Caspase-11 Antibody (17D9)

Images

 
Western Blot: Caspase-11 Antibody (17D9) [NB120-10454] - Mouse liver extract was separated on a 4-15% gel and transfered to PVDF membrane. The membrane was probed with anti-caspase 11 antibody and detected with an ...read more
Immunohistochemistry-Paraffin: Caspase-11 Antibody (17D9) [NB120-10454] - IHC-P analysis of a formalin fixed paraffin embedded tissue section of mouse lung using Caspase 11 antibody (clone 17D9) at 1:100 diluton. The ...read more
Biological Strategies: Western Blot: Caspase-11 Antibody (17D9) [NB120-10454] - Analysis of Caspase-11 in THP-1 cell lysates that were untreated (left lane) or treated (right lane) with 100 ng/ml LPS. Image from ...read more
Immunocytochemistry/ Immunofluorescence: Caspase-11 Antibody (17D9) [NB120-10454] - Caspase 11 antibody was tested in NIH-3T3 cells at a 1:100 dilution using a Dylight 488 conjugated secondary antibody (Green). Actin ...read more
Biological Strategies: Western Blot: Caspase-11 Antibody (17D9) [NB120-10454] - Caspase 11 Antibody (17D9) [NB120-10454] - analysis of Caspase 11 in LPS stimulated bone marrow derived macrophages using ...read more
Knockout Validated: Caspase-11 Antibody (17D9) [NB120-10454] - Casp11fl/fl and Casp11EC-/- mice (n = 3) were challenged with LPS (40 mg/kg i.p.) for 6 hours. Endothelial Cells were isolated from lungs, and western blot ...read more
Knockout Validated: Caspase-11 Antibody (17D9) [NB120-10454] - CNF1-triggered immunity requires inflammatory caspases.(A and B) IL-1beta production and maturation/secretion after treatment with CNF1, LPS or CNF1 (1 ...read more
Knockout Validated: Caspase-11 Antibody (17D9) [NB120-10454] - CNF1-triggered IL-1beta maturation requires activated Rac, ASC and caspase-1. Western blot analysis of the production and maturation/secretion of IL-1beta ...read more
Knockout Validated: Caspase-11 Antibody (17D9) [NB120-10454] - Caspase-11 is expressed in Casp1Null bone marrow-derived macrophages. Immunoblot analysis of caspase-1, caspase-11 and GAPDH (loading control) in unprimed ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, MiAr, KD, KO
Clone
17D9
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

Caspase-11 Antibody (17D9) Summary

Immunogen
A recombinant p30 subunit of mouse Caspase 11. [UniProt# P70343]
Localization
Cytoplasmic (Endoplasmic reticulum membrane and Mitochondrion)
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
Casp4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 5 ug/ml
  • ELISA
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation
  • Microarray
  • Knockdown Validated
  • Knockout Validated
Application Notes
Use in immunprecipitation (PMID 10791975) and immunoblotting (PMID: 28345580) reported in scientific literature. Use in FLOW reported in scientific literature (PMID: 27708283).
Theoretical MW
35-43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 6 Reviews rated 4
using
NB120-10454 in the following applications:

Publications
Read Publications using
NB120-10454 in the following applications:

  • 1 publication
  • 2 publications
  • IB
    4 publications
  • IHC
    3 publications
  • 1 publication
  • 1 publication
  • IP
    3 publications
  • WB
    31 publications

Reactivity Notes

Use in Rat reported in scientific literature (PMID:32318575).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Caspase-11 Antibody (17D9)

  • ich-3
  • CASP11
  • CASP-11
  • CASP4
  • CASP-4
  • caspase 4, apoptosis-related cysteine peptidase
  • Caspl

Background

Caspases are cysteine proteases that play an essential role in apoptosis, and their regulation is post-translational to enable rapid activation. Murine Caspase 11, also known as Caspase 4 or ICH3, has a heterotetrameric structure consisting of two anti-parallel heterodimers, each one formed of a large (p20) and a small (p10) subunit. Each heterodimer has a weight of 43kDa. Upon activation, murine caspase 11 is cleaved by an autocatalytic mechanism to give rise to the individual subunits. Expression levels of murine Caspase 11 are highest in the lung and spleen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Caspase-11 Antibody (NB120-10454)(48)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, E. coli.

We have publications tested in 8 applications: ELISA, Flow, IB, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
ELISA
(1)
Flow
(2)
IB
(4)
IHC
(3)
IHC-Fr
(1)
IHC-P
(1)
IP
(3)
WB
(31)
All Applications
Filter By Species
Human
(5)
Mouse
(36)
Rat
(1)
Bovine
(1)
E. coli
(1)
All Species
Showing Publications 1 - 10 of 48. Show All 48 Publications.
Publications using NB120-10454 Applications Species
Ma C, Yang D et al. Gasdermin D in macrophages restrains colitis by controlling cGAS-mediated inflammation. Sci Adv Jan 5 2020 [PMID: 32671214] (WB, Mouse) WB Mouse
Eren E, Planes R, Buyck JM et al. Type-3 Secretion Systeminduced pyroptosis protects Pseudomonas against cell-autonomous immunity bioRxiv (WB, Mouse) WB Mouse
Selkrig J, Li N, Hausmann A et al. Spatiotemporal proteomics uncovers cathepsin-dependent macrophage cell death during Salmonella infection Nat Microbiol Jun 8 2020 [PMID: 32514074] (WB, Mouse) WB Mouse
Saxena P Role of Inflammatory Cytokine Signaling in Control of Bacterial Infection Thesis Jan 1 2020 (WB, Mouse) WB Mouse
Karki R, Lee E, Sharma Br et Al. IRF8 Regulates Gram-Negative Bacteria-Mediated NLRP3 Inflammasome Activation and Cell Death J. Immunol. Mar 23 2020 [PMID: 32205422] (IB, Mouse) IB Mouse
Finethy R, Dockterman J, Kutsch M, Orench-Rivera N Dynamin-related Irgm proteins modulate LPS-induced caspase-4 activation and septic shock bioRxiv Jan 1 2020 (IB, E. coli, Mouse) IB E. coli, Mouse
Ma C, Yang D, Wang B et al. Gasdermin D in macrophages restrains colitis by controlling cGAS-mediated inflammation Sci. Adv. May 1 2020 (WB, Mouse) WB Mouse
Karki R, Sharma BR, Tuladhar S et al. Synergism of TNF- alpha and IFN-gamma Triggers Inflammatory Cell Death, Tissue Damage, and Mortality in SARS-CoV-2 Infection and Cytokine Shock Syndromes Cell Nov 19 2020 [PMID: 33278357] (WB, Mouse) WB Mouse
Wang J, Li X, Liu Y et al. CircHIPK3 Promotes Pyroptosis in Acinar Cells Through Regulation of the miR-193a-5p/GSDMD Axis Front Med (Lausanne) Apr 7 2020 [PMID: 32318575] (Flow, Rat) Flow Rat
Huang LS, Hong Z, Wu W et al. mtDNA Activates cGAS Signaling and Suppresses the YAP-Mediated Endothelial Cell Proliferation Program to Promote Inflammatory Injury Immunity Mar 17 2020 [PMID: 32164878]
Show All 48 Publications.

Reviews for Caspase-11 Antibody (NB120-10454) (6) 46

Average Rating: 4
(Based on 6 reviews)
We have 6 reviews tested in 2 species: Human, Mouse.

Reviews using NB120-10454:
Filter by Applications
WB
(6)
All Applications
Filter by Species
Human
(1)
Mouse
(5)
All Species
Images Ratings Applications Species Date Details
  3
reviewed by:
Anonymous
WB Mouse 03/31/2018
View

Summary

ApplicationWestern Blot
Sample TestedMouse Colon
SpeciesMouse
LotA-10
Western Blot Caspase-11 NB120-10454
Enlarge
4
reviewed by:
Anonymous
WB Mouse 12/18/2017
View

Summary

ApplicationWestern Blot
Sample Testedmouse primary hepatocytes
SpeciesMouse
LotA-8

Comments

Comments100 ng/ml LPS treated for 1 hour
Name: Anti-caspase 11 antibody (NB120-10454)
Catalog #: Anti-caspase 11 antibody (NB120-10454)
Lot Number: Anti-caspase 11 antibody (NB120-10454, Lot # A-6)
PO/Order Number: Click here to enter text..
WB Image Description (Please provide labels for all lanes): mouse primary hepatocytes treated without (lane 1), or with 100 ng/ml LPS (lane 2)
Sample Information:
     Cell Line or Tissue: mouse primary hepatocytes
     Species: mouse
     Treatment: 100 ng/ml LPS
Lysate Preparation:
     Date of lysate preparation: December 4, 2017
     Lysis buffer used: 1X lysis buffer from Cell Signaling by adding PMSF
Reducing agent: beta-mercaptoethanol, DTT
If boiled (temperature/time): Yes
Controls:
     Positive Control: No
     Negative Control: No
     Loading Control (please attach additional images if applicable): No
Protein Amount Loaded per lane: 20 ug
Antibody Storage Conditions: -20℃
Electrophoresis:
Gel Percentage: 12%
Electrophoresis Conditions: Tris-Glycine-SDS at room temperature
     Voltage: 120V
     Time: 2 hours
Membrane Transfer:
     Method (Submersion/Semi-dry): wet transfer
     Membrane Type (PVDF/Nitrocellulose): Nitrocellulose
     Time: 2 hours
     Voltage: 100V
Blocking:
     Blocking Solution: 5% milk in 1X TBST
     Time: 1 hour at room temperature
Primary Antibody:
     Dilution: 1/200
     Diluent Buffer: 2.5% BSA
     Incubation Time: overnight
     Incubation Temperature: 4℃
Washing Conditions:
     Wash Solution: 1X TBST
     Time and Repetitions: 5 min each for 3 times
Secondary Antibody
Manufacturer and Catalog #: Thermo Scientific, cat # 34095, Lot # SG251180
Secondary description: goat anti-rat secondary antibody
     Dilution: 1/5000
     Diluent Buffer: 3% milk
     Incubation Time: 1 hour
     Incubation Temperature: room temperature
Detection Method:
Detection: ECL (GE, cat # RPN2209, lot #n9838243)
Procedure: Add equal volume of A and B, mix and apply on the membranes for 3-5 min before exposure
Development Time: 25 seconds
Molecular weight of band(s): ~ 38, 43 kDa
Experimental Concerns and Observations: Specific bands around 38, 43 kDa were observed
Western Blot Caspase-11 NB120-10454
Enlarge
5
reviewed by:
Anonymous
WB Human 08/06/2016
View

Summary

ApplicationWestern Blot
SpeciesHuman
LotPO number: P00129026
FileView PDF
Western Blot Caspase-11 NB120-10454
Enlarge
4
reviewed by:
Anonymous
WB Mouse 02/02/2016
View

Summary

ApplicationWestern Blot
Sample Testedmouse macrophage
SpeciesMouse
LotA-6

Blocking

Blocking Details5% skim milk in PBS-T buffer

Primary Anitbody

Dilution Ratio4 degree overnight

Secondary Antibody

Secondary DescriptionGoat anti-rat IgG, HRP (Abcam)
Secondary Concentration1:4,000

Details

Detection NotesECL

Comments

Commentsthis is a very good antibody for pro-Caspase 11 detection, its specificity against the activated form of caspase 11 remains unknown, at least we didn't see a strong potency for the detection of activated form through western blot.
Western Blot Caspase-11 NB120-10454
Enlarge
4
reviewed by:
Anonymous
WB Mouse 03/19/2015
View

Summary

ApplicationWestern Blot
Sample TestedBone Marrrow Derived Macrophages
SpeciesMouse
Lot102M4832

Blocking

Blocking Details5% Milk

Primary Anitbody

Dilution Ratio1:1000; overnight; 4C, 2.5%Milk/TBST

Secondary Antibody

Secondary DescriptionGoat anti-Rat
Secondary Concentration1:10000

Details

Detection NotesHRP, 3min, LPS untreated negative control
Western Blot Caspase-11 NB120-10454
Enlarge
4
reviewed by:
Hyun Yong Jin
WB Mouse 07/01/2014
View

Summary

ApplicationWestern Blot
SpeciesMouse

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Caspase-11 Antibody (NB120-10454). (Showing 1 - 2 of 2 FAQs).

  1. If I dilute the 100 uL of antibody (the whole delivered content) in 10 mL of buffer to incubate my blottet membrane in (and I need this volume of about 10 mL for the membrane to float in it on the shaker). I will be able to re-use this one dilution (which is 10 ug/mL) for 100 western blots? So that means for 100 samples? Or even for 100 membranes? Usually we use a antibody-dilution of 1:1000 (around 1ug/mL), this would be 1:100 (10ug/mL).
    • I am hoping I can assist you in determining usage of this antibody in your assay. The antibody comes at a starting concentration of 1 mg/mL. Since you get 100uL in the vial you will have 100 ug of antibody. The lab recommends using a dilution of 1:100-1:200 for WB. Therefore if you wished to use 10 mL volume on your incubation you would need to add 100 uL of antibody into 9.9mL of buffer to give you a final concentration of 10 ug/uL. Since you are using such a large final volume you will only get 1-2 blots per Western ran since we do not recommend reusing your antibody. If you can run the blots with much lower final volume such as 1 mL instead you can get away with a lot more Westerns.
  2. If it has been determined, can you let me know as specifically as possible the epitope that the Caspase-11 antibody recognizes, eg. peptide sequence or approximate region of the p30 subunit?
    • This antibody has not been epitope mapped and was generated using full-length p30. The only information we have is that the antibody may be used for immunoblotting [to detect the full length caspase 11 (apparent size of 35-43 kDa), and the p10 and p30fragments], so it must recognize a region similar in both subunits.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB120-10454AF350
Alexa Fluor 405 NB120-10454AF405
Alexa Fluor 488 NB120-10454AF488
Alexa Fluor 532 NB120-10454AF532
Alexa Fluor 594 NB120-10454AF594
Alexa Fluor 647 NB120-10454AF647
Alexa Fluor 700 NB120-10454AF700
Alexa Fluor 750 NB120-10454AF750
Biotin NB120-10454B
DyLight 350 NB120-10454UV
DyLight 405 NB120-10454V
DyLight 488 NB120-10454G
DyLight 550 NB120-10454R
DyLight 594 NB120-10454DL594
DyLight 650 NB120-10454C
DyLight 680 NB120-10454FR
DyLight 755 NB120-10454IR
FITC NB120-10454F
HRP NB120-10454H
Janelia Fluor 549 NB120-10454JF549
Janelia Fluor 646 NB120-10454JF646

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Recent Reviews

4
5
1
4
4
3
1
2
0
1
0

 
Anonymous
03/31/2018
Application: WB
Species: Mouse

Anonymous
12/18/2017
Application: WB
Species: Mouse

Anonymous
08/06/2016
Application: WB
Species: Human

Bioinformatics

Gene Symbol Casp4
Uniprot