CCR4 Antibody (HL2481) - Azide and BSA Free

Western Blot: CCR4 Antibody (HL2481) - Azide and BSA Free [NBP3-25402] - Non-transfected (-) and transfected (+) bolied and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: CCR4 Antibody (HL2481) - Azide and BSA Free [NBP3-25402] - CCR4 antibody [HL2481] detects CCR4 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded rat thymus gland. CCR4 stained by CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: CCR4 Antibody (HL2481) - Azide and BSA Free [NBP3-25402] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: CCR4 Antibody (HL2481) - Azide and BSA Free [NBP3-25402] - Non-transfected (-) and transfected (+) boiled and unboiled SW480 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: CCR4 Antibody (HL2481) - Azide and BSA Free [NBP3-25402] - Unboiled SW480 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. (WCE: whole cell extract; ME: membrane extract)

Immunohistochemistry-Paraffin: CCR4 Antibody (HL2481) - Azide and BSA Free [NBP3-25402] - CCR4 antibody [HL2481] detects CCR4 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse thymus gland. CCR4 stained by CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ICC/IF: CCR4 Antibody (HL2481) -SW480 cells were fixed in ice-cold MeOH for 5 min.Green: CCR4 stained by CCR4 antibody [HL2481] diluted at 1:200.Blue: Fluoroshield with DAPI.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: HL2481
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CCR4 Antibody (HL2481) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit CCR4 Antibody (HL2481) - Azide and BSA Free (NBP3-25402) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide of human CCR4.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CCR4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Assay dependent
  • Immunohistochemistry Assay dependent
  • Immunohistochemistry-Paraffin Assay dependent
  • Western Blot 1:500-1:3000
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for CCR4 Antibody (HL2481) - Azide and BSA Free

• C-C chemokine receptor type 4
• C-C CKR-4
• CC-CKR-4HGCN:14099
• CCR4
• CCR-4
• CD194 antigen
• CD194
• chemokine (C-C motif) receptor 4
• chemokine (C-C) receptor 4
• ChemR13
• CKR4MGC88293
• CMKBR4k5-5
• K5-5

Background

Chemokine receptors are G protein-coupled receptors (GPCRs) that play a key role in inflammation and are characterized by similar sequences with a 4 cysteine motif. The C-C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation. Receptors for the C-C chemokine family include CCR-1, CCR-2A, CCR-3, CCR-4, CCR-5 and the Duffy blood group antigen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCR4