Human CCL22/MDC Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human CCL22/MDC Quantikine ELISA Kit Summary

• Background: The Quantikine Human MDC Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human MDC in cell culture supernates, serum, or plasma. It contains E. coli-expressed recombinant human MDC and has been shown to accurately quantitate the recombinant factor. Results obtained using naturally expressed human MDC showed linear curves that were parallel to the standard curves obta...ined using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally expressed human MDC.
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• Specificity: Natural and recombinant human MDC
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CCL22

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CCL22/MDC Quantikine ELISA Kit

• A-152E5.1
• ABCD-1
• CC chemokine STCP-1
• C-C motif chemokine 22
• CCL22
• chemokine (C-C motif) ligand 22
• DC/B-CK
• Macrophage-derived chemokine
• MDC
• MDCStimulated T-cell chemotactic protein 1
• MGC34554
• SCYA22MDC(1-69)
• small inducible cytokine A22
• small inducible cytokine subfamily A (Cys-Cys), member 22
• Small-inducible cytokine A22
• STCP-1
• stimulated T cell chemotactic protein 1

Background

Macrophage-derived chemokine (MDC), also named stimulated T cell chemotactic protein (STCP-1) and ABCD-1, and now designated as CCL22, is a CC chemokine initially isolated from clones of monocyte-derived macrophages. At the amino acid sequence level, MDC shows less than 35% identity to other CC chemokine family members. Human MDC is expressed in dendritic cells, macrophages and activated monocytes. In addition, MDC expression is detected in thymus, lymph node and appendix tissues. At the amino acid sequence level, mouse and human MDC share 64% identity and 83% similarity.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for CCL22/MDC (DMD00)(32)

Publications using DMD00

• Chen, J;Zhan, M;Zhao, Y;Xu, H;Feng, F;Bai, Z;Zhang, K;Fu, L;Wang, F;Cheng, Y;Zhang, X;Liu, Y; GM-CSF potentiates macrophages to retain an inflammatory feature from their circulating monocyte precursors in rheumatoid arthritis Journal of translational medicine 2025-08-07 [PMID: 40775724] (Bioassay, Human)
• Song, HK;Park, SH;Kim, HJ;Jang, S;Choo, BK;Kim, HK;Kim, T; Inhibitory effect of Sanguisorba hakusanensis Makino ethanol extract on atopic dermatitis-like responses in NC/Nga mice and human keratinocytes Scientific reports 2023-09-05 [PMID: 37670127] (Human)
• Nelke, C;Schroeter, CB;Stascheit, F;Huntemann, N;Pawlitzki, M;Willison, AG;R�uber, S;Melzer, N;Distler, U;Tenzer, S;St�hler, K;Roos, A;Meisel, A;Meuth, SG;Ruck, T; Eculizumab treatment alters the proteometabolome beyond the inhibiton of complement JCI insight 2023-05-25 [PMID: 37227781] (Human)
• A Jorapur, LA Marshall, S Jacobson, M Xu, S Marubayash, M Zibinsky, DX Hu, O Robles, JJ Jackson, V Baloche, P Busson, D Wustrow, DG Brockstedt, O Talay, PD Kassner, G Cutler EBV+ tumors exploit tumor cell-intrinsic and -extrinsic mechanisms to produce regulatory T cell-recruiting chemokines CCL17 and CCL22 PloS Pathogens, 2022-01-13;18(1):e1010200. 2022-01-13 [PMID: 35025968] (Human)
• OM Ibrahim, PH Basse, W Jiang, K Guru, G Chatta, P Kalinski NF&kapppaB-Activated COX2/PGE2/EP4 Axis Controls the Magnitude and Selectivity of BCG-Induced Inflammation in Human Bladder Cancer Tissues Cancers, 2021-03-16;13(6):. 2021-03-16 [PMID: 33809455] (Human)
• Q Wang, E Schmoeckel, BP Kost, C Kuhn, A Vattai, T Vilsmaier, S Mahner, D Mayr, U Jeschke, HH Heidegger Higher CCL22+ Cell Infiltration is Associated with Poor Prognosis in Cervical Cancer Patients Cancers (Basel), 2019-12-12;11(12):. 2019-12-12 [PMID: 31842422] (Human)
• S Pekkala, A Keskitalo, E Kettunen, S Lensu, N Nykänen, T Kuopio, O Ritvos, J Hentilä, TA Nissinen, JJ Hulmi Blocking Activin Receptor Ligands Is Not Sufficient to Rescue Cancer-Associated Gut Microbiota-A Role for Gut Microbial Flagellin in Colorectal Cancer and Cachexia? Cancers (Basel), 2019-11-15;11(11):. 2019-11-15 [PMID: 31731747] (Human)
• D Bao, J Zhao, X Zhou, Q Yang, Y Chen, J Zhu, P Yuan, J Yang, T Qin, S Wan, J Xing Mitochondrial fission-induced mtDNA stress promotes tumor-associated macrophage infiltration and HCC progression Oncogene, 2019-03-20;0(0):. 2019-03-20 [PMID: 30894684] (Human)
• TD Jung, SI Choi, SH Choi, BY Cho, WS Sim, SJ Lee, SJ Park, DB Kim, YC Kim, JH Lee, OH Lee Changes in the Anti-Allergic Activities of Sesame by Bioconversion Nutrients, 2018-02-14;10(2):. 2018-02-14 [PMID: 29443928] (Human)
• MR Pranzatell, ED Tate, TJ Allison Case-control, exploratory study of cerebrospinal fluid chemokines/cytokines and lymphocyte subsets in childhood Tourette syndrome with positive streptococcal markers Cytokine, 2017-03-10;96(0):49-53. 2017-03-10 [PMID: 28288328] (Human)
• Zhang W, Zheng X, DU L, Sun J, Shen Z, Shi C, Sun S, Zhang Z, Chen X, Qin M, Liu X, Tao J, Jia L, Fan H, Zhou B, Yu Y, Ying H, Hui L, Liu X, Yi X, Liu X, Zhang L, Duan S High salt primes a specific activation state of macrophages, M(Na). Cell Res, 2015-07-24;25(8):893-910. 2015-07-24 [PMID: 26206316] (Human)
• Shi G, Han J, Liu G, Hao Y, Ma Y, Li T, Wu X, Zhang H, Liu Y, Wang B, Kong Y, Zhou J, Zeng H Expansion of activated regulatory T cells by myeloid-specific chemokines via an alternative pathway in CSF of bacterial meningitis patients. Eur J Immunol, 2013-12-02;44(2):420-30. 2013-12-02 [PMID: 24155161] (Human)
• Pranzatelli , Michael, Tate , Elizabet, McGee , Nathan R, Travelstead , Anna L, Colliver , Jerry A, Ness , Jayne M, Ransohoff , Richard BAFF/APRIL system in pediatric OMS: relation to severity, neuroinflammation, and immunotherapy. J Neuroinflammation, 2013-01-16;10(0):10. 2013-01-16 [PMID: 23324534] (Human)
• Rai AK, Thakur CP, Singh A Regulatory T cells suppress T cell activation at the pathologic site of human visceral leishmaniasis. PLoS ONE, 2012-02-08;7(2):e31551. 2012-02-08 [PMID: 22347492] (Human)
• Watanabe T, Asano N, Fichtner-Feigl S, Gorelick PL, Tsuji Y, Matsumoto Y, Chiba T, Fuss IJ, Kitani A, Strober W NOD1 contributes to mouse host defense against Helicobacter pylori via induction of type I IFN and activation of the ISGF3 signaling pathway. J. Clin. Invest., 2010-04-12;120(5):1645-62. 2010-04-12 [PMID: 20389019] (Human)
• Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver. J. Immunol., 2010-02-17;184(6):2886-98. 2010-02-17 [PMID: 20164417] (Human)
• Narbutt J, Lesiak A, Sysa-Jedrzeiowska A, Zakrzewski M, Bogaczewicz J, Stelmach I, Kuna P The imbalance in serum concentration of Th-1- and Th-2-derived chemokines as one of the factors involved in pathogenesis of atopic dermatitis. Mediators Inflamm., 2009-07-22;2009(0):269541. 2009-07-22 [PMID: 19639049] (Human)
• Schuster C, Vaculik C, Fiala C, Meindl S, Brandt O, Imhof M, Stingl G, Eppel W, Elbe-Burger A HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells. J. Exp. Med., 2009-01-12;206(1):169-81. 2009-01-12 [PMID: 19139172] (Human)
• Olsnes AM, Ryningen A, Ersvaer E, Bruserud Ø In vitro induction of a dendritic cell phenotype in primary human acute myelogenous leukemia (AML) blasts alters the chemokine release profile and increases the levels of T cell chemotactic CCL17 and CCL22. J. Interferon Cytokine Res., 2008-05-01;28(5):297-310. 2008-05-01 [PMID: 18547160] (Human)
• Maru SV, Holloway KA, Flynn G, Lancashire CL, Loughlin AJ, Male DK, Romero IA Chemokine production and chemokine receptor expression by human glioma cells: role of CXCL10 in tumour cell proliferation. J. Neuroimmunol., 2008-06-09;199(1):35-45. 2008-06-09 [PMID: 18538864] (Human)
• Haas J, Schopp L, Storch-Hagenlocher B, Fritzsching B, Jacobi C, Milkova L, Fritz B, Schwarz A, Suri-Payer E, Hensel M, Wildemann B Specific recruitment of regulatory T cells into the CSF in lymphomatous and carcinomatous meningitis. Blood, 2007-10-29;111(2):761-6. 2007-10-29 [PMID: 17967942] (Human)
• Martinez de la Torre Y, Buracchi C, Borroni EM, Dupor J, Bonecchi R, Nebuloni M, Pasqualini F, Doni A, Lauri E, Agostinis C, Bulla R, Cook DN, Haribabu B, Meroni P, Rukavina D, Vago L, Tedesco F, Vecchi A, Lira SA, Locati M, Mantovani A Protection against inflammation- and autoantibody-caused fetal loss by the chemokine decoy receptor D6. Proc. Natl. Acad. Sci. U.S.A., 2007-02-05;104(7):2319-24. 2007-02-05 [PMID: 17283337] (Human)
• Borrelli V, di Marzo L, Sapienza P, Colasanti M, Moroni E, Cavallaro A Role of platelet-derived growth factor and transforming growth factor beta1 the in the regulation of metalloproteinase expressions. Surgery, 2006-09-01;140(3):454-63. 2006-09-01 [PMID: 16934609] (Human)
• Ancuta P, Autissier P, Wurcel A, Zaman T, Stone D, Gabuzda D CD16+ monocyte-derived macrophages activate resting T cells for HIV infection by producing CCR3 and CCR4 ligands. J. Immunol., 2006-05-15;176(10):5760-71. 2006-05-15 [PMID: 16670281] (Human)
• Hartl D, Latzin P, Zissel G, Krane M, Krauss-Etschmann S, Griese M Chemokines indicate allergic bronchopulmonary aspergillosis in patients with cystic fibrosis. Am. J. Respir. Crit. Care Med., 2006-03-16;173(12):1370-6. 2006-03-16 [PMID: 16543550] (Human)
• Choi ES, Jakubzick C, Carpenter KJ, Kunkel SL, Evanoff H, Martinez FJ, Flaherty KR, Toews GB, Colby TV, Kazerooni EA, Gross BH, Travis WD, Hogaboam CM Enhanced monocyte chemoattractant protein-3/CC chemokine ligand-7 in usual interstitial pneumonia. Am. J. Respir. Crit. Care Med., 2004-06-10;170(5):508-15. 2004-06-10 [PMID: 15191918] (Human)
• Leung TF, Wong GW, Ko FW, Lam CW, Fok TF Increased macrophage-derived chemokine in exhaled breath condensate and plasma from children with asthma. Clin. Exp. Allergy, 2004-05-01;34(5):786-91. 2004-05-01 [PMID: 15144472] (Human)
• Hon KL, Leung TF, Ma KC, Li AM, Wong Y, Fok TF Serum levels of cutaneous T-cell attracting chemokine (CTACK) as a laboratory marker of the severity of atopic dermatitis in children. Clin. Exp. Dermatol., 2004-05-01;29(3):293-6. 2004-05-01 [PMID: 15115514] (Human)
• Leung TF, Ng PC, Tam WH, Li CY, Wong E, Ma TP, Lam CW, Fok TF Helper T-lymphocyte-related chemokines in healthy newborns. Pediatr. Res., 2003-11-19;55(2):334-8. 2003-11-19 [PMID: 14630994] (Human)
• Albanesi C, Scarponi C, Sebastiani S, Cavani A, Federici M, Sozzani S, Girolomoni G A cytokine-to-chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases. J. Leukoc. Biol., 2001-10-01;70(4):617-23. 2001-10-01 [PMID: 11590199] (Human)
• Thompson SD, Luyrink LK, Graham TB, Tsoras M, Ryan M, Passo MH, Glass DN Chemokine receptor CCR4 on CD4+ T cells in juvenile rheumatoid arthritis synovial fluid defines a subset of cells with increased IL-4:IFN-gamma mRNA ratios. J. Immunol., 2001-06-01;166(11):6899-906. 2001-06-01 [PMID: 11359851] (Human)
• de Lavareille A, Roufosse F, Schandene L, Stordeur P, Cogan E, Goldman M Clonal Th2 cells associated with chronic hypereosinophilia: TARC-induced CCR4 down-regulation in vivo. Eur. J. Immunol., 2001-04-01;31(4):1037-46. 2001-04-01 [PMID: 11298328] (Human)

FAQs for CCL22/MDC (DMD00). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CCL22