CCR4 Antibody (205410) [Fluorescein]

Human peripheral blood lymphocytes were stained with Mouse Anti-Human/Rat CCR4 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1567F) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC0041F). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu, Rt
• Applications: Flow
• Clone: 205410
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Fluorescein

CCR4 Antibody (205410) [Fluorescein] Summary

• Additional Information: Clone 205410 was used by HLDA to establish CD designation
• Immunogen: Human CCR4 transfectants
  Met1-Leu360
  Accession # P51679
• Specificity: Stains human CCR4-transfected cell lines but not the corresponding parent cell lines. Also detects human CCR4 on human PBLs and platelets and rat CCR4 on rat splenocytes.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCR4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR4 Antibody (205410) [Fluorescein]

• C-C chemokine receptor type 4
• C-C CKR-4
• CC-CKR-4HGCN:14099
• CCR4
• CCR-4
• CD194 antigen
• CD194
• chemokine (C-C motif) receptor 4
• chemokine (C-C) receptor 4
• ChemR13
• CKR4MGC88293
• CMKBR4k5-5
• K5-5

Background

CCR4 is a G protein-linked seven transmembrane domain chemokine receptor that binds the chemokines CCL17/TARC and CCL22/MDC. Current evidence suggests that CCR4 expression is associated with Th-2 type T cells and with platelets. CCR4 expression has also been reported in mature dendritic cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR4 Antibody (FAB1567F)(14)

Publications using FAB1567F

• Wang, Z;Ma, J;Zhang, H;Ramakrishna, R;Mintzlaff, D;Mathes, DW;Pomfret, EA;Lucia, MS;Gao, D;Haverkos, BM;Wang, Z; CCR4-IL2 bispecific immunotoxin is more effective than brentuximab for targeted therapy of cutaneous T-cell lymphoma in a mouse CTCL model FEBS open bio 2023-05-08 [PMID: 37157185] (Flow Cytometry, Human)
• L Amniai, C Ple, M Barrier, P de Nadai, P Marquillie, H Vorng, C Chenivesse, A Tsicopoulo, C Duez Natural Killer Cells from Allergic Donors Are Defective in Their Response to CCL18 Chemokine International Journal of Molecular Sciences, 2021-04-09;22(8):. 2021-04-09 [PMID: 33918621] (Flow Cytometry, Human)
• Haoyu Wang, Zhaohui Wang, Huiping Zhang, Zeng Qi, Ariel C. Johnson, David Mathes et al. Bispecific human IL2‐CCR4 immunotoxin targets human cutaneous T‐cell lymphoma Molecular Oncology 5/1/2020 [PMID: 32107846]
• A Kitadate, S Ikeda, F Abe, N Takahashi, N Shimizu, K Matsue, H Tagawa Histone deacetylase inhibitors downregulate CCR4 expression and decrease mogamulizumab efficacy in CCR4-positive mature T-cell lymphomas Haematologica, 2017-10-12;0(0):. 2017-10-12 [PMID: 29025909] (Flow Cytometry, Human)
• Zhirui Wang Porcine Treg depletion with a novel diphtheria toxin-based anti-human CCR4 immunotoxin Vet. Immunol. Immunopathol., 2016-10-29;182(0):150-158. 2016-10-29 [PMID: 27863545] (Flow Cytometry, Porcine)
• Zaidoon Al-Jaderi Glatiramer Acetate, Dimethyl Fumarate, and Monomethyl Fumarate Upregulate the Expression of CCR10 on the Surface of Natural Killer Cells and Enhance Their Chemotaxis and Cytotoxicity Front Immunol, 2016-10-19;7(0):437. 2016-10-19 [PMID: 27807435] (Flow Cytometry, Human)
• James E, Gates T, LaFond R, Yamamoto S, Ni C, Mai D, Gersuk V, O'Brien K, Nguyen Q, Zeitner B, Lanteri M, Norris P, Chaussabel D, Malhotra U, Kwok W Neuroinvasive West Nile Infection Elicits Elevated and Atypically Polarized T Cell Responses That Promote a Pathogenic Outcome. PLoS Pathog, 2016-01-21;12(1):e1005375. 2016-01-21 [PMID: 26795118] (Flow Cytometry, Human)
• Zhaohui Wang, Shannon G. Pratts, Huiping Zhang, Philip J. Spencer, Ruichao Yu, Makoto Tonsho et al. Treg depletion in non-human primates using a novel diphtheria toxin-based anti-human CCR4 immunotoxin Molecular Oncology 4/1/2016 [PMID: 26643572]
• Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA Identification of human NK17/NK1 cells. PLoS ONE, 2011-10-21;6(10):e26780. 2011-10-21 [PMID: 22039549] (Flow Cytometry, Human)
• Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver. J. Immunol., 2010-02-17;184(6):2886-98. 2010-02-17 [PMID: 20164417] (Flow Cytometry, Human)
• Brucklacher-Waldert V, Steinbach K, Lioznov M, Kolster M, Holscher C, Tolosa E Phenotypical characterization of human Th17 cells unambiguously identified by surface IL-17A expression. J. Immunol., 2009-11-01;183(9):5494-501. 2009-11-01 [PMID: 19843935] (Flow Cytometry, Human)
• Finney OC, Nwakanma D, Conway DJ, Walther M, Riley EM Homeostatic regulation of T effector to Treg ratios in an area of seasonal malaria transmission. Eur. J. Immunol., 2009-05-01;39(5):1288-300. 2009-05-01 [PMID: 19338000] (Flow Cytometry, Human)
• Baatar D, Olkhanud P, Newton D, Sumitomo K, Biragyn A CCR4-expressing T cell tumors can be specifically controlled via delivery of toxins to chemokine receptors. J. Immunol., 2007-08-01;179(3):1996-2004. 2007-08-01 [PMID: 17641067] (Flow Cytometry, Human)
• Garcia G, Humbert M, Capel F, Rimaniol AC, Escourrou P, Emilie D, Godot V Chemokine receptor expression on allergen-specific T cells in asthma and allergic bronchopulmonary aspergillosis. Allergy, 2007-02-01;62(2):170-7. 2007-02-01 [PMID: 17298426] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CCR4
• Uniprot:
  • P51679()