Caspase-6 Antibody

Images

 
Western Blot: Caspase-6 Antibody [NB100-56114] - Analysis of Caspase-6 using this antibody. Recombinant catalytically active Caspase-6 was western blotted with Active/Cleaved Caspase-6 antibody. The antibody detected ...read more
Immunohistochemistry-Paraffin: Caspase-6 Antibody [NB100-56114] - Formalin-fixed paraffin-embedded tissue sections of human gliomas stained for Active/Cleaved Caspase-6 expression using Caspase-6 antibody at 1:2000. ...read more
Immunohistochemistry: Caspase-6 Antibody [NB100-56114] - Formalin-fixed paraffin-embedded tissue section of dog cerebellum stained for Caspase-6 expression at 1:2000. Hematoxylin-eosin counterstain.

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Caspase-6 Antibody Summary

Immunogen
Recombinant catalytically active human Caspase-6 protein was used as immunogen.
Specificity
This polyclonal antisera recognizes the large and small subunits of active/cleaved caspase-6. Whereas the antisera has a strong preference for active/cleaved caspase-6, in some cell or tissue systems or techniques the antisera may also recognize the ~34 kDa proform of caspase-6 as well as intermediate caspase-6 cleavage fragments.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CASP6
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
  • Western Blot 1:1000-1:2000
Application Notes
Immunohistochemistry (frozen): Users should optimize according to model and immunodetection system used (secondary reagents).
Control
Caspase-6 Overexpression Lysate
Jurkat Staurosporine Treated / Untreated Cell Lysate
Publications
Read Publications using
NB100-56114 in the following applications:

Reactivity Notes

Cross reacts with Dog, Gerbil, Human, Mouse and Rat. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
50 ul neat whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for Caspase-6 Antibody

  • Apoptotic protease Mch-2
  • CASP6
  • CASP-6
  • caspase 6, apoptosis-related cysteine peptidase
  • caspase 6, apoptosis-related cysteine protease
  • Caspase6
  • Caspase-6
  • EC 3.4.22
  • Mch2
  • MCH2EC 3.4.22.59

Background

Caspases belong to the cysteine-aspartic acid protease (Caspase) family which plays a major role in the transduction of the apoptotic signal and execution of apoptosis in mammalian cells. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein could be processed by caspases 7, 8 and 10, and is thought to function as a downstream. Caspase-3 (CASP3/CPP32) and Caspase-6 (CASP6/Mch2) are the major active caspases in apoptotic cells and are activated in response to distinct apoptosis-inducing stimuli and in all cell, lines analyzed. Both CPP32 and Mch2 are present in apoptotic cells as multiple active species. Mch2 cleaves nuclear mitotic apparatus protein (NuMA) and mediates the shrinkage and fragmentation of nuclei.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Caspase-6 Antibody (NB100-56114)(4)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Canine.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(2)
IHC
(1)
IHC-Fr
(1)
IHC-P
(1)
WB
(2)
All Applications
Filter By Species
Human
(2)
Mouse
(2)
Rat
(1)
Canine
(1)
All Species

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Caspase-6 Antibody (NB100-56114) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on Caspase-6.

Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.

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Bioinformatics

Gene Symbol CASP6
Entrez