Flt-3/Flk-2/CD135 Antibody (7E8.2C8)

Images

 
Western Blot: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [NBP2-42210] - WB analysis of lysates from (A) Human Embryonic Kidney 293 / HEK293 and (B) Human Embryonic carcinoma NCCIT cell lines using Flt-3/Flk-2/CD135 antibody ...read more
Immunohistochemistry-Paraffin: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [NBP2-42210] - IHC analysis of a formalin fixed paraffin embedded tissue section of human liver cancer using Flt-3/Flk-2/CD135 antibody (clone 7E2.2C8) ...read more
Flow Cytometry: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [NBP2-42210] - HEK293 cells were stained (surface) with Flt-3/Flk-2/CD135 antibody (clone: 7E8.2C8; red) or isotype control (mouse IgG2 lambda; blue) and positive ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, IHC-P
Clone
7E8.2C8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Flt-3/Flk-2/CD135 Antibody (7E8.2C8) Summary

Immunogen
Partial recombinant human FLT3 protein (between amino acids 250-500) [UniProt P36888]
Localization
Endoplasmic reticulum lumen, Membrane
Isotype
IgG2b Lambda
Clonality
Monoclonal
Host
Mouse
Gene
FLT3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Flow Cytometry 2 ug/million cells
  • Immunohistochemistry 5-15 ug/ml
  • Immunohistochemistry-Paraffin 5-15 ug/ml
Application Notes
FLT3 is a 993 amino acids long protein with predicted molecular weight of 112.9 kDa, however, it undergoes extensive glycosylation and phosphorylation so that the mature protein will have relatively higher molecular weights. In WB assay, the glycosylated protein species may run between 113-160 kDa position, while the phosphorylated protein may result on generation of a doublet band.
Positive Control
Flt-3/Flk-2/CD135 Lysate (NBP2-11587)

Reactivity Notes

Immunogen has ~86% homology to Mouse and Rat's FLT3 protein.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Flt-3/Flk-2/CD135 Antibody (7E8.2C8)

  • CD135 antigen
  • CD135
  • EC 2.7.10
  • fetal liver kinase 2
  • FL cytokine receptor
  • FLK2
  • Flk-2
  • FLT3 receptor tyrosine kinase
  • Flt3
  • Flt-3
  • Fms-like tyrosine kinase 3
  • fms-related tyrosine kinase 3
  • growth factor receptor tyrosine kinase type III
  • Stem cell tyrosine kinase 1
  • STK-1
  • STK1EC 2.7.10.1
  • Tyrosine-protein kinase receptor FLT3

Background

CD135 is a tyrosine kinase receptor expressed on normal cells including CD34+ hematopoietic stem cells, myelomonocytic progenitors, primitive B cell progenitors, and thymocytes. CD135 is also expressed on malignant hematopoietic cells including AML, ALL and CML BC. CD135, also known as FMS-like tyrosine kinase 3, FLT3, STK1, and Flk2, is a growth factor receptor that binds the FLT3 ligand to promote the growth and differentiation of primitive hematopoietic cells. The intracytoplasmic domain of CD135 is modified by phosphorylation and has been shown to interact with Grb2, SOCS1, VAV1, and Shc. In humans, expression of Flt3 is restricted to subsets of CD34 positive as well as CD34 negative normal bone marrow cells. In these cells, the level of expression of Flt3 is rather low. Most of the CD34 bright Flt3+ cells co-express CD117 at high levels. They may represent early cycling, but not quiescent stem cells. Flt3+ cells in the CD34lo and CD34- populations do not co-express CD117 molecule and may represent B lymphoid precursors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-42210AF405
Alexa Fluor 488 Labeled NBP2-42210AF488
Alexa Fluor 647 Labeled NBP2-42210AF647
Alexa Fluor 700 Labeled NBP2-42210AF700
Allophycocyanin Labeled NBP2-42210APC
Biotin Labeled NBP2-42210B
DyLight 350 Labeled NBP2-42210UV
DyLight 405 Labeled NBP2-42210V
DyLight 488 Labeled NBP2-42210G
DyLight 550 Labeled NBP2-42210R
DyLight 650 Labeled NBP2-42210C
DyLight 680 Labeled NBP2-42210FR
DyLight 755 Labeled NBP2-42210IR
HRP Labeled NBP2-42210H
PE Labeled NBP2-42210PE
PerCP Labeled NBP2-42210PCP

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Bioinformatics

Gene Symbol FLT3
Entrez
OMIM
Uniprot