Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) [NB600-1327] - Characterization of microglia cultures by multiple staining with anti CD11b (panels a. & c.) and anti GFAP (panels b & d). Nuclei appear ...read more
Immunohistochemistry: CD11b Antibody (M1/70.15) [NB600-1327] - Analysis of forzen lymph node section followed by staining with HRP secondary antibody.
Flow Cytometry: CD11b Antibody (M1/70.15) [NB600-1327] - Analysis using the Alexa Fluor (R) 488 conjugate of NB600-1327. Staining of mouse peritoneal macrophages using Alexa Fluor 488 conjugated CD11b antibody (clone ...read more
Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) [NB600-1327] - ICC/IF analysis of RAW264.7 cells using CD11b antibody (clone M1/70.15) with Dylight 488 conjugated secondary (green). Nuclei and tubulin ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Protein G purified
IHC-P: Perform enzymatic antigen retrieval before commencing with IHC staining protocol. NB600-1327 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue. May also be used on frozen sections. Immunocytochemistry (PMID: 21464229)
Read Publications using NB600-1327 in the following applications:
CD11Bintegrin, alpha M (complement component receptor 3, alpha; also known as CD11b(p170), macrophage antigen alpha polypeptide)
Cell surface glycoprotein MAC-1 subunit alpha
CR-3 alpha chain
Integrin alpha M
integrin, alpha M (complement component 3 receptor 3 subunit)
Leukocyte adhesion receptor MO1
M1/70.15 CD11b Antibody
M1/70.15 ICC Antibody
M1/70.15 IHC Antibody
M1/70.15 Immunocytochemistry Antibody
M1/70.15 Immunohistochemistry Antibody
M1/70.15 Microglia Marker
M1/70.15 Myeloid Marker
macrophage antigen alpha polypeptide
neutrophil adherence receptor alpha-M subunit
Neutrophil adherence receptor
Integrins are a family of heterodimeric transmembrane receptor proteins and they contains alpha as well as beta subunits. The Integrin alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin beta 2 subunit/CD18 to form the non-covalent heterodimer Integrin alpha M/beta 2, also known as MAC-1 and complement receptor type 3 /CR3. The Mac-1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes and is commonly used as a microglial marker in nervous tissue. CD11b is involved in adhesive interactions of monocytes, macrophages and granulocytes, and in mediating the uptake of complement coated particles. It has been shown to act as a receptor of iC3b, fibrinogen, factor-X, ICAM1, extracellular dsRNA, PAMP/DAMPs, aggregated beta-Amyloid and HMGB1. Variations at the ITGAM gene, which encodes for the CD11b chain of the Mac-1 integrin, is one of the main genetic risk factors involved in systemic lupus erythematosus /SLE disorder.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for CD11b Antibody (NB600-1327). (Showing 1 - 7 of 7 FAQs).
I am looking for a Mac-1 (microglia cell marker) antibody to do immunostaining with my mouse brain and I checked, you have a lot of antibodies for this biomarker. I don't know which one is the best to be used for immunohistology on mouse brain tissue. Could you please help with this?
I would recommend NB110-89474. This antibody has been published in 11 scientific articles, including several times in IHC in mouse. As long as CD11b is expressed in your sample, it should work just fine and is backed by our 100% Novus Guarantee.
What types of cells can this CD11b antibody be used as a marker for?
A study used this CD11b antibody to stain granulocytes in mouse uterine tissue in IHC. CD11b is also expressed on other leukocytes such as monocytes, macrophages, and natural killer cells.
Do you know if this CD11b antibody can be used in CD11b positive cell depletion?
This particular CD11b antibody is not reccomended for depletion studies due to the preservative used; however, our CD11b antibody NBP1-06650 is offered in a preservative free format and has been validated for blocking.
Looking for a bone marrow macrophage marker can you recommend a CD11b antibody for ICC?
I would recommend NB110-89474 which is our most popular CD11b antibody and has been validated in ICC and cited in 18 publications.
We need a CD11b antibody for IHC-P on mouse tissue conjugated to FITC, do you have one that will work?
We have 3 CD11b antibodies for IHC-P and just one CD11 b antibody we offer conjugated to FITC, NB110-89474F.
The protocol says to do enzymatic antigen retrieval before incubating with the CD11b antibody, which enzyme is used?
The protocol is referencting the PIER method using Proteinase K as the enzyme.
I'm not sure if this will work for me, can I get a free sample of your CD11b antibody to test?
We don't offer free samples but we do offer this CD11b antibody in a trial size of 0.025 ml at a reduced cost, all antibodies are backed by our 100% guarantee to work in all validated species and applications on the product's web page.
CD11b - More than a microglial marker The protein CD11b has been implicated in the various adhesion-related interactions of cells such as monocytes, macrophages, natural killer (NK) cells, and granulocytes. It is part of a heterodimer that consists of CD11b and CD18. It also modulates the... Read full blog post.