CD37 Antibody (WR17) - Chimeric - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: CD37 Antibody (WR17) [NBP2-81048] - Immunofluorescence staining of fixed Daudi cells with anti-CD37 antibody (WR17) [NBP2-81048]. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of WR17 [NBP2-81048] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81048, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu
• Applications: Flow, ICC/IF, IHC
• Clone: WR17
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

CD37 Antibody (WR17) - Chimeric - Azide and BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody. Clone WR17 was used by HLDA to establish CD designation.
• Immunogen: Chronic lymphocytic leukaemia cells.
• Specificity: Recognises human CD37.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CD37
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
• Application Notes: This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG2a format, for improved compatibility with existing reagents, assays and techniques. This antibody binds CD37, staining, amongst others, B-cells and B-cell tumours.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CD37 Antibody (WR17) - Chimeric - Azide and BSA Free

• CD37 antigentetraspanin-26
• CD37 molecule
• CD37
• cell differentiation antigen 37
• GP52-40
• leukocyte antigen CD37
• MGC120234
• Tetraspanin-26
• TSPAN26
• tspan-26
• TSPAN26leukocyte surface antigen CD37

Background

CD37, also known as GP52-40, TSPAN26, MGC120234. Enterz Protein NP_001035120. It is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It may play a role in T-cell-B-cell interactions. Alternate splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD37