Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line and HT‑29 human colon adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human TSPAN8 Monoclonal Antibody ...read more
HT‑29 human colon adenocarcinoma cell line was stained with Rat Anti-Human TSPAN8 Monoclonal Antibody (Catalog # MAB4734, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by ...read more
TSPAN8 was detected in immersion fixed HT‑29 human colon adenocarcinoma cell line using Rat Anti-Human TSPAN8 Monoclonal Antibody (Catalog # MAB4734) at 10 µg/mL for 3 hours at room temperature. Cells were stained ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.5 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for TSPAN8/TM4SF3 Antibody (458811) [Unconjugated]
Transmembrane 4 superfamily member 3tetraspanin-8
Tumor-associated antigen CO-029
Tetraspanin-8 (TSPAN8), also known as TM4SF3 and human tumor-associated antigen CO-0029, is a member of the transmembrane 4 superfamily. It is a cell surface 27‑34 kDa glycoprotein with 4 transmembrane segments, and a large extracellular loop (LEL) domain from amino acids (aa) 106‑206. Both the N- and C-termini are intracellular. TSPAN8 is expressed by multiple tumor types as well as smooth and skeletal muscle cells, endothelial cells, hematopoietic progenitor cells and non-keratinized squamous epithelium. As with other TSPAN family molecules, TSPAN8 acts as an organizer of microdomains in membranes. Molecules that may be found in these domains include CD151 (another TSPAN family member), beta 1 Integrins, Integrin alpha 4 beta 6, EpCAM, and CD13. TSPAN8 would appear to remove Integrins from the cell surface, facilitating cell motility which, in the case of tumor cells, results in metastasis. TSPAN8 shares 53% aa sequence identity with both mouse and rat TSPAN8.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.