TSPAN8/TM4SF3 Antibody (458811) [Unconjugated]

Images

 
Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line and HT‑29 human colon adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human TSPAN8 Monoclonal Antibody ...read more
HT‑29 human colon adenocarcinoma cell line was stained with Rat Anti-Human TSPAN8 Monoclonal Antibody (Catalog # MAB4734, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by ...read more
TSPAN8 was detected in immersion fixed HT‑29 human colon adenocarcinoma cell line using Rat Anti-Human TSPAN8 Monoclonal Antibody (Catalog # MAB4734) at 10 µg/mL for 3 hours at room temperature. Cells were stained ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ICC/IF
Clone
458811
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

TSPAN8/TM4SF3 Antibody (458811) [Unconjugated] Summary

Immunogen
NS0 mouse myeloma cell line transfected with human TSPAN8
Met1-Lys237 (Ile35Val)
Accession # AAH05246
Specificity
Detects human TSPAN8 in Western blots.
Details of Functionality
ActivityAssaywCitation not found None None None
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
TSPAN8
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
Publications
Read Publication using
MAB4734 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TSPAN8/TM4SF3 Antibody (458811) [Unconjugated]

  • CO-029
  • tetraspanin 8
  • TM4SF3
  • TM4SF3tspan-8
  • Transmembrane 4 superfamily member 3tetraspanin-8
  • TSPAN8
  • Tspan-8
  • Tumor-associated antigen CO-029

Background

Tetraspanin-8 (TSPAN8), also known as TM4SF3 and human tumor-associated antigen CO-0029, is a member of the transmembrane 4 superfamily. It is a cell surface 27‑34 kDa glycoprotein with 4 transmembrane segments, and a large extracellular loop (LEL) domain from amino acids (aa) 106‑206. Both the N- and C-termini are intracellular. TSPAN8 is expressed by multiple tumor types as well as smooth and skeletal muscle cells, endothelial cells, hematopoietic progenitor cells and non-keratinized squamous epithelium. As with other TSPAN family molecules, TSPAN8 acts as an organizer of microdomains in membranes. Molecules that may be found in these domains include CD151 (another TSPAN family member), beta 1 Integrins, Integrin alpha 4 beta 6, EpCAM, and CD13. TSPAN8 would appear to remove Integrins from the cell surface, facilitating cell motility which, in the case of tumor cells, results in metastasis. TSPAN8 shares 53% aa sequence identity with both mouse and rat TSPAN8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB500-327
Species: Hu
Applications: WB, ELISA, Flow, IHC, IHC-P, In vitro, CyTOF-ready
NBP2-42225
Species: Hu, Ca
Applications: WB, DB, EM, ELISA, Flow, Func, ICC/IF, IP, In vitro, Flow-IC, IHC-WhMt
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP2-45041
Species: Hu, Po
Applications: Flow, IHC, IHC-Fr, IF
NB600-1182
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
NBP2-57403
Species: Hu
Applications: WB, ICC/IF
NBP2-93002
Species: Hu, Mu
Applications: WB, ICC/IF
NBP1-82916
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-28869
Species: Hu, Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P
NB100-64810
Species: Hu
Applications: WB, ELISA, Flow, IHC, IHC-Fr, IHC-P, IP
AF1190
Species: Rt
Applications: WB, Flow, IHC, Block, CyTOF-ready
NBP2-45600
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, IHC, IF
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-85589
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-21792
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
H00006934-M06
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB500-393
Species: Hu
Applications: WB, Flow, IHC, IHC-Fr, IP, CyTOF-ready
NBP1-89680
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, ChIP
MAB4734
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC/IF

Publications for TSPAN8/TM4SF3 Antibody (MAB4734)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Microarray.


Filter By Application
Microarray
(1)
All Applications
Filter By Species
Mouse
(1)
All Species

Reviews for TSPAN8/TM4SF3 Antibody (MAB4734) (0)

There are no reviews for TSPAN8/TM4SF3 Antibody (MAB4734). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TSPAN8/TM4SF3 Antibody (MAB4734) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB4734U
Alexa Fluor 405 FAB4734V
Alexa Fluor 488 FAB4734G
Alexa Fluor 594 FAB4734T
Alexa Fluor 647 FAB4734R
Alexa Fluor 700 FAB4734N
Alexa Fluor 750 FAB4734S
Phycoerythrin FAB4734P

Additional TSPAN8/TM4SF3 Products

Bioinformatics Tool for TSPAN8/TM4SF3 Antibody (MAB4734)

Discover related pathways, diseases and genes to TSPAN8/TM4SF3 Antibody (MAB4734). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TSPAN8/TM4SF3 Antibody (MAB4734)

Discover more about diseases related to TSPAN8/TM4SF3 Antibody (MAB4734).
 

Pathways for TSPAN8/TM4SF3 Antibody (MAB4734)

View related products by pathway.

PTMs for TSPAN8/TM4SF3 Antibody (MAB4734)

Learn more about PTMs related to TSPAN8/TM4SF3 Antibody (MAB4734).

Blogs on TSPAN8/TM4SF3

There are no specific blogs for TSPAN8/TM4SF3, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TSPAN8/TM4SF3 Antibody (458811) [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol TSPAN8
Uniprot