CCR7: Novus Biologicals

MAB197

CCR7 Antibody (150503) [Unconjugated]

(1 Review)

100 μg

MAB197

MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P) and either (A) Mouse Anti-Human CCR7 Monoclonal Antibody (Catalog # MAB197) or (B) Mouse IgG<sub>2A</sub>Isotype Control (Catalog # <a class=

MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." alt="Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P) and either (A) Mouse Anti-Human CCR7 Monoclonal Antibody (Catalog # MAB197) or (B) Mouse IgG_2AIsotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

CCR7 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 25 µg/mL Mouse Anti-Human CCR7 Monoclonal Antibody (Catalog # MAB197) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for <a class=NoLineLink href=

Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in macrophages. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

CCR7 was detected in immersion fixed paraffin-embedded sections of human lung using Mouse Anti-Human CCR7 Monoclonal Antibody (Catalog # MAB197) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in macrophages. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

361-MI) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL19/MIP-3 beta (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCR7 Monoclonal Antibody (Catalog # MAB197). The ND₅₀ is typically 1-5 µg/mL." class="big_lightbox">

Recombinant Human CCL19/MIP-3 beta (Catalog # <a class=

361-MI) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL19/MIP-3 beta (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCR7 Monoclonal Antibody (Catalog # MAB197). The ND₅₀ is typically 1-5 µg/mL." class="big_thumb" />

<b>Biological Strategies Validation. </b>CXCR4 ligand binding facilitates CXCR4/CCR7 hetero-oligomer formation.(A) CCR7 homo-oligomer formation in H9 cells after treatment with or without 1 μg/ml recombinant gp120 was examined by in situ PLA. The number of in situ PLA signals per cell was counted by using the Duolink Image Tool software. The result shown is a representative result from three independent experiments showing the mean number of the signals plotted on the vertical axis. *, p < 0.05 by Mann-Whitney’s U test. (B) CCR7 homo-oligomer formation in H9 cells after treatment with 100 ng/ml CXCL12 was examined, as described in (A). Quantification of CCR7 homo-oligomers after CXCL12 pretreatment in the presence or absence of 1 or 5 μg/ml AMD3100. The result shown is a representative result from five independent experiments. *, p < 0.05 by Mann-Whitney’s U test. (C) CXCR4/CCR7 or CXCR4/CCR1 hetero-oligomer formation in H9 cells by in situ PLA using the indicated combinations of antibodies. The z-stack images derived from sections covering 10 μm with a 0.5-μm step are shown. Scale bar represents 50 μm. (D) Quantification of the detected in situ PLA signals per cell was performed. The result shown is a representative result from three independent experiments. *, p < 0.05 by Mann-Whitney’s U test. (E) CXCR4/CCR7 hetero-oligomer formation in H9 cells after treatment with 1 μg/ml gp120 in a native form (N) or control heat-denatured form (D) was examined by the PLA using the indicated combinations of antibodies. (F) CXCR4/CCR7 hetero-oligomer formation was examined with 100 ng/ml CXCL12 as described in (E). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0117454), licensed under a CC-BY license. Not internally tested by R&D Systems.

Dynamics of CCR7 expression on CD1c+ mDC and pDC during primary SIVmac239 infection.(A) The dynamics of CCR7 expression on CD1c+ mDC; (B) The dynamics of CCR7 expression on pDC. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0029036), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>CCR7 homodimers are dissociated by the CCR7 TM4-derived peptide. (A) The levels of bioluminescence signals are presented for HEK293T cells transfected with the indicated combinations of CCR7-CGLuc, CCR7-NGLuc, CGLuc and NGLuc plasmids (1.5 μg each). A representative experiment from at least three independent experiments is shown. Data represent mean ± SD of triplicate samples. *p < 0.05 by one-way ANOVA. (B) The levels of bioluminescence signals are shown for cells transfected with combinations of CCR7-CGLuc and CCR7-NGLuc in the presence of the CCR7 TM4 peptide or the shuffled peptide (15 μg/ml). Relative luminescence was obtained by normalizing the values against untransfected cells. A representative experiment from at least three independent experiments is shown. Data represent mean ± SD of triplicate samples. *p < 0.05 by Student’s t test. (C) The levels of CCR7 homodimer in parental H9 cells were measured by in situ PLA. CCR7-CCR1 heterodimer formation detected by anti-CCR7 mAb-PLAPlus and anti-CCR1 mAb -PLAMinus (left), and CCR7 homodimer formation by anti-CCR7 mAb-PLAPlus and -PLAMinus (right). A representative experiment from at least three independent experiments is shown with the mean number of PLA signals plotted on the vertical axis. *p < 0.05 by Mann-Whitney’s U test. (D) CCR7 (top) or CCR1 (bottom) homodimer formation after treatment with the CCR7 TM4 peptide (right panel) or the shuffled peptide (left panel). The number of PLA signals per cell was counted using the Duolink Image Tool software. A representative experiment from three independent experiments is shown with the mean number of PLA signals plotted on the vertical axis. *p < 0.05 by Mann-Whitney’s U test; NS, not significant. (E) CCR7 expression levels were evaluated in the presence (red open histogram) or absence (blue open histogram) of the CCR7 TM4 peptide (15 μg/ml) by flow cytometry using anti-human CCR7 antibody or isotype control antibody (gray-filled histogram). MFI is indicated on th

Two-color strategy for identifying CD1c+ mDCs and pDCs.(A) PBMCs (R1) were first gated on Forward-scatter/side-scatter (FSC/SSC) scattergram. Then, CD1c+ mDCs (CD1c+CD14−CD20−) were selected in the R2 region and pDCs (CD123brightHLA-DR+) were selected in the R3 region. (B) The expressions of immunophenotypes, including CD4, CCR5, CD80, CD83, CD86, and CCR7 on DCs subsets were analyzed. Open histograms correspond to isotype controls and filled gray histograms correspond to specific mAbs staining. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0029036), licensed under a CC-BY license. Not internally tested by R&D Systems.

MAB197) at 15ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. " class="big_lightbox">

Formalin-fixed paraffin-embedded tissue sections of human tonsil were probed for CCR7 mRNA (ACD RNAScope Probe, catalog # 410728; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using mouse anti-human CCR7 monoclonal antibody (R&D Systems catalog # <a class=

MAB197) at 15ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. " class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB197U-100UG
Alexa Fluor 405	FAB197V-100UG
Alexa Fluor 488	FAB197G
Alexa Fluor 532	FAB197X
Alexa Fluor 594	FAB197T-100UG
Alexa Fluor 647	FAB197R-100UG
Alexa Fluor 700	FAB197N-100
Alexa Fluor 700	FAB197N-025
Alexa Fluor 750	FAB197S-100UG
Alexa Fluor Plus 405	FAB197AFP405-100UG
Alexa Fluor Plus 488	FAB197AFP488-100UG
Alexa Fluor Plus 555	FAB197AFP555-100UG
Alexa Fluor Plus 594	FAB197AFP594-100UG
Alexa Fluor Plus 647	FAB197AFP647-100UG
Alexa Fluor Plus 680	FAB197AFP680-100UG
Allophycocyanin	FAB197A-025
Allophycocyanin	FAB197A-100
Allophycocyanin/Cy7	FAB197APCCY7
Biotin	FAB197B-100UG
CoraFluor 1	FAB197CL1
DyLight 350	FAB197D
DyLight 405	FAB197E
DyLight 488	FAB197K
DyLight 550	FAB197L
DyLight 594	FAB197M
DyLight 650	FAB197W
DyLight 680	FAB197Y
DyLight 755	FAB197Z
Fluorescein	FAB197F-025
Fluorescein	FAB197F-100
HRP	FAB197H
Janelia Fluor 525	FAB197JF525
Janelia Fluor 549	FAB197I
Janelia Fluor 646	FAB197J
Janelia Fluor 669	FAB197JF669
PE/Atto594	FAB197PEATT594
PE/Cy5.5	FAB197PECY55
PE/Cy7	FAB197PECY7
Phycoerythrin	FAB197P-100
Phycoerythrin	FAB197P-025
mFluor Violet 450 SE	FAB197MFV450
mFluor Violet 500 SE	FAB197MFV500
mFluor Violet 610 SE	FAB197MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB197T-100UG
Carrier Free	FAB197G
Carrier Free	FAB197S-100UG
Carrier Free	FAB197U-100UG
Carrier Free	FAB197V-100UG
Carrier Free	MAB197-SP
Carrier Free	FAB197N-100
Carrier Free	FAB197N-025
Carrier Free	FAB197A-100
Carrier Free	FAB197R-100UG
Carrier Free	FAB197A-025
Carrier Free	FAB197P-100
Carrier Free	FAB197P-025
Carrier Free	FAB197F-100
Carrier Free	FAB197F-025
Carrier Free	FAB197B-100UG

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NB100-712

CCR7 Antibody

(43 Publications)

0.02 mg

NB100-712

Immunohistochemistry-Paraffin: CCR7 Antibody [NB100-712] - Human spleen

Immunohistochemistry: CCR7 Antibody [NB100-712] - M1 expression pattern changed in trabecular bone. Immunohistochemical staining was performed in sagittal serial sections of medial condyles from 6-week-old rats within the epiphyseal region. The region with the solid line indicates areas of interest to be enlarged in the following image. Representative staining with the anticluster of differentiation 68 (CD68) antibody demonstrated that CD68+ cells were either attached (black arrows) or unattached (white arrows) to the bone surfaces with a variety of sizes & shapes (a, b). TRAP+ cells showed multinucleated morphology (black arrows) which is similar to the CD68+ cells (c, d). Inducible nitric oxide synthase-positive (iNOS+) & C-C chemokine receptor type 7-positive (CCR7+) cells (black arrow) were attached to the bone surfaces with an elongated morphology (e, f, i, j). Arginase1+ & cluster of differentiation 163-positive (CD163+) cells (white arrow) represented the predominant population in the reticular connective tissue with similar expression patterns as in the cortical bone (g, h, k, l). No positive staining was found in isotype controls (data not shown). Most of the M1-labeled cells were found attached to bone surfaces instead of M2-labeled cells (m). Representative images from three independent experiments are shown. FOV, field of view. Data shown as the mean±s.d. (*P<0.05). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29263936), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immune response of macrophages after blocking Piezo1. (A) IF staining of CCR7 and CD206 in the Control, LPS and GsMTx4+LPS groups after 4-day-cultured. M1 macrophages were marked with CCR7 (red), M2 macrophages with CD206 (green), and nuclei with DAPI (blue). (B) Semi-quantitative analysis of CCR7 and CD163 in each group. (C) Flow cytometry analysis of RAW264. 7 cells in the Control, LPS and GsMTx4+LPS groups. Q6 represents M1 types (F4/80+/iNOS+) and Q10 represents M2 types (F4/80+/CD206+). (D) Expressions of Piezo1 and inflammation-related genes (Tnfa and Il1b) in macrophages cultured for 4 days. (E) Western blotting analysis of PIEZO1, IL-1B and TNF-A in RAW264. 7 cultured for 4 days. (F) Intracellular ROS levels of RAW264. 7 cultured for 4 days. (G) Concentration of inflammatory cytokines in macrophage medium detected by ELISA. *P < 0. 05; **P < 0. 01; ***P < 0. 001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37261355), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immune response of macrophages after activating Piezo1. (A) IF staining of CCR7 and CD206 in the Control, LPS and Yoda1+LPS groups after 4-day-cultured. M1 macrophages were marked with CCR7 (red), M2 macrophages with CD206 (green), and nuclei with DAPI (blue). (B) Semi-quantitative analysis of CCR7 and CD206. (C) Flow cytometry analysis of RAW264. 7 cells in the Control, LPS and Yoda1+LPS groups. Q6 represents M1 types (F4/80+/iNOS+) and Q10 represents M2 types (F4/80+/CD206+). (D)Piezo1, Tnfa and Il1b genes expressions in macrophages cultured for 4 days. (E) Western blotting analysis of PIEZO1, TNF-A and IL-1B in RAW264. 7 cultured for 4 days. (F) Intracellular ROS levels of RAW264. 7 cultured for 4 days. (G) Concentration of inflammatory cytokines in macrophages medium detected by ELISA. *P < 0. 05; **P < 0. 01; ***P < 0. 001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37261355), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , ,

Applications: , , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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MAB3477

CCR7 Antibody (4B12) [Unconjugated]

(21 Publications)

100 μg

MAB3477

Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

Fluorescent ICC Staining of Non-adherent Cells." alt="CCR7 was detected in immersion fixed non-stimulated mouse splenocytes using 25 µg/mL Rat Anti-Mouse CCR7 Monoclonal Antibody (Catalog # MAB3477) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

440-M3) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL19/MIP-3 beta (25 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCR7 Monoclonal Antibody (Catalog # MAB3477). The ND₅₀ is typically 2-10 µg/mL." class="big_lightbox">

Recombinant Mouse CCL19/MIP-3 beta (Catalog # <a class=

440-M3) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL19/MIP-3 beta (25 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCR7 Monoclonal Antibody (Catalog # MAB3477). The ND₅₀ is typically 2-10 µg/mL." class="big_thumb" />

FAB4841P) and either (A) Rat Anti-Mouse CCR7 Monoclonal Antibody (Catalog # MAB3477) or (B) Rat IgG_2AIsotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # <a class=

FAB4841P) and either (A) Rat Anti-Mouse CCR7 Monoclonal Antibody (Catalog # MAB3477) or (B) Rat IgG_2AIsotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , ,

Applications: , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB3477U-100UG
Alexa Fluor 405	FAB3477V-100UG
Alexa Fluor 488	FAB3477G-100UG
Alexa Fluor 532	FAB3477X
Alexa Fluor 594	FAB3477T-100UG
Alexa Fluor 647	FAB3477R-100UG
Alexa Fluor 700	FAB3477N-100UG
Alexa Fluor 750	FAB3477S-100UG
Alexa Fluor Plus 405	FAB3477AFP405-100UG
Alexa Fluor Plus 488	FAB3477AFP488-100UG
Alexa Fluor Plus 555	FAB3477AFP555-100UG
Alexa Fluor Plus 594	FAB3477AFP594-100UG
Alexa Fluor Plus 647	FAB3477AFP647-100UG
Alexa Fluor Plus 680	FAB3477AFP680-100UG
Allophycocyanin	FAB3477A-100
Allophycocyanin	FAB3477A-025
Allophycocyanin/Cy7	FAB3477APCCY7
Biotin	FAB3477B
CoraFluor 1	FAB3477CL1
DyLight 350	FAB3477D
DyLight 405	FAB3477E
DyLight 488	FAB3477K
DyLight 550	FAB3477L
DyLight 594	FAB3477M
DyLight 650	FAB3477W
DyLight 680	FAB3477Y
DyLight 755	FAB3477Z
HRP	FAB3477H
Janelia Fluor 525	FAB3477JF525
Janelia Fluor 549	FAB3477I
Janelia Fluor 646	FAB3477J
Janelia Fluor 669	FAB3477JF669
PE/Atto594	FAB3477PEATT594
PE/Cy5.5	FAB3477PECY55
PE/Cy7	FAB3477PECY7
PerCP	FAB3477C
mFluor Violet 450 SE	FAB3477MFV450
mFluor Violet 500 SE	FAB3477MFV500
mFluor Violet 610 SE	FAB3477MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB3477A-025
Carrier Free	FAB3477A-100
Carrier Free	MAB3477-SP
Carrier Free	FAB3477V-100UG
Carrier Free	FAB3477U-100UG
Carrier Free	FAB3477T-100UG
Carrier Free	FAB3477S-100UG
Carrier Free	FAB3477R-100UG
Carrier Free	FAB3477N-100UG
Carrier Free	FAB3477G-100UG

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NBP2-67324

CCR7 Antibody (SR36-04)

(2 Publications)

100 ul

NBP2-67324

Immunohistochemistry-Paraffin: CCR7 Antibody (SR36-04) [NBP2-67324] - Analysis of paraffin-embedded human tonsil tissue using anti-CCR7 antibody. Counter stained with hematoxylin.

<b>Biological Strategies Validation. </b>CCR7-Antibody-SR36-04-Western-Blot-NBP2-67324-img0008.jpg

Immunocytochemistry/Immunofluorescence: CCR7 Antibody (SR36-04) [NBP2-67324] - Staining CCR7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: CCR7 Antibody (SR36-04) [NBP2-67324] - Staining CCR7 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CCR7 Antibody (SR36-04) [NBP2-67324] - Analysis of paraffin-embedded human spleen tissue using anti-CCR7 antibody. Counter stained with hematoxylin.

<b>Genetic Strategies Validation. </b>CCR7-Antibody-SR36-04-Knockdown-Validated-NBP2-67324-img0007.jpg

Western Blot: CCR7 Antibody (SR36-04) [NBP2-67324] - Curcumae radix extract treatment exhibited anti-metastatic effects on primary tumor of MMTV-PyMT transgenic mouse. (A) Quantitative analysis of real-time PCR was done using genes associated with breast cancer metastasis. The expression levels of Ccr7, c-Fos, Mmp9, Ccl21, & Ccl19 were determined by qRT-PCR. The Hprt gene was used as input control, respectively, * p < 0.05 & ** p < 0.005 vs. control. (B) Western blot of proteins extracted from tumor tissues in the PyMT transgenic model against Ccr7 & Gapdh. Histogram data was presented as mean ± SEM. * p < 0.05 vs. control. (C) Western blot of proteins extracted from tumor tissues against Phosphorylated Akt, Phosphorylated Ampk, Her2, ERalpha, Gapdh & beta -actin. (D) Quantitative analysis of real time PCR was done by using genes associated with apoptosis. The expression levels of Jun, Akt, & Tnf were determined by qRT-PCR. Data are representative of three independent experiments & are expressed as the mean ± SD. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30781353), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NLS848

CCR7 Antibody - BSA Free

0.05 ml

NLS848

Immunohistochemistry-Paraffin: CCR7 Antibody [NLS848] - Analysis of anti-CCR7 antibody with human tonsil at dilution: 14 ug/ml

Immunohistochemistry-Paraffin: CCR7 Antibody [NLS848] - Analysis of anti-CCR7 antibody with human spleen at dilution 14 ug/ml.

Reactivity:

Hu, Bt, Bv, Eq, Pm, Pm, Mu, Rt, Ca, Ha, Rb

Species Glossary

Reactivity: Hu, Bt, Bv, Eq, Pm, Pm, Mu, Rt, Ca, Ha, Rb

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-31089

CCR7 Antibody - BSA Free

(3 Publications)

0.05 mg

NBP2-31089

Immunohistochemistry-Paraffin: CCR7 Antibody [NBP2-31089] - Human lymph node tissue after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: CCR7 Antibody [NBP2-31089] - Lymph node, eosinophilic granuloma Langerhans histiocytes.

Reactivity:

Hu, Bv, Pm

Species Glossary

Reactivity: Hu, Bv, Pm

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-71209

Alt Catalog# IMG-71209

Conjugate:

Unconjugated

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NLS847

CCR7 Antibody - BSA Free

0.05 ml

NLS847

Immunohistochemistry-Paraffin: CCR7 Antibody [NLS847] - Analysis of anti-CCR7 antibody with human bone, eosinophilic granuloma.

Immunohistochemistry-Paraffin: CCR7 Antibody [NLS847] - Anti-CCR7 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: CCR7 Antibody [NLS847] - Hodgkin's disease Reed Sternberg cells

Immunohistochemistry-Paraffin: CCR7 Antibody [NLS847] - Brain Hypothalamus Neuron

Immunohistochemistry-Paraffin: CCR7 Antibody [NLS847] - Human lymph node. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Reactivity:

Hu, Bv, Pm, Pm

Species Glossary

Reactivity: Hu, Bv, Pm, Pm

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-71210

Alt Catalog# IMG-71210

Conjugate:

Unconjugated

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NBP2-41251

CCR7 Antibody - BSA Free

(1 Publication)

0.1 mg

NBP2-41251

Immunohistochemistry: CCR7 Antibody - BSA Free [NBP2-41251] - Immunohistochemistry of CCR7 in human spleen tissue with CCR7 antibody at 5 ug/mL.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-41251AF350
Alexa Fluor 405	NBP2-41251AF405
Alexa Fluor 488	NBP2-41251AF488
Alexa Fluor 532	NBP2-41251AF532
Alexa Fluor 594	NBP2-41251AF594
Alexa Fluor 647	NBP2-41251AF647
Alexa Fluor 700	NBP2-41251AF700
Alexa Fluor 750	NBP2-41251AF750
Alexa Fluor Plus 405	NBP2-41251AFP405
Alexa Fluor Plus 488	NBP2-41251AFP488
Alexa Fluor Plus 555	NBP2-41251AFP555
Alexa Fluor Plus 594	NBP2-41251AFP594
Alexa Fluor Plus 647	NBP2-41251AFP647
Alexa Fluor Plus 680	NBP2-41251AFP680
Allophycocyanin	NBP2-41251APC
Biotin	NBP2-41251B
CoraFluor 1	NBP2-41251CL1
DyLight 350	NBP2-41251UV
DyLight 405	NBP2-41251V
DyLight 488	NBP2-41251G
DyLight 550	NBP2-41251R
DyLight 594	NBP2-41251DL594
DyLight 650	NBP2-41251C
DyLight 680	NBP2-41251FR
DyLight 755	NBP2-41251IR
FITC	NBP2-41251F
HRP	NBP2-41251H
Janelia Fluor 525	NBP2-41251JF525
Janelia Fluor 549	NBP2-41251JF549
Janelia Fluor 646	NBP2-41251JF646
Janelia Fluor 669	NBP2-41251JF669
PE	NBP2-41251PE
PerCP	NBP2-41251PCP
mFluor Violet 450 SE	NBP2-41251MFV450
mFluor Violet 500 SE	NBP2-41251MFV500
mFluor Violet 610 SE	NBP2-41251MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-41251-0.025mg

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NBP2-61680

CCR7 Antibody (4B7B8) - BSA Free

0.1 ml

NBP2-61680

Western Blot: CCR7 Antibody (4B7B8) [NBP2-61680] - Analysis using CD197 mAb against human CD197 (AA: extra mix) recombinant protein. (Expected MW is 37.5 kDa)

Western Blot: CCR7 Antibody (4B7B8) [NBP2-61680] - Analysis using CD197 mouse mAb against C6 (1) cell lysate.

Flow Cytometry: CCR7 Antibody (4B7B8) [NBP2-61680] - Analysis of HL-60 cells using CCR7 mouse mAb (green) and negative control (red).

ELISA: CCR7 Antibody (4B7B8) [NBP2-61680] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-61680-0.025ml

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NBP1-43332

CCR7 Antibody (3D12) - BSA Free

0.1 mg

NBP1-43332

Flow Cytometry: CCR7 Antibody (3D12) [NBP1-43332] - Analysis using the Biotin conjugate of NBP1-43332. Staining of normal human peripheral blood cells with Anti-Human CD45RA FITC and 1.0 micrograms of Rat IgG2a k Isotype Control Biotin (left) or 1.0 micrograms of Anti-Human CD197 (CCR7) Biotin (right) followed by Streptavidin PE. Cells in the lymphocyte gate were used for analysis.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-43332AF350
Alexa Fluor 405	NBP1-43332AF405
Alexa Fluor 488	NBP1-43332AF488
Alexa Fluor 532	NBP1-43332AF532
Alexa Fluor 594	NBP1-43332AF594
Alexa Fluor 647	NBP1-43332AF647
Alexa Fluor 700	NBP1-43332AF700
Alexa Fluor 750	NBP1-43332AF750
Alexa Fluor Plus 488	NBP1-43332AFP488
Alexa Fluor Plus 555	NBP1-43332AFP555
Alexa Fluor Plus 594	NBP1-43332AFP594
Alexa Fluor Plus 647	NBP1-43332AFP647
Alexa Fluor Plus 680	NBP1-43332AFP680
Allophycocyanin	NBP1-43011-100tests
Allophycocyanin	NBP1-43332APC
Allophycocyanin/Cy7	NBP1-43332APCCY7
Biotin	NBP1-43332B
CoraFluor 1	NBP1-43332CL1
DyLight 350	NBP1-43332UV
DyLight 405	NBP1-43332V
DyLight 488	NBP1-43332G
DyLight 550	NBP1-43332R
DyLight 594	NBP1-43332DL594
DyLight 650	NBP1-43332C
DyLight 680	NBP1-43332FR
DyLight 755	NBP1-43332IR
FITC	NBP1-43332F
HRP	NBP1-43332H
Janelia Fluor 525	NBP1-43332JF525
Janelia Fluor 549	NBP1-43332JF549
Janelia Fluor 646	NBP1-43332JF646
Janelia Fluor 669	NBP1-43332JF669
PE	NBP1-43332PE
PE/Atto594	NBP1-43332PEATT594
PE/Cy5.5	NBP1-43332PECY55
PE/Cy7	NBP1-43332PECY7
PerCP	NBP1-43332PCP
mFluor Violet 450 SE	NBP1-43332MFV450
mFluor Violet 500 SE	NBP1-43332MFV500
mFluor Violet 610 SE	NBP1-43332MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-43332-25ug

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NBP3-12012

CCR7 Antibody (4B12) - Chimeric - Azide and BSA Free

0.2 mg

NBP3-12012

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-12012AF350
Alexa Fluor 405	NBP3-12012AF405
Alexa Fluor 488	NBP3-12012AF488
Alexa Fluor 532	NBP3-12012AF532
Alexa Fluor 594	NBP3-12012AF594
Alexa Fluor 647	NBP3-12012AF647
Alexa Fluor 700	NBP3-12012AF700
Alexa Fluor 750	NBP3-12012AF750
Alexa Fluor Plus 405	NBP3-12012AFP405
Alexa Fluor Plus 488	NBP3-12012AFP488
Alexa Fluor Plus 555	NBP3-12012AFP555
Alexa Fluor Plus 594	NBP3-12012AFP594
Alexa Fluor Plus 647	NBP3-12012AFP647
Alexa Fluor Plus 680	NBP3-12012AFP680
Allophycocyanin	NBP3-12012APC
Biotin	NBP3-12012B
CoraFluor 1	NBP3-12012CL1
DyLight 350	NBP3-12012UV
DyLight 405	NBP3-12012V
DyLight 488	NBP3-12012G
DyLight 550	NBP3-12012R
DyLight 594	NBP3-12012DL594
DyLight 650	NBP3-12012C
DyLight 680	NBP3-12012FR
DyLight 755	NBP3-12012IR
FITC	NBP3-12012F
HRP	NBP3-12012H
Janelia Fluor 525	NBP3-12012JF525
Janelia Fluor 549	NBP3-12012JF549
Janelia Fluor 646	NBP3-12012JF646
Janelia Fluor 669	NBP3-12012JF669
PE	NBP3-12012PE
PerCP	NBP3-12012PCP
mFluor Violet 450 SE	NBP3-12012MFV450
mFluor Violet 500 SE	NBP3-12012MFV500
mFluor Violet 610 SE	NBP3-12012MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-12012-0.025mg

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NBP2-57375

CCR7 Antibody - BSA Free

100 ul

NBP2-57375

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-57375-25ul

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

CCR7 Products By Gene Name

CCR7 Antibody (150503) [Unconjugated]

CCR7 Antibody

CCR7 Antibody (4B12) [Unconjugated]

CCR7 Antibody (SR36-04)

CCR7 Antibody - BSA Free

CCR7 Antibody - BSA Free

CCR7 Antibody - BSA Free

CCR7 Antibody - BSA Free

CCR7 Antibody (4B7B8) - BSA Free

CCR7 Antibody (3D12) - BSA Free

CCR7 Antibody (4B12) - Chimeric - Azide and BSA Free

CCR7 Antibody - BSA Free