Caspase 5 Antibody [Alexa Fluor™ Plus 647]

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor Plus 647

Caspase 5 Antibody [Alexa Fluor™ Plus 647] Summary

• Immunogen: Antibody was raised against a 14 amino acid synthetic peptide from the center of human Caspase-5. The immunogen is located within amino acids 170 - 220 of Caspase-5. Amino Acid Squence: YPIKKREDRRRLAL
• Specificity: Depending on cell lines or tissues used, other cleavage products may be observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CASP5
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Peptide affinity purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Caspase 5 Antibody [Alexa Fluor™ Plus 647]

• CASP5
• CASP-5
• caspase 5, apoptosis-related cysteine peptidase
• caspase 5, apoptosis-related cysteine protease
• Caspase5
• Caspase-5
• EC 3.4.22
• EC 3.4.22.58
• ICE(rel)III
• ICE(rel)-III
• ICEREL-III
• ICH3
• ICH-3
• MGC141966
• Protease ICH-3
• Protease TY
• TY protease

Background

Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-5 (ICH-3, ICEREL-III, TY protease) is a member of the caspase-1 subfamily and it is an upstream mediator of apoptosis. Caspase-5 is highly expressed in lung, liver, and skeletal muscle, and similar to Caspase-4, its overexpression can induce cellular apoptosis (1). A tetramer, Caspase-5 separates into two subunits of a 20kda (p20) and 10kda (p10) after activation. In vitro, Caspase-5 can be cleaved by GranB, a major player in Cytotoxic T lymphocytes (CTL) induced apoptosis (2). In vivo, the Caspase-5 expression is elevated by LPS and IFNg. Caspase-5 induced apoptosis can be inhibited by CrmA and Bcl-2. Caspase-5 has also been implicated in cellular inflammatory response and in the microsatelite mutator pathway for cancer (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CASP5