Caspase-11 Antibody (17D9) - BSA Free

Images

 
Western Blot: Caspase-11 Antibody (17D9) [NB120-10454] - Mouse liver extract was separated on a 4-15% gel and transfered to PVDF membrane. The membrane was probed with anti-caspase 11 antibody and detected with an ...read more
Immunohistochemistry-Paraffin: Caspase-11 Antibody (17D9) [NB120-10454] - IHC-P analysis of a formalin fixed paraffin embedded tissue section of mouse lung using Caspase 11 antibody (clone 17D9) at 1:100 diluton. The ...read more
Biological Strategies: Western Blot: Caspase-11 Antibody (17D9) [NB120-10454] - Analysis of Caspase-11 in THP-1 cell lysates that were untreated (left lane) or treated (right lane) with 100 ng/ml LPS. Image from ...read more
Immunocytochemistry/ Immunofluorescence: Caspase-11 Antibody (17D9) [NB120-10454] - Caspase 11 antibody was tested in NIH-3T3 cells at a 1:100 dilution using a Dylight 488 conjugated secondary antibody (Green). Actin ...read more
Biological Strategies: Western Blot: Caspase-11 Antibody (17D9) [NB120-10454] - Caspase 11 Antibody (17D9) [NB120-10454] - analysis of Caspase 11 in LPS stimulated bone marrow derived macrophages using ...read more
Knockout Validated: Caspase-11 Antibody (17D9) [NB120-10454] - Casp11fl/fl and Casp11EC-/- mice (n = 3) were challenged with LPS (40 mg/kg i.p.) for 6 hours. Endothelial Cells were isolated from lungs, and western blot ...read more
Knockout Validated: Caspase-11 Antibody (17D9) [NB120-10454] - CNF1-triggered immunity requires inflammatory caspases.(A and B) IL-1beta production and maturation/secretion after treatment with CNF1, LPS or CNF1 (1 ...read more
Knockout Validated: Caspase-11 Antibody (17D9) [NB120-10454] - CNF1-triggered IL-1beta maturation requires activated Rac, ASC and caspase-1. Western blot analysis of the production and maturation/secretion of IL-1beta ...read more
Knockout Validated: Caspase-11 Antibody (17D9) [NB120-10454] - Caspase-11 is expressed in Casp1Null bone marrow-derived macrophages. Immunoblot analysis of caspase-1, caspase-11 and GAPDH (loading control) in unprimed ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ELISA, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, MiAr, KD, KO
Clone
17D9
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

Caspase-11 Antibody (17D9) - BSA Free Summary

Immunogen
A recombinant p30 subunit of mouse Caspase 11. [UniProt# P70343]
Localization
Cytoplasmic (Endoplasmic reticulum membrane and Mitochondrion)
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
Casp4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation
  • Knockdown Validated
  • Knockout Validated
  • Microarray
  • Western Blot 5 ug/ml
Application Notes
Use in immunprecipitation (PMID 10791975) and immunoblotting (PMID: 28345580) reported in scientific literature. Use in FLOW reported in scientific literature (PMID: 27708283).
Theoretical MW
35-43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Jurkat Staurosporine Treated / Untreated Cell Lysate
Reviewed Applications
Read 6 Reviews rated 4
using
NB120-10454 in the following applications:

Publications
Read Publications using
NB120-10454 in the following applications:

  • 1 publication
  • 2 publications
  • IB
    4 publications
  • IHC
    4 publications
  • 1 publication
  • 1 publication
  • IP
    3 publications
  • PLA
    1 publication
  • WB
    38 publications

Reactivity Notes

Use in Rat reported in scientific literature (PMID:32318575). Use in Bovine reported in scientific literature (PMID:31156617).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Caspase-11 Antibody (17D9) - BSA Free

  • ich-3
  • CASP11
  • CASP-11
  • CASP4
  • CASP-4
  • caspase 4, apoptosis-related cysteine peptidase
  • Caspl

Background

Caspases are cysteine proteases that play an essential role in apoptosis, and their regulation is post-translational to enable rapid activation. Murine Caspase 11, also known as Caspase 4 or ICH3, has a heterotetrameric structure consisting of two anti-parallel heterodimers, each one formed of a large (p20) and a small (p10) subunit. Each heterodimer has a weight of 43kDa. Upon activation, murine caspase 11 is cleaved by an autocatalytic mechanism to give rise to the individual subunits. Expression levels of murine Caspase 11 are highest in the lung and spleen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Caspase-11 Antibody (NB120-10454)(57)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, E. coli.

We have publications tested in 9 applications: ELISA, Flow, IB, IHC, IHC-Fr, IHC-P, IP, PLA, WB.


Filter By Application
ELISA
(1)
Flow
(2)
IB
(4)
IHC
(4)
IHC-Fr
(1)
IHC-P
(1)
IP
(3)
PLA
(1)
WB
(38)
All Applications
Filter By Species
Human
(6)
Mouse
(41)
Rat
(1)
Bovine
(1)
E. coli
(1)
All Species
Showing Publications 1 - 10 of 57. Show All 57 Publications.
Publications using NB120-10454 Applications Species
Sundaram B, Karki R, Kanneganti TD NLRC4 Deficiency Leads to Enhanced Phosphorylation of MLKL and Necroptosis ImmunoHorizons Mar 17 2022 [PMID: 35301258] (WB, Mouse) WB Mouse
Ma C, Yang D, Wang B et al. Gasdermin D in macrophages restrains colitis by controlling cGAS-mediated inflammation Sci. Adv. May 1 2020 (WB, Mouse) WB Mouse
Wang Z, Yang X, Wang X et al. Glycyrrhizin attenuates caspase-11-dependent immune responses and coagulopathy by targeting high mobility group box 1 International immunopharmacology Mar 24 2022 [PMID: 35339905] (WB, IHC, PLA, Mouse, Human) WB, IHC, PLA Mouse, Human
Huang D, Chen P, Huang G et al. Salt-inducible kinases inhibitor HG-9-91-01 targets RIPK3 kinase activity to alleviate necroptosis-mediated inflammatory injury Cell death & disease Feb 25 2022 [PMID: 35217652] (WB, Mouse) WB Mouse
Zheng M, Karki R, Kancharana B Et al. Caspase-6 promotes activation of the caspase-11-NLRP3 inflammasome during gram-negative bacterial infections Journal of Biological Chemistry Nov 1 2021 [PMID: 34740613] (WB) WB
Karki R, Sundaram B, Sharma BR Et al. ADAR1 restricts ZBP1-mediated immune response and PANoptosis to promote tumorigenesis Cell reports Oct 19 2021 [PMID: 34686350] (WB, Mouse) WB Mouse
Xiong S, Zhang L, Richner JM et al. Interleukin-1RA Mitigates SARS-CoV-2-Induced Inflammatory Lung Vascular Leakage and Mortality in Humanized K18-hACE-2 Mice Arteriosclerosis, thrombosis, and vascular biology Sep 9 2021 [PMID: 34496633]
Fan R, Sui J, Dong X et al. Wedelolactone alleviates acute pancreatitis and associated lung injury via GPX4 mediated suppression of pyroptosis and ferroptosis Free radical biology & medicine Jul 8 2021 [PMID: 34246777] (WB) WB
Wang X, Li Z, Bai Y et al. A small molecule binding HMGB1 inhibits caspase-11-mediated lethality in sepsis Cell death & disease Apr 14 2021 [PMID: 33854044]
Chen Q, Zheng J, Wang D, et al. Nitrosonisoldipine is a selective inhibitor of inflammatory caspases and protects against pyroptosis and related septic shock European journal of immunology Jan 17 2021 [PMID: 33454984] (WB, Mouse) WB Mouse
Show All 57 Publications.

Reviews for Caspase-11 Antibody (NB120-10454) (6) 46

Average Rating: 4
(Based on 6 reviews)
We have 6 reviews tested in 2 species: Human, Mouse.

Reviews using NB120-10454:
Filter by Applications
Western Blot
(6)
All Applications
Filter by Species
Human
(1)
Mouse
(5)
All Species
Images Ratings Applications Species Date Details
  3
reviewed by:
Kyohei Nishino
Western Blot Mouse 03/31/2018
View

Summary

ApplicationWestern Blot
Sample TestedMouse Colon
SpeciesMouse
LotA-10
Western Blot Caspase-11 NB120-10454
Enlarge
4
reviewed by:
Liyong Zhang
Western Blot Mouse 12/18/2017
View

Summary

ApplicationWestern Blot
Sample Testedmouse primary hepatocytes
SpeciesMouse
LotA-8

Comments

Comments100 ng/ml LPS treated for 1 hour
Name: Anti-caspase 11 antibody (NB120-10454)
Catalog #: Anti-caspase 11 antibody (NB120-10454)
Lot Number: Anti-caspase 11 antibody (NB120-10454, Lot # A-6)
PO/Order Number: Click here to enter text..
WB Image Description (Please provide labels for all lanes): mouse primary hepatocytes treated without (lane 1), or with 100 ng/ml LPS (lane 2)
Sample Information:
     Cell Line or Tissue: mouse primary hepatocytes
     Species: mouse
     Treatment: 100 ng/ml LPS
Lysate Preparation:
     Date of lysate preparation: December 4, 2017
     Lysis buffer used: 1X lysis buffer from Cell Signaling by adding PMSF
Reducing agent: beta-mercaptoethanol, DTT
If boiled (temperature/time): Yes
Controls:
     Positive Control: No
     Negative Control: No
     Loading Control (please attach additional images if applicable): No
Protein Amount Loaded per lane: 20 ug
Antibody Storage Conditions: -20℃
Electrophoresis:
Gel Percentage: 12%
Electrophoresis Conditions: Tris-Glycine-SDS at room temperature
     Voltage: 120V
     Time: 2 hours
Membrane Transfer:
     Method (Submersion/Semi-dry): wet transfer
     Membrane Type (PVDF/Nitrocellulose): Nitrocellulose
     Time: 2 hours
     Voltage: 100V
Blocking:
     Blocking Solution: 5% milk in 1X TBST
     Time: 1 hour at room temperature
Primary Antibody:
     Dilution: 1/200
     Diluent Buffer: 2.5% BSA
     Incubation Time: overnight
     Incubation Temperature: 4℃
Washing Conditions:
     Wash Solution: 1X TBST
     Time and Repetitions: 5 min each for 3 times
Secondary Antibody
Manufacturer and Catalog #: Thermo Scientific, cat # 34095, Lot # SG251180
Secondary description: goat anti-rat secondary antibody
     Dilution: 1/5000
     Diluent Buffer: 3% milk
     Incubation Time: 1 hour
     Incubation Temperature: room temperature
Detection Method:
Detection: ECL (GE, cat # RPN2209, lot #n9838243)
Procedure: Add equal volume of A and B, mix and apply on the membranes for 3-5 min before exposure
Development Time: 25 seconds
Molecular weight of band(s): ~ 38, 43 kDa
Experimental Concerns and Observations: Specific bands around 38, 43 kDa were observed
Western Blot Caspase-11 NB120-10454
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5
reviewed by:
Liyong Zhang
Western Blot Human 08/06/2016
View

Summary

ApplicationWestern Blot
SpeciesHuman
LotPO number: P00129026
Western Blot Caspase-11 NB120-10454
Enlarge
4
reviewed by:
Eric Wang
Western Blot Mouse 02/02/2016
View

Summary

ApplicationWestern Blot
Sample Testedmouse macrophage
SpeciesMouse
LotA-6
Western Blot Caspase-11 NB120-10454
Enlarge
4
reviewed by:
Kim Nguyen
Western Blot Mouse 03/19/2015
View

Summary

ApplicationWestern Blot
Sample TestedBone Marrrow Derived Macrophages
SpeciesMouse
Lot102M4832
Western Blot Caspase-11 NB120-10454
Enlarge
4
reviewed by:
Verified Customer
Western Blot Mouse 07/01/2014
View

Summary

ApplicationWestern Blot
SpeciesMouse

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Caspase-11 Antibody (NB120-10454). (Showing 1 - 2 of 2 FAQs).

  1. If I dilute the 100 uL of antibody (the whole delivered content) in 10 mL of buffer to incubate my blottet membrane in (and I need this volume of about 10 mL for the membrane to float in it on the shaker). I will be able to re-use this one dilution (which is 10 ug/mL) for 100 western blots? So that means for 100 samples? Or even for 100 membranes? Usually we use a antibody-dilution of 1:1000 (around 1ug/mL), this would be 1:100 (10ug/mL).
    • I am hoping I can assist you in determining usage of this antibody in your assay. The antibody comes at a starting concentration of 1 mg/mL. Since you get 100uL in the vial you will have 100 ug of antibody. The lab recommends using a dilution of 1:100-1:200 for WB. Therefore if you wished to use 10 mL volume on your incubation you would need to add 100 uL of antibody into 9.9mL of buffer to give you a final concentration of 10 ug/uL. Since you are using such a large final volume you will only get 1-2 blots per Western ran since we do not recommend reusing your antibody. If you can run the blots with much lower final volume such as 1 mL instead you can get away with a lot more Westerns.
  2. If it has been determined, can you let me know as specifically as possible the epitope that the Caspase-11 antibody recognizes, eg. peptide sequence or approximate region of the p30 subunit?
    • This antibody has not been epitope mapped and was generated using full-length p30. The only information we have is that the antibody may be used for immunoblotting [to detect the full length caspase 11 (apparent size of 35-43 kDa), and the p10 and p30fragments], so it must recognize a region similar in both subunits.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Caspase-11 Antibody (NB120-10454)

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By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.

Caspase 11 - A proinflammatory caspase that induces the innate immune response
While known for their role in programmed cell death, caspases are also essential for mediating inflammatory responses and innate immunity. Binding of microbial molecules by pattern recognition receptors triggers the formation of the multiprotein in...  Read full blog post.

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Recent Reviews

4
5
1
4
4
3
1
2
0
1
0

 
Kyohei Nishino
03/31/2018
Application: Western Blot
Species: Mouse

Liyong Zhang
12/18/2017
Application: Western Blot
Species: Mouse

Liyong Zhang
08/06/2016
Application: Western Blot
Species: Human

Bioinformatics

Gene Symbol Casp4
Uniprot