Caspase-6 Antibody


Western Blot: Caspase-6 Antibody [NB100-56114] - Analysis of Caspase-6 using this antibody. Recombinant catalytically active Caspase-6 was western blotted with Active/Cleaved Caspase-6 antibody. The antibody detected more
Immunohistochemistry-Paraffin: Caspase-6 Antibody [NB100-56114] - Formalin-fixed paraffin-embedded tissue sections of human gliomas stained for Active/Cleaved Caspase-6 expression using Caspase-6 antibody at 1:2000. more
Immunohistochemistry: Caspase-6 Antibody [NB100-56114] - Formalin-fixed paraffin-embedded tissue section of dog cerebellum stained for Caspase-6 expression at 1:2000. Hematoxylin-eosin counterstain.

Product Details

Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, ICC/IF, IHC, IP

Order Details

Caspase-6 Antibody Summary

Recombinant catalytically active human Caspase-6 protein was used as immunogen.
This polyclonal antisera recognizes the large and small subunits of active/cleaved caspase-6. Whereas the antisera has a strong preference for active/cleaved caspase-6, in some cell or tissue systems or techniques the antisera may also recognize the ~34 kDa proform of caspase-6 as well as intermediate caspase-6 cleavage fragments.
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  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
  • Western Blot 1:1000-1:2000
Application Notes
Immunohistochemistry (frozen): Users should optimize according to model and immunodetection system used (secondary reagents).
Caspase-6 Overexpression Lysate
Jurkat Staurosporine Treated / Untreated Cell Lysate
Read Publications using
NB100-56114 in the following applications:

Reactivity Notes

Cross reacts with Dog, Gerbil, Human, Mouse and Rat. Not yet tested in other species.

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
50 ul neat whole antisera
0.05% Sodium Azide

Alternate Names for Caspase-6 Antibody

  • Apoptotic protease Mch-2
  • CASP6
  • CASP-6
  • caspase 6, apoptosis-related cysteine peptidase
  • caspase 6, apoptosis-related cysteine protease
  • Caspase6
  • Caspase-6
  • EC 3.4.22
  • Mch2
  • MCH2EC


Caspases belong to the cysteine-aspartic acid protease (Caspase) family which plays a major role in the transduction of the apoptotic signal and execution of apoptosis in mammalian cells. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein could be processed by caspases 7, 8 and 10, and is thought to function as a downstream. Caspase-3 (CASP3/CPP32) and Caspase-6 (CASP6/Mch2) are the major active caspases in apoptotic cells and are activated in response to distinct apoptosis-inducing stimuli and in all cell, lines analyzed. Both CPP32 and Mch2 are present in apoptotic cells as multiple active species. Mch2 cleaves nuclear mitotic apparatus protein (NuMA) and mediates the shrinkage and fragmentation of nuclei.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Caspase-6 Antibody (NB100-56114)(4)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Canine.

We have publications tested in 5 applications: ICC/IF, IF/IHC, IHC-Fr, IHC-P, WB.

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Showing Publications 1 - 4 of 4.
Publications using NB100-56114 Applications Species
Berta T, Park CK, Xu ZZ et al. Extracellular caspase-6 drives murine inflammatory pain via microglial TNF-alpha secretion. J. Clin. Invest. 2014-03-03 [PMID: 24531553] (WB, Mouse)

WB (mouse spinal cord tissue, cerebral fluid and recombinant Caspase-6), Figs 2C-F, S1. The Caspase-6 pAb was used at 1:2000. The specificity of the antibody was validated with recombinant caspase-6 by WB (Fig 2E). Pro-caspase-6 was detected at ~37kDa, an
WB Mouse
Saggar JK, Tannock IF. Activity of the hypoxia-activated pro-drug TH-302 in hypoxic and perivascular regions of solid tumors and its potential to enhance therapeutic effects of chemotherapy. Int J Cancer. 2013-11-08 [PMID: 24338277] (IHC-Fr, ICC/IF, Human) IHC-Fr, ICC/IF Human
Saggar JK, Fung AS, Patel KJ, Tannock IFet al. Use of molecular biomarkers to quantify the spatial distribution of effects of anticancer drugs in solid tumors. Mol Cancer Ther 2013-04-01 [PMID: 23348047] (ICC/IF, IF/IHC, Human) ICC/IF, IF/IHC Human
Krajewska M, Rosenthal RE, Mikolajczyk J et al. Early processing of Bid and caspase-6, -8, -10, -14 in the canine brain during cardiac arrest and resuscitation. Exp Neurol. 2004-10-01 [PMID: 15380478] (IHC-P, WB, Rat, Canine, Mouse)

Antibodies cited: 1. Caspase-9 (Active/Cleaved), IMG-5705: WB: Fig 1A (recombinant human caspase-9), Fig 4A (recombinant human caspase-9, rat brain), Fig 4B-F (isolated mitochondria from rat liver or heart). Fig 5 (PC12 cells) IHC (P): Fig 2d-k (rat kidne
IHC-P, WB Rat, Canine, Mouse

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies


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Gene Symbol CASP6