CCR4 Antibody (205410) [Unconjugated]

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB3791A) and either (A) Mouse Anti-Human CCR4 Monoclonal Antibody (Catalog # MAB1567) or (B) Mouse IgG2B Flow Cytometry Isotype Control (Catalog # MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-reported
• Clone: 205410
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CCR4 Antibody (205410) [Unconjugated] Summary

• Additional Information: Clone 205410 was used by HLDA to establish CD designation
• Immunogen: Human CCR4 transfectants
  Met1-Leu360
  Accession # P51679
• Specificity: Stains human CCR4-transfected cell lines but not the corresponding parent cell lines. Also detects CCR4 on human PBLs and platelets.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCR4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-reported
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR4 Antibody (205410) [Unconjugated]

• C-C chemokine receptor type 4
• C-C CKR-4
• CC-CKR-4HGCN:14099
• CCR4
• CCR-4
• CD194 antigen
• CD194
• chemokine (C-C motif) receptor 4
• chemokine (C-C) receptor 4
• ChemR13
• CKR4MGC88293
• CMKBR4k5-5
• K5-5

Background

CCR4 is a G protein-linked seven transmembrane domain chemokine receptor that binds the chemokines CCL17/TARC and CCL22/MDC. Current evidence suggests that CCR4 expression is associated with Th-2 type T cells and with platelets. CCR4 expression has also been reported in mature dendritic cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR4 Antibody (MAB1567)(19)

Publications using MAB1567

• MH Nelson, HM Knochelman, SR Bailey, LW Huff, JS Bowers, K Majchrzak-, MM Wyatt, MP Rubinstein, S Mehrotra, MI Nishimura, KE Armeson, PG Giresi, MJ Zilliox, HE Broxmeyer, CM Paulos Identification of human CD4+ T cell populations with distinct antitumor activity Science Advances, 2020;6(27):. 2020 [PMID: 32937437] (Flow Cytometry, Human)
• M Martin-Fer, M Bravo Garc, C Gruber, S Murias Loz, MNH Malik, F Alsohime, A Alakeel, R Valdez, S Buta, G Buda, MA Marti, M Larralde, B Boisson, M Feito Rodr, X Qiu, M Chrabieh, M Al Ayed, S Al Muhsen, JV Desai, EMN Ferre, SD Rosenzweig, B Amador-Bor, LY Bravo-Gall, R Olmer, S Merkert, M Bret, AK Sood, A Al-Rabiaah, MH Temsah, R Halwani, M Hernandez, F Pessler, JL Casanova, J Bustamante, MS Lionakis, D Bogunovic Systemic Type I IFN Inflammation in Human ISG15 Deficiency Leads to Necrotizing Skin Lesions Cell Rep, 2020;31(6):107633. 2020 [PMID: 32402279] (CyTof, Human)
• D Michlmayr, EY Kim, AH Rahman, R Raghunatha, S Kim-Schulz, Y Che, S Kalayci, ZH Gümü?, G Kuan, A Balmaseda, A Kasarskis, SM Wolinsky, M Suaréz-Far, E Harris Comprehensive Immunoprofiling of Pediatric Zika Reveals Key Role for Monocytes in the Acute Phase and No Effect of Prior Dengue Virus Infection Cell Rep, 2020;31(4):107569. 2020 [PMID: 32348760] (CyTof, Human)
• NM Bath, X Ding, NA Wilson, BM Verhoven, BA Boldt, A Sukhwal, SR Reese, SE Panzer, A Djamali, RR Redfield Desensitization and treatment with APRIL/BLyS blockade in rodent kidney transplant model PLoS ONE, 2019;14(2):e0211865. 2019 [PMID: 30735519] (Flow Cytometry, Rat)
• Evan W Newell Optimization of mass cytometry sample cryopreservation after staining Cytometry A, 2016;0(0):. 2016 [PMID: 27798817] (CyTof, Human)
• Kap , Yolanda, van Driel , Nikki, Laman , Jon D, Tak , Paul P, 't Hart , Bert A CD20+ B cell depletion alters T cell homing. J Immunol, 2014;192(9):4242-53. 2014 [PMID: 24696233] (Flow Cytometry, Marmoset)
• Aruga A, Takeshita N, Kotera Y, Okuyama R, Matsushita N, Ohta T, Takeda K, Yamamoto M Long-term Vaccination with Multiple Peptides Derived from Cancer-Testis Antigens Can Maintain a Specific T-cell Response and Achieve Disease Stability in Advanced Biliary Tract Cancer. Clin Cancer Res, 2013;19(8):2224-31. 2013 [PMID: 23479678] (Flow Cytometry, Human)
• Duluc , D, Gannevat , J, Anguiano , E, Zurawski , S, Carley , M, Boreham , M, Stecher , J, Dullaers , M, Banchereau , J, Oh , S Functional diversity of human vaginal APC subsets in directing T-cell responses. Mucosal Immunol, 2013;6(3):626-38. 2013 [PMID: 23131784] (Flow Cytometry, Human)
• Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA Identification of human NK17/NK1 cells. PLoS ONE, 2011;6(10):e26780. 2011 [PMID: 22039549] (Flow Cytometry, Human)
• Islam SA, Chang DS, Colvin RA Mouse CCL8, a CCR8 agonist, promotes atopic dermatitis by recruiting IL-5+ T(H)2 cells. Nat. Immunol., 2011;12(2):167-77. 2011 [PMID: 21217759] (Flow Cytometry, Human)
• Fifadara NH, Aye CC, Raghuwanshi SK, Richardson RM, Ono SJ CCR1 expression and signal transduction by murine BMMC results in secretion of TNF-alpha, TGFbeta-1 and IL-6. Int. Immunol., 2009;21(8):991-1001. 2009 [PMID: 19592420] (Flow Cytometry, Mouse)
• Nakamura M, Jo J, Tabata Y, Ishikawa O Controlled delivery of T-box21 small interfering RNA ameliorates autoimmune alopecia (Alopecia Areata) in a C3H/HeJ mouse model. Am. J. Pathol., 2008;172(3):650-8. 2008 [PMID: 18245811] (IHC, Human)
• Kang SG, Lim HW, Andrisani OM, Broxmeyer HE, Kim CH Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells. J. Immunol., 2007;179(6):3724-33. 2007 [PMID: 17785809] (Flow Cytometry, Human)
• Ponte AL, Marais E, Gallay N, Langonne A, Delorme B, Herault O, Charbord P, Domenech J The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities. Stem Cells, 2007;25(7):1737-45. 2007 [PMID: 17395768] (Flow Cytometry, Human)
• Zola H, Swart B, Banham A, Barry S, Beare A, Bensussan A, Boumsell L, D Buckley C, Buhring HJ, Clark G, Engel P, Fox D, Jin BQ, Macardle PJ, Malavasi F, Mason D, Stockinger H, Yang X CD molecules 2006--human cell differentiation molecules. J. Immunol. Methods, 2007;319(1):1-5. 2007 [PMID: 17174972] (Flow Cytometry, Human)
• Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines. Clin. Immunol., 2006;120(2):220-31. 2006 [PMID: 16782408] (Neutralization, Human)
• Marchal-Somme J, Uzunhan Y, Marchand-Adam S, Valeyre D, Soumelis V, Crestani B, Soler P Cutting edge: nonproliferating mature immune cells form a novel type of organized lymphoid structure in idiopathic pulmonary fibrosis. J. Immunol., 2006;176(10):5735-9. 2006 [PMID: 16670278] (IHC-Fr, Human)
• Ohshima K, Karube K, Kawano R, Tsuchiya T, Suefuji H, Yamaguchi T, Suzumiya J, Kikuchii M Classification of distinct subtypes of peripheral T-cell lymphoma unspecified, identified by chemokine and chemokine receptor expression: Analysis of prognosis. Int. J. Oncol., 2004;25(3):605-13. 2004 [PMID: 15289861] (ICC, Human)
• Nakayama EE, Tanaka Y, Nagai Y, Iwamoto A, Shioda T A CCR2-V64I polymorphism affects stability of CCR2A isoform. AIDS, 2004;18(5):729-38. 2004 [PMID: 15075507] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CCR4
• Uniprot:
  • P51679()