CCR4 Antibody (205410) [Phycoerythrin]

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human/Rat CCR4 PE-conjugated Monoclonal Antibody (Catalog # FAB1567P) or (B) Mouse IgG2BPhycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

Rat peripheral blood cells were stained with Mouse Anti-Human/Rat CCR4 PE-conjugated Monoclonal Antibody (Catalog # FAB1567P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu, Rt
• Applications: Flow
• Clone: 205410
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

CCR4 Antibody (205410) [Phycoerythrin] Summary

• Additional Information: Clone 205410 was used by HLDA to establish CD designation
• Immunogen: Human CCR4 transfectants
  Met1-Leu360
  Accession # P51679
• Specificity: Stains human CCR4-transfected cell lines but not the corresponding parent cell lines. Also detects human CCR4 on human PBLs and platelets and rat CCR4 on rat splenocytes.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCR4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR4 Antibody (205410) [Phycoerythrin]

• C-C chemokine receptor type 4
• C-C CKR-4
• CC-CKR-4HGCN:14099
• CCR4
• CCR-4
• CD194 antigen
• CD194
• chemokine (C-C motif) receptor 4
• chemokine (C-C) receptor 4
• ChemR13
• CKR4MGC88293
• CMKBR4k5-5
• K5-5

Background

CCR4 is a G protein-linked seven transmembrane domain chemokine receptor that binds the chemokines CCL17/TARC and CCL22/MDC. Current evidence suggests that CCR4 expression is associated with Th-2 type T cells and with platelets. CCR4 expression has also been reported in mature dendritic cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR4 Antibody (FAB1567P)(21)

Publications using FAB1567P

• JA Villegas, J Van Wassen, J Merrheim, K Matta, S Hamadache, C Flaugère, P Pothin, F Truffault, S Hascoët, N Santelmo, M Alifano, S Berrih-Akn, R le Panse, N Dragin Blocking interleukin-23 ameliorates neuromuscular and thymic defects in myasthenia gravis Journal of Neuroinflammation, 2023-01-13;20(1):9. 2023-01-13 [PMID: 36639663] (Flow Cytometry, Human)
• E Armas-Gonz, MJ Domínguez-, A Díaz-Martí, M Arce-Franc, J Castro-Her, G Danelon, V Hernández-, S Bustabad-R, A Cantabrana, M Uguccioni, F Díaz-Gonzá Role of CXCL13 and CCL20 in the recruitment of B cells to inflammatory foci in chronic arthritis Arthritis Res. Ther., 2018-06-07;20(1):114. 2018-06-07 [PMID: 29880013] (Flow Cytometry, Human)
• Berlato C, Khan MN, Schioppa T et al. A CCR4 antagonist reverses the tumor-promoting microenvironment of renal cancer. J. Clin. Invest. 3/1/2017 [PMID: 28134623]
• Zhirui Wang Porcine Treg depletion with a novel diphtheria toxin-based anti-human CCR4 immunotoxin Vet. Immunol. Immunopathol., 2016-10-29;182(0):150-158. 2016-10-29 [PMID: 27863545] (Flow Cytometry, Porcine)
• Lee Faulkner Detection of Primary T Cell Responses to Drugs and Chemicals in HLA-Typed Volunteers: Implications for the Prediction of Drug Immunogenicity Toxicol. Sci, 2016-09-16;154(2):416-429. 2016-09-16 [PMID: 27637899] (Flow Cytometry, Human)
• Grégory Seumois Transcriptional Profiling of Th2 Cells Identifies Pathogenic Features Associated with Asthma J Immunol, 2016-06-06;0(0):. 2016-06-06 [PMID: 27271570] (Flow Cytometry, Human)
• Zhaohui Wang, Shannon G. Pratts, Huiping Zhang, Philip J. Spencer, Ruichao Yu, Makoto Tonsho et al. Treg depletion in non-human primates using a novel diphtheria toxin-based anti-human CCR4 immunotoxin Molecular Oncology 2016-04-01 [PMID: 26643572]
• Yang , Yongmei, Du , Lutao, Yang , Xiaoyun, Qu , Ailin, Zhang , Xin, Zhou , Chengjun, Wang , Chuanxin Aberrant CCR4 expression is involved in tumor invasion of lymph node-negative human gastric cancer. PLoS ONE, 2015-03-19;10(3):e0120059. 2015-03-19 [PMID: 25790118] (Flow Cytometry, Human)
• Le Brocq M, Fraser A, Cotton G, Woznica K, McCulloch C, Hewit K, McKimmie C, Nibbs R, Campbell J, Graham G Chemokines as novel and versatile reagents for flow cytometry and cell sorting. J Immunol, 2014-05-21;192(12):6120-30. 2014-05-21 [PMID: 24850722] (Flow Cytometry, Mouse)
• Aruga , Atsushi, Takeshita , Nobuhiro, Kotera , Yoshihit, Okuyama , Ryuji, Matsushita , Norimasa, Ohta , Takehiro, Takeda , Kazuyosh, Yamamoto , Masakazu Phase I clinical trial of multiple-peptide vaccination for patients with advanced biliary tract cancer. J Transl Med, 2014-03-07;12(0):61. 2014-03-07 [PMID: 24606884] (Flow Cytometry, Human)
• Morandi , Fabio, Di Carlo , Emma, Ferrone , Soldano, Petretto , Andrea, Pistoia , Vito, Airoldi , Irma IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation. J Immunol, 2014-02-19;192(6):2634-42. 2014-02-19 [PMID: 24554774] (Flow Cytometry, Human)
• Chiara Vasco, Alessandra Canazza, Ambra Rizzo, Adele Mossa, Elena Corsini, Antonio Silvani et al. Circulating T regulatory cells migration and phenotype in glioblastoma patients: an in vitro study Journal of Neuro-Oncology 2013-12-01 [PMID: 24005771] (Flow Cytometry, Human)
• Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver. J. Immunol., 2010-02-17;184(6):2886-98. 2010-02-17 [PMID: 20164417] (Flow Cytometry, Human)
• Carbone T, Nasorri F, Pennino D CD56highCD16-CD62L- NK cells accumulate in allergic contact dermatitis and contribute to the expression of allergic responses. J. Immunol., 2009-12-11;184(2):1102-10. 2009-12-11 [PMID: 20008290] (Flow Cytometry, Human)
• Barroso-Gonzalez J, El Jaber-Vazdekis N, Garcia-Exposito L, Machado JD, Zarate R, Ravelo AG, Estevez-Braun A, Valenzuela-Fernandez A The Lupane-type Triterpene 30-Oxo-calenduladiol Is a CCR5 Antagonist with Anti-HIV-1 and Anti-chemotactic Activities. J. Biol. Chem., 2009-04-22;284(24):16609-20. 2009-04-22 [PMID: 19386595] (Flow Cytometry, Human)
• Eyerich K, Pennino D, Scarponi C, Foerster S, Nasorri F, Behrendt H, Ring J, Traidl-Hoffmann C, Albanesi C, Cavani A IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response. J. Allergy Clin. Immunol., 2008-12-03;123(1):59-66.e4. 2008-12-03 [PMID: 19056110] (Flow Cytometry, Human)
• Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases. J. Immunol., 2008-12-01;181(11):8053-67. 2008-12-01 [PMID: 19017998] (Flow Cytometry, Human)
• Muller A, Sonkoly E, Eulert C, Gerber PA, Kubitza R, Schirlau K, Franken-Kunkel P, Poremba C, Snyderman C, Klotz LO, Ruzicka T, Bier H, Zlotnik A, Whiteside TL, Homey B, Hoffmann TK Chemokine receptors in head and neck cancer: association with metastatic spread and regulation during chemotherapy. Int. J. Cancer, 2006-05-01;118(9):2147-57. 2006-05-01 [PMID: 16331601] (Flow Cytometry, Human)
• Piovan E, Tosello V, Indraccolo S, Cabrelle A, Baesso I, Trentin L, Zamarchi R, Tamamura H, Fujii N, Semenzato G, Chieco-Bianchi L, Amadori A Chemokine receptor expression in EBV-associated lymphoproliferation in hu/SCID mice: implications for CXCL12/CXCR4 axis in lymphoma generation. Blood, 2004-09-28;105(3):931-9. 2004-09-28 [PMID: 15454484] (Flow Cytometry, Human)
• Casazza S, Zappia E, Pistorio A, Gambini C Recapitulation of B cell differentiation in the central nervous system of patients with multiple sclerosis. Proc. Natl. Acad. Sci. U.S.A., 2004-07-19;101(30):11064-9. 2004-07-19 [PMID: 15263096] (Flow Cytometry, Human)
• Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS. J. Leukoc. Biol., 2003-05-01;73(5):584-90. 2003-05-01 [PMID: 12714572] (Flow Cytometry, Human)

Reviews for CCR4 Antibody (FAB1567P) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer Flow Human 01/21/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID: 24005771
• Species: Human

reviewed by: Verified Customer Flow Human 01/21/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID: 24005771
• Species: Human

Bioinformatics

• Gene Symbol: CCR4
• Uniprot:
  • P51679()