Siglec-2/CD22 Antibody (OTI1F12)

Western Blot: Siglec-2/CD22 Antibody (1F12) [NBP2-46125] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD22.

Immunocytochemistry/Immunofluorescence: Siglec-2/CD22 Antibody (OTI1F12) [NBP2-46125] - Staining in canine blood cells from a verified customer review.

Western Blot: Siglec-2/CD22 Antibody (1F12) [NBP2-46125] - Analysis of extracts (35ug) from 9 different cell lines by using CD22 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunocytochemistry/Immunofluorescence: Siglec-2/CD22 Antibody (OTI1F12) [NBP2-46125] - Siglec-2/CD22 Antibody (1F12) [NBP2-46125] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY CD22.

Immunocytochemistry/Immunofluorescence: Siglec-2/CD22 Antibody (OTI1F12) [NBP2-46125] - Siglec-2/CD22 Antibody (1F12) [NBP2-46125] - Analysis of HeLa cells.

• Reactivity: Hu, Mu, Rt, Ca, Pm
• Applications: WB, ICC/IF
• Clone: OTI1F12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Siglec-2/CD22 Antibody (OTI1F12) Summary

• Immunogen: Full length human recombinant protein of human CD22(NP_001762) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD22
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100
  • Western Blot 1:400-4000
• Application Notes: ICC/IF from a verified customer review.

Reactivity Notes

Canine reported in verified customer review. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Siglec-2/CD22 Antibody (OTI1F12)

• B-cell receptor CD22
• BL-CAM
• B-lymphocyte cell adhesion molecule
• CD22 antigenMGC130020
• CD22 molecule
• CD22
• sialic acid binding Ig-like lectin 2
• Sialic acid-binding Ig-like lectin 2
• Siglec2
• Siglec-2
• SIGLEC2FLJ22814
• T-cell surface antigen Leu-14

Background

CD22 is a single-pass type 1 membrane glycoprotein found in the cytoplasm and cell membrane of B-lymphocytes. CD22 has at least two isoforms (alpha and beta) produced by alternate splicing. The protein exists predominantly as a monomer of the beta isoform (95 kDa). It is also found as a heterodimer of the beta and alpha isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Siglec-2/CD22 Antibody (NBP2-46125) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Canine.

reviewed by: A G ICC Canine 06/13/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: blood cell slides
• Species: Canine
• Lot: A003

FAQs for Siglec-2/CD22 Antibody (NBP2-46125). (Showing 1 - 1 of 1 FAQ).

1. I am interested in buying a CD22 antibody for fluorescence microscopy and FACS I red on your web page that you also offer to label your antibodies, if required, is that correct?
   • We do have the ability to perform custom conjugations on certain antibodies, including some to the CD22 protein. If you send me the conjugate you are interested in I can provide you with a quote.

Bioinformatics

• Gene Symbol: CD22