![Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CNOT8 antibody [HL2586] (NBP3-48703) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48703_rabbit-cnot8-mab-hl2586-western-blot-1411202518335912.jpg "Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CNOT8 antibody [HL2586] (NBP3-48703) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, ZeSpecies Glossary |
| Applications | WB, ICC/IF |
| Clone | HL2586 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Novus Biologicals Rabbit CNOT8 Antibody (HL2586) (NBP3-48703) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant fragment of human CNOT8. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | CNOT8 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
![Simple Western CD117/c-kit Antibody [Unconjugated]](https://images.novusbio.com/images2/CD117_AF1356_Simple_Western_17480.jpg)
![Western Blot CD117/c-kit Antibody [Unconjugated]](https://images.novusbio.com/images2/CD117_AF1356_Western_Blot_18637.jpg)
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Secondary Antibodies |
Isotype Controls |
Research Areas for CNOT8 Antibody (NBP3-48703)Find related products by research area.
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| Gene Symbol | CNOT8 |
![CNOT8 antibody [HL2586] detects CNOT8 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CNOT8 stained by CNOT8 antibody [HL2586] (NBP3-48703) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-48703_rabbit-cnot8-mab-hl2586-immunocytochemistry-immunofluorescence-1411202518435613.jpg "CNOT8 antibody [HL2586] detects CNOT8 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CNOT8 stained by CNOT8 antibody [HL2586] (NBP3-48703) diluted at 1:500.Blue: Fluoroshield with DAPI .")
![Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CNOT8 antibody [HL2586] (NBP3-48703) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48703_rabbit-cnot8-mab-hl2586-western-blot-1411202518404313.jpg "Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CNOT8 antibody [HL2586] (NBP3-48703) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CNOT8 antibody [HL2586] (NBP3-48703) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48703_rabbit-cnot8-mab-hl2586-western-blot-1411202518384116.jpg "Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CNOT8 antibody [HL2586] (NBP3-48703) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 12% SDS-PAGE, and the membrane was blotted with CNOT8 antibody [HL2586] (NBP3-48703) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48703_rabbit-cnot8-mab-hl2586-western-blot-1411202518443419.jpg "Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 12% SDS-PAGE, and the membrane was blotted with CNOT8 antibody [HL2586] (NBP3-48703) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Whole Japanese medaka extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CNOT8 antibody [HL2586] (NBP3-48703) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48703_rabbit-cnot8-mab-hl2586-western-blot-141120251832013.jpg "Whole Japanese medaka extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CNOT8 antibody [HL2586] (NBP3-48703) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")