CD11b Antibody (M1/70.15)

Images

 
Immunohistochemistry: CD11b Antibody (M1/70.15) [NB600-1327] - Immunohistochemical analysis of frozen lymph node section using CD11b Antibody (M1/70.15) [NB600-1327] followed by staining with HRP-conjugated secondary ...read more
Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) [NB600-1327] - Characterization of microglia cultures by multiple staining with CD11b Antibody (M1/70.15) [NB600-1327] (panels a. & c.) and anti GFAP ...read more
Flow Cytometry: CD11b Antibody (M1/70.15) [NB600-1327] - A surface stain was performed on Raw264.7 cells with CD11b (M1/70.15) Antibody [NB600-1327R] (blue) and a matched isotype control (orange), both conjugated to ...read more
Biological Strategies: Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) [NB600-1327] - Immunocytochemical/Immunofluorescent analysis in mouse primary microglia. Cells were treated with ...read more
Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) [NB600-1327] - Immunocytochemistry/ Immunofluorescence (ICC/IF) analysis of RAW264.7 cells using CD11b antibody (M1/70.15) [NB600-1327] with Dylight 488 ...read more
Immunocytochemistry/ Immunofluorescence: CD11b Antibody (M1/70.15) [NB600-1327] - Immunocytochemical/Immunofluorescent staining in untreated and treated mouse microglia cells using CD11b Antibody (M1/70.15) ...read more
Flow Cytometry: CD11b Antibody (M1/70.15) [NB600-1327] - Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b Antibody (M1/70.15) [NB600-1327].
Flow Cytometry: CD11b Antibody (M1/70.15) [NB600-1327] - Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b Antibody (M1/70.15) [NB600-1327]: RPE - Alexa Fluor 750
Flow Cytometry: CD11b Antibody (M1/70.15) [NB600-1327] - Staining of mouse peritoneal macrophages using the Alexa Fluor (R) 488 conjugate of CD11b Antibody (M1/70.15) [NB600-1327AF488].

Product Details

Summary
Reactivity Hu, Mu, RbSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready
Clone
M1/70.15
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

CD11b Antibody (M1/70.15) Summary

Immunogen
Rat Monoclonal CD11b Antibody (M1/70.15) was made to T cell enriched splenocytes from B10 mice.
Marker
Microglia Marker, Myeloid Marker
Isotype
IgG2b Lambda
Clonality
Monoclonal
Host
Rat
Gene
ITGAM
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • CyTOF-ready
Application Notes
IHC-P: Perform enzymatic antigen retrieval before commencing with IHC staining protocol. NB600-1327 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue. May also be used on frozen sections. This antibody is CyTOF ready. ICC/IF reported in a verified customer review. Use in flow cytometry reported in scientific literature (PMID: 28683563). Use in Immunohistochemistry-Frozen sections reported in scientific literature (refer to Reviews & Publications). Use in Immunohistochemistry-Paraffin embedded sections reported in scientific literature (PMID: 28700779).
Theoretical MW
127.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 5
using
NB600-1327 in the following applications:

Publications
Read Publications using
NB600-1327 in the following applications:

Reactivity Notes

Mouse reactivity reported in a verified customer review. Rabbit reactivity reported in scientific literature (PMID: 28700779). Human reactivity reported in scientific literature (PMID: 28683563).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CD11b Antibody (M1/70.15)

  • antigen CD11b (p170)
  • CD11 antigen-like family member B
  • CD11b antigen
  • cd11b flow antibody
  • CD11b
  • Cell surface glycoprotein MAC-1 subunit alpha
  • Complement component 3 receptor 3 subunit
  • CR-3 alpha chain
  • CR3A
  • CR3AMGC117044
  • Integrin alpha M
  • integrin alpha-M
  • integrin, alpha M (complement component 3 receptor 3 subunit)
  • ITGAM
  • Leukocyte adhesion receptor MO1
  • M1/70 cd11b
  • m1/70 clone
  • M1/70
  • M1/70.15 Antibody
  • M1/70.15 CD11b Antibody
  • M1/70.15 CD11b
  • M1/70.15 Clone
  • M1/70.15 Conjugated
  • M1/70.15 ICC Antibody
  • M1/70.15 IHC Antibody
  • M1/70.15 Immunocytochemistry Antibody
  • M1/70.15 Immunohistochemistry Antibody
  • M1/70.15 Microglia Marker
  • M1/70.15 Myeloid Marker
  • MAC-1
  • MAC1A
  • macrophage antigen alpha polypeptide
  • MO1A
  • neutrophil adherence receptor alpha-M subunit
  • Neutrophil adherence receptor
  • SLEB6

Background

CD11b (integrin alpha M subunit) is a type I transmembrane glycoprotein. CD11b combines with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin alpha M/beta 2, also known as Mac-1 and complement receptor type 3 (CR3). The CD11b/CD18 (Mac-1) heterodimer has a theoretical molecular weight of 127kDa but is often observed near 170kDa due to post translational modifications. Integrins are transmembrane proteins that mediate interactions between adhesion molecules on adjacent cells and/or the extracellular matrix. Integrins have diverse roles in several biological processes, including cell migration during development, wound healing, cell differentiation, and apoptosis. The particular integrin CD11b is known as a pro-inflammatory molecule given its ability to promote phagocyte cytotoxic functions while enhancing several effector molecules such as FcGR, uPAR, and CD14 (1). In macrophages, CpG-DNA stimulates lysosomal degradation and vacuolar acidification, subsequently promoting CD11b release via TLR9 (2). During an acute infection, high CD11b/Mac-1 expression on antigen-specific CD8 (+) T cells can signify recent activation of the immune system, whereas naive T cells and virus-specific memory CD8 (+) T cells express little or no Mac-1 when exposed to a virus (3). Variations at the ITGAM gene, which encodes for the CD11b chain of the Mac-1 integrin, is one of the main genetic risk factors involved in systemic lupus erythematosus /SLE disorder (4).

References

1. Rosetti, F., & Mayadas, T. N. (2016). The many faces of Mac-1 in autoimmune disease. Immunol Rev, 269(1), 175-193. doi:10.1111/imr.12373

2. Kim, D., Kim, T. H., Wu, G., Park, B. K., Ha, J. H., Kim, Y. S.,... Kwon, H. J. (2016). Extracellular Release of CD11b by TLR9 Stimulation in Macrophages. PLoS One, 11(3), e0150677. doi:10.1371/journal.pone.0150677

3. Christensen, J. E., Andreasen, S. O., Christensen, J. P., & Thomsen, A. R. (2001). CD11b expression as a marker to distinguish between recently activated effector CD8(+) T cells and memory cells. Int Immunol, 13(4), 593-600. doi:10.1093/intimm/13.4.593

4. Nath, S. K., Han, S., Kim-Howard, X., Kelly, J. A., Viswanathan, P., Gilkeson, G. S.,... Harley, J. B. (2008). A nonsynonymous functional variant in integrin-alpha(M) (encoded by ITGAM) is associated with systemic lupus erythematosus. Nat Genet, 40(2), 152-154. doi:10.1038/ng.71

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready

Publications for CD11b Antibody (NB600-1327)(13)

We have publications tested in 3 confirmed species: Human, Mouse, Rabbit.

We have publications tested in 6 applications: FLOW, Flow, ICC/IF, IHC, IHC-Fr, IHC-P.


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FLOW
(1)
Flow
(1)
ICC/IF
(1)
IHC
(1)
IHC-Fr
(1)
IHC-P
(2)
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Human
(1)
Mouse
(3)
Rabbit
(2)
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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB600-1327 Applications Species
Gallego-Munoz P, Ibares-Frias L, Lorenzo E et al. Corneal Wound Repair After Rose Bengal and Green Light Crosslinking: Clinical and Histologic Study Invest. Ophthalmol. Vis. Sci. 2017 Jul 01 [PMID: 28700779] (IHC-P, Rabbit) IHC-P Rabbit
Amos PJ, Fung S, Case A et al. Modulation of Hematopoietic Lineage Specification Impacts TREM2 Expression in Microglia-Like Cells Derived From Human Stem Cells ASN Neuro 2017 Jul 07 [PMID: 28683563] (Flow, Human) Flow Human
Kim JY, Joo SW, Sunwoo JH et al. Lower Energy to Make a Corneal Flap with a 60 kHz Femtosecond Laser Reduces Flap Inflammation and Corneal Stromal Cell Death But Weakens Flap Adhesion Korean J Ophthalmol 2013 Apr [PMID: 23543236] (IHC-P, Rabbit) IHC-P Rabbit
Batazzi A. Elucidating the role of Keratin 17 in cervical carcinogenesis Thesis. 2014 (IHC-Fr)

Details:
Integrin alpha M/CD11b antibody used in IHC-Fr application for the detection of macrophages and neutrophils on un-fixed cervical epithelium cryosections from WT, HPV16tg/+K17-/- and HPV16tg/+K17+/+ mice. The data showed that HPV16tg/+K17-/- mice have a higher number of intra-epithelial immune cells (Figure 14, page 39)
IHC-Fr
Marini C, Cossu V, Bonifacino T et al. Mechanisms underlying the predictive power of high skeletal muscle uptake of FDG in amyotrophic lateral sclerosis EJNMMI Res Jul 7 2020 [PMID: 32638178] (IHC, Mouse) IHC Mouse
Plastira I, Bernhart E, Goeritzer M, et al. Lysophosphatidic acid via LPA-receptor 5/protein kinase D-dependent pathways induces a motile and pro-inflammatory microglial phenotype. J Neuroinflammation. 2016 Aug 26 [PMID: 29258556]
Plastira I, Bernhart E, Goeritzer M, et al. 1-Oleyl-lysophosphatidic acid (LPA) promotes polarization of BV-2 and primary murine microglia towards an M1-like phenotype. J Neuroinflammation. 2016 Aug 26 [PMID: 27565558] (ICC/IF, Mouse) ICC/IF Mouse
McLellan AD, Kapp M, Eggert A et al. Anatomic location and T-cell stimulatory functions of mouse dendritic cell subsets defined by CD4 and CD8 expression. Blood. [PMID: 11877283] (FLOW, Mouse) FLOW Mouse
Welt, FG et al. Neutrophil, not macrophage, infiltration precedes neointimal thickening in balloon-injured arteries. Arterioscler Thromb Vasc Biol 20: 2553-2558. 2000 [PMID: 11116052]
Whiteland, JL et al. Immunohistochemical detection of T cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J Histochem Cytochem 43:313-320. 1995 [PMID: 7868861]
Show All 13 Publications.

Reviews for CD11b Antibody (NB600-1327) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using NB600-1327:
Filter by Applications
ICC
(2)
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Mouse
(2)
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Images Ratings Applications Species Date Details
Immunocytochemistry CD11b NB600-1327
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reviewed by:
Lisha Joshi
ICC Mouse 06/10/2019
View

Summary

ApplicationImmunocytochemistry
Sample TestedMurine primary microglia
SpeciesMouse
Lot0113

Comments

CommentsCells were treated with LPA or LPA and an LPAR5 inhibitor in order to investigate the expression of iNOS after 24 hours. CD11b antibody was used in a dilution 1:100 in order to stain primary murine microglia.Cy2 (green) and Cy3 (red) were used as secondary antibodies.
Immunocytochemistry CD11b NB600-1327
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reviewed by:
Joanna Plastira
ICC Mouse 06/07/2019
View

Summary

ApplicationImmunocytochemistry
Sample TestedMicroglia cells
SpeciesMouse
Lot0113

Product General Protocols

Video Protocols

ICC/IF Video Protocol

FAQs for CD11b Antibody (NB600-1327). (Showing 1 - 8 of 8 FAQs).

  1. I am looking for a Mac-1 (microglia cell marker) antibody to do immunostaining with my mouse brain and I checked, you have a lot of antibodies for this biomarker. I don't know which one is the best to be used for immunohistology on mouse brain tissue. Could you please help with this?
    • I would recommend NB110-89474. This antibody has been published in 11 scientific articles, including several times in IHC in mouse. As long as CD11b is expressed in your sample, it should work just fine and is backed by our 100% Novus Guarantee.
  2. What types of cells can this CD11b antibody be used as a marker for?
    • A study used this CD11b antibody to stain granulocytes in mouse uterine tissue in IHC. CD11b is also expressed on other leukocytes such as monocytes, macrophages, and natural killer cells.
  3. Do you know if this CD11b antibody can be used in CD11b positive cell depletion? 
    • This particular CD11b antibody is not reccomended for depletion studies due to the preservative used; however, our CD11b antibody NBP1-06650 is offered in a preservative free format and has been validated for blocking.
  4. Looking for a bone marrow macrophage marker can you recommend a CD11b antibody for ICC?
    • I would recommend NB110-89474 which is our most popular CD11b antibody and has been validated in ICC and cited in 18 publications.
  5. We need a CD11b antibody for IHC-P on mouse tissue conjugated to FITC, do you have one that will work?
    • We have 3 CD11b antibodies for IHC-P and just one CD11 b antibody we offer conjugated to FITC, NB110-89474F.
  6. The protocol says to do enzymatic antigen retrieval before incubating with the CD11b antibody, which enzyme is used?
    • The protocol is referencting the PIER method using Proteinase K as the enzyme.
  7. I'm not sure if this will work for me, can I get a free sample of your CD11b antibody to test?
    • We don't offer free samples but we do offer this CD11b antibody in a trial size of 0.025 ml at a reduced cost, all antibodies are backed by our 100% guarantee to work in all validated species and applications on the product's web page.
  8. Is CD11b antibody also staining neutrophils in the brain
    • CD11b is typically used as a marker for granulocytes and macrophages. You may need a combination of markers to specifically identify neutrophils

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB600-1327AF350
Alexa Fluor 405 NB600-1327AF405
Alexa Fluor 488 NB600-1327AF488
Alexa Fluor 532 NB600-1327AF532
Alexa Fluor 594 NB600-1327AF594
Alexa Fluor 647 NB600-1327AF647
Alexa Fluor 700 NB600-1327AF700
Alexa Fluor 750 NB600-1327AF750
Allophycocyanin NB600-1327APC
Allophycocyanin/Cy7 NB600-1327APCCY7
Biotin NB600-1327B
DyLight 350 NB600-1327UV
DyLight 405 NB600-1327V
DyLight 488 NB600-1327G
DyLight 550 NB600-1327R
DyLight 594 NB600-1327DL594
DyLight 650 NB600-1327C
DyLight 680 NB600-1327FR
DyLight 755 NB600-1327IR
HRP NB600-1327H
Janelia Fluor 549 NB600-1327JF549
Janelia Fluor 646 NB600-1327JF646
PE NB600-1327PE
PE/Atto594 NB600-1327PEATT594
PE/Cy5.5 NB600-1327PECY55
PE/Cy7 NB600-1327PECY7
PerCP NB600-1327PCP

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Blogs on CD11b.

Gut-brain axis: microbiota influence behavior and mental well-being
By Jennifer Sokolowski, MD, PhD.Gut-microbiome interactionsOn and in our bodies, microbes outnumber our cells by about ten-to-one. Studies have revealed that the microbiome influences neurogenesis, cognition, and...  Read full blog post.

Antibody treatment can generate microglia-like cells from bone marrow
By Jennifer Sokolowski, MD, PhD.Microglia play important roles in the brain in both homeostatic and pathological conditions, acting to clear debris and dying cells. There is evidence to suggest that microglial dys...  Read full blog post.

TMEM 119 is a specific marker of microglia cells
By Jennifer Sokolowski, MD, PhD.Microglia are a major immune-cell component in the brain. They ingest and degrade dead cells, debris, and foreign material and interact with other immune cells to orchestrate centra...  Read full blog post.

Topics in CD11b: The innate immune response
Integrins are transmembrane receptors composed of alpha and beta chains, where beta-integrins are mainly expressed in leukocytes. Leukocytes are white blood cells that act in the immune system to defend our body against foreign pathogens.  Integrin...  Read full blog post.

CD11b - More than a microglial marker
The protein CD11b has been implicated in the various adhesion-related interactions of cells such as monocytes, macrophages, natural killer (NK) cells, and granulocytes. It is part of a heterodimer that consists of CD11b and CD18. It also modulates the...  Read full blog post.

CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.

Adhesion Receptor Molecule CD11b/c is the Point of Entry for many Infectious Diseases
Pathogenic microorganisms utilize a variety of cell surface receptors to gain entry into host cells and to bypass the natural defense mechanisms. One of the most prominent receptors used in this fashion is the leukocyte adhesion receptor CD11b/c. This...  Read full blog post.

CD11b/CD18 and Neutrophil-Epithelial Interactions as Targets for Anti-Inflammatory Therapies
The beta-2 integrins, a family of four cell surface transmembrane glycoproteins expressed only on leukocytes, include CD11a/CD18, CD11b/CD18, CD11c/CD18, and CD11d/CD18. They consist of a common beta subunit (CD18) and homologous alpha subunits (CD11a...  Read full blog post.

The CD11b Antibody: A Marker for Microglial Cells
Microglia are the resident macrophages of the central nervous system, and the first line of immune defense. Pioneering antibody research in the 1990's identified the Integrin beta 2 protein (also called ITGB2, complement receptor 3, CR3, CD18, and Mac...  Read full blog post.

CD11b Expression, Leukocyte Adhesion and the Innate Immune System
CD11b is an integrin family member which pairs with CD18 to form the CR3 heterodimer. CD11b is expressed on the surface of many leukocytes including monocytes, neutrophils, natural killer cells, granulocytes and macrophages, as well as on 8% of spleen...  Read full blog post.

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
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Lisha Joshi
06/10/2019
Application: ICC
Species: Mouse

Joanna Plastira
06/07/2019
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol ITGAM
Entrez
Uniprot