FAB3791A) and either (A) Mouse Anti-Human CCR4 Monoclonal Antibody (Catalog # MAB1567) or (B) Mouse IgG2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." alt="Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A) and either (A) Mouse Anti-Human CCR4 Monoclonal Antibody (Catalog # MAB1567) or (B) Mouse IgG2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56336SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86584-25ul | |||
HAF016). A specific band was detected for CCR4 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 8." alt="Western Blot shows lysates of EL‑4 mouse lymphoblast cell line. PVDF membrane was probed with 1 µg/ml of Sheep Anti-Mouse CCR4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5207) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CCR4 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 8." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5207-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81270SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26690-50ul | |||
![Western Blot: CCR4 Antibody [NB100-56336] - Analysis of CCR4 in A) Daudi and B) NIH 3T3 cell lysate using this antibody.](http://images.novusbio.com/fullsize/CCR4-Antibody-Western-Blot-NB100-56336-img0002.jpg "Western Blot: CCR4 Antibody [NB100-56336] - Analysis of CCR4 in A) Daudi and B) NIH 3T3 cell lysate using this antibody.")
![Western Blot: CCR4 Antibody (HL2481) - Azide and BSA Free [NBP3-25402] - Non-transfected (-) and transfected (+) bolied and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25402_rabbit-ccr4-mab-hl2481-azide-and-bsa-free-2120249425444.jpg "Western Blot: CCR4 Antibody (HL2481) - Azide and BSA Free [NBP3-25402] - Non-transfected (-) and transfected (+) bolied and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: CCR4 Antibody (HL2481) - Azide and BSA Free [NBP3-25402] - CCR4 antibody [HL2481] detects CCR4 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded rat thymus gland. CCR4 stained by CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25402_rabbit-ccr4-mab-hl2481-azide-and-bsa-free-2120249351349.jpg)
![Western Blot: CCR4 Antibody (HL2481) - Azide and BSA Free [NBP3-25402] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25402_rabbit-ccr4-mab-hl2481-azide-and-bsa-free-2120249413373.jpg)
![Western Blot: CCR4 Antibody (HL2481) - Azide and BSA Free [NBP3-25402] - Non-transfected (-) and transfected (+) boiled and unboiled SW480 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25402_rabbit-ccr4-mab-hl2481-azide-and-bsa-free-2120249351389.jpg)
![Western Blot: CCR4 Antibody (HL2481) - Azide and BSA Free [NBP3-25402] - Unboiled SW480 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. (WCE: whole cell extract; ME: membrane extract)](http://images.novusbio.com/fullsize/nbp3-25402_rabbit-ccr4-mab-hl2481-azide-and-bsa-free-2120249355539.jpg)
![Immunohistochemistry-Paraffin: CCR4 Antibody (HL2481) - Azide and BSA Free [NBP3-25402] - CCR4 antibody [HL2481] detects CCR4 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse thymus gland. CCR4 stained by CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25402_rabbit-ccr4-mab-hl2481-azide-and-bsa-free-2120249371563.jpg)
![<b>Genetic Strategies Validation. </b>CCR4 antibody [HL2481] detects CCR4 protein by immunofluorescent analysis.</br>Sample: SW480 cells were fixed in ice-cold MeOH for 5 min.</br>Green: CCR4 stained by CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:200.</br>Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25402_rabbit-ccr4-mab-hl2481-azide-and-bsa-free-immunocytochemistry-immunofluorescence-2611202518342622.jpg)
![CCR4 antibody [HL2481] detects CCR4 protein by immunohistochemical analysis.</br>Sample: Paraffin-embedded human tonsil.</br>CCR4 stained by CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:500.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25402_rabbit-ccr4-mab-hl2481-azide-and-bsa-free-immunohistochemistry-paraffin-26112025196213.jpg)
![Unboiled non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25402_rabbit-ccr4-mab-hl2481-azide-and-bsa-free-western-blot-2611202518473213.jpg)
![CCR4 antibody [HL2481] detects CCR4 protein by immunohistochemical analysis.</br>Sample: Paraffin-embedded mouse thymus gland.</br>CCR4 stained by CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:500.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25402_rabbit-ccr4-mab-hl2481-azide-and-bsa-free-immunohistochemistry-paraffin-2611202518331024.jpg)
![Immunohistochemistry-Paraffin: CCR4 Antibody [NBP1-86584] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/CCR4-Antibody-Immunohistochemistry-Paraffin-NBP1-86584-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CCR4 Antibody [NBP1-86584] - Staining of human cerebral cortex shows no positivity in neurons as expected.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CCR4 Antibody [NBP1-86584] - Staining of human rectum shows strong cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CCR4-Antibody-Immunohistochemistry-Paraffin-NBP1-86584-img0011.jpg)
![Immunohistochemistry-Paraffin: CCR4 Antibody [NBP1-86584] - Staining of human skeletal muscle shows no positivity in striated muscle fibers, as expected.](http://images.novusbio.com/fullsize/CCR4-Antibody-Immunohistochemistry-Paraffin-NBP1-86584-img0012.jpg)
![Immunohistochemistry-Paraffin: CCR4 Antibody [NBP1-86584] - Staining of human tonsil shows moderate to strong cytoplasmic positivity in a subset of lymphoid cells.](http://images.novusbio.com/fullsize/CCR4-Antibody-Immunohistochemistry-Paraffin-NBP1-86584-img0013.jpg)
![Immunohistochemistry-Paraffin: CCR4 Antibody [NB100-717] - Section of human spleen.](http://images.novusbio.com/fullsize/CCR4-Antibody-Immunohistochemistry-NB100-717-img0001.jpg "Immunohistochemistry-Paraffin: CCR4 Antibody [NB100-717] - Section of human spleen.")
![ELISA: Human CCR4 ELISA Kit (Colorimetric) [NBP2-75115] - Samples were spiked with high concentrations of Human CCR4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75115_human-ccr4-elisa-kit-colorimetric-1322024164420.jpg "ELISA: Human CCR4 ELISA Kit (Colorimetric) [NBP2-75115] - Samples were spiked with high concentrations of Human CCR4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human CCR4 ELISA Kit (Colorimetric) [NBP2-75115] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75115_human-ccr4-elisa-kit-colorimetric-432024973665.jpg)
![Western Blot: CCR4 Antibody (KW-0761 (Mogamulizumab)) - Chimeric [NBP2-81270] - Western Blot using CCR4 Antibody (KW-0761 (Mogamulizumab)) [NBP2-81270]. Human brain cerebellum (A) and human heart (B) lysates (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of KW-0761 [NBP2-81270] at 2 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for CCR4 is 37.7kDa.](http://images.novusbio.com/fullsize/CCR4-Antibody-KW-0761-Mogamulizumab-Chimeric-Western-Blot-NBP2-81270-img0001.jpg "Western Blot: CCR4 Antibody (KW-0761 (Mogamulizumab)) - Chimeric [NBP2-81270] - Western Blot using CCR4 Antibody (KW-0761 (Mogamulizumab)) [NBP2-81270]. Human brain cerebellum (A) and human heart (B) lysates (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of KW-0761 [NBP2-81270] at 2 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for CCR4 is 37.7kDa.")
![ELISA: CCR4 Antibody (12F5) [NBP3-26690] - The Binding Activity of CCR4 with CCR4 Antibody.<br /> Activity: Measured by its binding ability in a functional ELISA. Immobilized CCR4 at 10 ug/mL can bind CCR4 Antibody, the EC50 is 362.3-630.8 ng/mL.](http://images.novusbio.com/fullsize/nbp3-26690_human-ccr4-mab-12f5-262202412272326.jpg "ELISA: CCR4 Antibody (12F5) [NBP3-26690] - The Binding Activity of CCR4 with CCR4 Antibody.<br /> Activity: Measured by its binding ability in a functional ELISA. Immobilized CCR4 at 10 ug/mL can bind CCR4 Antibody, the EC50 is 362.3-630.8 ng/mL.")
![Flow Cytometry: CCR4 Antibody (12F5) [NBP3-26690] - Untransfected HEK293 cells (green line) and transfected Human CCR4 HEK293 stable cells (red line) were stained with anti-CCR4 antibody(2ul/1*10^6 cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.](http://images.novusbio.com/fullsize/nbp3-26690_human-ccr4-mab-12f5-282202414312746.jpg)
