C-Peptide Antibody (HL1159) - Azide and BSA Free

Immunohistochemistry-Paraffin: C-Peptide Antibody (HL1159) - Azide and BSA Free [NBP3-25422] - C-Peptide antibody [HL1159] detects secreted C-Peptide protein by immunohistochemical analysis. Sample: Paraffin-embedded human pancreas. C-Peptide stained by C-Peptide antibody [HL1159] (NBP3-25422) diluted at 1:10000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: C-Peptide Antibody (HL1159) - Azide and BSA Free [NBP3-25422] - C-Peptide antibody [HL1159] detects C-Peptide protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. C-Peptide stained by C-Peptide antibody [HL1159] (NBP3-25422) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

C-Peptide antibody [HL1159] detects C-Peptide protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse pancreas.Green: C-Peptide stained by C-Peptide antibody [HL1159] (NBP3-25422) diluted at 1:500.Blue: Fluoroshield with DAPI .

C-Peptide antibody [HL1159] detects secreted C-Peptide protein by immunohistochemical analysis.Sample: Paraffin-embedded rat pancreas.C-Peptide stained by C-Peptide antibody [HL1159] (NBP3-25422) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Indirect ELISA analysis was performed by coating the plate with recombinant mature C-Peptide protein (19.05-0.3 nM). Coated protein was probed with C-Peptide antibody [HL1159] (NBP3-25422) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.

C-Peptide antibody [HL1159] detects secreted C-Peptide protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse pancreas.C-Peptide stained by C-Peptide antibody [HL1159] (NBP3-25422) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt, Pm
• Applications: ELISA, IHC
• Clone: HL1159
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

C-Peptide Antibody (HL1159) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit C-Peptide Antibody (HL1159) - Azide and BSA Free (NBP3-25422) is a recombinant monoclonal antibody validated for use in IHC and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide corresponding to human C-Peptide.
• Source: HEK293
• Predicted Species: Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: INS
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA Assay dependent
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:10000
• Theoretical MW: 12 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for C-Peptide Antibody (HL1159) - Azide and BSA Free

• C-P
• C-Peptide
• IDDM2
• ILPR
• Insulin C-Peptide
• insulin
• IRDN
• MODY10
• Proinsulin C-Peptide
• proinsulin

Background

C Peptide is part of the molecule of Proinsulin, that consists of three parts: C Peptide and two long strands of amino acids (called the alpha and beta chains) that later become linked together to form the insulin molecule. From every molecule of proinsulin, one molecule of insulin plus one molecule of C Peptide are produced. C peptide is released into the blood stream in equal amounts to insulin. A test of C peptide levels will show how much insulin the body is making. Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: INS