CD53 Antibody (MEM-53)

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Flow Cytometry: CD53 Antibody (MEM-53) [NB500-393] - Staining of CD53 in human peripheral blood cells with anti-CD53 (MEM-53) PE.
Flow Cytometry: CD53 Antibody (MEM-53) [NB500-393] - Staining of CD53 in human peripheral blood cells with anti-CD53 (MEM-53) Pacific BlueTM.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, IHC-Fr, IP, CyTOF-ready
Clone
MEM-53
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

CD53 Antibody (MEM-53) Summary

Immunogen
Leukocytes of pacient suffering from a LGL-type leukemia.
Specificity
The antibody MEM-53 reacts with CD53, a 32-40 kDa tetraspanin family glycoprotein exclusivelly expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells. The antibody MEM-53 reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F). HLDA IV; WS Code NL 59 HLDA V; WS Code B CD53.5 HLDA V; WS Code BP BP287 HLDA V; WS Code T T-096 HLDA V; WS Code X XB004
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD53
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:200
  • Immunoprecipitation 1:50
  • CyTOF-ready
Application Notes
This antibody is suitable for discrimination of lymphomas from other tumors. Functional Application: The antibody MEM-53 induces activation of monocytes and B lymphocytes. This antibody is CyTOF ready.
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NB500-393 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for CD53 Antibody (MEM-53)

  • antigen MOX44 identified by monoclonal MRC-OX44
  • CD53 antigentetraspanin-25
  • CD53 glycoprotein
  • CD53 molecule
  • CD53 tetraspan antigen
  • CD53
  • cell surface antigen
  • Cell surface glycoprotein CD53
  • MOX44
  • MOX44transmembrane glycoprotein
  • Tetraspanin-25
  • TSPAN25
  • tspan-25
  • TSPAN25leukocyte surface antigen CD53

Background

The antibody reacts with CD53 antigen, a glycoprotein of 32-40 kDa belonging to the tetraspans family (TM4SF). CD53 antigen is exclusivelly expressed on leucocytes (not present on platelets, red blood cells and non-hematopoietic cells). CD53 is important for signal transduction. CD53 cross-linking promotes activation of human B cells and rat macrophages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD53 Antibody (NB500-393)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow.


Filter By Application
Flow
(1)
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(1)
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Showing Publication 1 - 1 of 1.
Publication using NB500-393 Applications Species
Dunlock VME, Arp AB, Jansen E, Charrin S Dynamic regulation of CD45 by tetraspanin CD53 bioRxiv (Flow, Human) Flow Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CD53 Antibody (NB500-393) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB500-393AF350
Alexa Fluor 405 NB500-393AF405
Alexa Fluor 488 NB500-393AF488
Alexa Fluor 532 NB500-393AF532
Alexa Fluor 594 NB500-393AF594
Alexa Fluor 647 NB500-393AF647
Alexa Fluor 700 NB500-393AF700
Alexa Fluor 750 NB500-393AF750
Allophycocyanin NB500-393APC
Allophycocyanin/Cy7 NB500-393APCCY7
Biotin NB500-393B
DyLight 350 NB500-393UV
DyLight 405 NB500-393V
DyLight 488 NB500-393G
DyLight 550 NB500-393R
DyLight 594 NB500-393DL594
DyLight 650 NB500-393C
DyLight 680 NB500-393FR
DyLight 755 NB500-393IR
FITC NB500-393F
HRP NB500-393H
Janelia Fluor 549 NB500-393JF549
Janelia Fluor 646 NB500-393JF646
PE NB500-393PE
PE/Atto594 NB500-393PEATT594
PE/Cy5.5 NB500-393PECY55
PE/Cy7 NB500-393PECY7
PerCP NB500-393PCP

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Target of the antiproliferative 1 (TAPA1), also known as CD81, is found in the plasma membrane in lymphocytes and plays an important role in the regulation of lymphoma cell growth. This transmembrane 4 superfamily (TM4SF) protein is primarily found on...  Read full blog post.

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