Neprilysin/CD10 Antibody [Unconjugated]

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Neprilysin/CD10 was detected in perfusion fixed frozen sections of mouse brain (glial cell in hippocampus) using 15 µg/mL Goat Anti-Mouse Neprilysin/CD10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1126) ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, IP, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Neprilysin/CD10 Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Neprilysin/CD10
Tyr52-Trp750
Accession # AAA37386
Specificity
Detects mouse Neprilysin/CD10 in ELISAs and Western blots. In sandwich immunoassays, less than 35% cross-reactivity with recombinant human (rh) Neprilysin is observed and less than 0.2% cross-reactivity with rhNeprilysin-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MME
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Mouse Neprilysin/CD10 Antibody (Catalog # AF1126)
ELISA Detection: Mouse Neprilysin/CD10 Biotinylated Antibody (Catalog # BAF1126)
Standard: Recombinant Mouse Neprilysin/CD10 (Catalog # 1126-ZN)
Publications
Read Publications using
AF1126 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neprilysin/CD10 Antibody [Unconjugated]

  • Atriopeptidase
  • CALLA
  • CALLAmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase)
  • CD10 antigen
  • CD10
  • CD10)
  • CD10membrane metallo-endopeptidase variant 1
  • Common acute lymphocytic leukemia antigen
  • DKFZp686O16152
  • EC 3.4.24
  • EC 3.4.24.11
  • Enkephalinase
  • EPN
  • Leu-19
  • membrane metallo-endopeptidase variant 2
  • membrane metallo-endopeptidase
  • MGC126681
  • MGC126707
  • MME
  • NEPmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA
  • Neprilysin
  • Neutral Endopeptidase 24.11
  • Neutral endopeptidase
  • NKH1
  • SFE
  • Skin fibroblast elastase

Background

Neprilysin (NEP, neutral endopeptidase 24.11, EC 3.4.24.11) is a zinc metallopeptidase expressed at the cell surface of a variety of cells. The enzyme functions both as an endopeptidase with a thermolysin-like specificity and as a dipeptidylcarboxypeptidase. NEP has been shown to be involved in the degradation of enkephalins in the mammalian brain and the inactivation of circulating atrial natriuretic peptide (1, 2). NEP has also been identified as the common acute lymphoblastic leukemia antigen (CALLA), and to be expressed on the surface of lymphocytes in some disease states (3). These and other observations have resulted in considerable clinical interest in NEP as a potential target for analgesics and antihypertensive drugs. NEP is also a major degrading enzyme of amyloid beta peptide (A beta ) in the brain, indicating that down-regulation of NEP activity, which could be caused by aging, can contribute to the development of Alzheimer’s disease by promoting A beta accumulation (4).

  1. Malfroy, B. et al. (1978) Nature 276:523.
  2. Kenny, A.J. and S.L. Stephenson (1988) FEBS Lett. 232:1.
  3. LeTarte, M. et al. (1988) J. Exp. Med. 168:1247.
  4. Itwata, N. et al. (2001) Science 292:1550.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Neprilysin/CD10 Antibody (AF1126)(7)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: ELISA Capture, IHC, IHC-Fr, Western Blot.


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ELISA Capture
(1)
IHC
(1)
IHC-Fr
(1)
Western Blot
(4)
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Mouse
(7)
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Showing Publications 1 - 7 of 7.
Publications using AF1126 Applications Species
L Wang, Y Tang, AF Buckley, RF Spurney Blockade of the natriuretic peptide clearance receptor attenuates proteinuria in a mouse model of focal segmental glomerulosclerosis Physiological Reports, 2021;9(21):e15095. 2021 [PMID: 34755480] (Western Blot, Mouse) Western Blot Mouse
A Thanabalas, AJ Chiang, C Morehouse, M Camara, S Hawkins, AE Keller, AC Koksal, CS Caceres, AA Berlin, N Holoweckyj, VN Takahashi, L Cheng, M de Los Rey, M Pelletier, AC Patera, B Sellman, S Hess, M Marelli, CC Boo, TS Cohen, A DiGiandome PD-L1+ neutrophils contribute to injury-induced infection susceptibility Science Advances, 2021;7(10):. 2021 [PMID: 33674305] (ELISA Capture, Mouse) ELISA Capture Mouse
SH Jeon, N Kim, YJ Ju, MS Gee, D Lee, JK Lee Phytohormone Abscisic Acid Improves Memory Impairment and Reduces Neuroinflammation in 5xFAD Mice by Upregulation of LanC-Like Protein 2 Int J Mol Sci, 2020;21(22):. 2020 [PMID: 33182586] (Western Blot, Mouse) Western Blot Mouse
Alawi L, Emberesh S, Owuor B, Chodavarapu H, Fadnavis R, El-Amouri S, Elased K Effect of hyperglycemia and rosiglitazone on renal and urinary neprilysin in db/db diabetic mice. Physiol Rep, 0;8(3):e14364. 0 [PMID: 32026607] (IHC, Mouse) IHC Mouse
K Kidana, T Tatebe, K Ito, N Hara, A Kakita, T Saito, S Takatori, Y Ouchi, T Ikeuchi, M Makino, TC Saido, M Akishita, T Iwatsubo, Y Hori, T Tomita Loss of kallikrein-related peptidase 7 exacerbates amyloid pathology in Alzheimer's disease model mice EMBO Mol Med, 2018;0(0):. 2018 [PMID: 29311134] (IHC-Fr, Mouse) IHC-Fr Mouse
Sagare A, Bell R, Zhao Z, Ma Q, Winkler E, Ramanathan A, Zlokovic B Pericyte loss influences Alzheimer-like neurodegeneration in mice. Nat Commun, 2013;4(0):2932. 2013 [PMID: 24336108] (Western Blot, Mouse) Western Blot Mouse
Liu Y, Studzinski C, Beckett T, Guan H, Hersh MA, Murphy MP, Klein R, Hersh LB Expression of neprilysin in skeletal muscle reduces amyloid burden in a transgenic mouse model of Alzheimer disease. Mol. Ther., 2009;17(8):1381-6. 2009 [PMID: 19471248] (Western Blot, Mouse) Western Blot Mouse

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Bioinformatics

Gene Symbol MME
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