CCR1 Overexpression Lysate (Native)

Images

 
Western Blot: CCR1 Overexpression Lysate (Adult Normal) [NBL1-08887] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CCR1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CCR1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC208605 and based on accession number NM_001295. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens chemokine (C-C motif) receptor 1 (CCR1), mRNA.
Gene
CCR1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for CCR1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CCR1 Overexpression Lysate (Native)

  • C-C chemokine receptor type 1
  • CC-CKR-1
  • CCR1
  • CCR-1
  • CD191 antigen
  • CD191
  • chemokine (C-C motif) receptor 1
  • CKR1
  • CKR-1
  • CMKBR1SCYAR1
  • CMKR1
  • HM145C-C CKR-1
  • LD78 receptor
  • Macrophage inflammatory protein 1-alpha receptor
  • MIP-1alpha-R
  • MIP1aR
  • RANTES receptor
  • RANTES-R

Background

This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The ligands of this receptor include macrophage inflammatory protein 1 alpha (MIP-1 alpha), regulated on activation normal T expressed and secreted protein (RANTES), monocyte chemoattractant protein 3 (MCP-3), and myeloid progenitor inhibitory factor-1 (MPIF-1). Chemokines and their receptors mediated signal transduction are critical for the recruitment of effector immune cells to the site of inflammation. Knockout studies of the mouse homolog suggested the roles of this gene in host protection from inflammatory response, and susceptibility to virus and parasite. This gene and other chemokine receptor genes, including CCR2, CCRL2, CCR3, CCR5 and CCXCR1, are found to form a gene cluster on chromosome 3p. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for CCR1 Lysate (NBL1-08887) (0)

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Blogs on CCR1.

CCR1 (C-C chemokine receptor type 1)
Chemokines play a central role in inflammation and are crucial for recruitment of immune cells to sites of infection. The chemokine-dependent activation of leukocytes occurs through binding to G-protein coupled receptors. These chemokine receptor s...  Read full blog post.

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Bioinformatics

Gene Symbol CCR1
Entrez