CD9 Antibody (MEM-61)

Images

 
Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Staining of human peripheral blood with anti-CD9 (MEM-61) Alexa Fluor® 647.
Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Surface staining of NALM-6 human pre-B cell leukemia cell line Total viable cells were used for analysis.
Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD9 (MEM-61) Dy161.
Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Surface staining of human peripheral blood with anti-CD9 (MEM-61) biotin / streptavidin-APC.
Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Surface staining of human peripheral blood with anti-CD9 (MEM-61) APC-CyTM7.
Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Surface staining of human peripheral blood with anti-CD9 (MEM-61) Pacific BlueTM.
Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Staining of human peripheral blood with anti-CD9 (MEM-61) APC.
Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Staining of human peripheral blood with anti-CD9 (MEM-61) PE.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-P, In vitro, CyTOF-ready
Clone
MEM-61
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

CD9 Antibody (MEM-61) Summary

Immunogen
Pre-B cell line NALM-6
Specificity
The antibody MEM-61 recognizes an epitope on second extracellular domain (EC2) of CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes. HLDA VI; WS Code P P-15
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD9
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2-4 ug/ml
  • ELISA
  • Flow Cytometry 5 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 20 ug/ml
  • In vitro assay
  • CyTOF-ready
Application Notes
Western Blot -The antibody MEM-61 induces FcR-dependent platelet aggregation. This antibody is CyTOF ready.Use in In vitro reported in scientific literature (PMID: 28823976). Use in ELISA reported in secitific publication PMID: 32393780
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
CD9 Overexpression Lysate
CD9 Knockout HeLa Cell Lysate
Publications
Read Publications using
NB500-327 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for CD9 Antibody (MEM-61)

  • BA2
  • BA-2/p24 antigen
  • BTCC-1
  • CD9 antigen
  • CD9 molecule
  • CD9
  • Cell growth-inhibiting gene 2 protein
  • DRAP-27
  • Leukocyte antigen MIC3
  • MIC3
  • motility related protein-1,5H9 antigen
  • Motility-related protein
  • MRP-1FLJ99568
  • p24
  • tetraspanin-29
  • TSPAN29
  • TSPAN-29
  • TSPAN29MIC3CD9 antigen (p24)

Background

CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD9 Antibody (NB500-327)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ELISA, Flow, In vitro, WB.


Filter By Application
ELISA
(1)
Flow
(2)
In vitro
(1)
WB
(1)
All Applications
Filter By Species
Human
(4)
All Species
Showing Publications 1 - 9 of 9.
Publications using NB500-327 Applications Species
Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018
Halder LD, Jo EAH, Hasan MZ et al. Immune modulation by complement receptor 3-dependent human monocyte TGF-beta 1-transporting vesicles Nat Commun May 11 2020 [PMID: 32393780] (ELISA, Human) ELISA Human
Sun D, Hu TY. A low cost mobile phone dark-field microscope for nanoparticle-based quantitative studies. Biosens Bioelectron. 2018 Jan 15 [PMID: 28823976] (In vitro)

Details:
This citation used the Biotin form of this antibody.
In vitro
Krjutskov K, Katayama S, Saare M et al. Single-cell transcriptome analysis of endometrial tissue. Hum. Reprod. 2016 Feb 13 [PMID: 26874359] (Flow, Human)

Details:
This citation used the FITC version of this antibody.
Flow Human
Saare M, Rekker K, Laisk-Podar T et al. High-Throughput Sequencing Approach Uncovers the miRnome of Peritoneal Endometriotic Lesions and Adjacent Healthy Tissues PLoS OnE 2014 Nov 12 [PMID: 25386850] (Flow, Human)

Details:
This citation used the FITC version of this antibody.
Flow Human
Bronisz A, Wang Y, Nowicki MO et al. Extracellular vesicles modulate the glioblastoma microenvironment via a tumor suppression signaling network directed by miR-1. Cancer Res. 2/1/2014 [PMID: 24310399] (WB, Human) WB Human
Lafleur MA, Xu D, Hemler ME. Tetraspanin proteins regulate membrane type-1 matrix metalloproteinase-dependent pericellular proteolysis Mol Biol Cell 2009 Apr [PMID: 19211836]

Details:
This citation used the Allophycocyanin version of this antibody.
Singh AB, Sugimoto K, Dhawan P, Harris RC. : Juxtacrine activation of EGFR regulates claudin expression and increases transepithelial resistance Am J Physiol Cell Physiol 2007 Nov [PMID: 17855771]

Details:
This citation used the Allophycocyanin version of this antibody.
Stockl J, Majdic O, Fischer G et al. Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA-A1-restricted, hapten-specific CTL J Immunol [PMID: 11509616]

Details:
Used the Allophycocyanin form of this antibody.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CD9 Antibody (NB500-327) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB500-327AF350
Alexa Fluor 405 NB500-327AF405
Alexa Fluor 488 NB500-327AF488
Alexa Fluor 532 NB500-327AF532
Alexa Fluor 594 NB500-327AF594
Alexa Fluor 647 NB500-327AF647
Alexa Fluor 700 NB500-327AF700
Alexa Fluor 750 NB500-327AF750
Allophycocyanin NB500-327APC
Allophycocyanin/Cy7 NB500-327APCCY7
Biotin NB500-327B
DyLight 350 NB500-327UV
DyLight 405 NB500-327V
DyLight 488 NB500-327G
DyLight 550 NB500-327R
DyLight 594 NB500-327DL594
DyLight 650 NB500-327C
DyLight 680 NB500-327FR
DyLight 755 NB500-327IR
FITC NB500-327F
HRP NB500-327H
Janelia Fluor 549 NB500-327JF549
Janelia Fluor 646 NB500-327JF646
PE NB500-327PE
PE/Atto594 NB500-327PEATT594
PE/Cy5.5 NB500-327PECY55
PE/Cy7 NB500-327PECY7
PerCP NB500-327PCP

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Bioinformatics

Gene Symbol CD9