This protein is not active and should not be used for experiments requiring activity.
>80% by SDS-PAGE and Coomassie blue staining
Product may contain endotoxins and is not suitable for use with live cells.
75.2 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.
FAQs for c-Myc Recombinant Protein (H00004609-P01). (Showing 1 - 4 of 4 FAQs).
Does the product have any other tags (e.g. 6xHIS) other than the GST-tag?
This protein only has an N-Terminal GST tag. There are no other epitope tags.
What is the concentration of the protein provided in the buffered solution? If it varies with Lot number, could you please give an indicative concentration, as no concentration information is available via your website and I'm unsure whether the stock concentration will be suitable for my assays. All I can see is that the quantity provided is 10ug.
The concentration ranges for this protein usually vary from 0.01-0.1mg/ml.
The product is listed as "not active, and should not be used for experiments requiring activity". However, do you have any evidence that this protein retains the ability to interact with known protein partners (e.g. Max)?
This protein has only been tested as a positive control in Western blot and ELISA. It has not yet been tested for any possible interactions with other related proteins. A cell free wheat germ system is utelized for their protein expression. Due to this expression system, in our experience, the proteins do not always undergo the same folding and post-translational modifications they might undergo in an endogenous cell. This is why we do not recommend this protein for use in functional assays and do not guarantee them for this use.
What is the degree of purity for the 3 products H00004609-P01, H00004602-P01, and H00004609-Q01?Fragment-->
All of these proteins are produced in Host: Wheat Germ (in vitro), Preparation Method:in vitro wheat germ expression system, Purification:Glutathione Sepharose 4 Fast Flow.
c-Myc - transcription factor and oncogene c-Myc is a protein of the Myc family of transcription factors (c-Myc, B-Myc, L-Myc, N-Myc, and s-Myc) encoded by the MYC proto-oncogene. c-Myc was first discovered as the cellular homolog of the retroviral v-Myc oncogene. c-Myc is a transcription... Read full blog post.
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The Myc Epitope Tags Along... Recombinant DNA technology allows researchers to fuse epitope tags to their protein of interest and then identify that protein using tag specific antibodies. The Myc Tag can be used to purify tagged proteins by affinity chromatography or detect them b... Read full blog post.
Epitope Tags and the V5 Tag Antibody We at Novus Biologicals have a large antibody catalog targeted to epitope tags. They include C-Myc, GFP, HA, DYKDDDDK, and V5 Tag antibody reagents. The V5 tag antibody recognizes the artificially engineered V5 epitope, which is formed of amino acid r... Read full blog post.
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