Western Blot: Caspase-6 Antibody [NB100-56114] - Analysis of Caspase-6 using this antibody. Recombinant catalytically active Caspase-6 was western blotted with Active/Cleaved Caspase-6 antibody. The antibody detected ...read more
Immunohistochemistry-Paraffin: Caspase-6 Antibody [NB100-56114] - Formalin-fixed paraffin-embedded tissue sections of human gliomas stained for Active/Cleaved Caspase-6 expression using Caspase-6 antibody at 1:2000. ...read more
Immunohistochemistry: Caspase-6 Antibody [NB100-56114] - Formalin-fixed paraffin-embedded tissue section of dog cerebellum stained for Caspase-6 expression at 1:2000. Hematoxylin-eosin counterstain.
Recombinant catalytically active human Caspase-6 protein was used as immunogen.
This polyclonal antisera recognizes the large and small subunits of active/cleaved caspase-6. Whereas the antisera has a strong preference for active/cleaved caspase-6, in some cell or tissue systems or techniques the antisera may also recognize the ~34 kDa proform of caspase-6 as well as intermediate caspase-6 cleavage fragments.
Test in a species/application not listed above to receive a full credit towards a future purchase.
Cross reacts with Dog, Gerbil, Human, Mouse and Rat. Not yet tested in other species.
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
50 ul neat whole antisera
0.05% Sodium Azide
Alternate Names for Caspase-6 Antibody
Apoptotic protease Mch-2
caspase 6, apoptosis-related cysteine peptidase
caspase 6, apoptosis-related cysteine protease
Caspases belong to the cysteine-aspartic acid protease (Caspase) family which plays a major role in the transduction of the apoptotic signal and execution of apoptosis in mammalian cells. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein could be processed by caspases 7, 8 and 10, and is thought to function as a downstream. Caspase-3 (CASP3/CPP32) and Caspase-6 (CASP6/Mch2) are the major active caspases in apoptotic cells and are activated in response to distinct apoptosis-inducing stimuli and in all cell, lines analyzed. Both CPP32 and Mch2 are present in apoptotic cells as multiple active species. Mch2 cleaves nuclear mitotic apparatus protein (NuMA) and mediates the shrinkage and fragmentation of nuclei.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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