CD63 Antibody (H5C6)

Images

 
Western Blot: CD63 Antibody (H5C6) [NBP2-42225] - THP1 whole cell protein was separated by SDS-PAGE on a 12% gel and transferred to PVDF membrane. The membrane was probed with anti-CD63 antibody at 2 ug/mL and detected ...read more
Immunocytochemistry/ Immunofluorescence: CD63 Antibody (H5C6) [NBP2-42225] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Immunocytochemistry/ Immunofluorescence: CD63 Antibody (H5C6) [NBP2-42225] - The CD63 (H5C6) antibody was tested in HeLa cells at a 1:50 dilution against DyLight 488 (Green). Actin and nuclei were counterstained against ...read more
Flow Cytometry: CD63 Antibody (H5C6) [NBP2-42225] - An intracellular stain was performed on SK-MEL-28 cells with CD63 (H5C6) Antibody NBP2-42225R (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more
Immunocytochemistry/ Immunofluorescence: CD63 Antibody (H5C6) [NBP2-42225] - MDCK cells stained with CD63 antibody at a dilution of 1:50 followed by Donkey anti-mouse secondary antibody conjugated with Alexa Fluor 488 ...read more
Immunocytochemistry/ Immunofluorescence: CD63 Antibody (H5C6) [NBP2-42225] - U2OS cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Immunocytochemistry/ Immunofluorescence: CD63 Antibody (H5C6) [NBP2-42225] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Immunocytochemistry/ Immunofluorescence: CD63 Antibody (H5C6) [NBP2-42225] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Immunocytochemistry/ Immunofluorescence: CD63 Antibody (H5C6) [NBP2-42225] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells ...read more
Western Blot: CD63 Antibody (H5C6) [NBP2-42225] - The same samples and volumes were run under reducing and non-reducing conditions. All procedures were performed in parallel for both conditions. L- ladder +- exosomes. ...read more
Immunocytochemistry/ Immunofluorescence: CD63 Antibody (H5C6) [NBP2-42225] - HEK cells stained with CD63 antibody at a dilution of 1:50 followed by Donkey anti-mouse secondary antibody conjugated with Alexa Fluor 488 ...read more
Flow Cytometry: CD63 Antibody (H5C6) [NBP2-42225] - Human peripheral blood cells were stained (2 x 10^6 cells/mL) using the anti-CD63 antibody (Blue) at a dilution of 1:1000. Signal was detected using a Gt x Ms DyLight ...read more
Flow Cytometry: CD63 Antibody (H5C6) [NBP2-42225] - A431 cells were stained (1 x10^6 cells/mL) using the anti-CD63 antibody at a 1:1000 dilution (blue). Signal was detected with Gt x Ms DyLight 488 secondary. Isotype ...read more
Flow (Intracellular): CD63 Antibody (H5C6) [NBP2-42225] - An intracellular stain was performed on HepG2 cells with CD63 Antibody (H5C6) NBP2-42225AF488 (blue) and a matched isotype control (orange). Cells were fixed ...read more
Flow Cytometry: CD63 Antibody (H5C6) [NBP2-42225] - An intracellular stain was performed on HeLa cells with CD63 Antibody (H5C6) NBP2-42225APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA ...read more
Flow Cytometry: CD63 Antibody (H5C6) [NBP2-42225] - An intracellular stain was performed on SK-MEL-28 cells with CD63 Antibody (H5C6) NBP2-42225AF488 (blue) and a matched isotype control (orange). Cells were fixed with ...read more
Flow Cytometry: CD63 Antibody (H5C6) [NBP2-42225] - An intracellular stain was performed on HeLa cells with CD63 [H5C6] Antibody NBP2-42225AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more

Product Details

Summary
Reactivity Hu, CaSpecies Glossary
Applications WB, DB, ELISA, EM, Flow, Flow-IC, Func, ICC/IF, In vitro, IP, IHC-WhMt
Clone
H5C6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

CD63 Antibody (H5C6) Summary

Immunogen
Human splenic adherent cells.
Marker
Exosome Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD63
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Dot Blot
  • Electron Microscopy
  • ELISA
  • Flow (Intracellular)
  • Flow Cytometry 1:1000
  • Functional
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunohistochemistry Whole-Mount
  • Immunoprecipitation
  • In vitro assay
  • Western Blot
Application Notes
Use in Electron Microscopy reported in scientific literature (PMID: 16735575). Use in functional reported in scientific literature (PMID: 9811687). Use in In vitro and Immunohistochemistry-Whole mount reported in scientific literature (PMID: 21464080).
Reviewed Applications
Read 5 Reviews rated 4
using
NBP2-42225 in the following applications:

Publications
Read Publications using
NBP2-42225 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CD63 Antibody (H5C6)

  • CD_antigen: CD63
  • CD63 antigen (melanoma 1 antigen)
  • CD63 antigen
  • CD63 molecule
  • CD63
  • Granulophysin
  • Lamp-3
  • Lysosomal-associated membrane protein 3
  • lysosome-associated membrane glycoprotein 3
  • ME491
  • melanoma 1 antigen
  • Melanoma-associated antigen ME491
  • melanoma-associated antigen MLA1
  • MLA1
  • Ocular melanoma-associated antigen
  • OMA81H
  • tetraspanin-30
  • Tspan30
  • tspan-30

Background

CD63 (cluster of differentiation 63), also known as lysosome associated membrane protein 3 (LAMP-3), is a membrane spanning glycoprotein with a molecular mass ranging from 30-60 kDa that was the first characterized member of the tetraspanin superfamily (1,2). CD63 is ubiquitously expressed and largely present on the cell surface in the endosomal system (1-3). More specifically, it is generally present in multivesicular bodies (MVBs), also called late endosomes, and lysosomes (1). The CD63 molecule is a total of 238 amino acids (aa), has four hydrophobic membrane-spanning regions and three N-glycosylation sites, and the encoded protein has a theoretical molecular weight of 25 kDa (2,3). CD63 both directly and indirectly interacts with other proteins including integrins, cell surface receptors, other tetraspanins, kinases, and adapter proteins, to name a few (1). CD63 is involved in many cell processes including cell survival and activation, cell adhesion, invasion, and migration (1). Additionally, CD63 has been shown to interact with tissue inhibitor of metalloproteinase 1 (TIMP-1), which originally thought to be an inhibitor of cancer progression has recently been shown to have cancer promoting properties as well (1,4). The CD63-TIMP-1 interaction has been shown to activate the PI3K/AKT pathway in lung adenocarcinoma cells and also promote survival and invasion of acute myeloid leukemia cells (1).CD63 is a useful flow cytometry marker for basophil granulocytes and can be used for the basophil activation test (BAT) to assess IgE-mediated allergy response (5). As CD63 was initially discovered on activated blood platelets and is a lysosomal-associated protein it makes sense it would be involved in platelet or lysosomal-related disorders (1,2). More precisely, CD63 is associated with Hermansky-Pudlak syndrome, a disease characterized by albinism and platelet storage pool deficiency (6).

References

1. Pols, M. S., & Klumperman, J. (2009). Trafficking and function of the tetraspanin CD63. Experimental cell research. https://doi.org/10.1016/j.yexcr.2008.09.020

2. Metzelaar, M. J., Wijngaard, P. L., Peters, P. J., Sixma, J. J., Nieuwenhuis, H. K., & Clevers, H. C. (1991). CD63 antigen. A novel lysosomal membrane glycoprotein, cloned by a screening procedure for intracellular antigens in eukaryotic cells. The Journal of biological chemistry.

3. Horejsi, V., & Vlcek, C. (1991). Novel structurally distinct family of leucocyte surface glycoproteins including CD9, CD37, CD53 and CD63. FEBS letters. https://doi.org/10.1016/0014-5793(91)80988-f

4. Eckfeld, C., HauBler, D., Schoeps, B., Hermann, C. D., & Kruger, A. (2019). Functional disparities within the TIMP family in cancer: hints from molecular divergence. Cancer metastasis reviews. https://doi.org/10.1007/s10555-019-09812-6

5. Hoffmann, H. J., Santos, A. F., Mayorga, C., Nopp, A., Eberlein, B., Ferrer, M., Rouzaire, P., Ebo, D. G., Sabato, V., Sanz, M. L., Pecaric-Petkovic, T., Patil, S. U., Hausmann, O. V., Shreffler, W. G., Korosec, P., & Knol, E. F. (2015). The clinical utility of basophil activation testing in diagnosis and monitoring of allergic disease. Allergy. https://doi.org/10.1111/all.12698

6. Dell'Angelica, E. C., Shotelersuk, V., Aguilar, R. C., Gahl, W. A., & Bonifacino, J. S. (1999). Altered trafficking of lysosomal proteins in Hermansky-Pudlak syndrome due to mutations in the beta 3A subunit of the AP-3 adaptor. Molecular cell. https://doi.org/10.1016/s1097-2765(00)80170-7

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD63 Antibody (NBP2-42225)(30)

We have publications tested in 2 confirmed species: Human, Canine.

We have publications tested in 10 applications: DB, ELISA, EM, FLOW, Func, ICC/IF, IP, In vitro, WB, in vitro.


Filter By Application
DB
(1)
ELISA
(2)
EM
(2)
FLOW
(4)
Func
(3)
ICC/IF
(5)
IP
(2)
In vitro
(2)
WB
(11)
in vitro
(1)
All Applications
Filter By Species
Human
(17)
Canine
(2)
All Species
Showing Publications 1 - 10 of 30. Show All 30 Publications.
Publications using NBP2-42225 Applications Species
Lee C, Chen Y, Hsiao A Et al. Demystifying the long noncoding RNA landscape of small EVs derived from human mesenchymal stromal cells Journal of Advanced Research Nov 1 2021 (WB, Human) WB Human
Leroux E, Perbet R, Caillierez R Et al. Extracellular vesicles: Major actors of heterogeneity in tau spreading among human tauopathies Molecular therapy : the journal of the American Society of Gene Therapy Sep 24 2021 [PMID: 34563677] (EM, Human) EM Human
Lee J, Lee SA, Gu NY et al. Canine Natural Killer Cell-Derived Exosomes Exhibit Antitumor Activity in a Mouse Model of Canine Mammary Tumor BioMed research international Sep 4 2021 [PMID: 34527741] (WB, Canine) WB Canine
Teshima T, Yuchi Y, Suzuki R Et al. Immunomodulatory Effects of Canine Adipose Tissue Mesenchymal Stem Cell-Derived Extracellular Vesicles on Stimulated CD4+ T Cells Isolated from Peripheral Blood Mononuclear Cells Journal of immunology research Aug 14 2021 [PMID: 34447855]
Wang J, Wuethrich A, Lobb RJ et al. Characterizing the Heterogeneity of Small Extracellular Vesicle Populations in Multiple Cancer Types via an Ultrasensitive Chip ACS sensors Jul 15 2021 [PMID: 34264628]
Pederson PJ, Liang H, Filonov D, Mooberry SL. Eribulin and Paclitaxel Differentially Alter Extracellular Vesicles and Their Cargo from Triple-Negative Breast Cancer Cells Cancers (Basel) Jul 2 2021 [PMID: 34205051]

Details:
This citation used the Alexa Fluor 488 format of this antibody.
Krishnamachary B, Mahajan A, Kumar A et al. Extracellular vesicle TGF-beta 1 is linked to cardiopulmonary dysfunction in HIV American journal of respiratory cell and molecular biology May 20 2021 [PMID: 34014809]
Wang J, Wuethrich A, Sina AA et al. Tracking extracellular vesicle phenotypic changes enables treatment monitoring in melanoma Sci Adv Feb 1 2020 [PMID: 32133394] (WB, Human) WB Human
Wright AB The isolation, culture-expansion, cryopreservation, characterization, and properties of umbilical cord-derived mesenchymal stromal cells and their extracellular vesicles Thesis Jan 1 2021
Arab T, Mallick ER, Huang Y et al. Characterization of extracellular vesicles and synthetic nanoparticles with four orthogonal single-particle analysis platforms Journal of extracellular vesicles Apr 1 2021 [PMID: 33850608]
Show All 30 Publications.

Reviews for CD63 Antibody (NBP2-42225) (5) 45

Average Rating: 4
(Based on 5 reviews)
We have 5 reviews tested in 2 species: Human, Canine.

Reviews using NBP2-42225:
Filter by Applications
DB
(1)
WB
(2)
ICC/IF
(2)
All Applications
Filter by Species
Human
(3)
Canine
(2)
All Species
Images Ratings Applications Species Date Details
Dot Blot CD63 NBP2-42225
Enlarge
5
reviewed by:
Verified Customer
DB Canine 10/09/2020
View

Summary

ApplicationDot Blot
Sample Testedcell lysate canine umbilical cord-derived mesenchymal stromal cells
SpeciesCanine
LotC-2
Western Blot CD63 NBP2-42225
Enlarge
3
reviewed by:
Verified Customer
WB Human 10/02/2019
View

Summary

ApplicationWestern Blot
Sample Tested Hela whole cell lysate
SpeciesHuman
LotB-4
Western Blot CD63 NBP2-42225
Enlarge
5
reviewed by:
Verified Customer
WB Human 09/18/2017
View

Summary

ApplicationWestern Blot
Sample TestedBlood plasma,urine
SpeciesHuman
LotA-1

Comments

CommentsAntibody labelled with HRP
Immunocytochemistry/Immunofluorescence CD63 NBP2-42225
Enlarge
3
reviewed by:
Sneha Bhat
ICC/IF Human 11/01/2016
View

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedHEK cells
SpeciesHuman
Immunocytochemistry/Immunofluorescence CD63 NBP2-42225
Enlarge
4
reviewed by:
Sneha Bhat
ICC/IF Canine 11/01/2016
View

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedMadin-Darby Canine Kidney Epithelial Cells (MDCK Line)
SpeciesCanine

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD63 Antibody (NBP2-42225) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-42225AF350
Alexa Fluor 405 NBP2-42225AF405
Alexa Fluor 488 NBP2-42225AF488
Alexa Fluor 532 NBP2-42225AF532
Alexa Fluor 594 NBP2-42225AF594
Alexa Fluor 647 NBP2-42225AF647
Alexa Fluor 700 NBP2-42225AF700
Alexa Fluor 750 NBP2-42225AF750
Allophycocyanin NBP2-42225APC
Allophycocyanin/Cy7 NBP2-42225APCCY7
Biotin NBP2-42225B
DyLight 350 NBP2-42225UV
DyLight 405 NBP2-42225V
DyLight 488 NBP2-42225G
DyLight 550 NBP2-42225R
DyLight 594 NBP2-42225DL594
DyLight 650 NBP2-42225C
DyLight 680 NBP2-42225FR
DyLight 755 NBP2-42225IR
HRP NBP2-42225H
Janelia Fluor 549 NBP2-42225JF549
Janelia Fluor 646 NBP2-42225JF646
PE NBP2-42225PE
PE/Atto594 NBP2-42225PEATT594
PE/Cy5.5 NBP2-42225PECY55
PE/Cy7 NBP2-42225PECY7
PerCP NBP2-42225PCP

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Blogs on CD63.

Glypican 3 as a biomarker for gastro-esophageal adenocarcinoma
By Jamshed Arslan, Pharm. D., PhD. Gastroesophageal adenocarcinoma originates from the glandular epithelium of the esophagus, gastroesophageal junction and stomach. The incidence of gastroesophageal adenocarcinoma is ...  Read full blog post.

Tools for Isolation, Quantification and Analysis of Exosomes
Exosomes are spherical to cup-shaped bilayered membrane enclosed nanosize vesicles (30-100 nm) which have the ability to shuttle active cargoes between cells. Johnstone et al. 1987 pioneered in documenting the generation of exosomes in differentiat...  Read full blog post.

CD63: is it pro-metastatic or anti-metastatic?
CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,...  Read full blog post.

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Recent Reviews

4
5
2
4
1
3
2
2
0
1
0

Verified Customer
10/09/2020
Application: DB
Species: Canine

Verified Customer
10/02/2019
Application: WB
Species: Human

Verified Customer
09/18/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol CD63
Entrez
Uniprot