Caspase-11 Antibody (17D9)

Images

 
Western Blot: Caspase-11 Antibody (17D9) [NB120-10454] - Analysis of Caspase-11 in THP-1 cell lysates that were untreated (left lane) or treated (right lane) with 100 ng/ml LPS. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Caspase 11 Antibody (17D9) [NB120-10454] - Caspase 11 antibody was tested in NIH-3T3 cells at a 1:100 dilution using a Dylight 488 conjugated secondary antibody (Green). Actin ...read more
Immunohistochemistry-Paraffin: Caspase 11 Antibody (17D9) [NB120-10454] - IHC-P analysis of a formalin fixed paraffin embedded tissue section of mouse lung using Caspase 11 antibody (clone 17D9) at 1:100 diluton. The ...read more
Western Blot: Caspase 11 Antibody (17D9) [NB120-10454] - Mouse B cell. Image from verified customer review.
Western Blot: Caspase 11 Antibody (17D9) [NB120-10454] - Mouse liver extract was separated on a 4-15% gel and transfered to PVDF membrane. The membrane was probed with anti-caspase 11 antibody and detected with an ...read more
Western Blot: Caspase-11 Antibody (17D9) [NB120-10454] - Caspase 11 Antibody (17D9) [NB120-10454] - analysis of Caspase 11 in LPS stimulated bone marrow derived macrophages using anti-Caspase 11 antibody. Image from ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, MiAr
Clone
17D9
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

Caspase-11 Antibody (17D9) Summary

Immunogen
A recombinant p30 subunit of mouse Caspase 11. [UniProt# P70343]
Localization
Cytoplasmic (Endoplasmic reticulum membrane and Mitochondrion)
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
CASP11
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 5 ug/ml
  • ELISA
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation
  • Microarray
Application Notes
Use in immunprecipitation reported in scientific literature (PMID 10791975) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
35-43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 4 Reviews rated 4.3
using
NB120-10454 in the following applications:

Publications
Read Publications using
NB120-10454 in the following applications:

  • 1 publication
  • IB
    1 publication
  • IHC
    2 publications
  • IP
    2 publications
  • WB
    17 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Background

Caspases are cysteine proteases that play an essential role in apoptosis; their regulation is post-translational to enable rapid activation. Murine Caspase 11, also known as Caspase 4 or ICH3, has a heterotetrameric structure consisting of two anti-parallel heterodimers, each one formed of a large (p20) and a small (p10) subunit. Each heterodimer has a weight of 43kDa. Upon activation, murine caspase 11 is cleaved by an autocatalytic mechanism to give rise to the individual subunits. Expression levels of murine Caspase 11 are highest in lung and spleen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, MiAr

Publications for Caspase-11 Antibody (NB120-10454)(22)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ELISA, IB, IHC, IP, WB.


Filter By Application
ELISA
(1)
IB
(1)
IHC
(2)
IP
(2)
WB
(17)
All Applications
Filter By Species
Human
(1)
Mouse
(17)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using NB120-10454 Applications Species
Cheng KT, Xiong S, Ye Z et al. Caspase-11-mediated endothelial pyroptosis underlies endotoxemia-induced lung injury. J. Clin. Invest. 2017 Nov 01 [PMID: 28990935] (WB, Mouse) WB Mouse
Ming Man S, Karki R, Briard B et al. Differential roles of caspase-1 and caspase-11 in infection and inflammation. Sci Rep. Mar 27 2017 [PMID: 28345580] (IB, Mouse) IB Mouse
Jin HY, Oda H, Chen P et al. Differential Sensitivity of Target Genes to Translational Repression by miR-17-92. PLoS Genet. [PMID: 28241004] (WB, Mouse) WB Mouse
Klaska IP, Muckersie E, Martin-Granados C et al. LPS-primed Heterotolerant Dendritic Cells suppress Experimental Autoimmune Uveoretintitis by multiple mechanisms. Immunology. Nov 15 2016 [PMID: 27859049] (WB, Mouse) WB Mouse
Kim MJ, Bae SH, Ryu JC et al. SESN2/sestrin2 suppresses sepsis by inducing mitophagy and inhibiting NLRP3 activation in macrophages. Autophagy Jun 23 2016 [PMID: 27337507] (WB, IP) WB, IP
Chung H, Vilaysane A, Lau A et al. NLRP3 regulates a non-canonical platform for caspase-8 activation during epithelial cell apoptosis. Cell Death Differ. 2016 Feb 19 [PMID: 26891693] (WB, Mouse) WB Mouse
Kayagaki N, Stowe IB, Lee BL et al. Caspase-11 cleaves gasdermin D for non-canonical inflammasome signaling. Nature 2015 Sep 16 [PMID: 26375259] (WB, Mouse) WB Mouse
Shi J, Zhao Y, Wang K et al. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death. Nature 2015 Sep 16 [PMID: 26375003] (WB, Mouse) WB Mouse
Aachoui Y, Kajiwara Y, Leaf IA et al. Canonical Inflammasomes Drive IFN-gamma to Prime Caspase-11 in Defense against a Cytosol-Invasive Bacterium. Cell Host Microbe 2015 Sep 09 [PMID: 26320999] (WB, Mouse) WB Mouse
Bonora M, Wieckowsk MR, Chinopoulos C et al. Molecular mechanisms of cell death: central implication of ATP synthase in mitochondrial permeability transition. Oncogene 2015 Mar 19 [PMID: 25790189]
Show All 22 Publications.

Reviews for Caspase-11 Antibody (NB120-10454) (4) 4.34

Average Rating: 4.3
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, Mouse.
We have 4 reviews tested in 1 application: WB.

Reviews using NB120-10454:
Filter by Applications
WB
(4)
All Applications
Filter by Species
Human
(1)
Mouse
(3)
All Species
Images Ratings Applications Species Date Details
Western Blot Caspase-11 NB120-10454
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reviewed by:
Anonymous
WB Human 08/06/2016
View

Summary

ApplicationWestern Blot
SpeciesHuman
LotPO number: P00129026
FileView PDF
Western Blot Caspase-11 NB120-10454
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reviewed by:
Anonymous
WB Mouse 02/02/2016
View

Summary

ApplicationWestern Blot
Sample Testedmouse macrophage
SpeciesMouse
LotA-6

Blocking

Blocking Details5% skim milk in PBS-T buffer

Primary Anitbody

Dilution Ratio4 degree overnight

Secondary Antibody

Secondary DescriptionGoat anti-rat IgG, HRP (Abcam)
Secondary Concentration1:4,000

Details

Detection NotesECL

Comments

Commentsthis is a very good antibody for pro-Caspase 11 detection, its specificity against the activated form of caspase 11 remains unknown, at least we didn't see a strong potency for the detection of activated form through western blot.
Western Blot Caspase-11 NB120-10454
Enlarge
reviewed by:
Anonymous
WB Mouse 03/19/2015
View

Summary

ApplicationWestern Blot
Sample TestedBone Marrrow Derived Macrophages
SpeciesMouse
Lot102M4832

Blocking

Blocking Details5% Milk

Primary Anitbody

Dilution Ratio1:1000; overnight; 4C, 2.5%Milk/TBST

Secondary Antibody

Secondary DescriptionGoat anti-Rat
Secondary Concentration1:10000

Details

Detection NotesHRP, 3min, LPS untreated negative control
Western Blot Caspase-11 NB120-10454
Enlarge
reviewed by:
Hyun Yong Jin
WB Mouse 07/01/2014
View

Summary

ApplicationWestern Blot
SpeciesMouse

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Caspase-11 Antibody (NB120-10454). (Showing 1 - 2 of 2 FAQs).

  1. If I dilute the 100 uL of antibody (the whole delivered content) in 10 mL of buffer to incubate my blottet membrane in (and I need this volume of about 10 mL for the membrane to float in it on the shaker). I will be able to re-use this one dilution (which is 10 ug/mL) for 100 western blots? So that means for 100 samples? Or even for 100 membranes? Usually we use a antibody-dilution of 1:1000 (around 1ug/mL), this would be 1:100 (10ug/mL).
    • I am hoping I can assist you in determining usage of this antibody in your assay. The antibody comes at a starting concentration of 1 mg/mL. Since you get 100uL in the vial you will have 100 ug of antibody. The lab recommends using a dilution of 1:100-1:200 for WB. Therefore if you wished to use 10 mL volume on your incubation you would need to add 100 uL of antibody into 9.9mL of buffer to give you a final concentration of 10 ug/uL. Since you are using such a large final volume you will only get 1-2 blots per Western ran since we do not recommend reusing your antibody. If you can run the blots with much lower final volume such as 1 mL instead you can get away with a lot more Westerns.
  2. If it has been determined, can you let me know as specifically as possible the epitope that the Caspase-11 antibody recognizes, eg. peptide sequence or approximate region of the p30 subunit?
    • This antibody has not been epitope mapped and was generated using full-length p30. The only information we have is that the antibody may be used for immunoblotting [to detect the full length caspase 11 (apparent size of 35-43 kDa), and the p10 and p30fragments], so it must recognize a region similar in both subunits.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB120-10454AF405
Alexa Fluor 488 Labeled NB120-10454AF488
Alexa Fluor 647 Labeled NB120-10454AF647
Alexa Fluor 700 Labeled NB120-10454AF700
Biotin Labeled NB120-10454B
DyLight 350 Labeled NB120-10454UV
DyLight 405 Labeled NB120-10454V
DyLight 488 Labeled NB120-10454G
DyLight 550 Labeled NB120-10454R
DyLight 650 Labeled NB120-10454C
DyLight 680 Labeled NB120-10454FR
DyLight 755 Labeled NB120-10454IR
HRP Labeled NB120-10454H

Additional Caspase-11 Products

Caspase-11 NB120-10454

Bioinformatics Tool for Caspase-11 Antibody (NB120-10454)

Discover related pathways, diseases and genes to Caspase-11 Antibody (NB120-10454). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Caspase-11.

Caspase 11 - A proinflammatory caspase that induces the innate immune response
While known for their role in programmed cell death, caspases are also essential for mediating inflammatory responses and innate immunity. Binding of microbial molecules by pattern recognition receptors triggers the formation of the multiprotein in...  Read full blog post.

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Recent Reviews

4.3
5
1
4
3
3
0
2
0
1
0

Anonymous
08/06/2016
Application: WB
Species: Human

Anonymous
02/02/2016
Application: WB
Species: Mouse

Anonymous
03/19/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol CASP11
Uniprot