CD4 Antibody [Biotin]

CD4 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Canine CD4 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2410) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Ca
• Applications: WB, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

CD4 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant canine CD4
  Val25-Lys401
  Accession # P33705
• Specificity: Detects canine CD4 in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant feline CD4 is observed, 5% cross‑reactivity with recombinant human CD4 is observed, and less than 1% cross-reactivity with recombinant mouse CD4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CD4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD4 Antibody [Biotin]

• CD_antigen: CD4
• CD4 antigen (p55)
• CD4 antigen
• CD4 molecule
• CD4 receptor
• CD4
• CD4mut
• T-cell surface antigen T4/Leu-3
• T-cell surface glycoprotein CD4

Background

CD4 is a type I membrane protein belonging to the immunoglobulin superfamily. It functions as an accessory protein for T cell receptor/MHC class II antigen interaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for CD4 Antibody (BAF2410). (Showing 1 - 1 of 1 FAQ).

1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
   • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

Bioinformatics

• Gene Symbol: CD4
• Uniprot:
  • P33705()