Neprilysin/CD10 Antibody [Unconjugated]

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Neprilysin/CD10 was detected in perfusion fixed frozen sections of mouse brain (glial cell in hippocampus) using 15 µg/mL Goat Anti-Mouse Neprilysin/CD10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1126) ...read more
Astrocyte‐specific Stat3 deletion increases microglial A beta internalization and degradation, and reduces apoE expression, dystrophic neurites, and detrimental cytokinesAInternalization of A beta (stained with IC16 ...read more
Astrocyte‐specific Stat3 deletion increases microglial A beta internalization and degradation, and reduces apoE expression, dystrophic neurites, and detrimental cytokinesAInternalization of A beta (stained with IC16 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, IP, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Neprilysin/CD10 Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Neprilysin/CD10
Tyr52-Trp750
Accession # AAA37386
Specificity
Detects mouse Neprilysin/CD10 in ELISAs and Western blots. In sandwich immunoassays, less than 35% cross-reactivity with recombinant human (rh) Neprilysin is observed and less than 0.2% cross-reactivity with rhNeprilysin-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MME
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Mouse Neprilysin/CD10 Antibody (Catalog # AF1126)
ELISA Detection: Mouse Neprilysin/CD10 Biotinylated Antibody (Catalog # BAF1126)
Standard: Recombinant Mouse Neprilysin/CD10 (Catalog # 1126-ZN)
Publications
Read Publications using
AF1126 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neprilysin/CD10 Antibody [Unconjugated]

  • Atriopeptidase
  • CALLA
  • CALLAmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase)
  • CD10 antigen
  • CD10
  • CD10)
  • CD10membrane metallo-endopeptidase variant 1
  • Common acute lymphocytic leukemia antigen
  • DKFZp686O16152
  • EC 3.4.24
  • EC 3.4.24.11
  • Enkephalinase
  • EPN
  • Leu-19
  • membrane metallo-endopeptidase variant 2
  • membrane metallo-endopeptidase
  • MGC126681
  • MGC126707
  • MME
  • NEPmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA
  • Neprilysin
  • Neutral Endopeptidase 24.11
  • Neutral endopeptidase
  • NKH1
  • SFE
  • Skin fibroblast elastase

Background

Neprilysin (NEP, neutral endopeptidase 24.11, EC 3.4.24.11) is a zinc metallopeptidase expressed at the cell surface of a variety of cells. The enzyme functions both as an endopeptidase with a thermolysin-like specificity and as a dipeptidylcarboxypeptidase. NEP has been shown to be involved in the degradation of enkephalins in the mammalian brain and the inactivation of circulating atrial natriuretic peptide (1, 2). NEP has also been identified as the common acute lymphoblastic leukemia antigen (CALLA), and to be expressed on the surface of lymphocytes in some disease states (3). These and other observations have resulted in considerable clinical interest in NEP as a potential target for analgesics and antihypertensive drugs. NEP is also a major degrading enzyme of amyloid beta peptide (A beta ) in the brain, indicating that down-regulation of NEP activity, which could be caused by aging, can contribute to the development of Alzheimer’s disease by promoting A beta accumulation (4).

  1. Malfroy, B. et al. (1978) Nature 276:523.
  2. Kenny, A.J. and S.L. Stephenson (1988) FEBS Lett. 232:1.
  3. LeTarte, M. et al. (1988) J. Exp. Med. 168:1247.
  4. Itwata, N. et al. (2001) Science 292:1550.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neprilysin/CD10 Antibody (AF1126)(22)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 5 applications: ELISA Capture, IHC, IHC-Fr, IHC-P, Western Blot.


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ELISA Capture
(1)
IHC
(1)
IHC-Fr
(1)
IHC-P
(1)
Western Blot
(8)
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Mouse
(9)
Rat
(1)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using AF1126 Applications Species
Kim, N;Ju, IG;Jeon, SH;Lee, Y;Jung, MJ;Gee, MS;Cho, JS;Inn, KS;Garrett-Sinha, LA;Oh, MS;Lee, JK; Inhibition of microfold cells ameliorates early pathological phenotypes by modulating microglial functions in Alzheimer's disease mouse model Journal of neuroinflammation 2023-11-27 [PMID: 38012646] (Western Blot, Mouse) Western Blot Mouse
D Williams, BQ Yan, H Wang, L Negm, C Sackmann, C Verkuyl, V Rezai-Stev, S Eid, N Vediya, C Sato, JC Watts, H Wille, G Schmitt-Ul Somatostatin slows Abeta plaque deposition in aged APPNL-F/NL-F mice by blocking Abeta aggregation Scientific Reports, 2023-02-09;13(1):2337. 2023-02-09 [PMID: 36759538] (IHC, Western Blot, Mouse) IHC, Western Blot Mouse
K Kisler, AP Sagare, D Lazic, S Bazzi, E Lawson, CJ Hsu, Y Wang, A Ramanathan, AR Nelson, Z Zhao, BV Zlokovic Anti-malaria drug artesunate prevents development of amyloid-beta pathology in mice by upregulating PICALM at the blood-brain barrier Molecular Neurodegeneration, 2023-01-27;18(1):7. 2023-01-27 [PMID: 36707892] (Western Blot, Mouse) Western Blot Mouse
Fitzgerald G, Turiel G, Gorski T et al. MME(+) fibro-adipogenic progenitors are the dominant adipogenic population during fatty infiltration in human skeletal muscle Communications Biology 2023-01-27 [PMID: 36707617]
C Qu, QP Li, ZR Su, SP Ip, QJ Yuan, YL Xie, QQ Xu, W Yang, YF Huang, YF Xian, ZX Lin Nano-Honokiol ameliorates the cognitive deficits in TgCRND8 mice of Alzheimer's disease via inhibiting neuropathology and modulating gut microbiota Journal of advanced research, 2021-03-31;35(0):231-243. 2021-03-31 [PMID: 35024199] (Western Blot, Rat) Western Blot Rat
L Wang, Y Tang, AF Buckley, RF Spurney Blockade of the natriuretic peptide clearance receptor attenuates proteinuria in a mouse model of focal segmental glomerulosclerosis Physiological Reports, 2021-11-01;9(21):e15095. 2021-11-01 [PMID: 34755480] (Western Blot, Mouse) Western Blot Mouse
Laale F. Alawi, Sanjeev Dhakal, Sana E. Emberesh, Harshal Sawant, Anhar Hosawi, Unmesha Thanekar et al. Effects of Angiotensin II Type 1A Receptor on ACE2, Neprilysin and KIM-1 in Two Kidney One Clip (2K1C) Model of Renovascular Hypertension Frontiers in Pharmacology 2021-01-29 [PMID: 33708117]
A Thanabalas, AJ Chiang, C Morehouse, M Camara, S Hawkins, AE Keller, AC Koksal, CS Caceres, AA Berlin, N Holoweckyj, VN Takahashi, L Cheng, M de Los Rey, M Pelletier, AC Patera, B Sellman, S Hess, M Marelli, CC Boo, TS Cohen, A DiGiandome PD-L1+ neutrophils contribute to injury-induced infection susceptibility Science Advances, 2021-03-05;7(10):. 2021-03-05 [PMID: 33674305] (ELISA Capture, Mouse) ELISA Capture Mouse
SH Jeon, N Kim, YJ Ju, MS Gee, D Lee, JK Lee Phytohormone Abscisic Acid Improves Memory Impairment and Reduces Neuroinflammation in 5xFAD Mice by Upregulation of LanC-Like Protein 2 Int J Mol Sci, 2020-11-10;21(22):. 2020-11-10 [PMID: 33182586] (Western Blot, Mouse) Western Blot Mouse
Hong Ji, Aline M. A. de Souza, Bilkish Bajaj, Wei Zheng, Xie Wu, Robert C. Speth et al. Sex-Specific Modulation of Blood Pressure and the Renin-Angiotensin System by ACE (Angiotensin-Converting Enzyme) 2 Hypertension 2020-08-01 [PMID: 32564694]
Show All 22 Publications.

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Bioinformatics

Gene Symbol MME
Uniprot