CD19 Antibody (CB19)

Images

 
Immunocytochemistry/ Immunofluorescence: CD19 Antibody (CB19) [NBP2-25196] - The CD19 (CB19) antibody was tested in Daudi cells at a 1:40 dilution against Dylight 488 (Green). Actin and nuclei were counterstained ...read more
Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - A surface stain was performed on human peripheral blood lymphocytes with CD9 (HI9a) antibody NB100-77915AF647 and a matched isotype control NBP2-27287AF488. Cells were ...read more
Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Surface staining of 10^6 human lymphocytes using 0.2 ug of CD19 antibody (red) and isotype control (green). Cell surface staining kit was used for this test.
Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Analysis using the FITC conjugate of NBP2-25196. Staining of CD19 in 10^6 human lymphocytes using 10 ul (0.25 ug) of this antibody. Shaded histogram represents cells ...read more
Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Analysis using the PE conjugate of NBP2-25196. Staining of CD19 in 1x10^6 human PBMC using 10 ul (0.25 ug) of was used to test this product. Propidium iodide negative ...read more
Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-25196. Staining of human PBMC. Image from verified customer review.
Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Analysis using the DyLight 405 conjugate of NBP2-25196. Staining of CD19 in human PBMCs using anti-CD19 antibody. Image from verified customer review.
Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - A surface stain was performed on human peripheral blood lymphocytes with TACI/TNFRSF13B/CVID (1A1) antibody NB600-1189PE and a matched isotype control. Cells were ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, In vitro, CyTOF-ready
Clone
CB19
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

CD19 Antibody (CB19) Summary

Immunogen
Intact normal human B cell cells were used as immunogen to generate the CB19 clone.
Epitope
The exact CD19 epitope recognized by the antibody has not been mapped and is unknown.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD19
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry 0.2 ug/10^6 cells
  • Immunocytochemistry/Immunofluorescence 1:25-1:100
  • Immunohistochemistry 1:10-1:500
  • In vitro assay
  • CyTOF-ready
Application Notes
Use in Western blot reported in scientific literature (PMID17327405). Use in In vitro reported in scientific literature (PMID 9576485)
Publications
Read Publications using
NBP2-25196 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27532872).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CD19 Antibody (CB19)

  • B4
  • B-lymphocyte antigen CD19
  • B-lymphocyte surface antigen B4
  • CD19 antigen
  • CD19 molecule
  • CD19
  • CVID3
  • Differentiation antigen CD19
  • Leu-12
  • MGC12802
  • T-cell surface antigen Leu-12

Background

CD19 is a member of the immunoglobulin superfamily with 556 amino acid and two immunoglobulin-like C2-type domains. As a cell surface protein, CD19 is known to form a complex with CD21, CD81 and CD225 in the membrane of mature B cells. A major function of CD19 is to assemble with the antigen receptor of B lymphocytes so as to decrease the threshold for antigen receptor dependent stimulation thus enhancing the specificity and sensitivity of B cells towards antigens. CD19 is an important protein for B cell antigen receptor signaling and regulation and also acts as a specialized adaptor protein for the amplification of Src family needed for this purpose. Regulation of growth of B cells is a major function of CD19 and its expression is confined to only B lymphocytes and follicular dendritic cells of the hematopoietic system. Increased expression of CD19 induces the production of autoantibody thus giving an insight to the regulatory role of CD19 in autoimmunity. Defects in CD19 are a cause of hypogammaglobulinemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD19 Antibody (NBP2-25196)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: FLOW, ICC/IF, In vitro, WB.


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FLOW
(3)
ICC/IF
(4)
In vitro
(1)
WB
(1)
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Human
(7)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 10.
Publications using NBP2-25196 Applications Species
Holla S, Prakhar P, Singh V et al. MUSASHI-Mediated Expression of JMJD3, a H3K27me3 Demethylase, Is Involved in Foamy Macrophage Generation during Mycobacterial Infection PLoS Pathog. PLoS Pathog. 2016 Aug 1 [PMID: 27532872] (Mouse)

Details:
This citation used the PE version of this antibody.
Mouse
Deaglio S, Vaisitti T, Billington R et al. CD38/CD19: a lipid raft-dependent signaling complex in human B cells Blood. 2007 Jun 15 [PMID: 17327405] (WB, ICC/IF, Human) WB, ICC/IF Human
Deaglio S, Vaisitti T, Bergui L et al. CD38 and CD100 lead a network of surface receptors relaying positive signals for B-CLL growth and survival Blood. 2005 Apr 15 [PMID: 15613544] (FLOW, ICC/IF, Human) FLOW, ICC/IF Human
Deaglio S, Capobianco A, Bergui L et al. CD38 is a signaling molecule in B-cell chronic lymphocytic leukemia cells Blood. 2003 Sep 15 [PMID: 12763926] (FLOW, ICC/IF, Human) FLOW, ICC/IF Human
Musso T, Deaglio S, Franco L et al. CD38 expression and functional activities are up-regulated by IFN-gamma on human monocytes and monocytic cell lines J Leukoc Biol. 2001 Apr 1 [PMID: 11310847] (Human) Human
Deaglio S, Canella D, Baj G et al. Evidence of an immunologic mechanism behind the therapeutical effects of arsenic trioxide (As(2)O(3)) on myeloma cells Leuk Res. 2001 Mar 1 [PMID: 11226519]
Zilber MT, Gregory S, Mallone R et al. CD38 expressed on human monocytes: a coaccessory molecule in the superantigen-induced proliferation Proc Natl Acad Sci U S A. 2000 Mar 14 [PMID: 10706632] (Human) Human
Morra M, Zubiaur M, Terhorst C et al. CD38 is functionally dependent on the TCR/CD3 complex in human T cells FASEB J. 1998 May 1 [PMID: 9576485] (In vitro, Human) In vitro Human
Deaglio S, Morra M, Mallone R et al. Human CD38 (ADP-ribosyl cyclase) is a counter-receptor of CD31, an Ig superfamily member J Immunol. 1998 Jan 1 [PMID: 9551996] (FLOW, Human) FLOW Human
Wang X, Nakamoto T, Dulinska-Molak I. Regulating the stemness of mesenchymal stem cells by tuning micropattern features. Journal of Materials Chemistry B. 2015 Nov 12 (ICC/IF) ICC/IF

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD19 Antibody (NBP2-25196) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-25196AF405
Alexa Fluor 488 Labeled NBP2-25196AF488
Alexa Fluor 647 Labeled NBP2-25196AF647
Alexa Fluor 700 Labeled NBP2-25196AF700
Allophycocyanin Labeled NBP2-25196APC
Biotin Labeled NBP2-25196B
DyLight 350 Labeled NBP2-25196UV
DyLight 405 Labeled NBP2-25196V
DyLight 488 Labeled NBP2-25196G
DyLight 550 Labeled NBP2-25196R
DyLight 650 Labeled NBP2-25196C
DyLight 680 Labeled NBP2-25196FR
DyLight 755 Labeled NBP2-25196IR
HRP Labeled NBP2-25196H
PE Labeled NBP2-26646
PerCP Labeled NBP2-25196PCP

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Blogs on CD19.

CD19: An Undoubted Biomarker for B Cells
CD19 is a cell surface protein member of the large immunoglobulin superfamily that complexes with CD21, CD81, and CD225 in the membrane of mature B-cells. A major function of CD19 is to assemble with the antigen receptor of B-lymphocytes to decrease t...  Read full blog post.

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Bioinformatics

Gene Symbol CD19
Entrez
Uniprot