CD19 Antibody (CB19)


Immunocytochemistry/ Immunofluorescence: CD19 Antibody (CB19) [NBP2-25196] - The CD19 (CB19) antibody was tested in Daudi cells at a 1:40 dilution against Dylight 488 (Green). Actin and nuclei were counterstained more
Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - A surface stain was performed on Ramos cells with CD19 Antibody (CB19) NBP2-26646 (blue) and a matched isotype control (orange). Cells were incubated in an antibody more
Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Surface staining of 10^6 human lymphocytes using 0.2 ug of CD19 antibody (red) and isotype control (green). Cell surface staining kit was used for this test.
Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Analysis using the FITC conjugate of NBP2-25196. Staining of CD19 in 10^6 human lymphocytes using 10 ul (0.25 ug) of this antibody. Shaded histogram represents cells more
Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Analysis using the PE conjugate of NBP2-25196. Staining of CD19 in 1x10^6 human PBMC using 10 ul (0.25 ug) of was used to test this product. Propidium iodide negative more
Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-25196. Staining of human PBMC. Image from verified customer review.
Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Analysis using the DyLight 405 conjugate of NBP2-25196. Staining of CD19 in human PBMCs using anti-CD19 antibody. Image from verified customer review.
Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - A surface stain was performed on human peripheral blood lymphocytes with TACI/TNFRSF13B/CVID (1A1) antibody NB600-1189PE and a matched isotype control. Cells were more
Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - A surface stain was performed on human peripheral blood lymphocytes with CD9 (HI9a) antibody NB100-77915AF647 and a matched isotype control NBP2-27287AF488. Cells were more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, In vitro, CyTOF-ready
1.0 mg/ml

Order Details

CD19 Antibody (CB19) Summary

Intact normal human B cell cells were used as immunogen to generate the CB19 clone.
The exact CD19 epitope recognized by the antibody has not been mapped and is unknown.
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • CyTOF-ready
  • Flow Cytometry 0.2 ug/10^6 cells
  • Immunocytochemistry/Immunofluorescence 1:25-1:100
  • In vitro assay
  • Western Blot
Application Notes
Use in WB reported in scientific literature (PMID17327405). Use in In vitro reported in scientific literature (PMID 9576485). This antibody is CyTOF ready.
Read Publications using
NBP2-25196 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27532872).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for CD19 Antibody (CB19)

  • B4
  • B-lymphocyte antigen CD19
  • B-lymphocyte surface antigen B4
  • CD19 antigen
  • CD19 molecule
  • CD19
  • CVID3
  • Differentiation antigen CD19
  • Leu-12
  • MGC12802
  • T-cell surface antigen Leu-12


CD19 is a member of the immunoglobulin superfamily with 556 amino acid and two immunoglobulin-like C2-type domains. As a cell surface protein, CD19 is known to form a complex with CD21, CD81 and CD225 in the membrane of mature B cells. A major function of CD19 is to assemble with the antigen receptor of B lymphocytes so as to decrease the threshold for antigen receptor dependent stimulation thus enhancing the specificity and sensitivity of B cells towards antigens. CD19 is an important protein for B cell antigen receptor signaling and regulation and also acts as a specialized adaptor protein for the amplification of Src family needed for this purpose. Regulation of growth of B cells is a major function of CD19 and its expression is confined to only B lymphocytes and follicular dendritic cells of the hematopoietic system. Increased expression of CD19 induces the production of autoantibody thus giving an insight to the regulatory role of CD19 in autoimmunity. Defects in CD19 are a cause of hypogammaglobulinemia.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD19 Antibody (NBP2-25196)(11)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: FLOW, ICC/IF, In vitro, WB.

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In vitro
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP2-25196 Applications Species
Deaglio S, Vaisitti T, Billington R et al. CD38/CD19: a lipid raft-dependent signaling complex in human B cells Blood. 2007 Jun 15 [PMID: 17327405] (WB, ICC/IF, Human) WB, ICC/IF Human
Deaglio S, Vaisitti T, Bergui L et al. CD38 and CD100 lead a network of surface receptors relaying positive signals for B-CLL growth and survival Blood. 2005 Apr 15 [PMID: 15613544] (FLOW, ICC/IF, Human) FLOW, ICC/IF Human
Deaglio S, Capobianco A, Bergui L et al. CD38 is a signaling molecule in B-cell chronic lymphocytic leukemia cells Blood. 2003 Sep 15 [PMID: 12763926] (FLOW, ICC/IF, Human) FLOW, ICC/IF Human
Musso T, Deaglio S, Franco L et al. CD38 expression and functional activities are up-regulated by IFN-gamma on human monocytes and monocytic cell lines J Leukoc Biol. 2001 Apr 1 [PMID: 11310847] (Human) Human
Deaglio S, Canella D, Baj G et al. Evidence of an immunologic mechanism behind the therapeutical effects of arsenic trioxide (As(2)O(3)) on myeloma cells Leuk Res. 2001 Mar 1 [PMID: 11226519]
Zilber MT, Gregory S, Mallone R et al. CD38 expressed on human monocytes: a coaccessory molecule in the superantigen-induced proliferation Proc Natl Acad Sci U S A. 2000 Mar 14 [PMID: 10706632] (Human) Human
Morra M, Zubiaur M, Terhorst C et al. CD38 is functionally dependent on the TCR/CD3 complex in human T cells FASEB J. 1998 May 1 [PMID: 9576485] (In vitro, Human) In vitro Human
Deaglio S, Morra M, Mallone R et al. Human CD38 (ADP-ribosyl cyclase) is a counter-receptor of CD31, an Ig superfamily member J Immunol. 1998 Jan 1 [PMID: 9551996] (FLOW, Human) FLOW Human
Wang X, Nakamoto T, Dulinska-Molak I. Regulating the stemness of mesenchymal stem cells by tuning micropattern features. Journal of Materials Chemistry B. 2015 Nov 12 (ICC/IF) ICC/IF
Holla S, Prakhar P, Singh V et al. MUSASHI-Mediated Expression of JMJD3, a H3K27me3 Demethylase, Is Involved in Foamy Macrophage Generation during Mycobacterial Infection PLoS Pathog. PLoS Pathog. 2016 Aug 1 [PMID: 27532872] (Mouse)

This citation used the PE version of this antibody.
Show All 11 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD19 Antibody (NBP2-25196) (0)

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-25196AF350
Alexa Fluor 405 NBP2-25196AF405
Alexa Fluor 488 NBP2-25196AF488
Alexa Fluor 532 NBP2-25196AF532
Alexa Fluor 594 NBP2-25196AF594
Alexa Fluor 647 NBP2-25196AF647
Alexa Fluor 700 NBP2-25196AF700
Alexa Fluor 750 NBP2-25196AF750
Allophycocyanin NBP2-25196APC
Allophycocyanin/Cy7 NBP2-25196APCCY7
Biotin NBP2-25196B
DyLight 350 NBP2-25196UV
DyLight 405 NBP2-25196V
DyLight 488 NBP2-25196G
DyLight 550 NBP2-25196R
DyLight 594 NBP2-25196DL594
DyLight 650 NBP2-25196C
DyLight 680 NBP2-25196FR
DyLight 755 NBP2-25196IR
FITC NBP2-25196F
HRP NBP2-25196H
Janelia Fluor 549 NBP2-25196JF549
Janelia Fluor 646 NBP2-25196JF646
PE NBP2-26646
PE/Atto594 NBP2-25196PEATT594
PE/Cy5.5 NBP2-25196PECY55
PE/Cy7 NBP2-25196PECY7
PerCP NBP2-25196PCP

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Harnessing Natural Killer Cell Activity for Anti-Tumor Immunotherapy
By Victoria Osinski, PhDWhat’s “Natural” About Natural Killer (NK) Cells?For immunologists, the term cytotoxicity often conjures up images of an army of antigen specific CD8+ T cells deploying to ...  Read full blog post.

How to identify B cell subsets using flow cytometry
By Victoria OsinskiUsing flow cytometry to identify B cell subsetsIdentifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified a...  Read full blog post.

Antigen-loss relapse after successful CAR-T therapy; What do we do now?
By Jacqueline Carrico, BS, MD Tumor cell mechanisms driving tolerance to CAR-T Despite very promising results of CAR-T therapy in acute lymphoblastic leukemia, B-ALL, antigen-loss relapse has arisen as a major chall...  Read full blog post.

CD19: An Undoubted Biomarker for B Cells
CD19 is a cell surface protein member of the large immunoglobulin superfamily that complexes with CD21, CD81, and CD225 in the membrane of mature B-cells. A major function of CD19 is to assemble with the antigen receptor of B-lymphocytes to decrease t...  Read full blog post.

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Gene Symbol CD19