CXCR1/IL-8RA Antibody (42705) [Unconjugated]

Human peripheral blood granulocytes were stained with Mouse Anti-HumanCXCR1/IL‑8 RA Monoclonal Antibody (Catalog # MAB330, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

CXCR1/IL‑8 RA was detected in immersion fixed paraffin-embedded sections of human endometrial cancer tissue using Mouse Anti-Human CXCR1/IL‑8 RA Monoclonal Antibody (Catalog # MAB330) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

CXCR1/IL‑8 RA was detected in immersion fixed frozen sections of human lymph nodes using Mouse Anti-Human CXCR1/IL‑8 RA Monoclonal Antibody (Catalog # MAB330) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to lymphocytes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Recombinant Human CXCL8/IL‑8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL8/IL‑8 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR1/IL‑8 RA Monoclonal Antibody (Catalog # MAB330). The ND50 is typically 0.4‑2.0 µg/mL.

• Reactivity: Hu
• Applications: Flow, IHC, B/N, CyTOF-reported
• Clone: 42705
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CXCR1/IL-8RA Antibody (42705) [Unconjugated] Summary

• Additional Information: Clone 42705 was used by HLDA to establish CD designation
• Immunogen: NS0 mouse myeloma cell line transfected with human CXCR1/IL-8 RA
  Met1-Leu350
  Accession # AAA59159
• Specificity: Detects human CXCR1/IL-8 RA transfectants but not the parental cell line. It does not cross-react with human CXCR2.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CXCR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-reported
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • Neutralization 0.4-2.0 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR1/IL-8RA Antibody (42705) [Unconjugated]

• CD128
• CD181 antigen
• CD181
• CDw128aC-C
• chemokine (C-X-C motif) receptor 1
• CKR-1
• CMKAR1
• C-X-C chemokine receptor type 1
• CXCR1
• CXC-R1
• CXCR-1
• High affinity interleukin-8 receptor A
• IL-8 RA
• IL-8 receptor type 1
• IL-8R A
• IL8R1
• IL8RA
• IL8RAC-C-CKR-1
• IL8RBA
• interleukin 8 receptor, alpha
• interleukin-8 receptor type 1
• interleukin-8 receptor type A

Background

The human C-X-C chemokine IL-8 is a potent neutrophil chemotactic and activating factor. Two distinct G protein-linked cell surface receptors, known as IL-8 RA (type I or CXCR1) and IL-8 RB (type II or CXCR2), can interact with the IL-8 molecule. These two receptors share 77% amino acid homology. CXCR1 expression has been documented on neutrophils, monocytes, and a small population of T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR1/IL-8RA Antibody (MAB330)(39)

Publications using MAB330

• SK Sarvestani, S Signs, B Hu, Y Yeu, H Feng, Y Ni, DR Hill, RC Fisher, S Ferrandon, RK DeHaan, J Stiene, M Cruise, TH Hwang, X Shen, JR Spence, EH Huang Induced organoids derived from patients with ulcerative colitis recapitulate colitic reactivity Nature Communications, 2021;12(1):262. 2021-01-01 [PMID: 33431859] (IHC, Human)
• PC Hart, HA Kenny, N Grassl, KM Watters, LM Litchfield, F Coscia, I Blaženovi?, L Ploetzky, O Fiehn, M Mann, E Lengyel, IL Romero Mesothelial Cell HIF1alpha Expression Is Metabolically Downregulated by Metformin to Prevent Oncogenic Tumor-Stromal Crosstalk Cell Rep, 2019;29(12):4086-4098.e6. 2019-01-01 [PMID: 31851935] (Neutralization, Mouse)
• AJ Hoogendijk, F Pourfarzad, CEM Aarts, ATJ Tool, IH Hiemstra, L Grassi, M Frontini, AB Meijer, M van den Bi, TW Kuijpers Dynamic Transcriptome-Proteome Correlation Networks Reveal Human Myeloid Differentiation and Neutrophil-Specific Programming Cell Rep, 2019;29(8):2505-2519.e4. 2019-01-01 [PMID: 31747616] (Flow Cytometry, Human)
• F Caccuri, A Bugatti, S Corbellini, S Roversi, A Zani, P Mazzuca, S Marsico, A Caruso, C Giagulli The Synthetic Dipeptide Pidotimod Shows a Chemokine-Like Activity through CXC Chemokine Receptor 3 (CXCR3) Int J Mol Sci, 2019;20(21):. 2019-01-01 [PMID: 31653015] (Neutralization, Human)
• I Sharma, A Singh, F Siraj, S Saxena IL-8/CXCR1/2 signalling promotes tumor cell proliferation, invasion and vascular mimicry in glioblastoma J. Biomed. Sci., 2018;25(1):62. 2018-01-01 [PMID: 30086759] (Bioassay, Human)
• CH Hung, FM Chen, YC Lin, ML Tsai, SL Wang, YC Chen, YT Chen, MF Hou Altered monocyte differentiation and macrophage polarization patterns in patients with breast cancer BMC Cancer, 2018;18(1):366. 2018-01-01 [PMID: 29614988] (Flow Cytometry, Human)
• P Mazzuca, S Marsico, K Schulze, S Mitola, MC Pils, C Giagulli, CA Guzman, A Caruso, F Caccuri Role of autophagy in the HIV-1 matrix protein p17-driven lymphangiogenesis J. Virol., 2017;0(0):. 2017-01-01 [PMID: 28592537] (Neutralization, Human)
• ALT Loi, S Hoonhorst, C van Aalst, J Langereis, V Kamp, S Sluis-Eisi, N Ten Hacken, JW Lammers, L Koenderman Proteomic profiling of peripheral blood neutrophils identifies two inflammatory phenotypes in stable COPD patients Respir. Res., 2017;18(1):100. 2017-01-01 [PMID: 28532454] (Flow Cytometry, Human)
• Nader Alaridah Mycobacteria Manipulate G-Protein-Coupled Receptors to Increase Mucosal Rac1 Expression in the Lungs J Innate Immun, 2016;0(0):. 2016-01-01 [PMID: 28013312] (Neutralization, Mouse)
• Chen , Lianyu, Fan , Jie, Chen , Hao, Meng , Zhiqiang, Chen , Zhen, Wang , Peng, Liu , Luming The IL-8/CXCR1 axis is associated with cancer stem cell-like properties and correlates with clinical prognosis in human pancreatic cancer cases. Sci Rep, 2014;4(0):5911. 2014-01-01 [PMID: 25081383] (Neutralization, Human)
• Al-Alwan L, Chang Y, Rousseau S, Martin J, Eidelman D, Hamid Q CXCL1 inhibits airway smooth muscle cell migration through the decoy receptor Duffy antigen receptor for chemokines. J Immunol, 2015;193(3):1416-26. 2015-01-01 [PMID: 24981451] (Western Blot, Human)
• Chen , Lianyu, Qu , Chao, Chen , Hao, Xu , Litao, Qi , Qi, Luo , Jianmin, Wang , Kun, Meng , Zhiqiang, Chen , Zhen, Wang , Peng, Liu , Luming Chinese herbal medicine suppresses invasion-promoting capacity of cancer-associated fibroblasts in pancreatic cancer. PLoS ONE, 2014;9(4):e96177. 2014-01-01 [PMID: 24781445] (Neutralization, Human)
• Cartwright A, Schmutz C, Askari A, Kuiper J, Middleton J Orphan receptor GPR15/BOB is up-regulated in rheumatoid arthritis. Cytokine, 2014;67(2):53-9. 2014-01-01 [PMID: 24725539] (Flow Cytometry, Human)
• Caccuri , Francesc, Giagulli , Cinzia, Reichelt , Joachim, Martorelli , Debora, Marsico , Stefania, Bugatti , Antonell, Barone , Ines, Rusnati , Marco, Guzman , Carlos A, Dolcetti , Riccardo, Caruso , Arnaldo Simian immunodeficiency virus and human immunodeficiency virus type 1 matrix proteins specify different capabilities to modulate B cell growth. J Virol, 2014;88(10):5706-17. 2014-01-01 [PMID: 24623414] (Neutralization, Human)
• Omatsu T, Cepinskas G, Clarson C, Patterson E, Alharfi I, Summers K, Couraud P, Romero I, Weksler B, Fraser D CXCL1/CXCL8 (GROalpha/IL-8) in human diabetic ketoacidosis plasma facilitates leukocyte recruitment to cerebrovascular endothelium in vitro. Am J Physiol Endocrinol Metab, 2014;306(9):E1077-84. 2014-01-01 [PMID: 24619879] (Neutralization, Human)
• Liu L, Xie F, Chang K, Li M, Meng Y, Wang X, Li H, Li D, Yu J Hypoxia promotes the proliferation of cervical carcinoma cells through stimulating the secretion of IL-8. Int J Clin Exp Pathol, 0;7(2):575-83. [PMID: 24551277] (IHC-P, Human)
• Chen C, Chen L, Hsu Y, Liu K, Fan C, Huang T The endothelin-integrin axis is involved in macrophage-induced breast cancer cell chemotactic interactions with endothelial cells. J Biol Chem, 2014;289(14):10029-44. 2014-01-01 [PMID: 24550382] (Neutralization, Human)
• Shi G, Han J, Liu G, Hao Y, Ma Y, Li T, Wu X, Zhang H, Liu Y, Wang B, Kong Y, Zhou J, Zeng H Expansion of activated regulatory T cells by myeloid-specific chemokines via an alternative pathway in CSF of bacterial meningitis patients. Eur J Immunol, 2014;44(2):420-30. 2014-01-01 [PMID: 24155161] (Neutralization, Human)
• Infanger , David W, Cho , YouJin, Lopez , Brina S, Mohanan , Sunish, Liu , S Chris, Gursel , Demirkan, Boockvar , John A, Fischbach , Claudia Glioblastoma stem cells are regulated by interleukin-8 signaling in a tumoral perivascular niche. Cancer Res, 2013;73(23):7079-89. 2013-01-01 [PMID: 24121485] (Functional Assay, Human)
• Nieman KM, Kenny HA, Penicka CV, Ladanyi A, Buell-Gutbrod R, Zillhardt MR, Romero IL, Carey MS, Mills GB, Hotamisligil GS, Yamada SD, Peter ME, Gwin K, Lengyel E Adipocytes promote ovarian cancer metastasis and provide energy for rapid tumor growth. Nat. Med., 2011;17(11):1498-503. 2011-01-01 [PMID: 22037646] (In Vivo, Human)
• Eikawa S, Ohue Y, Kitaoka K Enrichment of Foxp3+ CD4 regulatory T cells in migrated T cells to IL-6- and IL-8-expressing tumors through predominant induction of CXCR1 by IL-6. J. Immunol., 2010;185(11):6734-40. 2010-01-01 [PMID: 21048114] (Neutralization, Human)
• Weiss N, Deboux C, Chaverot N, Miller F, Baron-Van Evercooren A, Couraud P, Cazaubon S IL8 and CXCL13 are potent chemokines for the recruitment of human neural precursor cells across brain endothelial cells. J Neuroimmunol, 0;223(1):131-4. [PMID: 20400187] (Flow Cytometry, Human)
• Mintz R, Garty BZ, Meshel T, Marcus N, Katanov C, Cohen-Hillel E, Ben-Baruch A Reduced expression of chemoattractant receptors by polymorphonuclear leukocytes in Hyper IgE Syndrome patients. Immunol. Lett., 2010;130(1):97-106. 2010-01-01 [PMID: 20005258] (Flow Cytometry, Human)
• van der Veen BS, Petersen AH, Belperio JA, Satchell SC, Mathieson PW, Molema G, Heeringa P Spatiotemporal expression of chemokines and chemokine receptors in experimental anti-myeloperoxidase antibody-mediated glomerulonephritis. Clin. Exp. Immunol., 2009;158(1):143-53. 2009-01-01 [PMID: 19737241] (Neutralization, Human)
• Singh S, Nannuru KC, Sadanandam A, Varney ML, Singh RK CXCR1 and CXCR2 enhances human melanoma tumourigenesis, growth and invasion. Br. J. Cancer, 2009;100(10):1638-46. 2009-01-01 [PMID: 19401689] (Western Blot, Human)
• Rowe SM, Jackson PL, Liu G, Hardison M, Livraghi A, Solomon GM, McQuaid DB, Noerager BD, Gaggar A, Clancy JP, O'Neal W, Sorscher EJ, Abraham E, Blalock JE Potential role of high-mobility group box 1 in cystic fibrosis airway disease. Am. J. Respir. Crit. Care Med., 2008;178(8):822-31. 2008-01-01 [PMID: 18658107] (Neutralization, Human)
• Goczalik I, Ulbricht E, Hollborn M, Raap M, Uhlmann S, Weick M, Pannicke T, Wiedemann P, Bringmann A, Reichenbach A, Francke M Expression of CXCL8, CXCR1, and CXCR2 in neurons and glial cells of the human and rabbit retina. Invest. Ophthalmol. Vis. Sci., 2008;49(10):4578-89. 2008-01-01 [PMID: 18552386] (IHC-Fr, Human)
• Gaggar A, Jackson PL, Noerager BD, O'Reilly PJ, McQuaid DB, Rowe SM, Clancy JP, Blalock JE A novel proteolytic cascade generates an extracellular matrix-derived chemoattractant in chronic neutrophilic inflammation. J. Immunol., 2008;180(8):5662-9. 2008-01-01 [PMID: 18390751] (Neutralization, Mouse)
• Lee D, King MR Shear-induced capping of L-selectin on the neutrophil surface during centrifugation. J. Immunol. Methods, 2007;328(1):97-105. 2007-01-01 [PMID: 17915247] (ICC, Human)
• Tsai WH, Hsu HC, Lin CC, Ho CK, Kou YR Role of interleukin-8 and growth-regulated oncogene-alpha in the chemotactic migration of all-trans retinoic acid-treated promyelocytic leukemic cells toward alveolar epithelial cells. Crit. Care Med., 2007;35(3):879-85. 2007-01-01 [PMID: 17235257] (Neutralization, Human)
• Smith C, Damas JK, Otterdal K, ØIe E, Sandberg WJ, Yndestad A, Waehre T, Scholz H, Endresen K, Olofsson PS, Halvorsen B, Gullestad L, Froland SS, Hansson GK, Aukrust P Increased levels of neutrophil-activating peptide-2 in acute coronary syndromes: possible role of platelet-mediated vascular inflammation. J. Am. Coll. Cardiol., 2006;48(8):1591-9. 2006-01-01 [PMID: 17045893] (Neutralization, Human)
• Kline M, Donovan K, Wellik L, Lust C, Jin W, Moon-Tasson L, Xiong Y, Witzig TE, Kumar S, Rajkumar SV, Lust JA Cytokine and chemokine profiles in multiple myeloma; significance of stromal interaction and correlation of IL-8 production with disease progression. Leuk. Res., 2007;31(5):591-8. 2007-01-01 [PMID: 16879867] (Flow Cytometry, Human)
• Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines. Clin. Immunol., 2006;120(2):220-31. 2006-01-01 [PMID: 16782408] (Neutralization, Human)
• Haas CS, Martinez RJ, Attia N, Haines GK, Campbell PL, Koch AE Chemokine receptor expression in rat adjuvant-induced arthritis. Arthritis Rheum., 2005;52(12):3718-30. 2005-01-01 [PMID: 16320322] (IHC, Rat)
• Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis. J. Immunol., 2005;175(9):6177-89. 2005-01-01 [PMID: 16237115] (Neutralization, Human)
• Malgorzata Goczalik I, Raap M, Weick M, Milenkovic I, Heidmann J, Enzmann V, Wiedemann P, Reichenbach A, Francke M The activation of IL-8 receptors in cultured guinea pig Muller glial cells is modified by signals from retinal pigment epithelium. J. Neuroimmunol., 2005;161(1):49-60. 2005-01-01 [PMID: 15748943] (ICC, Guinea Pig)
• Doubrovina ES, Doubrovin MM, Vider E, Sisson RB, O&amp;amp;apos;Reilly RJ, Dupont B, Vyas YM Evasion from NK cell immunity by MHC class I chain-related molecules expressing colon adenocarcinoma. J. Immunol., 2003;171(12):6891-9. 2003-01-01 [PMID: 14662896] (Flow Cytometry, Human)
• Annels NE, Willemze AJ, van der Velden VH, Faaij CM, van Wering E, Sie-Go DM, Egeler RM, van Tol MJ, Révész T Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL. Blood, 2003;103(7):2806-8. 2003-01-01 [PMID: 14656893] (Flow Cytometry, Human)
• Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstadt H Expression of functional CCR and CXCR chemokine receptors in podocytes. J. Immunol., 2002;168(12):6244-52. 2002-01-01 [PMID: 12055238] (IHC-Fr, Human)

Bioinformatics

• Gene Symbol: CXCR1
• Uniprot:
  • AAA59159()