CD53 Antibody (MEM-53) - BSA Free

Flow Cytometry: CD53 Antibody (MEM-53) [NB500-393] - Staining of CD53 in human peripheral blood cells with anti-CD53 (MEM-53) PE.

Flow Cytometry: CD53 Antibody (MEM-53) [NB500-393] - Staining of CD53 in human peripheral blood cells with anti-CD53 (MEM-53) Pacific BlueTM.

• Reactivity: Hu
• Applications: WB, Flow, IHC, IHC-Fr, IP, CyTOF-ready
• Clone: MEM-53
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

CD53 Antibody (MEM-53) - BSA Free Summary

• Immunogen: Leukocytes of patient suffering from a LGL-type leukemia.
• Specificity: The antibody MEM-53 reacts with CD53, a 32-40 kDa tetraspanin family glycoprotein exclusivelly expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells. The antibody MEM-53 reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F). HLDA IV; WS Code NL 59 HLDA V; WS Code B CD53.5 HLDA V; WS Code BP BP287 HLDA V; WS Code T T-096 HLDA V; WS Code X XB004
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD53
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:200
  • Immunoprecipitation 1:50
  • Western Blot 1:1000
• Application Notes: This antibody is suitable for discrimination of lymphomas from other tumors. Functional Application: The antibody MEM-53 induces activation of monocytes and B lymphocytes. This antibody is CyTOF ready.
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for CD53 Antibody (MEM-53) - BSA Free

• antigen MOX44 identified by monoclonal MRC-OX44
• CD53 antigentetraspanin-25
• CD53 glycoprotein
• CD53 molecule
• CD53 tetraspan antigen
• CD53
• cell surface antigen
• Cell surface glycoprotein CD53
• MOX44
• MOX44transmembrane glycoprotein
• Tetraspanin-25
• TSPAN25
• tspan-25
• TSPAN25leukocyte surface antigen CD53

Background

The antibody reacts with CD53 antigen, a glycoprotein of 32-40 kDa belonging to the tetraspans family (TM4SF). CD53 antigen is exclusivelly expressed on leucocytes (not present on platelets, red blood cells and non-hematopoietic cells). CD53 is important for signal transduction. CD53 cross-linking promotes activation of human B cells and rat macrophages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD53 Antibody (NB500-393)(1)

Publication using NB500-393

• Dunlock VME, Arp AB, Jansen E, Charrin S Dynamic regulation of CD45 by tetraspanin CD53 bioRxiv (FLOW, Human)

Bioinformatics

• Gene Symbol: CD53
• Entrez:
  • 963(Human)