CD31/PECAM-1 Antibody

Product Discontinued

CD31/PECAM-1 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 670-720 of Human CD31.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PECAM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Immunocytochemistry/Immunofluorescence was reported in scientific literature. IHYC Frozen reactivity reported in scientific literature (PMID: 22713363).
• Theoretical MW: 130 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CD31/PECAM-1 Antibody

• adhesion molecule
• CD31 antigen
• CD31
• CD31/EndoCAM
• EndoCAM
• FLJ34100
• FLJ58394
• GPIIA'
• PECA1
• PECAM1
• PECAM-1
• PECAM-1, CD31/EndoCAM
• platelet endothelial cell adhesion molecule
• platelet endothelial cell adhesion molecule-1
• platelet/endothelial cell adhesion molecule

Background

Cluster of differentiation (CD31), Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1), is a 30kDa type I transmembrane glycoprotein expressed by endothelial cells, platelets, leukocytes and some lymphocytes (e.g., B and T-cell subsets). Structurally, PECAM-1 consists of six extracellular immunoglobulin-like domains which serve to support various functions including homophilic binding, maintaining high expression of PECAM-1 within endothelial intercellular junctions, and supporting endothelial cell migration (1,2). PECAM's homophilic binding underscores its role in vascular barrier integrity and leukocyte trans-endothelial movement. However, PECAM may also engage in heterophilic interactions with molecules such as glycosaminoglycans (GAG), integrin alpha 5 beta 3, CD38 in lymphocytes and CD177/PR3 in neutrophils (2).

PECAM's intracellular cytoplasmic domain consists of a sequence of 118 amino acids and contains serine and tyrosine (also referred to as immunoreceptor tyrosine-based inhibitory motifs-ITIMs) residues, which may be phosphorylated upon cellular stimulation (3). ITIMs are phosphorylated by Src-family kinases and non-Src family kinases (e.g., Csk), leading to a conformational change which supports interactions with Src homology 2 (SH2) domain containing proteins such as protein-tyrosine phosphatase, SHP-2 (1,2). Formation of SHP-2/PECAM-1 complexes induces endothelial cell migration through the dephosphorylation of focal adhesion kinase and regulation of RhoA activity (1). Signaling downstream of ITIM tyrosine phosphorylations also plays a role in PECAM's anti-apoptotic activity, a function which is independent of its interaction with SHP-2. In platelets and leukocytes, phosphorylation of PECAM's cytosolic domain is inhibitory, preventing their activation.

References

1. Lertkiatmongkol, P., Liao, D., Mei, H., Hu, Y., & Newman, P. J. (2016). Endothelial functions of PECAM-1 (CD31). Current Opinion in Hematology. https://doi.org/10.1097/MOH.0000000000000239.Endothelial

2. Privratsky, J. R., & Newman, P. J. (2014). PECAM-1: Regulator of endothelial junctional integrity. Cell and Tissue Research. https://doi.org/10.1007/s00441-013-1779-3

3. Newman, P. J., & Newman, D. K. (2003). Signal transduction pathways mediated by PECAM-1: New roles for an old molecule in platelet and vascular cell biology. Arteriosclerosis, Thrombosis, and Vascular Biology. https://doi.org/10.1161/01.ATV.0000071347.69358.D9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD31/PECAM-1 Antibody (NB100-92205)(4)

Publications using NB100-92205

• Lee HJ, Jung YH, Choi GE et al. O-cyclic phytosphingosine-1-phosphate stimulates HIF1 alpha-dependent glycolytic reprogramming to enhance the therapeutic potential of mesenchymal stem cells Cell Death Dis 2019-08-05 [PMID: 31383843] (IF/IHC)
• Chou CW, Wang CC, Wu CP et al. Tumor cycling hypoxia induces chemoresistance in glioblastoma multiforme by upregulating the expression and function of ABCB1 Neuro Oncol 2012-09-03 [PMID: 22946104] (IF/IHC, Mouse)
• Kishore M, Ma L, Cornish G et al. Primed T Cell Responses to Chemokines Are Regulated by the Immunoglobulin-Like Molecule CD31 PLoS One 2012-01-01 [PMID: 22724015] (ICC/IF, Mouse)
• Hsieh CH, Wu CP, Lee HT et al. NADPH oxidase subunit 4 mediates cycling hypoxia-promoted radiation resistance in glioblastoma multiforme Free Radic Biol Med 2012-06-16 [PMID: 22713363] (ICC/IF, IHC-Fr, Mouse)

FAQs for CD31/PECAM-1 Antibody (NB100-92205). (Showing 1 - 1 of 1 FAQs).

1. I'm interested in purchasing primary CD31/PECAM1 antibody for immunohistochemistry staining on formalin fixed paraffin embedded mouse samples. You have two antibodies that would work for paraffin sections, NBP1-71663 and NB100-92205, and both are rabbit polyclonal antibody. Which one would you recommend I purchase?
   • I would recommend NBP1-71663 as the image is better than NB100-92205.

Bioinformatics

• Gene Symbol: PECAM1
• Entrez:
  • 5175(Human)
  • 18613(Mouse)
• Uniprot:
  • P16284()