CD117/c-kit Antibody [Biotin]

• Reactivity: Mu
• Applications: WB, Flow, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

CD117/c-kit Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CD117/c-kit
  Gln25-Thr519 (Ala207Glu)
  Accession # P05532
• Specificity: Detects mouse CD117/c-kit in Western blots. In Western blots, approximately 10% cross‑reactivity with recombinant human CD117 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KIT
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD117/c-kit Antibody [Biotin]

• CD117 antigen
• CD117
• ckit
• c-kit
• EC 2.7.10
• EC 2.7.10.1
• KIT
• PBT
• piebald trait
• Proto-oncogene c-Kit
• proto-oncogene tyrosine-protein kinase Kit
• SCF R
• SCFR
• SCFRmast/stem cell growth factor receptor
• soluble KIT variant 1
• Tyrosine-protein kinase Kit
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

Stem cell factor receptor (CD117, the gene product of the c-kit protooncogene) and its ligand, stem cell factor (also named c-kit ligand, mast cell growth factor), play essential roles in gametogenesis, melanogenesis and hematopoiesis. It is a transmembrane tyrosine kinase that is expressed on endothelial cells, mast cells, megakaryocytes, stem cells and multiple embryonic cells, such as melanoblasts and primordial germ cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD117/c-kit Antibody (BAF1356)(5)

Publications using BAF1356

• Higaki, K;Aiba, S;Shimoyama, T;Omatsu, Y;Nagasawa, T; Universal fibroblasts across tissues can differentiate into niche cells for hematopoietic stem cells Cell reports 2025-04-30 [PMID: 40315055] (Flow Cytometry, Mouse)
• Krepuska, M;Mayer, B;Vitale-Cross, L;Myneni, VD;Boyajian, MK;Németh, K;Szalayova, I;Cho, T;Mc Clain-Caldwell, I;Gingerich, AD;Han, H;Westerman, M;Rada, B;Mezey, É; Bone marrow stromal cell-derived hepcidin has antimicrobial and immunomodulatory activities Scientific reports 2024-02-17 [PMID: 38368463] (Immunocytochemistry, Mouse)
• T Chakrabort, MK Driscoll, E Jeffery, MM Murphy, P Roudot, BJ Chang, S Vora, WM Wong, CD Nielson, H Zhang, V Zhemkov, C Hiremath, ED De La Cruz, Y Yi, I Bezprozvan, H Zhao, R Tomer, R Heintzmann, JP Meeks, DK Marciano, SJ Morrison, G Danuser, KM Dean, R Fiolka Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution Nat. Methods, 2019-10-31;16(11):1109-1113. 2019-10-31 [PMID: 31673159] (ICC, Mouse)
• DL Coutu, KD Kokkaliari, L Kunz, T Schroeder Multicolor quantitative confocal imaging cytometry Nat. Methods, 2017-11-13;15(1):39-46. 2017-11-13 [PMID: 29320487] (IHC, Mouse)
• Melih Acar, Kiranmai S. Kocherlakota, Malea M. Murphy, James G. Peyer, Hideyuki Oguro, Christopher N. Inra et al. Deep imaging of bone marrow shows non-dividing stem cells are mainly perisinusoidal Nature 10/1/2015 [PMID: 26416744]

Bioinformatics

• Gene Symbol: KIT
• Uniprot:
  • P05532()