CD31/PECAM-1 Antibody [Allophycocyanin]

Mouse splenocytes were stained with Goat Anti-Mouse/Rat CD31/PECAM-1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3628A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Rat splenocytes were stained with Goat Anti-Mouse/Rat CD31/PECAM-1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3628A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

CD31hiEmcnhi vessel and bone formation decreased during ageing but increased in bone regeneration. A, Representative images of CD31 (green), EMCN (red) and Osterix (white) immunostaining. Scale bar, 100 μm. G, growth plate. B, Quantification of CD31 and EMCN positive vessel volume in distal femora. C, Quantitative analysis of Osterix‐positive (OSX+) osteoprogenitors in distal femora. D and E, FACS analysis dot plot (D) and quantification (E) of CD31hiEMCNhi ECs. F, Representative images of CD31 (green) and EMCN (red) immunostaining in bone regeneration area after femoral trabecular bone ablation. Nuclei, DAPI (blue). Scale bar, 100 μm. G and H, Representative μCT images (G) and quantitative μCT analysis (H) of bone regeneration after femoral trabecular bone ablation. Selected areas for the measurements of bone volume (BV)/tissue volume (TV) were indicated with a yellow square. Data are shown as mean ± SD, (n = 6 in B, C, E and H). *P < .05; **P < .01 by one‐way ANOVA Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32080957), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu, Rt
• Applications: Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Allophycocyanin

CD31/PECAM-1 Antibody [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CD31/PECAM-1
  Glu18-Lys590
  Accession # Q08481
• Specificity: Detects mouse CD31/PECAM-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human CD31 and recombinant porcine CD31 is observed. Detects mouse CD31 and rat CD31 in flow cytometry.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PECAM1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD31/PECAM-1 Antibody [Allophycocyanin]

• adhesion molecule
• CD31 antigen
• CD31
• CD31/EndoCAM
• EndoCAM
• FLJ34100
• FLJ58394
• GPIIA'
• PECA1
• PECAM1
• PECAM-1
• PECAM-1, CD31/EndoCAM
• platelet endothelial cell adhesion molecule
• platelet endothelial cell adhesion molecule-1
• platelet/endothelial cell adhesion molecule

Background

PECAM-1 (Platelet-Endothelial Cell Adhesion Molecule-1), also known as CD31, is a 130 kDa type I transmembrane glycoprotein adhesion molecule in the immunoglobulin superfamily (1, 2). Expression is restricted to cells involved in circulation, especially endothelial cells, platelets, monocytes, neutrophils and lymphocyte subsets. PECAM-1 is concentrated at cell-cell junctions and is required for Transendothelial Migration (TEM) (1-3). The Extracellular Domain (ECD) of PECAM-1 has ten potential N-linked glycosylation sites and six C2-type Ig-like domains, the first of which is critical for adhesion and extravasation (3, 4). The cytoplasmic domain contains Immunoregulatory Tyrosine-based Inhibitory and Switch Motifs (ITIM, ITSM) that mediate both inhibition and activation via phosphotyrosine-mediated engagement of SH2-containing signaling molecules (1, 5). Metalloproteinase-mediated ectodomain shedding occurs during apoptosis (6) but increased serum PECAM-1 ectodomain in HIV and active multiple sclerosis occurs independent of apoptosis (7, 8). In humans, expression of six isoforms with exon deletions in the cytoplasmic domain is tissue- and stage-specific, but full-length PECAM-1 is predominant. A form lacking the ITSM predominates in mouse (9). Mouse PECAM-1 ECD shows 77%, 63%, 63%, 63%, and 61% amino acid (aa) identity with rat, human, canine, porcine, and bovine PECAM-1, respectively. PECAM-1 participates with other adhesion molecules in some functions, but is the critical molecule for TEM. Homotypic PECAM-1 adhesion in trans, combined with cycling of PECAM-1 to and from surface-connected endothelial cell vesicles, leads leukocytes across endothelial tight junctions (3, 10). Homotypic adhesion and signaling functions also strongly suppress mitochondria-dependent apoptosis (11). In platelets, PECAM-1 is necessary for limiting thrombus formation (12) and promoting integrin-mediated clot retraction and platelet spreading (13), but mechanisms for these phenomena are unclear. PECAM^-/- mice are deficient in chemokine-mediated chemotaxis (14).

1. Ilan, N. and J.A. Madri (2003) Curr. Opin. Cell Biol. 15:515.
2. Xie, Y. and W.A. Muller (1993) Proc. Natl. Acad. Sci. USA 90:5569.
3. Liao, F. et al. (1997) J. Exp. Med. 185:1349.
4. Nakada, M.T. et al. (2000) J. Immunol. 164:452.
5. Chemnitz, J.M. et al. (2004) J. Immunol. 173:945.
6. Ilan, N. et al. (2001) FASEB J. 15:362.
7. Eugenin, E.A. et al. (2006) J. Leukoc. Biol. 79:444.
8. Losy, J. et al. (1999) J. Neuroimmunol. 99:169.
9. Wang, Y. et al. (2003) Am. J. Physiol. Heart Circ. Physiol. 284:H1008.
10. Mamdouh, Z. et al. (2003) Nature 421:748.
11. Gao, C. et al. (2003) Blood 102:169.
12. Falati, S. et al. (2006) Blood 107:535.
13. Wee, J.L. and D.E. Jackson (2005) Blood 106:3816.
14. Wu, Y. et al. (2005) J. Immunol. 175:3484.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD31/PECAM-1 Antibody (FAB3628A)(11)

Publications using FAB3628A

• Park, H;Song, J;Jeong, HW;Grönloh, MLB;Koh, BI;Bovay, E;Kim, KP;Klotz, L;Thistlethwaite, PA;van Buul, JD;Sorokin, L;Adams, RH; Apelin modulates inflammation and leukocyte recruitment in experimental autoimmune encephalomyelitis Nature communications 2024-07-25 [PMID: 39060233] (Flow Cytometry, Mouse)
• Picchio, V;Gaetani, R;Pagano, F;Derevyanchuk, Y;Pagliarosi, O;Floris, E;Cozzolino, C;Bernava, G;Bordin, A;Rocha, F;Pereira, ARS;Ministro, A;Pinto, AT;De Falco, E;Serino, G;Massai, D;Tamarat, R;Pesce, M;Santos, SCR;Messina, E;Chimenti, I; Early Impairment of Paracrine and Phenotypic Features in Resident Cardiac Mesenchymal Stromal Cells after Thoracic Radiotherapy International journal of molecular sciences 2024-03-01 [PMID: 38474123] (Flow Cytometry, Rat)
• WZ He, M Yang, Y Jiang, C He, YC Sun, L Liu, M Huang, YR Jiao, KX Chen, J Hou, M Huang, YL Xu, X Feng, Y Liu, Q Guo, H Peng, Y Huang, T Su, Y Xiao, Y Li, C Zeng, G Lei, XH Luo, CJ Li miR-188-3p targets skeletal endothelium coupling of angiogenesis and osteogenesis during ageing Cell Death & Disease, 2022-05-25;13(5):494. 2022-05-25 [PMID: 35610206] (Flow Cytometry, Mouse)
• X Liu, Y Chai, G Liu, W Su, Q Guo, X Lv, P Gao, B Yu, G Ferbeyre, X Cao, M Wan Osteoclasts protect bone blood vessels against senescence through the angiogenin/plexin-B2 axis Nature Communications, 2021-03-23;12(1):1832. 2021-03-23 [PMID: 33758201] (Flow Cytometry, Mouse)
• Chen J, Lippo L, Labella R et al. Decreased blood vessel density and endothelial cell subset dynamics during ageing of the endocrine system Onco Targets Ther 11/10/2020 [PMID: 33215738]
• Mi Yang, Chang‐Jun Li, Ye Xiao, Qi Guo, Yan Huang, Tian Su et al. Ophiopogonin D promotes bone regeneration by stimulating CD31hi EMCNhi vessel formation Cell Proliferation 2020-03-01 [PMID: 32080957]
• Yang M, Guo Q, Peng H et al. Kruppel-like factor 3 inhibition by mutated lncRNA Reg1cp results in human high bone mass syndrome J. Exp. Med. 6/13/2019 [PMID: 31196982]
• M Yang, CJ Li, X Sun, Q Guo, Y Xiao, T Su, ML Tu, H Peng, Q Lu, Q Liu, HB He, TJ Jiang, MX Lei, M Wan, X Cao, XH Luo MiR-497?195 cluster regulates angiogenesis during coupling with osteogenesis by maintaining endothelial Notch and HIF-1? activity Nat Commun, 2017-07-07;8(0):16003. 2017-07-07 [PMID: 28685750] (Flow Cytometry, Mouse)
• UH Langen, ME Pitulescu, JM Kim, R Enriquez-G, KK Sivaraj, AP Kusumbe, A Singh, J Di Russo, MG Bixel, B Zhou, L Sorokin, JM Vaquerizas, RH Adams Cell-matrix signals specify bone endothelial cells during developmental osteogenesis Nat. Cell Biol, 2017-02-20;19(3):189-201. 2017-02-20 [PMID: 28218908] (Flow Cytometry, Mouse)
• AP Kusumbe, SK Ramasamy, T Itkin, MA Mäe, UH Langen, C Betsholtz, T Lapidot, RH Adams Age-dependent modulation of vascular niches for haematopoietic stem cells Nature, 2016-04-13;0(0):. 2016-04-13 [PMID: 27074508] (Flow Cytometry, Mouse)
• Kusumbe A, Ramasamy S, Adams R Coupling of angiogenesis and osteogenesis by a specific vessel subtype in bone. Nature, 2014-03-12;507(7492):323-8. 2014-03-12 [PMID: 24646994] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: PECAM1
• Uniprot:
  • Q08481()