CD63 Antibody (H5C6) - BSA Free

Western Blot: CD63 Antibody (H5C6) [NBP2-42225] - THP1 whole cell protein was separated by SDS-PAGE on a 12% gel and transferred to PVDF membrane. The membrane was probed with anti-CD63 antibody at 2 ug/mL and detected with an anti-mouse HRP secondary antibody using chemiluminescence.

Immunocytochemistry/Immunofluorescence: CD63 Antibody (H5C6) [NBP2-42225] - The CD63 (H5C6) antibody was tested in HeLa cells at a 1:50 dilution against DyLight 488 (Green). Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.

Immunocytochemistry/Immunofluorescence: CD63 Antibody (H5C6) [NBP2-42225] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-CD63 Antibody [H5C6] conjugated to DyLight 550 (NBP2-42225R) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Flow Cytometry: CD63 Antibody (H5C6) [NBP2-42225] - An intracellular stain was performed on SK-MEL-28 cells with CD63 (H5C6) Antibody NBP2-42225R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.

Immunocytochemistry/Immunofluorescence: CD63 Antibody (H5C6) [NBP2-42225] - MDCK cells stained with CD63 antibody at a dilution of 1:50 followed by Donkey anti-mouse secondary antibody conjugated with Alexa Fluor 488 (1:500). Nuclei were stained with Hoechst 33342. ICC/IF image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: CD63 Antibody (H5C6) [NBP2-42225] - U2OS cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-CD63 Antibody [H5C6] conjugated to Alexa Fluor 488 (NBP2-42225AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/Immunofluorescence: CD63 Antibody (H5C6) [NBP2-42225] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-CD63 Antibody [H5C6] conjugated to Biotin (NBP2-42225B) at 5 ug/ml for 60 minutes at room temperature and detected with Streptavidin Protein conjugated to DyLight 488 at a 1:500 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/Immunofluorescence: CD63 Antibody (H5C6) [NBP2-42225] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-CD63 Antibody [H5C6] conjugated to Biotin (NBP2-42225B) at 5 ug/ml for 1 hour at room temperature and detected with Streptavidin conjugated to DyLight 488. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/Immunofluorescence: CD63 Antibody (H5C6) [NBP2-42225] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-CD63 [H5C6] conjugated to Alexa Fluor 488 [NBP2-42225AF488] at 10ug/mL for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Western Blot: CD63 Antibody (H5C6) - BSA Free [NBP2-42225] - Analysis of common EV markers, where 10 ug of total protein was loaded in each lane. cASCs-EVs expressed CD63 and CD9, while beta actin and lamin A showed lower expression. The displayed data represent at least three repeated experiments with consistent results. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32040478/) licensed under a CC-BY licence.

Flow Cytometry: CD63 Antibody (H5C6) [NBP2-42225] - An intracellular stain was performed on HeLa cells with CD63 [H5C6] Antibody NBP2-42225AF594 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 594.

Western Blot: CD63 Antibody (H5C6) [NBP2-42225] - The same samples and volumes were run under reducing and non-reducing conditions. All procedures were performed in parallel for both conditions. L- ladder +- exosomes. Image visualized with HRP linked to secondary antibody. Western blot image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: CD63 Antibody (H5C6) [NBP2-42225] - HEK cells stained with CD63 antibody at a dilution of 1:50 followed by Donkey anti-mouse secondary antibody conjugated with Alexa Fluor 488 (1:500). Nuclei were stained with Hoechst 33342. ICC/IF image submitted by a verified customer review.

Flow Cytometry: CD63 Antibody (H5C6) [NBP2-42225] - Human peripheral blood cells were stained (2 x 10^6 cells/mL) using the anti-CD63 antibody (Blue) at a dilution of 1:1000. Signal was detected using a Gt x Ms DyLight 488 Secondary and gated to the monocyte/granulocyte cell populations. Isotype was Mouse IgG1 kappa (orange). Data collected on BD FACS Calibur flow cytometer.

Flow Cytometry: CD63 Antibody (H5C6) [NBP2-42225] - A431 cells were stained (1 x10^6 cells/mL) using the anti-CD63 antibody at a 1:1000 dilution (blue). Signal was detected with Gt x Ms DyLight 488 secondary. Isotype control (orange).

Flow (Intracellular): CD63 Antibody (H5C6) [NBP2-42225] - An intracellular stain was performed on HepG2 cells with CD63 Antibody (H5C6) NBP2-42225AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Flow Cytometry: CD63 Antibody (H5C6) [NBP2-42225] - An intracellular stain was performed on HeLa cells with CD63 Antibody (H5C6) NBP2-42225APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.

Flow Cytometry: CD63 Antibody (H5C6) [NBP2-42225] - An intracellular stain was performed on SK-MEL-28 cells with CD63 Antibody (H5C6) NBP2-42225AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Flow Cytometry: CD63 Antibody (H5C6) [NBP2-42225] - An intracellular stain was performed on HeLa cells with CD63 [H5C6] Antibody NBP2-42225AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

• Reactivity: Hu, Ca
• Applications: WB, DB, ELISA, EM, Flow, Flow-IC, Func, ICC/IF, In vitro, IP, B/N, IHC-WhMt
• Clone: H5C6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

CD63 Antibody (H5C6) - BSA Free Summary

• Additional Information: Clone H5C6 was used by HLDA to establish CD designation.
• Immunogen: Human splenic adherent cells.
• Marker: Exosome Marker
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD63
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Dot Blot
  • Electron Microscopy
  • ELISA
  • Flow (Intracellular)
  • Flow Cytometry 1:1000
  • Functional
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunohistochemistry Whole-Mount
  • Immunoprecipitation
  • In vitro assay
  • Western Blot
• Application Notes: Use in B/N reported in scientific literature (PMID:35602933) Use in Electron Microscopy reported in scientific literature (PMID: 16735575). Use in functional reported in scientific literature (PMID: 9811687). Use in In vitro and Immunohistochemistry-Whole mount reported in scientific literature (PMID: 21464080)..

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for CD63 Antibody (H5C6) - BSA Free

• CD_antigen: CD63
• CD63 antigen (melanoma 1 antigen)
• CD63 antigen
• CD63 molecule
• CD63
• Granulophysin
• Lamp-3
• Lysosomal-associated membrane protein 3
• lysosome-associated membrane glycoprotein 3
• ME491
• melanoma 1 antigen
• Melanoma-associated antigen ME491
• melanoma-associated antigen MLA1
• MLA1
• Ocular melanoma-associated antigen
• OMA81H
• tetraspanin-30
• Tspan30
• tspan-30

Background

CD63 (cluster of differentiation 63), also known as lysosome associated membrane protein 3 (LAMP-3), is a membrane spanning glycoprotein with a molecular mass ranging from 30-60 kDa that was the first characterized member of the tetraspanin superfamily (1,2). CD63 is ubiquitously expressed and largely present on the cell surface in the endosomal system (1-3). More specifically, it is generally present in multivesicular bodies (MVBs), also called late endosomes, and lysosomes (1). The CD63 molecule is a total of 238 amino acids (aa), has four hydrophobic membrane-spanning regions and three N-glycosylation sites, and the encoded protein has a theoretical molecular weight of 25 kDa (2,3). CD63 both directly and indirectly interacts with other proteins including integrins, cell surface receptors, other tetraspanins, kinases, and adapter proteins, to name a few (1). CD63 is involved in many cell processes including cell survival and activation, cell adhesion, invasion, and migration (1). Additionally, CD63 has been shown to interact with tissue inhibitor of metalloproteinase 1 (TIMP-1), which originally thought to be an inhibitor of cancer progression has recently been shown to have cancer promoting properties as well (1,4). The CD63-TIMP-1 interaction has been shown to activate the PI3K/AKT pathway in lung adenocarcinoma cells and also promote survival and invasion of acute myeloid leukemia cells (1).CD63 is a useful flow cytometry marker for basophil granulocytes and can be used for the basophil activation test (BAT) to assess IgE-mediated allergy response (5). As CD63 was initially discovered on activated blood platelets and is a lysosomal-associated protein it makes sense it would be involved in platelet or lysosomal-related disorders (1,2). More precisely, CD63 is associated with Hermansky-Pudlak syndrome, a disease characterized by albinism and platelet storage pool deficiency (6).

References

1. Pols, M. S., & Klumperman, J. (2009). Trafficking and function of the tetraspanin CD63. Experimental cell research. https://doi.org/10.1016/j.yexcr.2008.09.020

2. Metzelaar, M. J., Wijngaard, P. L., Peters, P. J., Sixma, J. J., Nieuwenhuis, H. K., & Clevers, H. C. (1991). CD63 antigen. A novel lysosomal membrane glycoprotein, cloned by a screening procedure for intracellular antigens in eukaryotic cells. The Journal of biological chemistry.

3. Horejsi, V., & Vlcek, C. (1991). Novel structurally distinct family of leucocyte surface glycoproteins including CD9, CD37, CD53 and CD63. FEBS letters. https://doi.org/10.1016/0014-5793(91)80988-f

4. Eckfeld, C., HauBler, D., Schoeps, B., Hermann, C. D., & Kruger, A. (2019). Functional disparities within the TIMP family in cancer: hints from molecular divergence. Cancer metastasis reviews. https://doi.org/10.1007/s10555-019-09812-6

5. Hoffmann, H. J., Santos, A. F., Mayorga, C., Nopp, A., Eberlein, B., Ferrer, M., Rouzaire, P., Ebo, D. G., Sabato, V., Sanz, M. L., Pecaric-Petkovic, T., Patil, S. U., Hausmann, O. V., Shreffler, W. G., Korosec, P., & Knol, E. F. (2015). The clinical utility of basophil activation testing in diagnosis and monitoring of allergic disease. Allergy. https://doi.org/10.1111/all.12698

6. Dell'Angelica, E. C., Shotelersuk, V., Aguilar, R. C., Gahl, W. A., & Bonifacino, J. S. (1999). Altered trafficking of lysosomal proteins in Hermansky-Pudlak syndrome due to mutations in the beta 3A subunit of the AP-3 adaptor. Molecular cell. https://doi.org/10.1016/s1097-2765(00)80170-7

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD63 Antibody (NBP2-42225)(43)

Publications using NBP2-42225

• Kim S, Lee K, Choi YS et al. Mitochondrial double-stranded RNAs govern the stress response in chondrocytes to promote osteoarthritis development Cell reports 2022-08-09 [PMID: 35947956] (WB)
  
  Details:
  Supplementary Figure S5.
• Hsu CH, Ma HP, Ong JR et al. Cancer-Associated Exosomal CBFB Facilitates the Aggressive Phenotype, Evasion of Oxidative Stress, and Preferential Predisposition to Bone Prometastatic Factor of Breast Cancer Progression Disease markers 2022-07-19 [PMID: 35903297]
• Conkright WR, Beckner ME, Sterczala AJ et al. Resistance Exercise Differentially Alters Extracellular Vesicle Size and Subpopulation Characteristics in Healthy Men and Women: An Observational Cohort Study Physiological genomics 2022-07-11 [PMID: 35816651] (FLOW, Human)
• Lee C, Chen Y, Hsiao A Et al. Demystifying the long noncoding RNA landscape of small EVs derived from human mesenchymal stromal cells J Adv Res 2022-07-01 [PMID: 35777918]
• Fleming Martinez A, DOppler H, Bastea L et al. Ym1+ macrophages orchestrate fibrosis, lesion growth and progression during development of murine pancreatic cancer iScience 2022-04-01 [PMID: 35602933] (B/N, Mouse)
• Dou X, Hua Y, Chen Z et al. Extracellular Vesicles Containing PD-L1 Contribute to CD8 + T-Cell Immune Suppression and Predict Poor Outcomes in Small Cell Lung Cancer Clin Exp Immunol 2022-05-13 [PMID: 35553630]
• Wright AB The isolation, culture-expansion, cryopreservation, characterization, and properties of umbilical cord-derived mesenchymal stromal cells and their extracellular vesicles Thesis 2021-01-01
• Lin W, Yuan L, Gao Z et al. An Integrated Sample-to-Answer SERS Platform for Multiplex Phenotyping of Extracellular Vesicles SSRN Electronic Journal 2023-01-28
• Beckner ME, Conkright WR, Sahu A Et al. Utility of extracellular vesicles as a potential biological indicator of physiological resilience during military operational stress Physiol Rep 2022-04-04 [PMID: 35373929] (ICC/IF, Human)
  
  Details:
  Citation using the Alexa Fluor 700 version of this antibody.
• Kolka CM, Webster J, Lepletier A et al. C5b-9 Membrane Attack Complex Formation and Extracellular Vesicle Shedding in Barrett's Esophagus and Esophageal Adenocarcinoma Frontiers in immunology 2022-03-08 [PMID: 35345676] (IA, Human)
• Conkright WR, Beckner ME, Sahu A et al. Men and Women Display Distinct Extracellular Vesicle Biomarker Signatures in Response to Military Operational Stress Journal of applied physiology (Bethesda, Md. : 1985) 2022-03-17 [PMID: 35297690]
• Rudy MJ, Coughlan C, Hixon AM et al. Density Analysis of Enterovirus D68 Shows Viral Particles Can Associate with Exosomes Microbiology spectrum 2022-02-23 [PMID: 35170992] (IP, Human)
• Li W, Xu J, Cheng L et al. RelB promotes the migration and invasion of prostate cancer DU145 cells via exosomal ICAM1 in vitro Cellular signalling 2021-12-18 [PMID: 34933092] (WB, Human)
• Leroux E, Perbet R, Caillierez R Et al. Extracellular vesicles: Major actors of heterogeneity in tau spreading among human tauopathies Molecular therapy : the journal of the American Society of Gene Therapy 2021-09-24 [PMID: 34563677] (EM, Human)
• Bellio MA, Young KC, Milberg J et al. Amniotic fluid-derived extracellular vesicles: characterization and therapeutic efficacy in an experimental model of bronchopulmonary dysplasia Cytotherapy 2021-09-17 [PMID: 34538718] (Human, ICC/IF)
  
  Details:
  Citation using the DyLight 550 format of this antibody.
• Lee J, Lee SA, Gu NY et al. Canine Natural Killer Cell-Derived Exosomes Exhibit Antitumor Activity in a Mouse Model of Canine Mammary Tumor BioMed research international 2021-09-04 [PMID: 34527741] (WB, Canine)
• Teshima T, Yuchi Y, Suzuki R Et al. Immunomodulatory Effects of Canine Adipose Tissue Mesenchymal Stem Cell-Derived Extracellular Vesicles on Stimulated CD4+ T Cells Isolated from Peripheral Blood Mononuclear Cells Journal of immunology research 2021-08-14 [PMID: 34447855]
• Wang J, Wuethrich A, Lobb RJ et al. Characterizing the Heterogeneity of Small Extracellular Vesicle Populations in Multiple Cancer Types via an Ultrasensitive Chip ACS sensors 2021-07-15 [PMID: 34264628]
• Pederson PJ, Liang H, Filonov D, Mooberry SL. Eribulin and Paclitaxel Differentially Alter Extracellular Vesicles and Their Cargo from Triple-Negative Breast Cancer Cells Cancers (Basel) 2021-07-02 [PMID: 34205051]
  
  Details:
  This citation used the Alexa Fluor 488 format of this antibody.
• Krishnamachary B, Mahajan A, Kumar A et al. Extracellular vesicle TGF-beta 1 is linked to cardiopulmonary dysfunction in HIV American journal of respiratory cell and molecular biology 2021-05-20 [PMID: 34014809]
• Arab T, Mallick ER, Huang Y et al. Characterization of extracellular vesicles and synthetic nanoparticles with four orthogonal single-particle analysis platforms Journal of extracellular vesicles 2021-04-01 [PMID: 33850608]
• Tomishige N, Murate M, Didier P et al. The use of pore-forming toxins to image lipids and lipid domains Methods in Enzymology 2021-02-26 [PMID: 33712198]
• Zhou J, Wu Z, Hu J et al High-throughput single-EV liquid biopsy: Rapid, simultaneous, and multiplexed detection of nucleic acids, proteins, and their combinations Sci Adv 2020-11-21 [PMID: 33219024] (ICC/IF, Human)
  
  Details:
  Citation using the Alexa Fluor 488 version of this antibody.
• Wang J, Wuethrich A, Sina AA et al. Tracking extracellular vesicle phenotypic changes enables treatment monitoring in melanoma Sci Adv 2020-02-01 [PMID: 32133394] (WB, Human)
• Kumar SR, Kimchi ET, Manjunath Y et al. RNA cargos in extracellular vesicles derived from blood serum in pancreas associated conditions Sci Rep 2020-02-18 [PMID: 32071328] (WB, Human)
• An J H, Li Q et al. TSG-6 in extracellular vesicles from canine mesenchymal stem/stromal is a major factor in relieving DSS-induced colitis. PLoS One 2020-11-02 [PMID: 32040478] (WB, Canine)
• Zeng X, Carlin CR Adenovirus early region 3 RID alpha protein limits NF kappa B signaling through stress-activated EGF receptors PLoS Pathog. 2019-08-01 [PMID: 31425554]
• Cui, X, Liu, Y Et al. Circulating Exosomes Activate Dendritic Cells and Induce Unbalanced CD4+ T Cell Differentiation in Hashimoto Thyroiditis. J Clin Endocrinol Metab 2019-10-01 [PMID: 31199456] (FLOW, Rat)
• Kuo HF, Hsieh CC, Wang SC et al. Simvastatin Attenuates Cardiac Fibrosis via Regulation of Cardiomyocyte-Derived Exosome Secretion J Clin Med 2019-06-04 [PMID: 31167519] (WB, Human)
• Huang WT, Chong IW, Chen HL et al. Pigment epithelium-derived factor inhibits lung cancer migration and invasion by upregulating exosomal thrombospondin 1. Cancer Lett. 2018-11-12 [PMID: 30439539] (WB, Human)
• Rafat N Theoretical and Experimental Studies of Multistep Electrochemical Biosensors ACS Nano 2018-02-24 [PMID: 29474060]
• Bilawchuk LM, Griffiths CD, Jensen LD et al. The Susceptibilities of Respiratory Syncytial Virus to Nucleolin Receptor Blocking and Antibody Neutralization are Dependent upon the Method of Virus Purification Viruses 2017-08-03 [PMID: 28771197] (WB, Human)
• Green LR, Monk PN, Partridge LJ, Morris Pet al. Cooperative role for tetraspanins in adhesin-mediated attachment of bacterial species to human epithelial cells.. Infect Immun. 2011-06-01 [PMID: 21464080] (ICC/IF, In vitro, Human)
• Parthasarathy V, Martin F, Higginbottom A et al. Distinct roles for tetraspanins CD9, CD63 and CD81 in the formation of multinucleated giant cells.. Immunology. 2009-06-01 [PMID: 19489128] (Func, In vitro, FLOW, WB, Human)
  
  Details:
  CD63 antibody (clone H5C6) was used for FLOW application on human peripheral blood monocytes which were isolated from peripheral whole blood of healthy volunteers and the assay implicated - culture of adherent monocytes in the presence or absence of 10 ug/ml of Con A lectin, harvesting followed by washing with B/B/N buffer ( balanced salt solution/BSS with 0·2% BSA and 0·1% azide), incubation of 2 × 10^5 cells with 10ug of primary antibody or IgG1 isotype control on ice for 45 minutes, 2X washing with B/B/N buffer followed by 30 minutes incubation with FITC-labelled anti-mouse immunoglobulin , washing and analysis on flow cytometer (data not shown). The antibody was also used in functional/in-vitro assay for analysing the effects of anti-tetraspanin immunoglobulins on concanavalin A (Con A) induced monocyte fusion / multinucleated giant cells formation (Figure 2 and Figure 3), and for WB analysis of tetraspanin recombinant protein- extracellular domain-GST (Figure S1).
• Tang Y, Leao IC, Coleman EM et al. Deficiency of niemann-pick type C-1 protein impairs release of human immunodeficiency virus type 1 and results in Gag accumulation in late endosomal/lysosomal compartments. J Virol 2009-08-01 [PMID: 19474101] (ICC/IF, Human)
• Nydegger S, Khurana S, Krementsov DN et al. Mapping of tetraspanin-enriched microdomains that can function as gateways for HIV-1.. J Cell Biol. 2006-06-05 [PMID: 16735575] (ICC/IF, EM, Human)
• Nguyen DG, Booth A, Gould SJ, Hildreth JE. Evidence that HIV budding in primary macrophages occurs through the exosome release pathway.. J Biol Chem. 2003-12-26 [PMID: 14561735] (FLOW, ELISA, Cytometric Bead Assay Standard, Human)
• Klein-Soyer C, Azorsa DO, Cazenave JP, Lanza F. CD9 participates in endothelial cell migration during in vitro wound repair.. Arterioscler Thromb Vasc Biol. 2000-02-01 [PMID: 10669631] (Func, FLOW, ICC/IF, In vitro, Human)
• Azorsa DO, Moog S, Cazenave JP, Lanza F. A general approach to the generation of monoclonal antibodies against members of the tetraspanin superfamily using recombinant GST fusion proteins.. J Immunol Methods. 1999-10-29 [PMID: 10556689] (ELISA, WB)
• Hildreth JE. Syncytium-inhibiting monoclonal antibodies produced against human T-cell lymphotropic virus type 1-infected cells recognize class II major histocompatibility complex molecules and block by protein crowding.. J Virol. 1998-12-01 [PMID: 9811687] (Func, Human)
• Azorsa DO, Hyman JA, Hildreth JE. CD63/Pltgp40: a platelet activation antigen identical to the stage-specific, melanoma-associated antigen ME491.. Blood. 1991-07-15 [PMID: 2070066] (IP, Human)
• Hildreth JE, Derr D, Azorsa DO et al. Characterization of a novel self-associating Mr 40,000 platelet glycoprotein.. Blood. 1991-01-01 [PMID: 1984792] (IP, WB, FLOW, Human)

Reviews for CD63 Antibody (NBP2-42225) (6)

Average Rating: 3.7

(Based on 6 reviews)

We have 6 reviews tested in 2 species: Human, Canine.

reviewed by: Huxley Hoffman ICC Canine 09/01/2022

Summary

• Application: Immunocytochemistry
• Sample Tested: MDCK canine kidney epithelial cell line
• Species: Canine
• Lot: C-9

reviewed by: Adrienne Wright DB Canine 10/09/2020

Summary

• Application: Dot Blot
• Sample Tested: cell lysate canine umbilical cord-derived mesenchymal stromal cells
• Species: Canine
• Lot: C-2

reviewed by: Nora Mellouk WB Human 10/02/2019

Summary

• Application: Western Blot
• Sample Tested: Hela whole cell lysate
• Species: Human
• Lot: B-4

reviewed by: Magdalena Grunt WB Human 09/18/2017

Summary

• Application: Western Blot
• Sample Tested: Blood plasma,Urine
• Species: Human
• Lot: A-1

Comments

• Comments: Antibody labelled with HRP

reviewed by: Verified Customer ICC/IF Human 11/01/2016

Summary

• Application: Immunocytochemistry/Immunofluorescence
• Sample Tested: HEK cells
• Species: Human

reviewed by: Verified Customer ICC/IF Canine 11/01/2016

Summary

• Application: Immunocytochemistry/Immunofluorescence
• Sample Tested: Madin-Darby Canine Kidney Epithelial Cells (MDCK Line)
• Species: Canine

Bioinformatics

• Gene Symbol: CD63
• Entrez:
  • 967(Human)
• Uniprot:
  • P08962()