CD11b Antibody (M1/70.15)

Images

 
Immunohistochemistry: CD11b Antibody (M1/70.15) [NB600-1327] - Analysis of forzen lymph node section followed by staining with HRP secondary antibody.
Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) [NB600-1327] - Characterization of microglia cultures by multiple staining with anti CD11b (panels a. & c.) and anti GFAP (panels b & d). Nuclei appear ...read more
Flow Cytometry: CD11b Antibody (M1/70.15) [NB600-1327] - Analysis using the Alexa Fluor (R) 488 conjugate of NB600-1327. Staining of mouse peritoneal macrophages using Alexa Fluor 488 conjugated CD11b antibody (clone ...read more
Flow Cytometry: CD11b Antibody (M1/70.15) [NB600-1327] - Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b:RPE
Flow Cytometry: CD11b Antibody (M1/70.15) [NB600-1327] - Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b
Flow Cytometry: CD11b Antibody (M1/70.15) [NB600-1327] - Staining of total mouse peritoneal exudate cells demonstrating labelling of macrophages with Rat anti Mouse CD11b:RPE
Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) [NB600-1327] - ICC/IF analysis of RAW264.7 cells using CD11b antibody (clone M1/70.15) with Dylight 488 conjugated secondary (green). Nuclei and tubulin ...read more
Flow Cytometry: CD11b Antibody (M1/70.15) [NB600-1327] - Staining of mouse peritoneal macrophages cells.
Flow Cytometry: CD11b Antibody (M1/70.15) [NB600-1327] - Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b: RPE - Alexa Fluor 750
Flow Cytometry: CD11b Antibody (M1/70.15) [NB600-1327] - Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b

Product Details

Summary
Reactivity Hu, Mu, RbSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready
Clone
M1/70.15
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

CD11b Antibody (M1/70.15) Summary

Immunogen
T cell enriched splenocytes from B10 mice
Marker
Microglia Marker, Myeloid Marker
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
ITGAM
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • CyTOF-ready
Application Notes
IHC-P: Perform enzymatic antigen retrieval before commencing with IHC staining protocol. NB600-1327 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue. May also be used on frozen sections. Immunocytochemistry (PMID: 21464229)
Theoretical MW
127 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB600-1327 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CD11b Antibody (M1/70.15)

  • antigen CD11b (p170)
  • CD11 antigen-like family member B
  • CD11b antigen
  • cd11b flow antibody
  • CD11b
  • CD11Bintegrin, alpha M (complement component receptor 3, alpha; also known as CD11b(p170), macrophage antigen alpha polypeptide)
  • Cell surface glycoprotein MAC-1 subunit alpha
  • CR-3 alpha chain
  • CR3AMGC117044
  • Integrin alpha M
  • integrin alpha-M
  • integrin, alpha M (complement component 3 receptor 3 subunit)
  • ITGAM
  • Leukocyte adhesion receptor MO1
  • M1/70 cd11b
  • m1/70 clone
  • M1/70
  • M1/70.15 Antibody
  • M1/70.15 CD11b Antibody
  • M1/70.15 CD11b
  • M1/70.15 Clone
  • M1/70.15 Conjugated
  • M1/70.15 ICC Antibody
  • M1/70.15 IHC Antibody
  • M1/70.15 Immunocytochemistry Antibody
  • M1/70.15 Immunohistochemistry Antibody
  • M1/70.15 Microglia Marker
  • M1/70.15 Myeloid Marker
  • MAC-1
  • MAC1A
  • macrophage antigen alpha polypeptide
  • MO1A
  • neutrophil adherence receptor alpha-M subunit
  • Neutrophil adherence receptor
  • SLEB6

Background

Integrins are a family of heterodimeric transmembrane receptor proteins and they contains alpha as well as beta subunits. The Integrin alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin beta 2 subunit/CD18 to form the non-covalent heterodimer Integrin alpha M/beta 2, also known as MAC-1 and complement receptor type 3 /CR3. The Mac-1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes and is commonly used as a microglial marker in nervous tissue. CD11b is involved in adhesive interactions of monocytes, macrophages and granulocytes, and in mediating the uptake of complement coated particles. It has been shown to act as a receptor of iC3b, fibrinogen, factor-X, ICAM1, extracellular dsRNA, PAMP/DAMPs, aggregated beta-Amyloid and HMGB1. Variations at the ITGAM gene, which encodes for the CD11b chain of the Mac-1 integrin, is one of the main genetic risk factors involved in systemic lupus erythematosus /SLE disorder.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB600-1327
Species: Hu, Mu, Rb
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready

Publications for CD11b Antibody (NB600-1327)(10)

We have publications tested in 3 confirmed species: Human, Mouse, Rabbit.

We have publications tested in 4 applications: FLOW, Flow, IHC-Fr, IHC-P.


Filter By Application
FLOW
(1)
Flow
(1)
IHC-Fr
(1)
IHC-P
(2)
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Human
(1)
Mouse
(1)
Rabbit
(2)
All Species
Showing Publications 1 - 10 of 10.
Publications using NB600-1327 Applications Species
Gallego-Munoz P, Ibares-Frias L, Lorenzo E et al. Corneal Wound Repair After Rose Bengal and Green Light Crosslinking: Clinical and Histologic Study Invest. Ophthalmol. Vis. Sci. 2017 Jul 01 [PMID: 28700779] (IHC-P, Rabbit) IHC-P Rabbit
Amos PJ, Fung S, Case A et al. Modulation of Hematopoietic Lineage Specification Impacts TREM2 Expression in Microglia-Like Cells Derived From Human Stem Cells ASN Neuro 2017 Jul 07 [PMID: 28683563] (Flow, Human) Flow Human
Kim JY, Joo SW, Sunwoo JH et al. Lower Energy to Make a Corneal Flap with a 60 kHz Femtosecond Laser Reduces Flap Inflammation and Corneal Stromal Cell Death But Weakens Flap Adhesion Korean J Ophthalmol 2013 Apr [PMID: 23543236] (IHC-P, Rabbit) IHC-P Rabbit
McLellan AD, Kapp M, Eggert A et al. Anatomic location and T-cell stimulatory functions of mouse dendritic cell subsets defined by CD4 and CD8 expression. Blood. [PMID: 11877283] (FLOW, Mouse) FLOW Mouse
Welt, FG et al. Neutrophil, not macrophage, infiltration precedes neointimal thickening in balloon-injured arteries. Arterioscler Thromb Vasc Biol 20: 2553-2558. 2000 [PMID: 11116052]
Whiteland, JL et al. Immunohistochemical detection of T cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J Histochem Cytochem 43:313-320. 1995 [PMID: 7868861]
Beller, DI et al. Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor J. J. Exp. Med. 156:1000-1009. 1982 [PMID: 7153706]
Springer, T et al. (1979) Mac-1: a macrophage differentiation antigen identified by a monoclonal antibody. Eur J Immunol 9:301-306. 1979 [PMID: 89034]
Springer, T et al. (1978) Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. 8:539-551. 1978 [PMID: 81133]
Batazzi A. Elucidating the role of Keratin 17 in cervical carcinogenesis Thesis. 2014 (IHC-Fr)

Details:
Integrin alpha M/CD11b antibody used in IHC-Fr application for the detection of macrophages and neutrophils on un-fixed cervical epithelium cryosections from WT, HPV16tg/+K17-/- and HPV16tg/+K17+/+ mice. The data showed that HPV16tg/+K17-/- mice have a higher number of intra-epithelial immune cells (Figure 14, page 39)
IHC-Fr

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Product General Protocols

Video Protocols

ICC/IF Video Protocol

FAQs for CD11b Antibody (NB600-1327). (Showing 1 - 7 of 7 FAQs).

  1. I am looking for a Mac-1 (microglia cell marker) antibody to do immunostaining with my mouse brain and I checked, you have a lot of antibodies for this biomarker. I don't know which one is the best to be used for immunohistology on mouse brain tissue. Could you please help with this?
    • I would recommend NB110-89474. This antibody has been published in 11 scientific articles, including several times in IHC in mouse. As long as CD11b is expressed in your sample, it should work just fine and is backed by our 100% Novus Guarantee.
  2. What types of cells can this CD11b antibody be used as a marker for?
    • A study used this CD11b antibody to stain granulocytes in mouse uterine tissue in IHC. CD11b is also expressed on other leukocytes such as monocytes, macrophages, and natural killer cells.
  3. Do you know if this CD11b antibody can be used in CD11b positive cell depletion? 
    • This particular CD11b antibody is not reccomended for depletion studies due to the preservative used; however, our CD11b antibody NBP1-06650 is offered in a preservative free format and has been validated for blocking.
  4. Looking for a bone marrow macrophage marker can you recommend a CD11b antibody for ICC?
    • I would recommend NB110-89474 which is our most popular CD11b antibody and has been validated in ICC and cited in 18 publications.
  5. We need a CD11b antibody for IHC-P on mouse tissue conjugated to FITC, do you have one that will work?
    • We have 3 CD11b antibodies for IHC-P and just one CD11 b antibody we offer conjugated to FITC, NB110-89474F.
  6. The protocol says to do enzymatic antigen retrieval before incubating with the CD11b antibody, which enzyme is used?
    • The protocol is referencting the PIER method using Proteinase K as the enzyme.
  7. I'm not sure if this will work for me, can I get a free sample of your CD11b antibody to test?
    • We don't offer free samples but we do offer this CD11b antibody in a trial size of 0.025 ml at a reduced cost, all antibodies are backed by our 100% guarantee to work in all validated species and applications on the product's web page.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB600-1327AF405
Alexa Fluor 488 Labeled NB600-1327AF488
Alexa Fluor 647 Labeled NB600-1327AF647
Alexa Fluor 700 Labeled NB600-1327AF700
Allophycocyanin Labeled NB600-1327APC
Biotin Labeled NB600-1327B
DyLight 350 Labeled NB600-1327UV
DyLight 405 Labeled NB600-1327V
DyLight 488 Labeled NB600-1327G
DyLight 550 Labeled NB600-1327R
DyLight 650 Labeled NB600-1327C
DyLight 680 Labeled NB600-1327FR
DyLight 755 Labeled NB600-1327IR
HRP Labeled NB600-1327H
PE Labeled NB600-1327PE
PerCP Labeled NB600-1327PCP

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Blogs on CD11b.

Topics in CD11b: The innate immune response
Integrins are transmembrane receptors composed of alpha and beta chains, where beta-integrins are mainly expressed in leukocytes. Leukocytes are white blood cells that act in the immune system to defend our body against foreign pathogens.  Integrin...  Read full blog post.

CD11b - More than a microglial marker
The protein CD11b has been implicated in the various adhesion-related interactions of cells such as monocytes, macrophages, natural killer (NK) cells, and granulocytes. It is part of a heterodimer that consists of CD11b and CD18. It also modulates the...  Read full blog post.

CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.

Adhesion Receptor Molecule CD11b/c is the Point of Entry for many Infectious Diseases
Pathogenic microorganisms utilize a variety of cell surface receptors to gain entry into host cells and to bypass the natural defense mechanisms. One of the most prominent receptors used in this fashion is the leukocyte adhesion receptor CD11b/c. This...  Read full blog post.

CD11b/CD18 and Neutrophil-Epithelial Interactions as Targets for Anti-Inflammatory Therapies
The beta-2 integrins, a family of four cell surface transmembrane glycoproteins expressed only on leukocytes, include CD11a/CD18, CD11b/CD18, CD11c/CD18, and CD11d/CD18. They consist of a common beta subunit (CD18) and homologous alpha subunits (CD11a...  Read full blog post.

The CD11b Antibody: A Marker for Microglial Cells
Microglia are the resident macrophages of the central nervous system, and the first line of immune defense. Pioneering antibody research in the 1990's identified the Integrin beta 2 protein (also called ITGB2, complement receptor 3, CR3, CD18, and Mac...  Read full blog post.

CD11b Expression, Leukocyte Adhesion and the Innate Immune System
CD11b is an integrin family member which pairs with CD18 to form the CR3 heterodimer. CD11b is expressed on the surface of many leukocytes including monocytes, neutrophils, natural killer cells, granulocytes and macrophages, as well as on 8% of spleen...  Read full blog post.

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Bioinformatics

Gene Symbol ITGAM
Entrez
Uniprot