CCR6 Antibody (53103)

CCR6 was detected in immersion fixed paraffin-embedded sections of human spleen using 25 µg/mL Human CCR6 Monoclonal Antibody (Catalog # MAB195) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: Flow, IHC, CyTOF-ready
• Clone: 53103
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CCR6 Antibody (53103) Summary

• Additional Information: Clone 53103 was used by HLDA to establish CD designation
• Immunogen: NS0 mouse myeloma cell line transfected with human CCR6
  Met1-Met374
  Accession # P51684
• Specificity: Detects human CCR6. Stains human CCR6 transfectants but not the parental cell line in flow cytometry. Does not cross-react with transfectants expressing human CCR1, CCR2, CCR3, CCR4, CCR5, CCR7, CXCR1, CXCR2, CXCR3, CXCR4, or STRL33.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCR6
• Purity: Protein A or G purified from ascites
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from ascites
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR6 Antibody (53103)

• BN-1
• CC-CKR-6
• CCR6
• CCR-6
• CD196 antigen
• CD196
• chemokine (C-C motif) receptor 6
• CKRL3
• CKR-L3
• CKR-L3G-protein coupled receptor 29
• CKRL3LARC receptor
• CMKBR6
• CMKBR6STRL22
• DRY6
• GPR29
• GPR29C-C CKR-6
• GPRCY4
• GPR-CY4
• GPRCY4DRY-6
• lymphocyte, 22
• STRL22

Background

CCR6 is a seven transmembrane-spanning G protein-coupled receptor that binds the chemokine MIP-3 alpha and mediates the chemoattraction of B and T lymphocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR6 Antibody (MAB195)(33)

Publications using MAB195

• A Woestemeie, P Scognamigl, Y Zhao, J Wagner, F Muscate, C Casar, F Siracusa, F Cortesi, T Agalioti, S Müller, A Sagebiel, L Konczalla, R Wahib, KF Karstens, AD Giannou, A Duprée, S Wolter, MN Wong, AK Mühlig, AA Bielecka, V Bansal, T Zhang, O Mann, VG Puelles, TB Huber, AW Lohse, JR Izbicki, NW Palm, S Bonn, S Huber, N Gagliani Multicytokine-producing CD4+ T cells characterize the livers of patients with NASH JCI Insight, 2023-01-10;8(1):. 2023-01-10 [PMID: 36625344] (IHC, Human)
• T Balzano, P Leone, G Ivaylova, MC Castro, L Reyes, C Ramón, M Malaguarne, M Llansola, V Felipo Rifaximin Prevents T-Lymphocytes and Macrophages Infiltration in Cerebellum and Restores Motor Incoordination in Rats with Mild Liver Damage Biomedicines, 2021-08-12;9(8):. 2021-08-12 [PMID: 34440206] (IHC, Rat)
• F Facciotti, P Larghi, R Bosotti, C Vasco, N Gagliani, C Cordiglier, S Mazzara, V Ranzani, E Rottoli, S Curti, A Penatti, B Karnani, Y Kobayashi, M Crosti, M Bombaci, JP van Hambur, G Rossetti, R Gualtierot, M Gerosa, S Gatti, S Torretta, L Pignataro, SW Tas, S Abrignani, M Pagani, F Grassi, PL Meroni, RA Flavell, J Geginat Evidence for a pathogenic role of extrafollicular, IL-10-producing CCR6+B helper T cells in systemic lupus erythematosus Proc. Natl. Acad. Sci. U.S.A., 2020-03-17;117(13):7305-7316. 2020-03-17 [PMID: 32184325] (IHC, Human)
• T Balzano, J Forteza, P Molina, J Giner, A Monzó, J Sancho-Jim, A Urios, C Montoliu, V Felipo The Cerebellum of Patients with Steatohepatitis Shows Lymphocyte Infiltration, Microglial Activation and Loss of Purkinje and Granular Neurons Sci Rep, 2018-02-14;8(1):3004. 2018-02-14 [PMID: 29445232] (IHC-P, Human)
• XP Zhang, ZJ Hu, AH Meng, GC Duan, QT Zhao, J Yang Role of CCL20/CCR6 and the ERK signaling pathway in lung adenocarcinoma Oncol Lett, 2017-10-23;14(6):8183-8189. 2017-10-23 [PMID: 29250193] (IHC-P, Human)
• B Soto, T Gallastegi, C Rodríguez, J Martínez-G, JR Escudero, L Vila, M Camacho Circulating CCL20 as a New Biomarker of Abdominal Aortic Aneurysm Sci Rep, 2017-12-11;7(1):17331. 2017-12-11 [PMID: 29229985] (IHC-P, Human)
• R Diao, T Wang, KL Fok, X Li, Y Ruan, MK Yu, Y Cheng, Y Chen, H Chen, L Mou, X Cai, Y Wang, Z Cai, X Zeng, HC Chan CCR6 is required for ligand-induced CatSper activation in human sperm Oncotarget, 2017-09-05;8(53):91445-91458. 2017-09-05 [PMID: 29207656] (ICC, Human)
• J Dunham, N van Driel, BJ Eggen, C Paul, BA 't Hart, JD Laman, YS Kap Analysis of the cross-talk of Epstein-Barr virus-infected B cells with T cells in the marmoset Clin Transl Immunology, 2017-02-10;6(2):e127. 2017-02-10 [PMID: 28243437] (Flow Cytometry, Primate)
• W Szczeklik, B Jakie?a, K Wawrzycka-, M Sanak, M Hubalewska, A Padjas, M Surmiak, K Szczeklik, J Sznajd, J Musia? Skewing towards Treg and Th2 responses is a characteristic feature of sustained remission in ANCA-positive granulomatosis with polyangiitis Eur. J. Immunol, 2017-02-27;0(0):. 2017-02-27 [PMID: 28155222] (Flow Cytometry, Human)
• Valentina Cecchinato Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization J. Immunol, 2016-11-28;0(0):. 2016-11-28 [PMID: 27895171] (IHC-P, Primate - Macaca mulatta (Rhesus Macaque))
• CCR6 expression in colon cancer is associated with advanced disease and supports epithelial-to-mesenchymal transition Br J Cancer, 2016-05-05;0(0):. 2016-05-05 [PMID: 27149649] (Bioassay, Flow Cytometry, IHC-P, Human)
• Schanz A, Red-Horse K, Hess A, Baston-Bust D, Heiss C, Krussel J Oxygen regulates human cytotrophoblast migration by controlling chemokine and receptor expression. Placenta, 2014-09-26;35(12):1089-94. 2014-09-26 [PMID: 25293376] (Western Blot, Human)
• Liu , Jinlin, Ke , Fang, Xu , Zhenyao, Liu , Zhaoyuan, Zhang , Lingyun, Yan , Sha, Wang , Zhe, Wang , Hong, Wang , Honglin CCR6 is a prognostic marker for overall survival in patients with colorectal cancer, and its overexpression enhances metastasis in vivo. PLoS ONE, 2014-06-30;9(6):e101137. 2014-06-30 [PMID: 24979261] (IHC, Mouse)
• Nandi , Bisweswa, Pai , Christin, Huang , Qin, Prabhala , Rao H, Munshi , Nikhil C, Gold , Jason S CCR6, the sole receptor for the chemokine CCL20, promotes spontaneous intestinal tumorigenesis. PLoS ONE, 2014-05-27;9(5):e97566. 2014-05-27 [PMID: 24866282] (IHC-P, Mouse)
• Malard F, Bossard C, Brissot E, Chevallier P, Guillaume T, Delaunay J, Mosnier J, Moreau P, Gregoire M, Gaugler B, Mohty M Increased Th17/Treg ratio in chronic liver GVHD. Bone Marrow Transplant, 2014-01-13;49(4):539-44. 2014-01-13 [PMID: 24419519] (IHC-P, Human)
• Malard F, Bossard C, Brissot E, Chevallier P, Guillaume T, Delaunay J, Mosnier J, Moreau P, Gregoire M, Gaugler B, Mohty M Increased plasmacytoid dendritic cells and RORgammat-expressing immune effectors in cutaneous acute graft-versus-host disease. J Leukoc Biol, 2013-08-29;94(6):1337-43. 2013-08-29 [PMID: 23990625] (IHC-P, Human)
• Mazerolles F, Picard C, Kracker S, Fischer A, Durandy A Blood CD4+CD45RO+CXCR5+ T cells are decreased but partially functional in signal transducer and activator of transcription 3 deficiency. J Allergy Clin Immunol, 2013-02-10;131(4):1146-56, 1156. 2013-02-10 [PMID: 23403044] (Flow Cytometry, Human)
• Miyagaki T, Sugaya M, Suga H, Kamata M, Ohmatsu H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S IL-22, but not IL-17, dominant environment in cutaneous T-cell lymphoma. Clin. Cancer Res., 2011-11-02;17(24):7529-38. 2011-11-02 [PMID: 22048239] (IHC-P, Human)
• Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA Identification of human NK17/NK1 cells. PLoS ONE, 2011-10-21;6(10):e26780. 2011-10-21 [PMID: 22039549] (Flow Cytometry, Human)
• Kim S, Lewis C, Nadel JA CCL20/CCR6 feedback exaggerates epidermal growth factor receptor-dependent MUC5AC mucin production in human airway epithelial (NCI-H292) cells. J. Immunol., 2011-02-07;186(6):3392-400. 2011-02-07 [PMID: 21300824] (Western Blot, Human)
• Charles J, Di Domizio J, Salameire D, Bendriss-Vermare N, Aspord C, Muhammad R, Lefebvre C, Plumas J, Leccia MT, Chaperot L Characterization of circulating dendritic cells in melanoma: role of CCR6 in plasmacytoid dendritic cell recruitment to the tumor. J. Invest. Dermatol., 2010-03-11;130(6):1646-56. 2010-03-11 [PMID: 20220766] (IHC-P, Human)
• Franciszkiewicz K, Le Floc'h A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadiere C, Mami-Chouaib F Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention. Cancer Res., 2009-07-28;69(15):6249-55. 2009-07-28 [PMID: 19638592] (Flow Cytometry, Human)
• Albanesi C, Scarponi C, Pallotta S, Daniele R, Bosisio D, Madonna S, Fortugno P, Gonzalvo-Feo S, Franssen JD, Parmentier M, De Pita O, Girolomoni G, Sozzani S Chemerin expression marks early psoriatic skin lesions and correlates with plasmacytoid dendritic cell recruitment. J. Exp. Med., 2008-12-29;206(1):249-58. 2008-12-29 [PMID: 19114666] (Blocking, Human)
• Kang SG, Lim HW, Andrisani OM, Broxmeyer HE, Kim CH Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells. J. Immunol., 2007-09-15;179(6):3724-33. 2007-09-15 [PMID: 17785809] (Flow Cytometry, Human)
• Bergeron A, El-Hage F, Kambouchner M, Lecossier D, Tazi A Characterisation of dendritic cell subsets in lung cancer micro-environments. Eur. Respir. J., 2006-08-09;28(6):1170-7. 2006-08-09 [PMID: 16899480] (IHC, Human)
• Marchal-Somme J, Uzunhan Y, Marchand-Adam S, Valeyre D, Soumelis V, Crestani B, Soler P Cutting edge: nonproliferating mature immune cells form a novel type of organized lymphoid structure in idiopathic pulmonary fibrosis. J. Immunol., 2006-05-15;176(10):5735-9. 2006-05-15 [PMID: 16670278] (IHC-Fr, Human)
• Lim HW, Hillsamer P, Kim CH Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses. J. Clin. Invest., 2004-12-01;114(11):1640-9. 2004-12-01 [PMID: 15578096] (ICC, Human)
• Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands. J. Immunol., 2004-12-01;173(11):6592-602. 2004-12-01 [PMID: 15557149] (Flow Cytometry, Human)
• Page G, Miossec P Paired synovium and lymph nodes from rheumatoid arthritis patients differ in dendritic cell and chemokine expression. J. Pathol., 2004-09-01;204(1):28-38. 2004-09-01 [PMID: 15307135] (IHC-P, Human)
• Page G, Sattler A, Kersten S, Thiel A, Radbruch A, Miossec P Plasma cell-like morphology of Th1-cytokine-producing cells associated with the loss of CD3 expression. Am. J. Pathol., 2004-02-01;164(2):409-17. 2004-02-01 [PMID: 14742247] (ICC, IHC-P, Human)
• Annels NE, Willemze AJ, van der Velden VH, Faaij CM, van Wering E, Sie-Go DM, Egeler RM, van Tol MJ, Révész T Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL. Blood, 2003-12-04;103(7):2806-8. 2003-12-04 [PMID: 14656893] (Flow Cytometry, Human)
• Maki W, Morales RE, Carroll VA, Telford WG, Knibbs RN, Stoolman LM, Hwang ST CCR6 colocalizes with CD18 and enhances adhesion to activated endothelial cells in CCR6-transduced Jurkat T cells. J. Immunol., 2002-09-01;169(5):2346-53. 2002-09-01 [PMID: 12193700] (ICC, Human)
• Campbell J, Qin S, Unutmaz D, Soler D, Murphy K, Hodge M, Wu L, Butcher E Unique subpopulations of CD56+ NK and NK-T peripheral blood lymphocytes identified by chemokine receptor expression repertoire. J Immunol, 2001-06-01;166(11):6477-82. 2001-06-01 [PMID: 11359797] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CCR6
• Uniprot:
  • P51684()