Caspase-11 Antibody (17D9)


Western Blot: Caspase-11 Antibody (17D9) [NB120-10454] - Analysis of Caspase-11 in THP-1 cell lysates that were untreated (left lane) or treated (right lane) with 100 ng/ml LPS. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Caspase 11 Antibody (17D9) [NB120-10454] - Caspase 11 antibody was tested in NIH-3T3 cells at a 1:100 dilution using a Dylight 488 conjugated secondary antibody (Green). Actin more
Immunohistochemistry-Paraffin: Caspase 11 Antibody (17D9) [NB120-10454] - IHC-P analysis of a formalin fixed paraffin embedded tissue section of mouse lung using Caspase 11 antibody (clone 17D9) at 1:100 diluton. The more
Western Blot: Caspase 11 Antibody (17D9) [NB120-10454] - Mouse B cell. Image from verified customer review.
Western Blot: Caspase 11 Antibody (17D9) [NB120-10454] - Mouse liver extract was separated on a 4-15% gel and transfered to PVDF membrane. The membrane was probed with anti-caspase 11 antibody and detected with an more
Western Blot: Caspase 11 Antibody (17D9) [NB120-10454] - analysis of Caspase 11 in LPS stimulated bone marrow derived macrophages using anti-Caspase 11 antibody. Image from verified customer review.

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, MiAr
1.0 mg/ml

Caspase-11 Antibody (17D9) Summary

A recombinant p30 subunit of mouse Caspase 11. [UniProt# P70343]
Cytoplasmic (Endoplasmic reticulum membrane and Mitochondrion)
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1.0 mg/ml
Protein G purified


  • Western Blot 5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation
  • Microarray
Application Notes
Use in immunprecipitation reported in scientific literature (PMID 10791975) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
35-43 kDa
Reviewed Applications
Read 4 Reviews rated 4.3
NB120-10454 in the following applications:

Read Publications using
NB120-10454 in the following applications:

  • 1 publication
  • IHC
    2 publications
  • IP
    3 publications
  • WB
    15 publications


Caspases are cysteine proteases that play an essential role in apoptosis; their regulation is post-translational to enable rapid activation. Murine Caspase 11, also known as Caspase 4 or ICH3, has a heterotetrameric structure consisting of two anti-parallel heterodimers, each one formed of a large (p20) and a small (p10) subunit. Each heterodimer has a weight of 43kDa. Upon activation, murine caspase 11 is cleaved by an autocatalytic mechanism to give rise to the individual subunits. Expression levels of murine Caspase 11 are highest in lung and spleen.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, ICC/IF, IHC, IHC-P
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Applications: WB, IHC, IHC-P, IP
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Species: Hu
Applications: WB
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IF
Species: Hu, Mu
Applications: WB, Simple Western, IP
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, MiAr

Publications for Caspase-11 Antibody (NB120-10454)(18)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ELISA, IHC, IP, WB.

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Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using NB120-10454 Applications Species
Kim MJ, Bae SH, Ryu JC et al. SESN2/sestrin2 suppresses sepsis by inducing mitophagy and inhibiting NLRP3 activation in macrophages. Autophagy Jun 23 2016 12:00AM [PMID:27337507] (WB, IP) WB, IP
Kim MJ, Bae SH, Ryu JC et al. SESN2/sestrin2 suppresses sepsis by inducing mitophagy and inhibiting NLRP3 activation in macrophages. Autophagy 2016 Jun 23 [PMID:27337507] (WB, IP) WB, IP
Chung H, Vilaysane A, Lau A et al. NLRP3 regulates a non-canonical platform for caspase-8 activation during epithelial cell apoptosis. Cell Death Differ. 2016 Feb 19 [PMID:26891693] (WB, Mouse) WB Mouse
Kayagaki N, Stowe IB, Lee BL et al. Caspase-11 cleaves gasdermin D for non-canonical inflammasome signaling. Nature 2015 Sep 16 [PMID:26375259] (WB, Mouse) WB Mouse
Shi J, Zhao Y, Wang K et al. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death. Nature 2015 Sep 16 [PMID:26375003] (WB, Mouse) WB Mouse
Aachoui Y, Kajiwara Y, Leaf IA et al. Canonical Inflammasomes Drive IFN-gamma to Prime Caspase-11 in Defense against a Cytosol-Invasive Bacterium. Cell Host Microbe 2015 Sep 09 [PMID:26320999] (WB, Mouse) WB Mouse
Diabate M, Munro P, Garcia E et al. Escherichia coli a-Hemolysin Counteracts the Anti-Virulence Innate Immune Response Triggered by the Rho GTPase Activating Toxin CNF1 during Bacteremia. PLoS Pathog. 2015 Mar 01 [PMID:25781937] (ELISA, Mouse) ELISA Mouse
Youm YH, Nguyen KY, Grant RW et al. The ketone metabolite beta-hydroxybutyrate blocks NLRP3 inflammasome-mediated inflammatory disease Nat. Med. 2015 Feb 16 [PMID:25686106] (WB, Human) WB Human
Yuan Z, Syed MA, Panchal D et al. TREM-1 mediated Bcl-2 induction prolongs macrophage survival. J. Biol. Chem. 2014 Apr 07 [PMID:24711453] (WB, Mouse) WB Mouse
Van Opdenbosch Nina, Gurung Prajwal, Vande Walle Lieselotte et al. Activation of the NLRP1b inflammasome independently of ASC-mediated caspase-1 autoproteolysis and speck formation. Nat Commun. 2014 [PMID:24492532] (WB) WB
Show All 18 Publications.

Reviews for Caspase-11 Antibody (NB120-10454) (4) 4.34

Average Rating: 4.3
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, Mouse.
We have 4 review tested in 1 application: WB.

Reviews using NB120-10454:
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Images Ratings Applications Species Date Details
Western Blot Caspase-11 NB120-10454
reviewed by:
WB Human 08/06/2016


ApplicationWestern Blot
LotPO number: P00129026
FileView PDF
Western Blot Caspase-11 NB120-10454
reviewed by:
WB Mouse 02/02/2016


ApplicationWestern Blot
Sample Testedmouse macrophage


Blocking Details5% skim milk in PBS-T buffer

Primary Anitbody

Dilution Ratio4 degree overnight

Secondary Antibody

Secondary DescriptionGoat anti-rat IgG, HRP (Abcam)
Secondary Concentration1:4,000


Detection NotesECL


Commentsthis is a very good antibody for pro-Caspase 11 detection, its specificity against the activated form of caspase 11 remains unknown, at least we didn't see a strong potency for the detection of activated form through western blot.
Western Blot Caspase-11 NB120-10454
reviewed by:
WB Mouse 03/19/2015


ApplicationWestern Blot
Sample TestedBone Marrrow Derived Macrophages


Blocking Details5% Milk

Primary Anitbody

Dilution Ratio1:1000; overnight; 4C, 2.5%Milk/TBST

Secondary Antibody

Secondary DescriptionGoat anti-Rat
Secondary Concentration1:10000


Detection NotesHRP, 3min, LPS untreated negative control
Western Blot Caspase-11 NB120-10454
reviewed by:
WB Mouse 07/01/2014


ApplicationWestern Blot

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Caspase-11 Antibody (NB120-10454). (Showing 1 - 2 of 2 FAQs).

  1. If I dilute the 100 uL of antibody (the whole delivered content) in 10 mL of buffer to incubate my blottet membrane in (and I need this volume of about 10 mL for the membrane to float in it on the shaker). I will be able to re-use this one dilution (which is 10 ug/mL) for 100 western blots? So that means for 100 samples? Or even for 100 membranes? Usually we use a antibody-dilution of 1:1000 (around 1ug/mL), this would be 1:100 (10ug/mL).
    • I am hoping I can assist you in determining usage of this antibody in your assay. The antibody comes at a starting concentration of 1 mg/mL. Since you get 100uL in the vial you will have 100 ug of antibody. The lab recommends using a dilution of 1:100-1:200 for WB. Therefore if you wished to use 10 mL volume on your incubation you would need to add 100 uL of antibody into 9.9mL of buffer to give you a final concentration of 10 ug/uL. Since you are using such a large final volume you will only get 1-2 blots per Western ran since we do not recommend reusing your antibody. If you can run the blots with much lower final volume such as 1 mL instead you can get away with a lot more Westerns.
  2. If it has been determined, can you let me know as specifically as possible the epitope that the Caspase-11 antibody recognizes, eg. peptide sequence or approximate region of the p30 subunit?
    • This antibody has not been epitope mapped and was generated using full-length p30. The only information we have is that the antibody may be used for immunoblotting [to detect the full length caspase 11 (apparent size of 35-43 kDa), and the p10 and p30fragments], so it must recognize a region similar in both subunits.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB120-10454AF405
Alexa Fluor (R) 488 Labeled NB120-10454AF488
Alexa Fluor (R) 647 Labeled NB120-10454AF647
Alexa Fluor (R) 700 Labeled NB120-10454AF700
Allophycocyanin Labeled NB120-10454APC
Biotin Labeled NB120-10454B
DyLight 350 Labeled NB120-10454UV
DyLight 405 Labeled NB120-10454V
DyLight 405LS Labeled NB120-10454V3
DyLight 488 Labeled NB120-10454G
DyLight 550 Labeled NB120-10454R
DyLight 650 Labeled NB120-10454C
DyLight 680 Labeled NB120-10454FR
DyLight 755 Labeled NB120-10454IR
HRP Labeled NB120-10454H
PE Labeled NB120-10454PE
PerCP Labeled NB120-10454PCP

Additional Caspase-11 Antibody (17D9) Products

Caspase-11 NB120-10454

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Bioinformatics Tool for Caspase-11 Antibody (NB120-10454)

Discover related pathways, diseases and genes to Caspase-11 Antibody (NB120-10454). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Caspase-11.

Caspase 11 - A proinflammatory caspase that induces the innate immune response
While known for their role in programmed cell death, caspases are also essential for mediating inflammatory responses and innate immunity. Binding of microbial molecules by pattern recognition receptors triggers the formation of the multiprotein in...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol CASP11

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