CXCR2/IL-8RB Antibody (48311) [Unconjugated]

Images

 
Human peripheral blood granulocytes were stained with Mouse Anti-HumanCXCR2/IL‑8 RB Monoclonal Antibody (Catalog # MAB331, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), ...read more
CXCR2/IL‑8 RB was detected in immersion fixed paraffin-embedded sections of human lymph node using 15 µg/mL Mouse Anti-Human CXCR2/IL‑8 RB Monoclonal Antibody (Catalog # MAB331) overnight at 4 °C. Tissue was ...read more
Recombinant Human CXCL1/GRO alpha (Catalog #275-GR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, B/N, CyTOF-ready
Clone
48311
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

CXCR2/IL-8RB Antibody (48311) [Unconjugated] Summary

Additional Information
Clone 48311 was used by HLDA to establish CD designation
Immunogen
NS0 mouse myeloma cell line transfected with human CXCR2/IL-8 RB
Met1-Leu355
Accession # AAB25880
Specificity
Detects human CXCR2/IL-8 RB transfected NS0 cells but not the parental cell line. It does not cross-react with CXCR1 (IL-8 RA).
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CXCR2
Purity Statement
Protein A or G purified from ascites
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • Neutralization 1-5 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
MAB331 in the following applications:

Publications
Read Publications using
MAB331 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR2/IL-8RB Antibody (48311) [Unconjugated]

  • CD182 antigen
  • CD182
  • CDw128b
  • chemokine (C-X-C motif) receptor 2
  • chemokine (CXC) receptor 2
  • CMKAR2
  • C-X-C chemokine receptor type 2
  • CXCR2 gene for IL8 receptor type B
  • CXCR2
  • CXC-R2
  • CXCR-2
  • GRO/MGSA receptor
  • High affinity interleukin-8 receptor B
  • IL-8 RB
  • IL-8 receptor type 2
  • IL-8R B
  • IL8R2
  • IL8RA
  • IL8RB
  • interleukin 8 receptor B
  • interleukin 8 receptor type 2
  • interleukin-8 receptor type B

Background

CXCR2, also known as IL-8 RB, is a G protein-coupled chemokine receptor expressed on neutrophils. It binds IL-8, GRO alpha , GRO beta , GRO gamma , NAP-2, and ENA-78.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CXCR2/IL-8RB Antibody (MAB331)(54)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Guinea Pig, Rabbit.

We have publications tested in 10 applications: Bioassay, ELISA Development, Flow Cytometry, Functional Assay, ICC, IHC, IHC-Fr, IHC-P, Neutralization, Western Blot.


Filter By Application
Bioassay
(1)
ELISA Development
(1)
Flow Cytometry
(13)
Functional Assay
(1)
ICC
(3)
IHC
(7)
IHC-Fr
(3)
IHC-P
(7)
Neutralization
(23)
Western Blot
(6)
All Applications
Filter By Species
Human
(48)
Mouse
(4)
Rat
(1)
Guinea Pig
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 54. Show All 54 Publications.
Publications using MAB331 Applications Species
Zhao, C;Wang, D;Li, Z;Zhang, Z;Xu, Y;Liu, J;Lei, Q;Han, D;Huo, Y;Liu, S;Li, L;Zhang, Y; IL8 derived from macrophages inhibits CD8+ T-cell function by downregulating TIM3 expression through IL8-CXCR2 axis in patients with advanced colorectal cancer International immunopharmacology 2023-06-16 [PMID: 37331296] (Neutralization, Human) Neutralization Human
R Tanaka, K Kimura, S Eguchi, G Ohira, S Tanaka, R Amano, H Tanaka, M Yashiro, M Ohira, S Kubo Interleukin-8 produced from cancer-associated fibroblasts suppresses proliferation of the OCUCh-LM1 cancer cell line BMC Cancer, 2022-07-08;22(1):748. 2022-07-08 [PMID: 35804329] (Neutralization, Western Blot, Human) Neutralization, Western Blot Human
G Tsujio, K Maruo, Y Yamamoto, T Sera, A Sugimoto, H Kasashima, Y Miki, M Yoshii, T Tamura, T Toyokawa, H Tanaka, K Muguruma, M Ohira, M Yashiro Significance of tumor heterogeneity of p-Smad2 and c-Met in HER2-positive gastric carcinoma with lymph node metastasis BMC Cancer, 2022-06-01;22(1):598. 2022-06-01 [PMID: 35650563] (IHC, Human) IHC Human
Y Yamamoto, A Sugimoto, K Maruo, G Tsujio, T Sera, S Kushiyama, S Nishimura, K Kuroda, S Togano, S Eguchi, R Tanaka, K Kimura, R Amano, M Ohira, M Yashiro CXCR2 signaling might have a tumor-suppressive role in patients with cholangiocarcinoma PLoS ONE, 2022-04-04;17(4):e0266027. 2022-04-04 [PMID: 35377900] (IHC, Western Blot, Human) IHC, Western Blot Human
N Freitag, DM Baston-Bue, JS Kruessel, UR Markert, TN Fehm, AP Bielfeld Eutopic endometrial immune profile of infertility-patients with and without endometriosis Journal of reproductive immunology, 2022-01-29;150(0):103489. 2022-01-29 [PMID: 35149274] (IHC, Human) IHC Human
S Ali, TF Borin, R Piranliogl, R Ara, I Lebedyeva, K Angara, BR Achyut, AS Arbab, MH Rashid Changes in the tumor microenvironment and outcome for TME-targeting therapy in glioblastoma: A pilot study PLoS ONE, 2021-02-05;16(2):e0246646. 2021-02-05 [PMID: 33544755] (Flow Cytometry, IHC, Mouse) Flow Cytometry, IHC Mouse
AJ Hoogendijk, F Pourfarzad, CEM Aarts, ATJ Tool, IH Hiemstra, L Grassi, M Frontini, AB Meijer, M van den Bi, TW Kuijpers Dynamic Transcriptome-Proteome Correlation Networks Reveal Human Myeloid Differentiation and Neutrophil-Specific Programming Cell Rep, 2019-11-19;29(8):2505-2519.e4. 2019-11-19 [PMID: 31747616] (Flow Cytometry, Human) Flow Cytometry Human
F Caccuri, A Bugatti, S Corbellini, S Roversi, A Zani, P Mazzuca, S Marsico, A Caruso, C Giagulli The Synthetic Dipeptide Pidotimod Shows a Chemokine-Like Activity through CXC Chemokine Receptor 3 (CXCR3) Int J Mol Sci, 2019-10-24;20(21):. 2019-10-24 [PMID: 31653015] (Neutralization, Human) Neutralization Human
HK Kim, SG Lee, SW Lee, BJ Oh, JH Kim, JA Kim, G Lee, JD Jang, YA Joe A Subset of Paracrine Factors as Efficient Biomarkers for Predicting Vascular Regenerative Efficacy of Mesenchymal Stromal/Stem Cells Stem Cells, 2018-10-08;0(0):. 2018-10-08 [PMID: 30281870] (Neutralization, Human) Neutralization Human
K Angara, TF Borin, MH Rashid, I Lebedyeva, R Ara, PC Lin, A Iskander, RJ Bollag, BR Achyut, AS Arbab CXCR2-Expressing Tumor Cells Drive Vascular Mimicry in Antiangiogenic Therapy-Resistant Glioblastoma Neoplasia, 2018-10-01;20(10):1070-1082. 2018-10-01 [PMID: 30236892] (ICC, Human) ICC Human
Show All 54 Publications.

Reviews for CXCR2/IL-8RB Antibody (MAB331) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using MAB331:
Filter by Applications
IHC-P
(2)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
IHC-P Human 02/20/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 22763101
SpeciesHuman
  4
reviewed by:
Verified Customer
IHC-P Human 02/20/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 22626766
SpeciesHuman

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FAQs for CXCR2/IL-8RB Antibody (MAB331) (0)

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Secondary Antibodies

 

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

 
Verified Customer
02/20/2015
Application: IHC-P
Species: Human

 
Verified Customer
02/20/2015
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol CXCR2
Uniprot