MSH3 Antibody (CL14059) - BSA Free

Staining of human breast cancer shows moderate to strong nuclear positivity in tumor cells.

Western blot analysis in human cell line U-251 MG.

Staining of human cervix, uterine shows moderate nuclear positivity in squamous epithelial cells.

Staining of human cervical cancer shows strong nuclear positivity in tumor cells.

Staining of human testis shows no positivity in cells in seminiferous ducts.

Staining of human ovarian cancer (papillary ovarian carcinoma) shows moderate nuclear positivity in tumor cells.

Staining in RH-30 cell line with Anti-MSH3 monoclonal antibody, showing specific staining of nucleoplasm and nucleus in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Staining of human kidney shows moderate nuclear positivity in cells in tubules and cells in glomeruli.

Staining of human colon shows moderate nuclear positivity in glandular and lymphoid cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: CL14059
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

MSH3 Antibody (CL14059) - BSA Free Summary

• Description: Novus Biologicals Mouse MSH3 Antibody (CL14059) - BSA Free (NBP3-24587) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a synthetic peptide derived from P20585, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MSH3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1ug/ml
• Application Notes: ICC/IF, PFA/Triton X-100, IHC-P, Retrieval method: HIER pH6

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for MSH3 Antibody (CL14059) - BSA Free

• Divergent upstream protein
• DNA mismatch repair protein Msh3
• DUC1
• DUG
• DUPMGC163306
• hMSH3
• Mismatch repair protein 1
• MRP1MGC163308
• mutS (E. coli) homolog 3
• mutS homolog 3 (E. coli)

Background

The function of MSH3 is unknown. It is probably a DNA-repair protein. Defects in MSH3 are a cause of susceptibility to endometrial cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MSH3