Caspase 5 Antibody

Images

 
Western Blot: Caspase 5 Antibody [NBP1-77209] - Analysis of Caspase 5 in Ramos cells with anti-Caspase 5 (IN) using NBP1-77209 at (A) 0.5, (B) 1, and (C) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: Caspase 5 Antibody [NBP1-77209] - P815 cells with Caspase-5 antibody at 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: Caspase 5 Antibody [NBP1-77209] - P815 cells with caspase-5 antibody at 2 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Caspase 5 Antibody Summary

Immunogen
Antibody was raised against a 14 amino acid synthetic peptide from the center of human Caspase-5. The immunogen is located within amino acids 170 - 220 of Caspase-5.
Specificity
Depending on cell lines or tissues used, other cleavage products may be observed.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CASP5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 2 ug/ml
Application Notes
Casp-5 antibody can be used for the detection of caspase-5 by Western blot at 0.5 - 1 mg/mlDepending on cell lines or tissues used, other cleavage products may be observed.
Control Peptide
Caspase 5 Peptide (NBP1-77209PEP)
Publications
Read Publications using NBP1-77209.

Reactivity Notes

Predicted species reactivity based on immunogen sequence: Bovine: (79%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Caspase 5 Antibody

  • CASP5
  • CASP-5
  • caspase 5, apoptosis-related cysteine peptidase
  • caspase 5, apoptosis-related cysteine protease
  • Caspase5
  • Caspase-5
  • EC 3.4.22
  • EC 3.4.22.58
  • ICE(rel)III
  • ICE(rel)-III
  • ICEREL-III
  • ICH3
  • ICH-3
  • MGC141966
  • Protease ICH-3
  • Protease TY
  • TY protease

Background

Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1beta and IL-18. Caspase-5 can interact with caspase-1; both are constituents of the NALP1 inflammasome, a complex that can trigger the cleavage of pro-IL-1beta. Expression of caspase-5 can be regulated by lipopolysaccharide (LPS) and IFN-gamma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Caspase 5 Antibody (NBP1-77209)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Research Areas for Caspase 5 Antibody (NBP1-77209)

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Blogs on Caspase 5.

CARD & NFKB Antibodies for Apoptosis Research
Apoptosis is one of the main types of programmed cell death which involves a cascade of biochemical events leading to specific cell morphology characteristics and ultimately death of cells.Caspases play crucial roles in modulating cellular signaling...  Read full blog post.

Caspase Antibodies as Cancer Biomarkers
We at Novus Biologicals are one of the leading antibody suppliers for products targeted to apoptosis. These products are regularly used by cancer research groups - apoptosis is fundamental to developing therapies that will kill tumor cells. Caspase pr...  Read full blog post.

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Bioinformatics

Gene Symbol CASP5
Uniprot