
• chondroitin sulfate proteoglycan 8
• CSPG8
• ECMR-III
• epican
• Extracellular matrix receptor III
• GP90 lymphocyte homing/adhesion receptor
• HCAM
• HCELL
• hematopoietic cell E- and L-selectin ligand
• Heparan sulfate proteoglycan
• Hermes antigen
• homing function and Indian blood group system
• HUTCH-I
• Hyaluronate receptor
• IN
• LHR
• MC56
• MDU2
• MDU2CD44 antigen (homing function and Indian blood group system)
• MDU3
• MDU3CDW44
• MIC4
• MIC4MGC10468
• MUTCH-I
• Pgp1
• PGP-1
• PGP-I
• Phagocytic glycoprotein 1
• Phagocytic glycoprotein I

Background

CD44, also known as ECMR-III, Hermes, Pgp1, Ly-24, GP90, or HUTCH, is a ubiquitously expressed protein that is the major receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Mouse CD44 has a 22 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan-binding disulfide-stabilized link region and a 48-463 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. CD44 exists as a large number of different isoforms due to alternative RNA splicing. Clone IM7.8.1 has been reported to recognize all isoforms of CD44.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD44 Antibody (MAB6127)(7)

Publications using MAB6127

• Xidan Zhu, Jia Feng, Wenguang Fu, Xiaojia Shu, Xue Wan, Jinbo Liu Effects of cisplatin on the proliferation, invasion and apoptosis of breast cancer cells following beta ‑catenin silencing International Journal of Molecular Medicine 3/16/2020 [PMID: 32186756]
• Jun Wei, Jin Sun, Yu Liu Enhanced targeting of prostate cancer‑initiating cells by salinomycin‑encapsulated lipid‑PLGA nanoparticles linked with CD44 antibodies Oncology Letters 2/19/2019 [PMID: 30930999]
• F Yang, Z Zheng, L Zheng, J Qin, H Li, X Xue, J Gao, G Fang siRNA-encapsulated immunoliposomes conjugated with CD44 antibodies target and eliminate gastric cancer-initiating cells Onco Targets Ther, 2018-10-11;11(0):6811-6825. 2018-10-11 [PMID: 30349314] (Complex Application, Human)
• KR Wiehagen, NM Girgis, DH Yamada, AA Smith, SR Chan, IS Grewal, M Quigley, RI Verona Combination of CD40 agonism and CSF-1R blockade reconditions tumor-associated macrophages and drives potent antitumor immunity Cancer Immunol Res, 2017-11-02;0(0):. 2017-11-02 [PMID: 29097420] (Flow Cytometry, Mouse)
• J Jung, LJY Kim, X Wang, Q Wu, T Sanvoranar, CG Hubert, BC Prager, LC Wallace, X Jin, SC Mack, JN Rich Nicotinamide metabolism regulates glioblastoma stem cell maintenance JCI Insight, 2017-05-18;2(10):. 2017-05-18 [PMID: 28515364] (Western Blot, Human)
• Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke Stem Cells Int, 2016-10-20;2016(0):6104780. 2016-10-20 [PMID: 27840648] (Flow Cytometry, Human)
• Kashima Y, Takahashi M, Shiba Y, Itano N, Izawa A, Koyama J, Nakayama J, Taniguchi S, Kimata K, Ikeda U Crucial role of hyaluronan in neointimal formation after vascular injury. PLoS ONE, 2013-03-06;8(3):e58760. 2013-03-06 [PMID: 23484050] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: CD44