FAB3832A) and either (A) Mouse Anti-Human CCR1 Monoclonal Antibody (Catalog # MAB145) or (B) isotype control antibody (Catalog # MAB004) followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0116). View our protocol for Staining Membrane-associated Proteins. " alt="PBMC Monocytes were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human CCR1 Monoclonal Antibody (Catalog # MAB145) or (B) isotype control antibody (Catalog # MAB004) followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0116). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56334SS | |||
HAF005). A specific band was detected for CCR1 at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse heart tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse CCR1 Monoclonal Antibody (Catalog # MAB5986) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CCR1 at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
HAF016). A specific band was detected for CCR1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of mouse heart tissue and mouse spleen tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse CCR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5986) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CCR1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5986-SP | |||
FAB3832A) and either (A) Mouse Anti-Human CCR1 Monoclonal Antibody (Catalog # MAB145R) or (B) Mouse IgG2B Isotype Control (Catalog # MAB0041) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." alt="Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human CCR1 Monoclonal Antibody (Catalog # MAB145R) or (B) Mouse IgG2B Isotype Control (Catalog # MAB0041) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB145R-SP | |||
| Carrier Free | FAB145RV-100UG | |||
| Carrier Free | FAB145RU-100UG | |||
| Carrier Free | FAB145RT-100UG | |||
| Carrier Free | FAB145RS-100UG | |||
| Carrier Free | FAB145RR-100UG | |||
| Carrier Free | FAB145RN-100UG | |||
| Carrier Free | FAB145RG-100UG | |||
MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins." alt="RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse CCR1 Monoclonal Antibody (Catalog # MAB5986R, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB5986R-SP | |||
| Carrier Free | FAB5986RV-100UG | |||
| Carrier Free | FAB5986RU-100UG | |||
| Carrier Free | FAB5986RT-100UG | |||
| Carrier Free | FAB5986RS-100UG | |||
| Carrier Free | FAB5986RR-100UG | |||
| Carrier Free | FAB5986RN-100UG | |||
| Carrier Free | FAB5986RG-100UG | |||
F0101B). View our protocol for Staining Membrane-associated Proteins." alt="NS0 mouse myeloma cell line transfected with Human CCR1and eGFP was stained with Mouse Anti-Human CCR1 Monoclonal Antibody (Catalog # MAB1451, filled histogram) or Mouse IgG2A Isotype Control Antibody (Catalog # MAB033, open histogram) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1451-SP | |||
| Carrier Free | FAB1451V-100UG | |||
| Carrier Free | FAB1451U-100UG | |||
| Carrier Free | FAB1451T-100UG | |||
| Carrier Free | FAB1451S-100UG | |||
| Carrier Free | FAB1451R-100UG | |||
| Carrier Free | FAB1451N-100UG | |||
| Carrier Free | FAB1451G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61697-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89234-50ul | |||
![Western Blot: CCR1 Antibody [NB100-56334] - A375 lysate probed with this antibody at 2 ug/ml dilution.](http://images.novusbio.com/fullsize/CCR1-Antibody-Western-Blot-NB100-56334-img0002.jpg "Western Blot: CCR1 Antibody [NB100-56334] - A375 lysate probed with this antibody at 2 ug/ml dilution.")
![Flow (Intracellular): CCR1 Antibody [NB100-56334] - An intracellular stain was performed on Raw264.7 cells with CCR1 Antibody NB100-56334 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/CCR1-Antibody-Flow-Intracellular-NB100-56334-img0008.jpg)
![Flow Cytometry: CCR1 Antibody [NB100-56334] - Image 1: Surface staining of mouse primary epidermal cells (B-6 mice, 30wks) with CL 11-PE, CD11c PE-Cy5, and this antibody with CY5 goat anti rabbit secondary. Image 2: CD11c+ CL II+ cells (Langerans), stained with rabbit isotype control (shaded histogram) were analyzed for CCR1 at 5 ul (blue histogram) and 10 ul (green histogram). Data kindly provided by Corey Parlet, University of Iowa.](http://images.novusbio.com/fullsize/CCR1-Antibody-Flow-Cytometry-NB100-56334-img0004.jpg)
![Flow (Intracellular): CCR1 Antibody [NB100-56334] - An intracellular stain was performed on U-87 cells with CCR1 Antibody NB100-56334 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/CCR1-Antibody-Flow-Intracellular-NB100-56334-img0007.jpg)
MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # ![Western Blot: CCR1 Antibody [NBP1-78173] - Mouse Lung samples. 1:500 antibody dilution in DiluObuffer. Apparent MW is 44KDa.](http://images.novusbio.com/fullsize/CCR1-Antibody-Western-Blot-NBP1-78173-img0002.jpg "Western Blot: CCR1 Antibody [NBP1-78173] - Mouse Lung samples. 1:500 antibody dilution in DiluObuffer. Apparent MW is 44KDa.")
![Immunohistochemistry-Paraffin: CCR1 Antibody [NBP1-78173] - Mouse Lung. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X manginification. FFPE section.](http://images.novusbio.com/fullsize/CCR1-Antibody-Immunohistochemistry-Paraffin-NBP1-78173-img0004.jpg)
![Immunohistochemistry-Paraffin: CCR1 Antibody [NBP1-78173] - Mouse Spleen. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X mangification. FFPE secction.](http://images.novusbio.com/fullsize/CCR1-Antibody-Immunohistochemistry-Paraffin-NBP1-78173-img0003.jpg)
![Western Blot: CCR1 Antibody (8E10G6) [NBP2-61697] - Analysis using CD191 mAb against human CD191 (AA: extra mix) recombinant protein. (Expected MW is 37 kDa)](http://images.novusbio.com/fullsize/CCR1-Antibody-8E10G6-Western-Blot-NBP2-61697-img0003.jpg "Western Blot: CCR1 Antibody (8E10G6) [NBP2-61697] - Analysis using CD191 mAb against human CD191 (AA: extra mix) recombinant protein. (Expected MW is 37 kDa)")
![Flow Cytometry: CCR1 Antibody (8E10G6) [NBP2-61697] - Analysis of Ramos cells using CD191 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/CCR1-Antibody-8E10G6-Flow-Cytometry-NBP2-61697-img0002.jpg)
![Western Blot: CCR1 Antibody (8E10G6) [NBP2-61697] - Analysis using CD191 mAb against HEK293 (1) and CD191 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CCR1-Antibody-8E10G6-Western-Blot-NBP2-61697-img0004.jpg)
![ELISA: CCR1 Antibody (8E10G6) [NBP2-61697] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/CCR1-Antibody-8E10G6-ELISA-NBP2-61697-img0001.jpg)
![Immunohistochemistry-Paraffin: CCR1 Antibody [NLS1242] - Anti-CCR1 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/CCR1-Antibody-Immunohistochemistry-Paraffin-NLS1242-img0005.jpg "Immunohistochemistry-Paraffin: CCR1 Antibody [NLS1242] - Anti-CCR1 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: CCR1 Antibody [NLS1242] - Analysis of anti-CCR1 antibody with human spleen, lymphocytes.](http://images.novusbio.com/fullsize/CCR1-Antibody-Immunohistochemistry-Paraffin-NLS1242-img0001.jpg)
![Immunohistochemistry-Paraffin: CCR1 Antibody [NLS1242] - Analysis of anti-CCR1 antibody with human colon, carcinoma.](http://images.novusbio.com/fullsize/CCR1-Antibody-Immunohistochemistry-Paraffin-NLS1242-img0003.jpg)
![Immunohistochemistry-Paraffin: CCR1 Antibody [NLS1242] - Anti-CCR1 antibody IHC of human Lung, Adenocarcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/CCR1-Antibody-Immunohistochemistry-Paraffin-NLS1242-img0004.jpg)
![Western Blot: CCR1 Antibody (002) [NBP2-89234] - Lane A: HEK293 Whole Cell Lysate Lane B: 293T Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:43 kDa Observed band size:49 kDa](http://images.novusbio.com/fullsize/CCR1-Antibody-002-Western-Blot-NBP2-89234-img0001.jpg "Western Blot: CCR1 Antibody (002) [NBP2-89234] - Lane A: HEK293 Whole Cell Lysate Lane B: 293T Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:43 kDa Observed band size:49 kDa")
![Immunoprecipitation: CCR1 Antibody (002) [NBP2-89234] - Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg 293T Whole Cell Lysate2 uL anti-CCR1/CD191 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CCR1/CD191 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 43 kDa Observed band size: 43 kDa](http://images.novusbio.com/fullsize/CCR1-Antibody-002-Immunoprecipitation-NBP2-89234-img0002.jpg)
