CD9 Antibody (MEM-61) - BSA Free

Images

 
Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Staining of human peripheral blood with anti-CD9 (MEM-61) Alexa Fluor® 647.
Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Surface staining of NALM-6 human pre-B cell leukemia cell line Total viable cells were used for analysis.
Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD9 (MEM-61) Dy161.
Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Surface staining of human peripheral blood with anti-CD9 (MEM-61) biotin / streptavidin-APC.
Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Surface staining of human peripheral blood with anti-CD9 (MEM-61) APC-CyTM7.
Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Surface staining of human peripheral blood with anti-CD9 (MEM-61) Pacific BlueTM.
Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Staining of human peripheral blood with anti-CD9 (MEM-61) APC.
Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Staining of human peripheral blood with anti-CD9 (MEM-61) PE.
CyTOF-ready: CD9 Antibody (MEM-61) [NB500-327] - Analysis of CD9 antibody on human tissue. Scale, 50um. Image from verified customer review.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, Func, IHC, CyTOF-ready
Clone
MEM-61
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

CD9 Antibody (MEM-61) - BSA Free Summary

Additional Information
Clone MEM-61 was used by HLDA to establish CD designation.
Immunogen
Pre-B cell line NALM-6
Specificity
The antibody MEM-61 recognizes an epitope on second extracellular domain (EC2) of CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes. HLDA VI; WS Code P P-15
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD9
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA
  • Flow Cytometry 1-4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 20 ug/ml
  • In vitro assay
  • Western Blot 2-4 ug/ml
Application Notes
Western Blot -The antibody MEM-61 induces FcR-dependent platelet aggregation. This antibody is CyTOF ready.Use in In vitro reported in scientific literature (PMID: 28823976). Use in ELISA reported in secitific publication PMID: 32393780. Clone MEM-61 has been used for extracellular vesicle flow cytometry.
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
CD9 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB500-327 in the following applications:

Publications
Read Publications using
NB500-327 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for CD9 Antibody (MEM-61) - BSA Free

  • BA2
  • BA-2/p24 antigen
  • BTCC-1
  • CD9 antigen
  • CD9 molecule
  • CD9
  • Cell growth-inhibiting gene 2 protein
  • DRAP-27
  • Leukocyte antigen MIC3
  • MIC3
  • motility related protein-1,5H9 antigen
  • Motility-related protein
  • MRP-1FLJ99568
  • p24
  • tetraspanin-29
  • TSPAN29
  • TSPAN-29
  • TSPAN29MIC3CD9 antigen (p24)

Background

CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD9 Antibody (NB500-327)(16)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 6 applications: CyTOF-ready, ELISA, FLOW, ICC/IF, In vitro, WB.


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CyTOF-ready
(1)
ELISA
(1)
FLOW
(3)
ICC/IF
(1)
In vitro
(1)
WB
(1)
All Applications
Filter By Species
Human
(8)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB500-327 Applications Species
Koel M, KrjutSkov K, Saare M Et al. Human endometrial cell-type-specific RNA sequencing provides new insights into the embryo-endometrium interplay Hum Reprod Open 2022-11-07 [PMID: 36339249] (FLOW, Human)

Details:
Citation using the FITC version of this antibody.
FLOW Human
Radnaa E, Richardson L, Goldman B Et al. Stress signaler p38 mitogen-activated kinase activation: a cause for concern? Clin Sci (Lond) 2022-10-17 [PMID: 36250628] (CyTOF-ready, Human)

Details:
Citation using the Azide and BSA Free version of this antibody.
CyTOF-ready Human
Irmscher S, Zipfel SLH, Halder LD Et al. Factor H-related protein 1 (FHR-1) is associated with atherosclerotic cardiovascular disease Scientific reports 2021-11-18 [PMID: 34795372]
Sahu SS, Cavallaro S, HAAg P et al. Exploiting Electrostatic Interaction for Highly Sensitive Detection of Tumor-Derived Extracellular Vesicles by an Electrokinetic Sensor ACS Appl Mater Interfaces 2021-09-15 [PMID: 34473477]

Details:
Citation using the Biotin format of this antibody.
Han C, Kang H, Yi J et al. Single-vesicle imaging and co-localization analysis for tetraspanin profiling of individual extracellular vesicles Extracell Vesicles 2021-01-10 [PMID: 33456726]
Zhou J, Wu Z, Hu J et al High-throughput single-EV liquid biopsy: Rapid, simultaneous, and multiplexed detection of nucleic acids, proteins, and their combinations Sci Adv 2020-11-21 [PMID: 33219024] (Human)

Details:
Citation using the Alexa Fluor 488 version of this antibody.
Human
Han C, Kang M, Kang H et al. Characterization of extracellular vesicle and virus-like particles by single vesicle tetraspanin analysis Sensors and Actuators B: Chemical 2023-05-01 [PMID: 30236130] (ICC/IF, Human) ICC/IF Human
Sun D, Hu TY. A low cost mobile phone dark-field microscope for nanoparticle-based quantitative studies. Biosens Bioelectron. 2018-01-15 [PMID: 28823976] (In vitro)

Details:
This citation used the Biotin form of this antibody.
In vitro
Krjutskov K, Katayama S, Saare M et al. Single-cell transcriptome analysis of endometrial tissue. Hum. Reprod. 2016-02-13 [PMID: 26874359] (FLOW, Human)

Details:
This citation used the FITC version of this antibody.
FLOW Human
Saare M, Rekker K, Laisk-Podar T et al. High-Throughput Sequencing Approach Uncovers the miRnome of Peritoneal Endometriotic Lesions and Adjacent Healthy Tissues PLoS OnE 2014-11-12 [PMID: 25386850] (FLOW, Human)

Details:
This citation used the FITC version of this antibody.
FLOW Human
Show All 16 Publications.

Review for CD9 Antibody (NB500-327) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB500-327:
Filter by Applications
CyTOF
(1)
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
CyTOF CD9 NB500-327
Enlarge
4
reviewed by:
Enkhtuya Radnaa
CyTOF Human 01/04/2022
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Summary

ApplicationCyTOF
Sample TestedHuman tissue origin
SpeciesHuman
Lot534847

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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1
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Enkhtuya Radnaa
01/04/2022
Application: CyTOF
Species: Human

Bioinformatics

Gene Symbol CD9