Neprilysin/CD10 Antibody [Unconjugated]

Neprilysin/CD10 was detected in perfusion fixed frozen sections of mouse brain (glial cell in hippocampus) using 15 µg/mL Goat Anti-Mouse Neprilysin/CD10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1126) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC, IP, ELISA(Cap)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Neprilysin/CD10 Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Neprilysin/CD10
  Tyr52-Trp750
  Accession # AAA37386
• Specificity: Detects mouse Neprilysin/CD10 in ELISAs and Western blots. In sandwich immunoassays, less than 35% cross-reactivity with recombinant human (rh) Neprilysin is observed and less than 0.2% cross-reactivity with rhNeprilysin-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MME
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse Neprilysin/CD10 Antibody (Catalog # AF1126)
  ELISA Detection: Mouse Neprilysin/CD10 Biotinylated Antibody (Catalog # BAF1126)
  Standard: Recombinant Mouse Neprilysin/CD10 (Catalog # 1126-ZN)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neprilysin/CD10 Antibody [Unconjugated]

• Atriopeptidase
• CALLA
• CALLAmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase)
• CD10 antigen
• CD10
• CD10)
• CD10membrane metallo-endopeptidase variant 1
• Common acute lymphocytic leukemia antigen
• DKFZp686O16152
• EC 3.4.24
• EC 3.4.24.11
• Enkephalinase
• EPN
• Leu-19
• membrane metallo-endopeptidase variant 2
• membrane metallo-endopeptidase
• MGC126681
• MGC126707
• MME
• NEPmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA
• Neprilysin
• Neutral Endopeptidase 24.11
• Neutral endopeptidase
• NKH1
• SFE
• Skin fibroblast elastase

Background

Neprilysin (NEP, neutral endopeptidase 24.11, EC 3.4.24.11) is a zinc metallopeptidase expressed at the cell surface of a variety of cells. The enzyme functions both as an endopeptidase with a thermolysin-like specificity and as a dipeptidylcarboxypeptidase. NEP has been shown to be involved in the degradation of enkephalins in the mammalian brain and the inactivation of circulating atrial natriuretic peptide (1, 2). NEP has also been identified as the common acute lymphoblastic leukemia antigen (CALLA), and to be expressed on the surface of lymphocytes in some disease states (3). These and other observations have resulted in considerable clinical interest in NEP as a potential target for analgesics and antihypertensive drugs. NEP is also a major degrading enzyme of amyloid beta peptide (A beta ) in the brain, indicating that down-regulation of NEP activity, which could be caused by aging, can contribute to the development of Alzheimer’s disease by promoting A beta accumulation (4).

1. Malfroy, B. et al. (1978) Nature 276:523.
2. Kenny, A.J. and S.L. Stephenson (1988) FEBS Lett. 232:1.
3. LeTarte, M. et al. (1988) J. Exp. Med. 168:1247.
4. Itwata, N. et al. (2001) Science 292:1550.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neprilysin/CD10 Antibody (AF1126)(8)

Publications using AF1126

• C Qu, QP Li, ZR Su, SP Ip, QJ Yuan, YL Xie, QQ Xu, W Yang, YF Huang, YF Xian, ZX Lin Nano-Honokiol ameliorates the cognitive deficits in TgCRND8 mice of Alzheimer's disease via inhibiting neuropathology and modulating gut microbiota Journal of advanced research, 2022;35(0):231-243. 2022-01-01 [PMID: 35024199] (Western Blot, Rat)
• L Wang, Y Tang, AF Buckley, RF Spurney Blockade of the natriuretic peptide clearance receptor attenuates proteinuria in a mouse model of focal segmental glomerulosclerosis Physiological Reports, 2021;9(21):e15095. 2021-01-01 [PMID: 34755480] (Western Blot, Mouse)
• A Thanabalas, AJ Chiang, C Morehouse, M Camara, S Hawkins, AE Keller, AC Koksal, CS Caceres, AA Berlin, N Holoweckyj, VN Takahashi, L Cheng, M de Los Rey, M Pelletier, AC Patera, B Sellman, S Hess, M Marelli, CC Boo, TS Cohen, A DiGiandome PD-L1+ neutrophils contribute to injury-induced infection susceptibility Science Advances, 2021;7(10):. 2021-01-01 [PMID: 33674305] (ELISA Capture, Mouse)
• SH Jeon, N Kim, YJ Ju, MS Gee, D Lee, JK Lee Phytohormone Abscisic Acid Improves Memory Impairment and Reduces Neuroinflammation in 5xFAD Mice by Upregulation of LanC-Like Protein 2 Int J Mol Sci, 2020;21(22):. 2020-01-01 [PMID: 33182586] (Western Blot, Mouse)
• Alawi L, Emberesh S, Owuor B, Chodavarapu H, Fadnavis R, El-Amouri S, Elased K Effect of hyperglycemia and rosiglitazone on renal and urinary neprilysin in db/db diabetic mice. Physiol Rep, 0;8(3):e14364. [PMID: 32026607] (IHC, Mouse)
• K Kidana, T Tatebe, K Ito, N Hara, A Kakita, T Saito, S Takatori, Y Ouchi, T Ikeuchi, M Makino, TC Saido, M Akishita, T Iwatsubo, Y Hori, T Tomita Loss of kallikrein-related peptidase 7 exacerbates amyloid pathology in Alzheimer's disease model mice EMBO Mol Med, 2018;0(0):. 2018-01-01 [PMID: 29311134] (IHC-Fr, Mouse)
• Sagare A, Bell R, Zhao Z, Ma Q, Winkler E, Ramanathan A, Zlokovic B Pericyte loss influences Alzheimer-like neurodegeneration in mice. Nat Commun, 2013;4(0):2932. 2013-01-01 [PMID: 24336108] (Western Blot, Mouse)
• Liu Y, Studzinski C, Beckett T, Guan H, Hersh MA, Murphy MP, Klein R, Hersh LB Expression of neprilysin in skeletal muscle reduces amyloid burden in a transgenic mouse model of Alzheimer disease. Mol. Ther., 2009;17(8):1381-6. 2009-01-01 [PMID: 19471248] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: MME
• Uniprot:
  • AAA37386()