CCR1 Antibody - BSA Free

Western Blot: CCR1 Antibody [NB100-56334] - A375 lysate probed with this antibody at 2 ug/ml dilution.

Flow (Intracellular): CCR1 Antibody [NB100-56334] - An intracellular stain was performed on Raw264.7 cells with CCR1 Antibody NB100-56334 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Flow Cytometry: CCR1 Antibody [NB100-56334] - Image 1: Surface staining of mouse primary epidermal cells (B-6 mice, 30wks) with CL 11-PE, CD11c PE-Cy5, and this antibody with CY5 goat anti rabbit secondary. Image 2: CD11c+ CL II+ cells (Langerans), stained with rabbit isotype control (shaded histogram) were analyzed for CCR1 at 5 ul (blue histogram) and 10 ul (green histogram). Data kindly provided by Corey Parlet, University of Iowa.

Flow (Intracellular): CCR1 Antibody [NB100-56334] - An intracellular stain was performed on U-87 cells with CCR1 Antibody NB100-56334 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Analysis of a FFPE tissue section of human spleen using 1:200 dilution of CCR1 antibody (NB100-56334). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

Analysis of a FFPE tissue section of mouse spleen using 1:200 dilution of CCR1 (NB100-56334) antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

• Reactivity: Hu, Mu, Rb
• Applications: WB, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

CCR1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CCR1 Antibody - BSA Free (NB100-56334) is a polyclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Anti-CCR1 Antibody: Cited in 7 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was raised against a synthetic peptide of human CCR1 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CCR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular) reported in scientific literature (PMID 20371596)
  • Flow Cytometry 5-10 ul/test
  • Immunocytochemistry/ Immunofluorescence 1 ug/ml
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen reported in scientific literature (PMID 15860797)
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1-3 ug/ml
• Application Notes: In A375, a 42 kDa band should be observed.
• Control:

  CCR1 Overexpression Lysate

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 20371596).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CCR1 Antibody - BSA Free

• C-C chemokine receptor type 1
• CC-CKR-1
• CCR1
• CCR-1
• CD191 antigen
• CD191
• chemokine (C-C motif) receptor 1
• CKR1
• CKR-1
• CMKBR1SCYAR1
• CMKR1
• HM145C-C CKR-1
• LD78 receptor
• Macrophage inflammatory protein 1-alpha receptor
• MIP-1alpha-R
• MIP1aR
• RANTES receptor
• RANTES-R

Background

Chemokines play important roles in inflammation and critical for the recruitment of effector immune cells to sites of infection. Chemokines activate leukocytes by binding to G protein coupled receptors (1). The ever-growing chemokine receptor subtypes can be divided into 2 major groups, CXCR and CCR, based on the 2 major classes of chemokines. One of the CCR receptors, CCR1, is expressed on neutrophils, monocytes, lymphocytes, and eosinophils and binds the leukocyte chemoattractant and hemopoiesis regulator macrophage-inflammatory protein (MIP-1), eotaxin, as well as several other related chemokines (2-4). Mice lacking the chemokine receptor CCR1 have defects in neutrophil trafficking and proliferation (5,6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR1 Antibody (NB100-56334)(8)

Publications using NB100-56334

• Messex J, Adams K, Hawkins W et al. Oncogenic Kras-Mediated Cytokine CCL15 Regulates Pancreatic Cancer Cell Migration and Invasion through ROS Cancers 2022-04-26 [PMID: 35565279] (IHC-P, Human)
• Soehnlein Oliver, Drechsler Maik, Doring Yvonne et al. Distinct functions of chemokine receptor axes in the atherogenic mobilization and recruitment of classical monocytes. EMBO Mol Med. 2013-03-01 [PMID: 23417922] (ICC/IF, Mouse)
• Madi A, Sheinin R, Salomon K et al. interFLOW: maximum flow framework for the identification of factors mediating the signaling convergence of multiple receptors Research Square 2023-10-31 (IHC, Mouse)
• Shin SY, Lee DH, Lee J et al. C-C motif chemokine receptor 1 (CCR1) is a target of the EGF-AKT-mTOR-STAT3 signaling axis in breast cancer cells. Oncotarget 2017-11-07 [PMID: 29212252] (IF/IHC, Human)
• Parlet CP, Schlueter AJ. Mechanisms by Which Chronic Ethanol Feeding Impairs the Migratory Capacity of Cutaneous Dendritic Cells. Alcohol Clin Exp Res 2013-12-01 [PMID: 23895590] (FLOW, Mouse)
• Vesosky B, Rottinghaus EK, Stromberg P et al. CCL5 participates in early protection against Mycobacterium tuberculosis. J Leukoc Biol. 2010-06-01 [PMID: 20371596] (Flow Cytometry Control, Flow - IC, Rabbit)
• Choi SW, Hildebrandt GC, Olkiewicz KM et al. CCR1/CCL5 (RANTES) receptor-ligand interactions modulate allogeneic T-cell responses and graft-versus-host disease following stem-cell transplantation. Blood. 2007-11-01 [PMID: 17641205] (Flow-CS)
  
  Details:
  Flow (Cell Surface): Fig 1C (splenocytes harvested from naive donor mice and transplant recipient mice). Splenic CD4+ and CD8+ T-cell expression of CCR1 was analyzed.
• Demoule A, Divangahi M, Danialou G et al. Expression and regulation of CC class chemokines in the dystrophic (mdx) diaphragm. Am J Respir Cell Mol Biol. 2005-08-01 [PMID: 15860797] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: CCR1
• Entrez:
  • 1230(Human)
  • 12768(Mouse)
• Uniprot:
  • NP_001286.1(Human)